Abstract:
:The actinomycin synthetases ACMS I, II, and III catalyze the assembly of the acyl peptide lactone precursor of actinomycin by a nonribosomal mechanism. We have cloned the genes of ACMS I (acmA) and ACMS II (acmB) by hybridization screening of a cosmid library of Streptomyces chrysomallus DNA with synthetic oligonucleotides derived from peptide sequences of the two enzymes. Their genes were found to be closely linked and are arranged in opposite orientations. Hybridization mapping and partial sequence analyses indicate that the gene of an additional peptide synthetase, most likely the gene of ACMS III (acmC), is located immediately downstream of acmB in the same orientation. The protein sequence of ACMS II, deduced from acmB, shows that the enzyme contains two amino acid activation domains, which are characteristic of peptide synthetases, and an additional epimerization domain. Heterologous expression of acmB from the mel promoter of plasmid PIJ702 in Streptomyces lividans yielded a functional 280-kDa peptide synthetase which activates threonine and valine as enzyme-bound thioesters. It also catalyzes the dipeptide formation of threonyl-L-valine, which is epimerized to threonyl-D-valine. Both of these dipeptides are enzyme bound as thioesters. This catalytic activity is identical to the in vitro activity of ACMS II from S. chrysomallus.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Schauwecker F,Pfennig F,Schröder W,Keller Udoi
10.1128/JB.180.9.2468-2474.1998subject
Has Abstractpub_date
1998-05-01 00:00:00pages
2468-74issue
9eissn
0021-9193issn
1098-5530journal_volume
180pub_type
杂志文章abstract::Pelosinus fermentans 16S rRNA gene sequences have been reported from diverse geographical sites since the recent isolation of the type strain. We present the genome sequence of the P. fermentans type strain R7 (DSM 17108) and genome sequences for two new strains with different abilities to reduce iron, chromate, and u...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01174-12
更新日期:2012-09-01 00:00:00
abstract::Bile acids are surface-active steroid compounds with toxic effects for bacteria. Recently, the isolation and characterization of a bacterium, Pseudomonas sp. strain Chol1, growing with bile acids as the carbon and energy source was reported. In this study, initial reactions of the aerobic degradation pathway for the b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00665-07
更新日期:2007-10-01 00:00:00
abstract::The cell surfaces of several Lactobacillus species are covered by a regular layer composed of a single species of protein, the S-protein. The 43-kDa S-protein of the neotype strain Lactobacillus acidophilus ATCC 4356, which originated from the pharynx of a human, was purified. Antibodies generated against purified S-p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.19.6089-6096.1993
更新日期:1993-10-01 00:00:00
abstract::The effects of exogenous reducing agents on a number of biological properties of purified Chlamydia trachomatis LGV-434 and Chlamydia psittaci meningopneumonitis elementary bodies (EBs) have been examined in an attempt to identify in vitro correlates of early events in the differentiation of the infectious EB to the r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.1.25-31.1985
更新日期:1985-01-01 00:00:00
abstract::Membrane fragments containing diacyl phospholipids were released from viable cells of Haemophilus parainfluenzae during incubation in ethylenediaminetetraacetic acid (EDTA)- tris(hydroxymethyl)aminomethane (Tris) buffer. The phospholipids located in the part of the membrane that was released during the EDTA-Tris treat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.103.2.329-334.1970
更新日期:1970-08-01 00:00:00
abstract::In Neurospora crassa, histidine starvation of histidine mutants resulted in derepression of histidine, tryptophan, and arginine biosynthetic enzymes. The same tripartite derepression occurred in wild-type strain 74A when it was grown in medium supplemented with 3-amino-1,2,4-triazole, an inhibitor of histidine biosynt...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.3.893-898.1974
更新日期:1974-09-01 00:00:00
abstract::The genes controlling synthesis of the Escherichia coli acidic polysaccharide capsular antigens K10 and K54 were transferred by conjugation to E. coli strains of other serotypes. The genes concerned with these K antigen determinants showed genetic linkage with the serA locus. We propose to name the K antigen-controlli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.43-51.1974
更新日期:1974-10-01 00:00:00
abstract::FK-506-binding proteins (FKBPs), which in T cells are supposed to mediate the immunosuppressive effects of the compounds FK-506 and rapamycin, have been isolated from Streptomyces chrysomallus, S. hygroscopicus subsp. ascomyceticus, and S. hygroscopicus. The latter two strains are producers of ascomycin (the ethyl ana...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.18.5888-5894.1992
更新日期:1992-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01093-12
更新日期:2012-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.5.1774-1779.1997
更新日期:1997-03-01 00:00:00
abstract::A mutant requiring isoleucine and valine for growth, because of the absence of acetolactate synthase activity, has been isolated. At least one of three different genes (ilvG, ilvB, ilvI) is required for the expression of acetolactate synthase activity, thus suggesting the presence of three different acetolactate synth...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.536-538.1974
更新日期:1974-10-01 00:00:00
abstract::A mutational change of the initiation codon to GUA was found to reduce, but not abolish, expression of the recJ gene of Escherichia coli. Specific mutations in translational initiation factor IF3 have been isolated as second-site suppressors of this GUA initiation codon mutation. One of these, infC135, with an arginin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.21.6705-6713.1997
更新日期:1997-11-01 00:00:00
abstract::The Alcaligenes eutrophus JMP134 tfdA gene, encoding the enzyme responsible for the first step in 2,4-dichlorophenoxyacetic acid (2,4-D) biodegradation, was overexpressed in Escherichia coli, and several enzymatic properties of the partially purified gene product were examined. Although the tfdA-encoded enzyme is typi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.7.2083-2086.1993
更新日期:1993-04-01 00:00:00
abstract::Many bacterial species produce capsular polysaccharides that contribute to pathogenesis through evasion of the host innate immune system. The gram-positive pathogen Enterococcus faecalis was previously reported to produce one of four capsule serotypes (A, B, C, or D). Previous studies describing the four capsule serot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00592-09
更新日期:2009-10-01 00:00:00
abstract::The position of the termination codon in lacZX90 was determined by isolation of a lac+ revertant. Lysine was found to replace tyrosine at position 1,012 of beta-galactosidase, indicating that X90 protein lacked the carboxyl-terminal 10 residues. A heat- and urea-sensitive hybrid enzyme was formed in vivo when supC, wh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.2.694-697.1981
更新日期:1981-08-01 00:00:00
abstract::Staphylococcus aureus was shown to transport iron complexed to a variety of hydroxamate type siderophores, including ferrichrome, aerobactin, and desferrioxamine. An S. aureus mutant defective in the ability to transport ferric hydroxamate complexes was isolated from a Tn917-LTV1 transposon insertion library after sel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.16.4394-4400.2000
更新日期:2000-08-01 00:00:00
abstract::Loci termed rfa, determining biosynthesis of somatic lipopolysaccharide core, have been mapped in Salmonella typhimurium LT2. The smooth-specific phage P22 co-transduced two leaky rfa alleles with cysE and with pyrE; one of the leaky alleles is perhaps rfaG, and the other is an unidentified gene concerned with synthes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.1.48-57.1972
更新日期:1972-10-01 00:00:00
abstract::A strain of Escherichia coli which was derived from a gentamicin-resistant clinical isolate was found to be cross-resistant to neomycin and streptomycin. The molecular nature of the genetic defect was found to be an insertion of two GC base pairs in the uncG gene of the mutant. The insertion led to the production of a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.3.1435-1444.1989
更新日期:1989-03-01 00:00:00
abstract::Leuconostoc mesenteroides subsp. mesenteroides is one of the most predominant lactic acid bacterial groups during kimchi fermentation. Here, we report the complete genome sequence of L. mesenteroides subsp. mesenteroides J18, which was isolated from kimchi. The genome of the strain consists of a 1,896,561-bp chromosom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06498-11
更新日期:2012-02-01 00:00:00
abstract::Pseudomonas MS can grow on methylamine and a number of other compounds containing C1 units as a sole source of carbon and energy. Assimilation of carbon into cell material occurs via the "serine pathway" since enzymes of this pathway are induced after growth on methylamine, but not malate or acetate. A mutant has been...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.3.905-910.1975
更新日期:1975-06-01 00:00:00
abstract::The control of the synthesis of certain key enzymes of aromatic amino acid biosynthesis was studied. Tyrosine represses the first enzyme of the 3-deoxy-d-arabino heptulosonic acid 7-phosphate pathway, DAHP synthetase, as well as shikimate kinase and chorismate mutase about fivefold in cultures grown under conditions l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.1.83-90.1969
更新日期:1969-01-01 00:00:00
abstract::A procedure is described for the purification of the Escherichia coli outer membrane (lipopolysaccharide or L membrane) with flagella still attached. The resulting lipopolysaccharide membrane was in the form of vesicles that had a trilaminar structure in thin section and contained about 55% lipopolysaccharide and 45% ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.396-407.1971
更新日期:1971-01-01 00:00:00
abstract::Site-directed mutagenesis has previously been used to construct Escherichia coli dnaK mutants encoding proteins that are altered at the site of in vitro phosphorylation (J. S. McCarty and G. C. Walker, Proc. Natl. Acad. Sci. USA 88:9513-9517, 1991). These mutants are unable to autophosphorylate and are severely defect...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.3.764-780.1994
更新日期:1994-02-01 00:00:00
abstract::THREE PREVIOUSLY STUDIED R FACTORS WERE USED: 222/R4, controlling transmissible resistance to sulfonamide, streptomycin, chloromycetin, and tetracycline (SU(r) SM(r) CM(r) TC(r)); 222/R3, a derivative of 222/R4 (now termed 222/R3W) having lost TC(r); and R15, controlling infectious resistance to SU and SM only. Two ty...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.103.1.166-177.1970
更新日期:1970-07-01 00:00:00
abstract::In a glycerol auxotroph of Staphylococcus aureus, the deprivation of glycerol affected the formation of certain membrane components. (i) There was synthesis of fatty acids at the predeprivation rate even though the fatty acids synthesized accumulated as free fatty acids rather than as esterified fatty acids; (ii) ther...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.1.413-420.1972
更新日期:1972-10-01 00:00:00
abstract::Lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in Escherichia coli (J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). The lrp genes from three other enteric microorganisms, Enterobacter aerogenes, Klebsiella aerogenes, and Salmonella typhimurium, were cloned and sequen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.6.1624-1626.1995
更新日期:1995-03-01 00:00:00
abstract::Type III secretion systems (T3SSs) are essential virulence devices for many gram-negative bacteria that are pathogenic for plants, animals, and humans. They serve to translocate virulence effector proteins directly into eukaryotic host cells. T3SSs are composed of a large cytoplasmic bulb and a transmembrane region in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01004-08
更新日期:2009-01-01 00:00:00
abstract::Methanohalophilus strain Z7302 was previously isolated from a hypersaline environment and grows over a range of NaCl concentrations from 1.7 to 4.4 M. We examined the relationships between cell growth rate, cell volume, and intracellular solute concentrations with increasing salinity. This extremely halophilic methano...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.22.7474-7477.1992
更新日期:1992-11-01 00:00:00
abstract::The binding of Bacteroides intermedius VPI 8944 to human fibrinogen has been characterized. The binding is time dependent, at least partially reversible, saturable, and specific. On an average, a maximum of 3,500 fibrinogen molecules bind per bacterial cell, with a dissociation constant of 1.7 X 10(-11) M. These bacte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.2.623-628.1985
更新日期:1985-08-01 00:00:00
abstract::A new bacteriocin, termed lactococcin A (LCN-A), from Lactococcus lactis subsp. cremoris LMG 2130 was purified and sequenced. The polypeptide contained no unusual amino acids and showed no significant sequence similarity to other known proteins. Only lactococci were killed by the bacteriocin. Of more than 120 L. lacti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.12.3879-3887.1991
更新日期:1991-06-01 00:00:00