Subunits of succinyl-coenzyme A synthetase: coordination of production in Escherichia coli and discovery of a factor that precludes refolding.

abstract：:In Escherichia coli, putrescine is metabolized to succinate for use as a carbon and nitrogen source by the putrescine utilization pathway (Puu pathway). One gene in the puu gene cluster encodes a transcription factor, PuuR, which has a helix-turn-helix DNA-binding motif. DNA microarray analysis of an E. coli puuR muta...

journal_title：Journal of bacteriology

authors： Nemoto N,Kurihara S,Kitahara Y,Asada K,Kato K,Suzuki H

更新日期：2012-07-01 00:00:00

abstract：:One step in de novo pyridoxine (vitamin B6) and pyridoxal 5'-phosphate biosynthesis was predicted to be an oxidation catalyzed by an unidentified D-erythrose-4-phosphate dehydrogenase (E4PDH). To help identify this E4PDH, we purified the Escherichia coli K-12 gapA- and gapB-encoded dehydrogenases to homogeneity and te...

journal_title：Journal of bacteriology

authors： Zhao G,Pease AJ,Bharani N,Winkler ME

更新日期：1995-05-01 00:00:00

abstract：:A study was made of how the catabolism of a carbon and energy source is affected by the biosynthetic demands of growing bacterial cells. Cultures of Aerobacter aerogenes in l-histidine medium were grown in a chemostat at rates determined by the supply of either sulfate or a required amino acid, l-arginine. It was disc...

journal_title：Journal of bacteriology

authors： Jensen DE,Neidhardt FC

更新日期：1969-04-01 00:00:00

abstract：:The surface protein composition of members of a serogroup of Aeromonas hydrophila which exhibit high virulence for fish was examined. Treatment of whole cells of representative strain A. hydrophila TF7 with 0.2 M glycine buffer (pH 4.0) resulted in the release of sheets of a tetragonal surface protein array. Sodium do...

journal_title：Journal of bacteriology

authors： Dooley JS,Trust TJ

更新日期：1988-02-01 00:00:00

abstract：:The F-plasmid-encoded TraI protein, also known as DNA helicase I, is a bifunctional protein required for conjugative DNA transfer. The enzyme catalyzes two distinct but functionally related reactions required for the DNA processing events associated with conjugation: the site- and strand-specific transesterification (...

journal_title：Journal of bacteriology

authors： Matson SW,Ragonese H

更新日期：2005-01-01 00:00:00

abstract：:The Czc system of Alcaligenes eutrophus mediates resistance to cobalt, zinc, and cadmium through ion efflux catalyzed by the CzcCB2A cation-proton antiporter. DNA sequencing of the region upstream of the czcNICBADRS determinant located on megaplasmid pMOL30 revealed the 5' end of czcN and a gene for a MgtC-like protei...

journal_title：Journal of bacteriology

authors： Grosse C,Grass G,Anton A,Franke S,Santos AN,Lawley B,Brown NL,Nies DH

更新日期：1999-04-01 00:00:00

abstract：:A previously constructed trp-S-lacZ fusion encoding a hybrid protein with beta-galactosidase activity was subcloned from a multicopy plasmid onto a lambda vector. Single-copy lysogens of lambda trpS-lacZ were used to determine whether trpS was regulated in a manner similar to that of other aminoacyl-tRNA synthetases. ...

journal_title：Journal of bacteriology

authors： Hall CV,Yanofsky C

更新日期：1982-08-01 00:00:00

abstract：:Three homofermentative (Lactobacillus plantarum B38, L. plantarum B33, Pediococcus pentosaceus B30) and three heterofermentative (Leuconostoc mesenteroides 39, L. oenos B70, Lactobacillus brevis) lactic acid bacteria were examined for the presence or absence of nicotinamide adenine dinucleotide (NAD)-dependent and NAD...

journal_title：Journal of bacteriology

authors： Doelle HW

更新日期：1971-12-01 00:00:00

abstract：:Isolated cell envelopes of a marine bacterium, M.B.3, have been prepared which possess a nonspecific, cation-activated nucleotidase. The cell envelope comprises approximately 35% (dry weight) of the whole cell and contains protein, 60.2%; lipids, 20.7%; hexose, 3.4%; and ribonucleic acid, 4.6%. No deoxyribonucleic aci...

journal_title：Journal of bacteriology

authors： Thompson J,Green ML,Happold FC

更新日期：1969-09-01 00:00:00

abstract：:Rhodobacter sphaeroides cells containing an in-frame deletion within ccmA lack detectable soluble and membrane-bound c-type cytochromes and are unable to grow under conditions where these proteins are required. Only strains merodiploid for ccmABCDG were found after attempting to generate cells containing either a ccmG...

journal_title：Journal of bacteriology

authors： Cox RL,Patterson C,Donohue TJ

更新日期：2001-08-01 00:00:00

abstract：:The extracellular protease of Pseudomonas maltophilia was partially purified by ammonium sulfate precipitation and chromatography on Sephadex G-75 and Bio-rex 70. Gel electrophoresis revealed minor impurities. The enzyme exhibited the following properties: (i) molecular weight, 35,000; (ii) A see article; 10.8; (iii) ...

journal_title：Journal of bacteriology

authors： Boethling RS

更新日期：1975-03-01 00:00:00

abstract：:We used two-dimensional gel electrophoresis to quantitate the changes in rates of synthesis that follow phage lambda infection for 21 Escherichia coli proteins, including groE and dnaK proteins. Although total protein synthesis and the rates of synthesis of most individual E. coli proteins decreased after infection, s...

journal_title：Journal of bacteriology

authors： Drahos DJ,Hendrix RW

更新日期：1982-03-01 00:00:00

abstract：:Pigmentation mutants of Pseudomonas aeruginosa, selected by observed visual differences in coloration from the wild-type strain, were examined for altered patterns of phenazine synthesis. Three classes of mutants that were incapable of pyocyanine production were identified. Pigmentation patterns that were found to cha...

journal_title：Journal of bacteriology

authors： Byng GS,Eustice DC,Jensen RA

更新日期：1979-06-01 00:00:00

abstract：:In response to iron limitation, the siderophore enterobactin is synthesized and secreted by Escherichia coli. Its biosynthesis is performed by a series of enzymes encoded by the Ent gene cluster. Among the genes of this cluster, ybdB has not been implicated in enterobactin production to date. We demonstrate here an in...

journal_title：Journal of bacteriology

authors： Leduc D,Battesti A,Bouveret E

更新日期：2007-10-01 00:00:00

abstract：:A bacterium was isolated by elective culture with p-hydroxybenzoate as substrate and nitrate as electron acceptor. It grew either aerobically or anaerobically, by nitrate respiration, on a range of aromatic compounds. The organism was identified as a pseudomonad and was given the trivial name Pseudomonas PN-1. Benzoat...

journal_title：Journal of bacteriology

authors： Taylor BF,Campbell WL,Chinoy I

更新日期：1970-05-01 00:00:00

abstract：:Borrelia burgdorferi, the Lyme disease agent, and other members of the spirochetal genus Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids. The copy number relative to the chromosome was determined for 49- and 16-kb linear plasmids and a 27-kb circular plasmid of the type strai...

journal_title：Journal of bacteriology

authors： Hinnebusch J,Barbour AG

更新日期：1992-08-01 00:00:00

abstract：:The rate at which the peptidoglycan precursor meso-diaminopimelic acid (DAP) is incorporated into the cell wall of Escherichia coli cells was determined by pulse-label experiments. For different E. coli strains, the incorporation rate was compared with the rate of uptake of DAP into the cell. With E. coli W7, a dap ly...

journal_title：Journal of bacteriology

authors： Wientjes FB,Pas E,Taschner PE,Woldringh CL

更新日期：1985-10-01 00:00:00

abstract：:Glycerol trinitrate (GTN) reductase, which enables Agrobacterium radiobacter to utilize GTN and related explosives as sources of nitrogen for growth, was purified and characterized, and its gene was cloned and sequenced. The enzyme was a 39-kDa monomeric protein which catalyzed the NADH-dependent reductive scission of...

journal_title：Journal of bacteriology

authors： Snape JR,Walkley NA,Morby AP,Nicklin S,White GF

更新日期：1997-12-01 00:00:00

abstract：:Resting cells of Corynebacterium nephridii reduce nitrate, nitrite, and nitric oxide to nitrous oxide under anaerobic conditions. Nitrous oxide production from nitrite was optimal from pH 7.0 to 7.4. The stoichiometry of nitrous oxide production from nitrite was 99% of the theoretical-two moles of nitrite was used for...

journal_title：Journal of bacteriology

authors： Renner ED,Becker GE

更新日期：1970-03-01 00:00:00

abstract：:Siderophore-mediated iron transport in Pseudomonas aeruginosa is dependent upon the cytoplasmic membrane-associated TonB1 energy coupling protein for activity. To assess the functional significance of the various regions of this molecule and to identify functionally important residues, the tonB1 gene was subjected to ...

journal_title：Journal of bacteriology

authors： Zhao Q,Poole K

更新日期：2002-03-01 00:00:00

abstract：:Twelve mutants of Escherichia coli K-12 have been isolated which carry chromosomal mutations that exhibit pleiotropic effects on the expression of F factor tra cistrons. F pilus synthesis, deoxyribonucleic acid transfer, and surface exclusion are all inhibited. Six of the mutants carry sfrA mutations, and six carry sf...

journal_title：Journal of bacteriology

authors： Beutin L,Achtman M

更新日期：1979-09-01 00:00:00

abstract：:Brubaker, Robert R. (Fort Detrick, Frederick, Md.) and Michael J. Surgalla. Pesticins. II. Production of pesticin I and II. J. Bacteriol. 84:539-545. 1962.-Pesticin I was separated from pesticin I inhibitor by ion-exchange chromatography of cell-free culture supernatant fluids and by acid precipitation of soluble prep...

journal_title：Journal of bacteriology

authors： BRUBAKER RR,SURGALLA MJ

更新日期：1962-09-01 00:00:00

abstract：:MurG is a peripheral membrane protein that is one of the key enzymes in peptidoglycan biosynthesis. The crystal structure of Escherichia coli MurG (S. Ha, D. Walker, Y. Shi, and S. Walker, Protein Sci. 9:1045-1052, 2000) contains a hydrophobic patch surrounded by basic residues that may represent a membrane associatio...

journal_title：Journal of bacteriology

authors： van den Brink-van der Laan E,Boots JW,Spelbrink RE,Kool GM,Breukink E,Killian JA,de Kruijff B

更新日期：2003-07-01 00:00:00

abstract：:In the nosocomial opportunistic pathogen Acinetobacter baumannii, RecA-dependent mutagenesis, which causes antibiotic resistance acquisition, is linked to the DNA damage response (DDR). Notably, unlike the Escherichia coli paradigm, recA and DDR gene expression in A. baumannii is bimodal. Namely, there is phenotypic v...

journal_title：Journal of bacteriology

authors： Ching C,Gozzi K,Heinemann B,Chai Y,Godoy VG

更新日期：2017-05-09 00:00:00

abstract：:Short-term labeling with radioactive thymidine led to inaccurate estimates of deoxyribonucleic acid synthesis at some growth rates in steady-state cultures of Escherichia coli B/r. Estimates were corrected in chemostat cultures by adding adenosine, a known inhibitor of thymidine phosphorylase. ...

journal_title：Journal of bacteriology

authors： Kubitschek HE,Valdez D,Freedman ML

更新日期：1972-06-01 00:00:00

abstract：:A study was made to determine whether factors other than the availability of phosphorus were involved in the regulation of synthesis of teichoic and teichuronic acids in Bacillus subtilis subsp. niger WM. First, the nature of the carbon source was varied while the dilution rate was maintained at about 0.3 h-1. Irrespe...

journal_title：Journal of bacteriology

authors： Kruyssen FJ,de Boer WR,Wouters JT

更新日期：1980-10-01 00:00:00

abstract：:Salmonella typhimurium was found to utilize 2-deoxyribose as a sole carbon and energy source. Cells grown in the presence of deoxyribose contained increased levels of deoxyribose kinase, thymidine phosphorylase, and two forms of deoxyribose-5-phosphate aldolase (DR5P aldolase). One form of DR5P aldolase was induced by...

journal_title：Journal of bacteriology

authors： Hoffee PA

更新日期：1968-02-01 00:00:00

abstract：:TolB is a periplasmic protein of the cell envelope Tol complex. It is partially membrane associated through an interaction with the outer membrane lipoprotein PAL (peptidoglycan-associated lipoprotein), which also belongs to the Tol system. The interaction of TolB with outer membrane porins of Escherichia coli was inv...

journal_title：Journal of bacteriology

authors： Rigal A,Bouveret E,Lloubes R,Lazdunski C,Benedetti H

更新日期：1997-12-01 00:00:00

abstract：:Motility of bacteria like Salmonella enterica is a highly regulated process that responds to a variety of internal and external stimuli. A hierarchy of three promoter classes characterizes the Salmonella flagellar system, and the onset of flagellar gene expression depends on the oligomeric regulatory complex and class...

journal_title：Journal of bacteriology

authors： Singer HM,Erhardt M,Hughes KT

更新日期：2013-09-01 00:00:00

abstract：:In Acinetobacter calcoaceticus the seven genes coding for the enzymes responsible for tryptophan synthesis map at three chromosomal locations. Two three-gene clusters, one (trpGDC) specifying the small subunit of anthranilate synthase, phosphoribosyl transferase, and indoleglycerol phosphate synthase and the other (tr...

journal_title：Journal of bacteriology

authors： Cohn W,Crawford IP

更新日期：1976-07-01 00:00:00