Mutations in type I and type IV pilus biosynthetic genes affect twitching motility rates in Xylella fastidiosa.

Abstract:

:Xylella fastidiosa possesses both type I and type IV pili at the same cell pole. By use of a microfluidic device, the speed of twitching movement by wild-type cells on a glass surface against the flow direction of media was measured as 0.86 (standard error [SE], 0.04) microm min(-1). A type I pilus mutant (fimA) moved six times faster (4.85 [SE, 0.27] microm min(-1)) and a pilY1 mutant moved three times slower (0.28 [SE, 0.03] microm min(-1)) than wild-type cells. Type I pili slow the rate of movement, while the putative type IV pilus protein PilY1 is likely important for attachment to surfaces.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

De La Fuente L,Burr TJ,Hoch HC

doi

10.1128/JB.00934-07

subject

Has Abstract

pub_date

2007-10-01 00:00:00

pages

7507-10

issue

20

eissn

0021-9193

issn

1098-5530

pii

JB.00934-07

journal_volume

189

pub_type

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