Abstract:
:The cell surfaces of several Lactobacillus species are covered by a regular layer composed of a single species of protein, the S-protein. The 43-kDa S-protein of the neotype strain Lactobacillus acidophilus ATCC 4356, which originated from the pharynx of a human, was purified. Antibodies generated against purified S-protein were used to screen a lambda library containing chromosomal L. acidophilus ATCC 4356 DNA. Several phages showing expression of this S-protein in Escherichia coli were isolated. A 4.0-kb DNA fragment of one of those phages hybridized to a probe derived from an internal tryptic fragment of the S-protein. The slpA gene, coding for the surface layer protein, was located entirely on the 4.0-kb fragment as shown by deletion analysis. The nucleotide sequence of the slpA gene was determined and appeared to encode a protein of 444 amino acids. The first 24 amino acids resembled a putative secretion signal, giving rise to a mature S-protein of 420 amino acids (44.2 kDa). The predicted isoelectric point of 9.4 is remarkably high for an S-protein but is in agreement with the data obtained during purification. The expression of the entire S-protein or of large, C-terminally truncated S-proteins is unstable in E. coli.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Boot HJ,Kolen CP,van Noort JM,Pouwels PHdoi
10.1128/jb.175.19.6089-6096.1993subject
Has Abstractpub_date
1993-10-01 00:00:00pages
6089-96issue
19eissn
0021-9193issn
1098-5530journal_volume
175pub_type
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journal_title:Journal of bacteriology
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doi:10.1128/jb.174.9.2779-2784.1992
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pub_type: 杂志文章
doi:10.1128/jb.172.4.1719-1724.1990
更新日期:1990-04-01 00:00:00
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更新日期:2014-08-01 00:00:00
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更新日期:2012-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.2.279-283.1963
更新日期:1963-02-01 00:00:00
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更新日期:2003-12-01 00:00:00
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更新日期:1997-10-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1962-03-01 00:00:00
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更新日期:1966-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00