Cytochrome d but not cytochrome o rescues the toluidine blue growth sensitivity of arc mutants of Escherichia coli.

Abstract:

:The Arc (anoxic redox control) two-component signal transduction system, consisting of the ArcB sensor kinase and the ArcA response regulator, allows adaptive responses of Escherichia coli to changes of O(2) availability. The arcA gene was previously known as the dye gene because null mutants were growth sensitive to the photosensitizer redox dyes toluidine blue and methylene blue, a phenotype whose molecular basis still remains elusive. In this study we report that the toluidine blue O (TBO) effect on the arc mutants is light independent and observed only during aerobic growth conditions. Moreover, 16 suppressor mutants with restored growth were generated and analyzed. Thirteen of those possessed insertion elements upstream of the cydAB operon, rendering its expression ArcA independent. Also, it was found that, in contrast to cythocrome d, cythocrome o was not able to confer toluidine blue resistance to arc mutants, thereby representing an intriguing difference between the two terminal oxidases. Finally, a mechanism for TBO sensitivity and resistance is discussed.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Alvarez AF,Malpica R,Contreras M,Escamilla E,Georgellis D

doi

10.1128/JB.00881-09

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

391-9

issue

2

eissn

0021-9193

issn

1098-5530

pii

JB.00881-09

journal_volume

192

pub_type

杂志文章
  • Isolation, phenotypic characterization, and complementation analysis of mutants of Methylobacterium extorquens AM1 unable to synthesize pyrroloquinoline quinone and sequences of pqqD, pqqG, and pqqC.

    abstract::Aerobic gram-negative methylotrophs oxidize methanol to formaldehyde by using a methanol dehydrogenase that has pyrroloquinoline quinone (PQQ) as a prosthetic group. Seventy-two mutants which are unable to grow on methanol unless the growth medium is supplemented with PQQ have been isolated in the facultative methanol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.6.1746-1755.1994

    authors: Morris CJ,Biville F,Turlin E,Lee E,Ellermann K,Fan WH,Ramamoorthi R,Springer AL,Lidstrom ME

    更新日期:1994-03-01 00:00:00

  • Activators of the glutamate-dependent acid resistance system alleviate deleterious effects of YidC depletion in Escherichia coli.

    abstract::The function of the essential inner membrane protein (IMP) YidC in Escherichia coli has been studied for a limited number of model IMPs and primarily using targeted approaches. These studies suggested that YidC acts at the level of insertion, folding, and quality control of IMPs, both in the context of the Sec translo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01209-10

    authors: Yu Z,Bekker M,Tramonti A,Cook GM,van Ulsen P,Scheffers DJ,de Mattos JT,De Biase D,Luirink J

    更新日期:2011-03-01 00:00:00

  • Reduce, Induce, Thrive: Bacterial Redox Sensing during Pathogenesis.

    abstract::The abundance of oxidants and reductants must be balanced for an organism to thrive. Bacteria have evolved methods to prevent redox imbalances and to mitigate their deleterious consequences through the expression of detoxification enzymes, antioxidants, and systems to repair or degrade damaged proteins and DNA. Regula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00128-18

    authors: Reniere ML

    更新日期:2018-08-10 00:00:00

  • Isolation and characterization of Escherichia coli tolC mutants defective in secreting enzymatically active alpha-hemolysin.

    abstract::This study describes the isolation and characterization of a unique class of TolC mutants that, under steady-state growth conditions, secreted normal levels of largely inactive alpha-hemolysin. Unlike the reduced activity in the culture supernatants, the cell-associated hemolytic activity in these mutants was identica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.23.6908-6916.2001

    authors: Vakharia H,German GJ,Misra R

    更新日期:2001-12-01 00:00:00

  • CfaD-dependent expression of a novel extracytoplasmic protein from enterotoxigenic Escherichia coli.

    abstract::H10407 is a strain of enterotoxigenic Escherichia coli (ETEC) that utilizes CFA/I pili to adhere to surfaces of the small intestine, where it elaborates toxins that cause profuse watery diarrhea in humans. Expression of the CFA/I pilus is positively regulated at the level of transcription by CfaD, a member of the AraC...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00131-07

    authors: Pilonieta MC,Bodero MD,Munson GP

    更新日期:2007-07-01 00:00:00

  • Spontaneous deletion of citrate-utilizing ability promoted by insertion sequences.

    abstract::The citrate utilization (Cit+) transposon Tn3411 was shown to be flanked by directly repeated sequences (IS3411L and IS3411R) by restriction enzyme analysis and electron microscope observation. Cit- deletion mutants were frequently found to be generated in pBR322::Tn3411 by intramolecular recombination between the two...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.2.642-650.1984

    authors: Ishiguro N,Sato G

    更新日期:1984-11-01 00:00:00

  • Identification of a novel matrix protein that promotes biofilm maturation in Vibrio fischeri.

    abstract::Bacteria form communities, termed biofilms, in which cells adhere to each other within a matrix, typically comprised of polysaccharides, proteins, and extracellular DNA. Biofilm formation by the marine bacterium Vibrio fischeri requires the Syp polysaccharide, but the involvement of matrix proteins is as yet unknown. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02292-14

    authors: Ray VA,Driks A,Visick KL

    更新日期:2015-02-01 00:00:00

  • Extracellular collagenase from Trichophyton schoenleinii.

    abstract::The dermatophyte Trichophyton schoenleinii elaborates an extracellular collagenase which is specific for native collagen and gelatin and is separable from an enzyme which is active against Azocoll. The fungal enzyme differs from the bacterial collagenases in its acid pH and small molecular weight. In addition, the fun...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.1.43-46.1968

    authors: Rippon JW

    更新日期:1968-01-01 00:00:00

  • Pseudomonas stutzeri and related species undergo natural transformation.

    abstract::Cells of Pseudomonas stutzeri are naturally transformed by homologous chromosomal DNA; they do not require chemical treatment to become competent. This capacity to undergo natural transformation was found to be shared by the closely related species P. mendocina, P. alcaligenes, and P. pseudoalcaligenes, but was not de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.1.93-99.1983

    authors: Carlson CA,Pierson LS,Rosen JJ,Ingraham JL

    更新日期:1983-01-01 00:00:00

  • Characterization of mutations in the metY-nusA-infB operon that suppress the slow growth of a DeltarimM mutant.

    abstract::The RimM protein in Escherichia coli is associated with free 30S ribosomal subunits but not with 70S ribosomes. A DeltarimM mutant shows a sevenfold-reduced growth rate and a reduced translational efficiency, probably as a result of aberrant assembly of the ribosomal 30S subunits. The slow growth and translational def...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.20.6095-6106.2001

    authors: Bylund GO,Lövgren JM,Wikström PM

    更新日期:2001-10-01 00:00:00

  • The CpxRA signal transduction system of Escherichia coli: growth-related autoactivation and control of unanticipated target operons.

    abstract::In Escherichia coli, the CpxRA two-component signal transduction system senses and responds to aggregated and misfolded proteins in the bacterial envelope. We show that CpxR-P (the phosphorylated form of the cognate response regulator) activates cpxRA expression in conjunction with RpoS, suggesting an involvement of t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.21.6772-6778.1999

    authors: De Wulf P,Kwon O,Lin EC

    更新日期:1999-11-01 00:00:00

  • Proteolytic adaptor for transfer-messenger RNA-tagged proteins from alpha-proteobacteria.

    abstract::We have identified an analog of SspB, the proteolytic adaptor for transfer-messenger RNA (tmRNA)-tagged proteins, in Caulobacter crescentus. C. crescentus SspB shares limited sequence similarity with Escherichia coli SspB but binds the tmRNA tag in vitro and is required for optimal proteolysis of tagged proteins in vi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01387-06

    authors: Lessner FH,Venters BJ,Keiler KC

    更新日期:2007-01-01 00:00:00

  • Inhibition of thiamine transport by chloroethylthiamine in Escherichia coli.

    abstract::Chloroethylthiamine was found to inhibit an entrapment of thiamine as thiamine monophosphate by blocking thiamine monophosphokinase in the cytoplasm after thiamine was taken up by the cells of Escherichia coli. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.3.1438-1440.1972

    authors: Iwashima A,Nose Y

    更新日期:1972-12-01 00:00:00

  • Pesticins. II. Production of pesticin I and II.

    abstract::Brubaker, Robert R. (Fort Detrick, Frederick, Md.) and Michael J. Surgalla. Pesticins. II. Production of pesticin I and II. J. Bacteriol. 84:539-545. 1962.-Pesticin I was separated from pesticin I inhibitor by ion-exchange chromatography of cell-free culture supernatant fluids and by acid precipitation of soluble prep...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.3.539-545.1962

    authors: BRUBAKER RR,SURGALLA MJ

    更新日期:1962-09-01 00:00:00

  • Cyanobacterial transposons Tn5469 and Tn5541 represent a novel noncomposite transposon family.

    abstract::A noncomposite transposon, designated Tn5541, was isolated from strain Fd33 of the filamentous cyanobacterium Fremyella diplosiphon UTEX 481. Sequence analysis showed that Tn5541 is structurally and genetically very similar to Tn5469, which is also endogenous to F. diplosiphon. Both Tn5469 and Tn5541 encode homologous...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.22.6059-6063.1998

    authors: Schaefer MR,Kahn K

    更新日期:1998-11-01 00:00:00

  • Isolation and characterization of catabolite repression control mutants of Pseudomonas aeruginosa PAO.

    abstract::Independently controlled, inducible, catabolic genes in Pseudomonas aeruginosa are subject to strong catabolite repression control by intermediates of the tricarboxylic acid cycle. Mutants which exhibited a pleiotropic loss of catabolite repression control of multiple pathways were isolated. The mutations mapped in th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.15.4700-4706.1991

    authors: Wolff JA,MacGregor CH,Eisenberg RC,Phibbs PV Jr

    更新日期:1991-08-01 00:00:00

  • Functional relationship among the gene clusters encoding F7(1), F7(2), F9, and F11 fimbriae of human uropathogenic Escherichia coli.

    abstract::The P fimbrial gene clusters encoding the serologically different F7(1), F7(2), F9, and F11 fimbriae were compared functionally. The results show that these gene clusters are closely related. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.1.407-410.1986

    authors: Van Die I,Van Megen I,Zuidweg E,Hoekstra W,De Ree H,Van den Bosch H,Bergmans H

    更新日期:1986-07-01 00:00:00

  • Regulation of a phage endolysin by disulfide caging.

    abstract::In contrast to canonical phage endolysins, which require holin-mediated disruption of the membrane to gain access to attack the cell wall, signal anchor release (SAR) endolysins are secreted by the host sec system, where they accumulate in an inactive form tethered to the membrane by their N-terminal SAR domains. SAR ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00674-10

    authors: Kuty GF,Xu M,Struck DK,Summer EJ,Young R

    更新日期:2010-11-01 00:00:00

  • Stable isotope peptide mass spectrometry to decipher amino acid metabolism in Dehalococcoides strain CBDB1.

    abstract::Dehalococcoides species are key players in the anaerobic transformation of halogenated solvents at contaminated sites. Here, we analyze isotopologue distributions in amino acid pools from peptides of Dehalococcoides strain CBDB1 after incubation with (13)C-labeled acetate or bicarbonate as a carbon source. The resulti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00049-12

    authors: Marco-Urrea E,Seifert J,von Bergen M,Adrian L

    更新日期:2012-08-01 00:00:00

  • Diversity of genome structure in Salmonella enterica serovar Typhi populations.

    abstract::The genomes of most strains of Salmonella and Escherichia coli are highly conserved. In contrast, all 136 wild-type strains of Salmonella enterica serovar Typhi analyzed by partial digestion with I-CeuI (an endonuclease which cuts within the rrn operons) and pulsed-field gel electrophoresis and by PCR have rearrangeme...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.8.2638-2650.2005

    authors: Kothapalli S,Nair S,Alokam S,Pang T,Khakhria R,Woodward D,Johnson W,Stocker BA,Sanderson KE,Liu SL

    更新日期:2005-04-01 00:00:00

  • Inhibition of the development of the spore septum and membranes in Bacillus cereus by beta-phenethyl alcohol.

    abstract::Remsen, C. C. (Syracuse University, Syracuse, N.Y.), D. G. Lundgren, and R. A. Slepecky. Inhibition of the development of the spore septum and membranes in Bacillus cereus by beta-phenethyl alcohol. J. Bacteriol. 91:324-331. 1966.-The effect of phenethyl alcohol (PEA) upon the initial stages of sporulation in Bacillus...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.1.324-331.1966

    authors: Remsen CC,Lundgren DG,Slepecky RA

    更新日期:1966-01-01 00:00:00

  • Differences in cold adaptation of Bacillus subtilis under anaerobic and aerobic conditions.

    abstract::Bacillus subtilis, which grows under aerobic conditions, employs fatty acid desaturase (Des) to fluidize its membrane when subjected to temperature downshift. Des requires molecular oxygen for its activity, and its expression is regulated by DesK-DesR, a two-component system. Transcription of des is induced by the tem...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00384-10

    authors: Beranová J,Mansilla MC,de Mendoza D,Elhottová D,Konopásek I

    更新日期:2010-08-01 00:00:00

  • Genomic analysis of Rickettsia japonica strain YHT.

    abstract::Rickettsia japonica strain YH, isolated in 1984 in Japan, is the type strain of R. japonica, the tick-borne agent of Japanese spotted fever. Here, we report the 1.33-Mb genome of this rickettsial species. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01928-12

    authors: Dong X,El Karkouri K,Robert C,Raoult D,Fournier PE

    更新日期:2012-12-01 00:00:00

  • Caulobacter crescentus pili: structure and stage-specific expression.

    abstract::Pili are functionally expressed during the predivisional and swarmer stages of the Caulobacter crescentus differentiation cycle. They appear on the developing swarmer pole and at the same cellular location as flagella and the phiCbK receptor sites. Pili disappear when the swarmer cell differentiates into a stalked cel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.131.1.340-346.1977

    authors: Lagenaur C,Agabian N

    更新日期:1977-07-01 00:00:00

  • The presence of a defective LEU2 gene on 2 mu DNA recombinant plasmids of Saccharomyces cerevisiae is responsible for curing and high copy number.

    abstract::The copy number of 2 mu DNA-derived plasmids in CIR+ Saccharomyces cerevisiae transformants is determined by its selective marker and is usually much lower than that of the endogenous plasmid. Only plasmids containing the leu2 allele of pJDB219, designated as leu2-d, under selective conditions displayed a higher copy ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.2.625-635.1983

    authors: Erhart E,Hollenberg CP

    更新日期:1983-11-01 00:00:00

  • Role of Na+ cycle in cell volume regulation of Mycoplasma gallisepticum.

    abstract::The mechanism for the extrusion of Na+ from Mycoplasma gallisepticum cells was examined. Na+ efflux from cells was studied by diluting 22Na+-loaded cells into an isoosmotic NaCl solution and measuring the residual 22Na+ in the cells. Uphill 22Na+ efflux was found to be glucose dependent and linear with time over a 60-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.8.4410-4416.1989

    authors: Shirvan MH,Schuldiner S,Rottem S

    更新日期:1989-08-01 00:00:00

  • Reconstitution of signaling in bacterial chemotaxis.

    abstract::Strains missing several genes required for chemotaxis toward amino acids, peptides, and certain sugars were tethered and their rotational behavior was analyzed. Null strains (called gutted) were deleted for genes that code for the transducers Tsr, Tar, Tap, and Trg and for the cytoplasmic proteins CheA, CheW, CheR, Ch...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.5.1878-1885.1987

    authors: Wolfe AJ,Conley MP,Kramer TJ,Berg HC

    更新日期:1987-05-01 00:00:00

  • Molecular cloning of the actinomycin synthetase gene cluster from Streptomyces chrysomallus and functional heterologous expression of the gene encoding actinomycin synthetase II.

    abstract::The actinomycin synthetases ACMS I, II, and III catalyze the assembly of the acyl peptide lactone precursor of actinomycin by a nonribosomal mechanism. We have cloned the genes of ACMS I (acmA) and ACMS II (acmB) by hybridization screening of a cosmid library of Streptomyces chrysomallus DNA with synthetic oligonucleo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.9.2468-2474.1998

    authors: Schauwecker F,Pfennig F,Schröder W,Keller U

    更新日期:1998-05-01 00:00:00

  • Adenosine 3',5'-phosphate phosphodiesterase and pheromone response in the yeast Saccharomyces cerevisiae.

    abstract::Theophylline, aminophylline, and isobutylmethylxanthine, compounds reported to be inhibitors of adenosine 3',5'-phosphate (cAMP) phosphodiesterase, prevented the alpha-factor-induced cell cycle arrest of Saccharomyces cerevisiae a cells. To determine whether the in vivo effect of these methylxanthines on yeast pheromo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.148.3.919-925.1981

    authors: Liao HH,Thorner J

    更新日期:1981-12-01 00:00:00

  • In vivo rearrangement of foreign DNA by Fusarium oxysporum produces linear self-replicating plasmids.

    abstract::Particular combinations of fungal strains and transformation vectors allow for fungal rearrangement of normally integrative plasmids, resulting in the creation of linear self-replicating plasmids in Fusarium oxysporum. The rearrangement results in the addition of fungal DNA, including telomere consensus sequences, to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.6.3163-3171.1990

    authors: Powell WA,Kistler HC

    更新日期:1990-06-01 00:00:00