Abstract:
:Of the nine genes comprising the L-rhamnose operon of Rhizobium leguminosarum, rhaU has not been assigned a function. The construction of a Delta rhaU strain revealed a growth phenotype that was slower than that of the wild-type strain, although the ultimate cell yields were equivalent. The transport of L-rhamnose into the cell and the rate of its phosphorylation were unaffected by the mutation. RhaU exhibits weak sequence similarity to the formerly hypothetical protein YiiL of Escherichia coli that has recently been characterized as an L-rhamnose mutarotase. To characterize RhaU further, a His-tagged variant of the protein was prepared and subjected to mass spectrometry analysis, confirming the subunit size and demonstrating its dimeric structure. After crystallization, the structure was refined to a 1.6-A resolution to reveal a dimer in the asymmetric unit with a very similar structure to that of YiiL. Soaking a RhaU crystal with L-rhamnose resulted in the appearance of beta-L-rhamnose in the active site.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Richardson JS,Carpena X,Switala J,Perez-Luque R,Donald LJ,Loewen PC,Oresnik IJdoi
10.1128/JB.01120-07subject
Has Abstractpub_date
2008-04-01 00:00:00pages
2903-10issue
8eissn
0021-9193issn
1098-5530pii
JB.01120-07journal_volume
190pub_type
杂志文章abstract::L-Glutamate decarboxylase (GAD) activity of Clostridium perfringens (ATCC 8009) cells grown in various culture conditions was investigated. Remarkable variations of GAD level occur during the growth cycle in thioglycollate broth. These changes are affected by the pH of the culture medium. Addition of alkali to the cul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.3.1115-1123.1975
更新日期:1975-09-01 00:00:00
abstract::The RecA proteins of the unusually strong protease-constitutive mutants recA1202 and recA1211 can use RNA in addition to single-stranded DNA (ssDNA) as a cofactor in the cleavage of the LexA repressor in vitro. In the presence of rRNA or tRNA, the effectiveness of these proteins decreased in the order RecA1202 greater...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4823-4827.1988
更新日期:1988-10-01 00:00:00
abstract::Bacterial cell division is mediated by a set of proteins that assemble to form a large multiprotein complex called the divisome. Recent studies in Bacillus subtilis and Escherichia coli indicate that cell division proteins are involved in multiple cooperative binding interactions, thus presenting a technical challenge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00462-08
更新日期:2008-09-01 00:00:00
abstract::The 50S ribosomal subunits obtained from exponential-phase and valine-limited cultures of Streptococcus faecalis 9790 differed in protein content. Approximately 35% of the valine-limited subunits showed protein losses when analyzed by CsCl density centrifugation after formaldehyde fixation. Results of density gradient...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.190-199.1971
更新日期:1971-01-01 00:00:00
abstract::The main chain of teichoic acids can be assembled in cell-free membrane preparations by the transfer of residues from the appropriate nucleotide precursors to an incompletely characterized amphiphilic molecule, lipoteichoic acid carrier (LTC). However, in the cell wall, the main chain is attached to peptidoglycan thro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.3.1222-1231.1981
更新日期:1981-03-01 00:00:00
abstract::Holins are a diverse group of small integral membrane proteins elaborated by bacteriophages to lyse bacterial hosts and effect release of progeny phages in a precisely timed manner. Recently, the holin S gene of phage lambda was overexpressed and the holin protein was purified to homogeneity by means of an oligohistid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.16.4199-4211.1998
更新日期:1998-08-01 00:00:00
abstract::The complete sequences of the RhsB and RhsC elements of Escherichia coli K-12 have been determined. These sequence data reveal a new repeated sequence, called H-rpt (Hinc repeat), which is distinct from the Rhs core repetition that is found in all five Rhs elements. H-rpt is found in RhsB, RhsC, and RhsE. Characteriza...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.10.2799-2808.1993
更新日期:1993-05-01 00:00:00
abstract::The potent tumor-promoting agent croton oil, which has been shown previously to be strongly mitogenic in mammalian cells, stimulates cell division in snake mutants of the blue-green bacterium Agmenellum quadruplicatum. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.2.874-875.1973
更新日期:1973-05-01 00:00:00
abstract::Escherichia coli K-12, polAl(-) is a mutant strain whose extracts are deficient in Kornberg deoxyribonucleic acid (DNA) polymerase activity. We have compared the mutant and parental strains on the basis of a number of responses to ultraviolet (UV) and X-irradiation. For both types of radiation, the mutant is more sens...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.1.61-67.1971
更新日期:1971-07-01 00:00:00
abstract::Coxiella burnetii, the etiological agent of acute and chronic Q fever in humans, is a naturally intracellular pathogen that directs the formation of an acidic Coxiella-containing vacuole (CCV) derived from the host lysosomal network. Central to its pathogenesis is a specialized type IVB secretion system (T4SS) that de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00071-13
更新日期:2013-09-01 00:00:00
abstract::A new pathway for aerobic benzoate oxidation has been postulated for Azoarcus evansii and for a Bacillus stearothermophilus-like strain. Benzoate is first transformed into benzoyl coenzyme A (benzoyl-CoA), which subsequently is oxidized to 3-hydroxyadipyl-CoA and then to 3-ketoadipyl-CoA; all intermediates are CoA thi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.22.6301-6315.2002
更新日期:2002-11-01 00:00:00
abstract::The formation of bacteriolytic enzymes of Staphylococcus aureus, with special reference to strain M18, was investigated under a variety of conditions. The bacteriolytic activity was tested by using whole cells of Micrococcus lysodeikticus as a substrate. Complex media were required for production, and a Casein Hydroly...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.227-233.1970
更新日期:1970-10-01 00:00:00
abstract::The broad-host-range plasmid RK2 is capable of replication and stable maintenance within a wide range of gram-negative bacterial hosts. It encodes the essential replication initiation protein TrfA, which binds to the host initiation protein, DnaA, at the plasmid origin of replication (oriV). There are two versions of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00231-06
更新日期:2006-08-01 00:00:00
abstract::Escherichia coli mutants lacking activities of all known cytosolic ATP-dependent proteases (Lon, ClpAP, ClpXP, and HslVU), due to double deletions [DeltahslVU and Delta(clpPX-lon)], cannot grow at low (30 degrees C) or very high (45 degrees C) temperatures, unlike those carrying either of the deletions. Such growth de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.12.3674-3680.1999
更新日期:1999-06-01 00:00:00
abstract::The production of extracellular enzymes such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt) is activated by the cell density (quorum)-sensing signal, N-(3-oxohexanoyl)-L-homoserine lactone (HSL); plant signals; and aep genes during postexponential growth of Erwinia carotovora subs...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.17.5108-5115.1995
更新日期:1995-09-01 00:00:00
abstract::Several mutants of Bacillus subtilis deficient in catalase synthesis generated by nitrosoguanidine mutagenesis have been used to map a locus affecting catalase activity. Two- and three-factor bacteriophage PBS1 transductional crosses were used to locate the locus, named katA, between recH and thiA with 98% linkage to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5848-5851.1987
更新日期:1987-12-01 00:00:00
abstract::An inorganic pyrophosphatase was purified over 600-fold to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme is a tetramer of Mr = 84,000, has a sedimentation coefficient of 5.8S, a Stokes radius of 3.5 nm, and an isoelectric point of 5.7. Like the enzyme of Escherichia coli, the pyrophosphatase ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.1.318-321.1986
更新日期:1986-10-01 00:00:00
abstract::Listeria monocytogenes is a facultative intracellular gram-positive bacterium responsible for severe opportunistic infections in humans and animals. We had previously identified a gene encoding a putative UDP-N-acetylglucosamine 2-epimerase, a precursor of the teichoic acid linkage unit, in the genome of L monocytogen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00771-06
更新日期:2006-09-01 00:00:00
abstract::In Escherichia coli anaerobic growth lowers the basal or induced levels of numerous enzymes associated with aerobic metabolism. Mutations in arcA (dye) at min 0 relieve this pleiotropic anaerobic repression and render the cell sensitive to the redox dye toluidine blue. In this study we identified a second pleiotropic ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.868-873.1989
更新日期:1989-02-01 00:00:00
abstract::Acinetobacter baumannii is a severe threat to human health as a frequently multidrug-resistant hospital-acquired pathogen. Part of the danger from this bacterium comes from its genome plasticity and ability to evolve quickly by taking up and recombining external DNA into its own genome in a process called natural comp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00034-21
更新日期:2021-01-25 00:00:00
abstract::Escherichia coli outer membrane protein E was purified, and its amino acid composition and N-terminal amino acid were determined. The purified protein was shown to be immunologically and electrophoretically identical to proteins Ic (U. Henning, W. Schmidmayr, and I. Hindennach, Mol. Gen. Genet. 154:293-298, 1977) and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.418-423.1979
更新日期:1979-08-01 00:00:00
abstract::Microtubular structures, which were demonstrated as straight, dense-walled cylinders attached to cell membranes, were found in a stable staphylococcal L-form grown in the absence of antibiotic. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.3.1024-1026.1977
更新日期:1977-12-01 00:00:00
abstract::Erythrobacter litoralis has been known as a bacteriochlorophyll a-containing, aerobic, anoxygenic, phototrophic bacterium. Here we announce the complete genome sequence of E. litoralis HTCC2594, which is devoid of phototrophic potential. E. litoralis HTCC2594, isolated by dilution-to-extinction culturing from seawater...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00026-09
更新日期:2009-04-01 00:00:00
abstract::The gap-2 gene, encoding the NAD(P)-dependent D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH2) of the cyanobacterium Synechocystis sp. strain PCC 6803, was cloned by functional complementation of an Escherichia coli gap mutant with a genomic DNA library; this is the first time that this cloning strategy has been us...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.14.4513-4522.1997
更新日期:1997-07-01 00:00:00
abstract::We have isolated transposon insertions in the lacA gene encoding an endogenous beta-galactosidase of Bacillus subtilis. Upstream of the putative operon containing lacA is a negative regulator, lacR, which encodes a product related to a family of regulators that includes the lactose repressor, lacI, of Escherichia coli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.17.5636-5638.1997
更新日期:1997-09-01 00:00:00
abstract::Escherichia coli cells are shown to be attracted to the l-amino acids alanine, asparagine, aspartate, cysteine, glutamate, glycine, methionine, serine, and threonine, but not to arginine, cystine, glutamine, histidine, isoleucine, leucine, lysine, phenylalanine, tryptophan, tyrosine, or valine. Bacteria grown in a pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.1.315-326.1972
更新日期:1972-10-01 00:00:00
abstract::Staphylococcus lugdunensis is the only coagulase-negative Staphylococcus species with a locus encoding iron-regulated surface determinant (Isd) proteins. In Staphylococcus aureus, the Isd proteins capture heme from hemoglobin and transfer it across the wall to a membrane-bound transporter, which delivers it into the c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01195-12
更新日期:2012-12-01 00:00:00
abstract::Proteus mirabilis swarming behavior is characterized by the development of concentric rings of growth that are formed as cyclic events of swarmer cell differentiation, swarming migration, and cellular differentiation are repeated during colony translocation across a surface. This cycle produces the bull's-eye colony o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::Crystalline inclusions occur in bacterium 22M. Arrays consist of parallel rows of polyhedral subunits (7.5 nm in diameter), 2.0 nm apart, with a distance of 3.0 nm between rows. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.3.1390-1394.1969
更新日期:1969-06-01 00:00:00
abstract::Chlamydia spp. express a functional type III secretion system (T3SS) necessary for pathogenesis and intracellular growth. However, certain essential components of the secretion apparatus have diverged to such a degree as to preclude their identification by standard homology searches of primary protein sequences. One e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01671-07
更新日期:2008-03-01 00:00:00