ATPase activity and oligomeric state of TrwK, the VirB4 homologue of the plasmid R388 type IV secretion system.

Abstract:

:Type IV secretion systems (T4SS) mediate the transfer of DNA and protein substrates to target cells. TrwK, encoded by the conjugative plasmid R388, is a member of the VirB4 family, comprising the largest and most conserved proteins of T4SS. VirB4 was suggested to be an ATPase involved in energizing pilus assembly and substrate transport. However, conflicting experimental evidence concerning VirB4 ATP hydrolase activity was reported. Here, we demonstrate that TrwK is able to hydrolyze ATP in vitro in the absence of its potential macromolecular substrates and other T4SS components. The kinetic parameters of its ATPase activity have been characterized. The TrwK oligomerization state was investigated by analytical ultracentrifugation and electron microscopy, and its effects on ATPase activity were analyzed. The results suggest that the hexameric form of TrwK is the catalytically active state, much like the structurally related protein TrwB, the conjugative coupling protein.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Arechaga I,Peña A,Zunzunegui S,del Carmen Fernández-Alonso M,Rivas G,de la Cruz F

doi

10.1128/JB.00321-08

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

5472-9

issue

15

eissn

0021-9193

issn

1098-5530

pii

JB.00321-08

journal_volume

190

pub_type

杂志文章
  • Distribution of the SsuDAT1I restriction-modification system among different serotypes of Streptococcus suis.

    abstract::The SsuDAT1I restriction-modification (R-M) system, which contains two methyltransferases and two restriction endonucleases with recognition sequence 5'-GATC-3', was first found in a field isolate of Streptococcus suis serotype 2. Isoschizomers of the R-M system were found in the same locus between purH and purD in a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.183.18.5436-5440.2001

    authors: Sekizaki T,Osaki M,Takamatsu D,Shimoji Y

    更新日期:2001-09-01 00:00:00

  • Site-directed mutagenesis in Escherichia coli of a stable R772::Ti cointegrate plasmid from Agrobacterium tumefaciens.

    abstract::The host range of an octopine Ti plasmid is limited to Rhizobiaceae. This has been extended also to Escherichia coli in the form of a stable cointegrate with the wide-host-range plasmid R772. Its structure was studied by constructing a physical map of R772 and of the R772::pTiB6 cointegrate. An insertion sequence pres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.2.693-701.1983

    authors: Hille J,van Kan J,Klasen I,Schilperoort R

    更新日期:1983-05-01 00:00:00

  • Macromolecular synthesis during the germanation of Saccharomyces cerevisiae spores.

    abstract::After the dormancy of Saccharomyces cerevisiae ascospores had been broken, the synthesis of proteins was observed first, followed rapidly by synthesis of ribonucleic acid (RNA) and much later by deoxyribonucleic acid (DNA) synthesis. Phosphoglucomutase activity increased in a periodic (step) fashion, whereas the activ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.3.1289-1295.1973

    authors: Rousseau P,Halvorson HO

    更新日期:1973-03-01 00:00:00

  • Phosphorylation-independent bacterial chemoresponses correlate with changes in the cytoplasmic level of fumarate.

    abstract::Bacterial chemotaxis is based on modulation of the probability to switch the direction of flagellar rotation. Responses to many stimuli are transduced by a two-component system via reversible phosphorylation of CheY, a small cytoplasmic protein that directly interacts with the switch complex at the flagellar motor. We...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.23.6882-6887.1996

    authors: Montrone M,Oesterhelt D,Marwan W

    更新日期:1996-12-01 00:00:00

  • Complete genome sequences of three Pseudomonas aeruginosa isolates with phenotypes of polymyxin B adaptation and inducible resistance.

    abstract::Clinical "superbug" isolates of Pseudomonas aeruginosa were previously observed to be resistant to several antibiotics, including polymyxin B, and/or to have a distinct, reproducible adaptive polymyxin resistance phenotype, identified by observing "skipped" wells (appearance of extra turbid wells) during broth microdi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06246-11

    authors: Boyle B,Fernandez L,Laroche J,Kukavica-Ibrulj I,Mendes CM,Hancock RW,Levesque RC

    更新日期:2012-01-01 00:00:00

  • Complete genome sequence of Helicobacter cinaedi strain PAGU611, isolated in a case of human bacteremia.

    abstract::We report the complete genome sequence of Helicobacter cinaedi strain PAGU611, isolated in a case of human bacteremia. The PAGU611 genome comprises a 2,078,348-bp chromosome and a 23,054-bp plasmid. The chromosome contains a unique genomic island, encoding a type VI secretion system and clustered regularly interspaced...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00645-12

    authors: Goto T,Ogura Y,Hirakawa H,Tomida J,Morita Y,Akaike T,Hayashi T,Kawamura Y

    更新日期:2012-07-01 00:00:00

  • DRUG RESISTANCE OF ENTERIC BACTERIA. IV. ACTIVE TRANSDUCING BACTERIOPHAGE P1 CM PRODUCED BY THE COMBINATION OF R FACTOR WITH BACTERIOPHAGE P1.

    abstract::Kondo, Eiko (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Drug resistance of enteric bacteria. IV. Active transducing phage P1 CM produced by the combination of R factor with phage P1. J. Bacteriol. 88:1266-1276. 1964.-During an investigation of the transduction of R factors with phage P1, a phage lysate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.5.1266-1276.1964

    authors: KONDO E,MITSUHASHI S

    更新日期:1964-11-01 00:00:00

  • Role of the ganSPQAB Operon in Degradation of Galactan by Bacillus subtilis.

    abstract::Bacillus subtilis possesses different enzymes for the utilization of plant cell wall polysaccharides. This includes a gene cluster containing galactan degradation genes (ganA and ganB), two transporter component genes (ganQ and ganP), and the sugar-binding lipoprotein-encoding gene ganS (previously known as cycB). The...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00468-16

    authors: Watzlawick H,Morabbi Heravi K,Altenbuchner J

    更新日期:2016-09-22 00:00:00

  • A Genome-Wide Helicobacter pylori Morphology Screen Uncovers a Membrane-Spanning Helical Cell Shape Complex.

    abstract::Evident in its name, the gastric pathogen Helicobacter pylori has a helical cell morphology which facilitates efficient colonization of the human stomach. An improved light-focusing strategy allowed us to robustly distinguish even subtle perturbations of H. pylori cell morphology by deviations in light-scattering prop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00724-18

    authors: Yang DC,Blair KM,Taylor JA,Petersen TW,Sessler T,Tull CM,Leverich CK,Collar AL,Wyckoff TJ,Biboy J,Vollmer W,Salama NR

    更新日期:2019-06-21 00:00:00

  • Mutations in type I and type IV pilus biosynthetic genes affect twitching motility rates in Xylella fastidiosa.

    abstract::Xylella fastidiosa possesses both type I and type IV pili at the same cell pole. By use of a microfluidic device, the speed of twitching movement by wild-type cells on a glass surface against the flow direction of media was measured as 0.86 (standard error [SE], 0.04) microm min(-1). A type I pilus mutant (fimA) moved...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00934-07

    authors: De La Fuente L,Burr TJ,Hoch HC

    更新日期:2007-10-01 00:00:00

  • Molecular characterization and lytic activities of Streptococcus agalactiae bacteriophages and determination of lysogenic-strain features.

    abstract::The application of mitomycin C induction to 114 genetically diverse Streptococcus agalactiae strains generated 36 phage suspensions. On electron microscopy of the phage suspensions, it was possible to assign the phages to the Siphoviridae family, with three different morphotypes (A, B, and C). Phage genetic diversity ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00426-09

    authors: Domelier AS,van der Mee-Marquet N,Sizaret PY,Héry-Arnaud G,Lartigue MF,Mereghetti L,Quentin R

    更新日期:2009-08-01 00:00:00

  • Effect of cell cycle position on thermotolerance in Saccharomyces cerevisiae.

    abstract::We showed that the heat killing curve for exponentially growing Saccharomyces cerevisiae was biphasic. This suggests two populations of cells with different thermal killing characteristics. When exponentially growing cells separated into cell cycle-specific fractions via centrifugal elutriation were heat shocked, the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.2.779-784.1987

    authors: Plesset J,Ludwig JR,Cox BS,McLaughlin CS

    更新日期:1987-02-01 00:00:00

  • Effect of D-amino acids on structure and synthesis of peptidoglycan in Escherichia coli.

    abstract::Growth of Escherichia coli in the presence of certain D-amino acids, such as D-methionine, results in the incorporation of the D-amino acid into macromolecular peptidoglycan and can be lethal at high concentrations. Previous studies suggested that incorporation was independent of the normal biosynthetic pathway. An en...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.17.5549-5559.1992

    authors: Caparrós M,Pisabarro AG,de Pedro MA

    更新日期:1992-09-01 00:00:00

  • RhaU of Rhizobium leguminosarum is a rhamnose mutarotase.

    abstract::Of the nine genes comprising the L-rhamnose operon of Rhizobium leguminosarum, rhaU has not been assigned a function. The construction of a Delta rhaU strain revealed a growth phenotype that was slower than that of the wild-type strain, although the ultimate cell yields were equivalent. The transport of L-rhamnose int...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01120-07

    authors: Richardson JS,Carpena X,Switala J,Perez-Luque R,Donald LJ,Loewen PC,Oresnik IJ

    更新日期:2008-04-01 00:00:00

  • Corrected Genome Annotations Reveal Gene Loss and Antibiotic Resistance as Drivers in the Fitness Evolution of Salmonella enterica Serovar Typhimurium.

    abstract::Horizontal acquisition of novel chromosomal genes is considered to be a key process in the evolution of bacterial pathogens. However, the identification of gene presence or absence could be hindered by the inconsistencies in bacterial genome annotations. Here, we performed a cross-annotation of omnipresent core and mo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00545-16

    authors: Paul S,Sokurenko EV,Chattopadhyay S

    更新日期:2016-11-04 00:00:00

  • Defective gamma subunit of ATP synthase (F1F0) from Escherichia coli leads to resistance to aminoglycoside antibiotics.

    abstract::A strain of Escherichia coli which was derived from a gentamicin-resistant clinical isolate was found to be cross-resistant to neomycin and streptomycin. The molecular nature of the genetic defect was found to be an insertion of two GC base pairs in the uncG gene of the mutant. The insertion led to the production of a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.3.1435-1444.1989

    authors: Humbert R,Altendorf K

    更新日期:1989-03-01 00:00:00

  • VirE1 protein mediates export of the single-stranded DNA-binding protein VirE2 from Agrobacterium tumefaciens into plant cells.

    abstract::Agrobacterium tumefaciens transfers single-stranded DNAs (T strands) into plant cells. VirE1 and VirE2, which is a single-stranded DNA binding protein, are important for tumorigenesis. We show that T strands and VirE2 can enter plant cells independently and that export of VirE2, but not of T strands, depends on VirE1....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.4.1207-1212.1996

    authors: Sundberg C,Meek L,Carroll K,Das A,Ream W

    更新日期:1996-02-01 00:00:00

  • Repression of nitrate reductase in Neurospora studied by using L-methionine-DL-sulfoximine and glutamine auxotroph gln-1b.

    abstract::The effect of L-methionine-DL-sulfoximine, an inhibitor of glutamine synthetase, on the formation of nitrate reductase in the wild-type strain of Neurospora in the presence of ammonium ions and of glutamine was studied. Under conditions in which glutamine synthetase was inactivated, it was found that only glutamine co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.1.411-415.1980

    authors: Premakumar R,Sorger GJ,Gooden D

    更新日期:1980-07-01 00:00:00

  • Cyclic AMP receptor protein and TyrR are required for acid pH and anaerobic induction of hyaB and aniC in Salmonella typhimurium.

    abstract::Two acid-inducible genes, aniC and aciK, that require anaerobiosis and tyrosine for expression were identified as orf326a encoding a potential amino acid/polyamine antiporter and hyaB encoding hydrogenase I, respectively. Cyclic AMP (cAMP) receptor protein, cAMP, and TyrR, regulator of aromatic amino acid metabolism, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.2.689-694.1999

    authors: Park KR,Giard JC,Eom JH,Bearson S,Foster JW

    更新日期:1999-01-01 00:00:00

  • Molecular cloning of copper resistance genes from Pseudomonas syringae pv. tomato.

    abstract::A cosmid library of copper-resistant (Cur) Pseudomonas syringae pv. tomato PT23 plasmid DNA was constructed and mobilized into the copper-sensitive recipient P. syringae pv. syringae PS61. One resultant cosmid clone, pCOP1 (46 kilobases), conferred copper resistance. The PT23 Cur gene(s) was located on pCOP1 by subclo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.2.470-474.1987

    authors: Bender CL,Cooksey DA

    更新日期:1987-02-01 00:00:00

  • Proposal for classifying human strain navel and related simian mycoplasmas as Mycoplasma primatum sp. n.

    abstract::Mycoplasmas isolated from simian (Cercopithecus aethiops) tissues were shown to have biological, biochemical, and serological properties and electrophoretic cell protein patterns similar to strain Navel isolated from man 15 years ago. The simian and human Navel strains comprised a single serogroup, distinct from the e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.1.439-445.1971

    authors: DelGiudice RA,Carski TR,Barile MF,Lemcke RM,Tully JG

    更新日期:1971-10-01 00:00:00

  • Subunits of succinyl-coenzyme A synthetase: coordination of production in Escherichia coli and discovery of a factor that precludes refolding.

    abstract::Succinyl-coenzyme A synthetase of Escherichia coli has an alpha 2 beta 2 subunit structure. By measuring reconstituted enzyme activity present after addition of purified alpha or beta subunits to cell extracts followed by refolding, we have shown that extracts contain no significant excess of either subunit species. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.1.231-237.1980

    authors: Wolodko WT,Brownie ER,Bridger WA

    更新日期:1980-07-01 00:00:00

  • Cloning and partial sequencing of an operon encoding two Pseudomonas putida haloalkanoate dehalogenases of opposite stereospecificity.

    abstract::We have cloned fragments of DNA (up to 13 kb), from Pseudomonas putida AJ1, that code for two stereospecific haloalkanoate dehalogenases. These enzymes are highly specific for D and L substrates. The two genes, designated hadD and hadL, have been isolated and independently expressed in Escherichia coli and P. putida h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.8.2612-2619.1992

    authors: Barth PT,Bolton L,Thomson JC

    更新日期:1992-04-01 00:00:00

  • Antibiotic production by myxobacteria plays a role in predation.

    abstract::Myxobacteria are predatory and are prolific producers of secondary metabolites. Here, we tested a hypothesized role that secondary metabolite antibiotics function as weapons in predation. To test this, a Myxococcus xanthus Δta1 mutant, blocked in antibiotic TA (myxovirescin) production, was constructed. This TA(-) mut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05052-11

    authors: Xiao Y,Wei X,Ebright R,Wall D

    更新日期:2011-09-01 00:00:00

  • Identification of a novel host-specific IgM protease in Streptococcus suis.

    abstract::Streptococcus suis serotype 2 is a highly invasive, extracellular pathogen in pigs with the capacity to cause severe infections in humans. This study was initiated by the finding that IgM degradation products are released after opsonization of S. suis. The objective of this work was to identify the bacterial factor re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01875-12

    authors: Seele J,Singpiel A,Spoerry C,von Pawel-Rammingen U,Valentin-Weigand P,Baums CG

    更新日期:2013-03-01 00:00:00

  • Synthesis and localization of the Salmonella SPI-1 type III secretion needle complex proteins PrgI and PrgJ.

    abstract::An essential component of type III secretion systems (TTSS) is a supramolecular structure termed the needle complex. In Salmonella enterica, at least four proteins make up this structure: InvG, PrgH, PrgK, and PrgI. Another protein, PrgJ, is thought to play a role in the assembly of this structure, but its function is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.11.3480-3483.2003

    authors: Sukhan A,Kubori T,Galán JE

    更新日期:2003-06-01 00:00:00

  • Genome sequence of Micromonospora lupini Lupac 08, isolated from root nodules of Lupinus angustifolius.

    abstract::Micromonospora strains have been isolated from diverse niches, including soil, water, and marine sediments and root nodules of diverse symbiotic plants. In this work, we report the genome sequence of Micromonospora lupini Lupac 08 isolated from root nodules of the wild legume Lupinus angustifolious. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00628-12

    authors: Alonso-Vega P,Normand P,Bacigalupe R,Pujic P,Lajus A,Vallenet D,Carro L,Coll P,Trujillo ME

    更新日期:2012-08-01 00:00:00

  • Transcriptional regulation in the obligate intracytoplasmic bacterium Rickettsia prowazekii.

    abstract::Transcriptional regulation was demonstrated in Rickettsia prowazekii, an obligate intracytoplasmic bacterium. The level of citrate synthase (gltA) mRNA II, from promoter P2, was greater in the total RNA isolated from heavily infected L929 cells than in moderately infected L929 cells; conversely, the level of ATP/ADP t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.18.5543-5545.1996

    authors: Cai J,Winkler HH

    更新日期:1996-09-01 00:00:00

  • Control of chromosome replication in thymine-requiring strains of Bacillus subtilis 168.

    abstract::Study of the replication pattern of a number of B. subtilis 168 strains under controlled physiological conditions revealed great interstrain variation in control of replication. Replication patterns were calculated from ratios of purA16/leu-8 and purA16/metB5 transformation frequency. The thymine-independent strains a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.3.1055-1067.1975

    authors: Gillin FD,Ganesan AT

    更新日期:1975-09-01 00:00:00

  • An amino acid substitution in penicillin-binding protein 3 creates pointed polar caps in Escherichia coli.

    abstract::The pbpB gene product penicillin-binding protein 3 (PBP3) of Escherichia coli is one of the major targets of beta-lactam antibiotics. At the permissive temperature, the temperature-sensitive pbpBr1 mutant, which was obtained after selection for increased resistance to cephalexin, shows a dramatic change in shape which...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.10.4828-4837.1988

    authors: Taschner PE,Ypenburg N,Spratt BG,Woldringh CL

    更新日期:1988-10-01 00:00:00