The hotdog thioesterase EntH (YbdB) plays a role in vivo in optimal enterobactin biosynthesis by interacting with the ArCP domain of EntB.

Abstract:

:In response to iron limitation, the siderophore enterobactin is synthesized and secreted by Escherichia coli. Its biosynthesis is performed by a series of enzymes encoded by the Ent gene cluster. Among the genes of this cluster, ybdB has not been implicated in enterobactin production to date. We demonstrate here an in vivo role for the hotdog protein EntH (YbdB) in the optimal production of enterobactin. Indeed, we showed that EntH is a thioesterase specifically produced under iron limitation conditions. Furthermore, EntH interacts specifically with the aryl carrier protein (ArCP) domain of EntB, a crucial bifunctional enzyme of the enterobactin biosynthesis pathway and a potential target of EntH thioesterase activity. A strain devoid of EntH is impaired for growth under iron limitation associated with the presence of the salicylate inhibitor, correlating with the diminution of enterobactin production under these conditions. Normal growth and enterobactin production are restored upon expression of entH in trans. Inversely, unnecessary overproduction of EntH provokes a fall of the quantity of siderophore produced under iron starvation conditions. Our findings point to a proofreading role for EntH during biosynthesis of enterobactin in vivo. EntH thioesterase activity could be required for cleaving wrongly charged molecules on the carrier protein EntB. This is the first description of such a role in the optimization of a nonribosomal biosynthesis pathway for a protein of the hotdog superfamily.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Leduc D,Battesti A,Bouveret E

doi

10.1128/JB.00755-07

subject

Has Abstract

pub_date

2007-10-01 00:00:00

pages

7112-26

issue

19

eissn

0021-9193

issn

1098-5530

pii

JB.00755-07

journal_volume

189

pub_type

杂志文章
  • Crl binds to domain 2 of σ(S) and confers a competitive advantage on a natural rpoS mutant of Salmonella enterica serovar Typhi.

    abstract::The RpoS sigma factor (σ(S)) is the master regulator of the bacterial response to a variety of stresses. Mutants in rpoS arise in bacterial populations in the absence of stress, probably as a consequence of a subtle balance between self-preservation and nutritional competence. We characterized here one natural rpoS mu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00801-10

    authors: Monteil V,Kolb A,Mayer C,Hoos S,England P,Norel F

    更新日期:2010-12-01 00:00:00

  • Secondary and tertiary responses of the induced bactericidin from the West Indian spiny lobster, Panulirus argus.

    abstract::West Indian spiny lobsters, Panulirus argus, synthesized a hemolymph bactericidin after being injected with killed suspensions of gram-negative bacillus EMB-1 isolated from the normal gut of this lobster. To study differences between the primary response and secondary response, animals were given a primary antigen inj...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.3.943-946.1969

    authors: Evans EE,Weinheimer PF,Painter B,Acton RT,Evans ML

    更新日期:1969-06-01 00:00:00

  • Genome sequence of Reyranella massiliensis, a bacterium associated with amoebae.

    abstract::Reyranella massiliensis is an Alphaproteobacterium member of the class Rhodospirillaceae, growing in amoebae. We sequenced the genome of type strain 521(T). It is composed of a 5,792,218-bp chromosome and encodes 5,675 protein-coding genes and 53 RNA genes, including 3 rRNA genes. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01260-12

    authors: Pagnier I,Croce O,Robert C,Raoult D,La Scola B

    更新日期:2012-10-01 00:00:00

  • Response and specificity of antibodies for Shigella flexneri: demonstration of type-specific factors in immunoglobulin G fraction of antiserum.

    abstract::Considerable amounts of immunoglobulin G (IgG) antibody appeared in hyperimmune rabbit serum at a late period during a course of immunization with several injections of Shigella flexneri O antigens. High yields of IgG antibody possessing homogenous specificity could be fractionated from crude gamma-globulin solution o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.3.1128-1134.1969

    authors: Fukazawa Y,Shinoda T,Tsuchiya T

    更新日期:1969-06-01 00:00:00

  • Characterization of the Escherichia coli AaeAB efflux pump: a metabolic relief valve?

    abstract::Treatment of Escherichia coli with p-hydroxybenzoic acid (pHBA) resulted in upregulation of yhcP, encoding a protein of the putative efflux protein family. Also upregulated were the adjacent genes yhcQ, encoding a protein of the membrane fusion protein family, and yhcR, encoding a small protein without a known or sugg...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.21.7196-7204.2004

    authors: Van Dyk TK,Templeton LJ,Cantera KA,Sharpe PL,Sariaslani FS

    更新日期:2004-11-01 00:00:00

  • Relevance of DNA alkylation damage repair systems in Salmonella enterica virulence.

    abstract::Systematic inactivation of pathways involved in DNA alkylation damage repair demonstrated that inactivation of the ada, ogt, tag, uvrA, and mfd genes is required to detect a Salmonella enterica virulence decrease. Furthermore, the fitness of S. enterica, defective in these genes, is lowered only when the bacterium is ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01693-09

    authors: Alvarez G,Campoy S,Spricigo DA,Teixidó L,Cortés P,Barbé J

    更新日期:2010-04-01 00:00:00

  • Heat inactivation of Bacillus subtilis spores lacking small, acid-soluble spore proteins is accompanied by generation of abasic sites in spore DNA.

    abstract::Previous work has shown that lethal heat treatment of Bacillus subtilis spores lacking the major DNA-binding proteins SASP-alpha and -beta (alpha-beta- spores) causes significant DNA damage, including many single-strand breaks. In this work we have used a reagent specific for aldehydes present in abasic sites in DNA t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.7.2111-2113.1994

    authors: Setlow B,Setlow P

    更新日期:1994-04-01 00:00:00

  • Genetic evidence of distinct physiological regulation mechanisms in the sigma(54) Pu promoter of Pseudomonas putida.

    abstract::The activity of the toluene-responsive sigma(54) Pu promoter of the pWW0 TOL plasmid of Pseudomonas putida is down-regulated in vivo during exponential growth in rich medium and also by the presence of glucose in the culture. Although the Pu promoter already performs poorly during log growth in minimal medium when ame...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.4.956-960.2000

    authors: Cases I,de Lorenzo V

    更新日期:2000-02-01 00:00:00

  • Control of the kilA gene of the broad-host-range plasmid RK2: involvement of korA, korB, and a new gene, korE.

    abstract::Broad-host-range plasmid RK2 encodes several different kil genes which are potentially lethal to an Escherichia coli host. The kil genes and the essential RK2 replication gene trfA are regulated by the products of kor genes. We have shown previously that kilA can be controlled by a constitutively expressed korA gene. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.3.1315-1320.1987

    authors: Young C,Burlage RS,Figurski DH

    更新日期:1987-03-01 00:00:00

  • Impact of either elevated or decreased levels of cytochrome bd expression on Shigella flexneri virulence.

    abstract::Shigella spp. are the major cause of bacillary dysentery worldwide. The pathogenic process involves bacterial invasion and lysis of the phagocytic vacuole, followed by replication and movement within the cell cytoplasm and, ultimately, spread directly into adjacent cells. This study demonstrates that S. flexneri cytoc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.4.1229-1237.1999

    authors: Way SS,Sallustio S,Magliozzo RS,Goldberg MB

    更新日期:1999-02-01 00:00:00

  • Molecular and expression analysis of the Rhizobium meliloti phosphoenolpyruvate carboxykinase (pckA) gene.

    abstract::The pckA gene of Rhizobium meliloti, encoding phosphoenolpyruvate carboxykinase, was isolated from a genomic cosmid library by complementation of the succinate growth phenotype of a Pck- mutant. The gene region was mapped by subcloning and Tn5 insertion mutagenesis. The DNA sequence for a 2-kb region containing the st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.6.1452-1460.1995

    authors: Osterås M,Driscoll BT,Finan TM

    更新日期:1995-03-01 00:00:00

  • Anaerobic control of denitrification in Pseudomonas stutzeri escapes mutagenesis of an fnr-like gene.

    abstract::The synthesis of proteins necessary for the respiratory reduction of nitrate to dinitrogen is induced in most denitrifying bacteria by a shift to anaerobiosis. A homolog of the fur gene, which encodes a redox-active transcriptional activator in Escherichia coli, was isolated from Pseudomonas stutzeri by using the anr ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.22.7236-7246.1993

    authors: Cuypers H,Zumft WG

    更新日期:1993-11-01 00:00:00

  • Mechanism of acetate synthesis from CO2 by Clostridium acidiurici.

    abstract::Total synthesis of acetate from CO2 by Clostridium acidiurici during fermentations of hypoxanthine has been shown to involve synthesis of glycine from methylenetetrahydrofolate, CO2, and NH3. The glycine is converted to serine by the addition of methylenetetrahydrofolate, and the resulting serine is converted to pyruv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.2.468-478.1979

    authors: Waber LJ,Wood HG

    更新日期:1979-11-01 00:00:00

  • The acrAB homolog of Haemophilus influenzae codes for a functional multidrug efflux pump.

    abstract::Disruption of gene HI0894 or HI0895 in Haemophilus influenzae Rd, homologs of Escherichia coli acrAB multidrug efflux genes, caused hypersusceptibility to erythromycin, rifampin, novobiocin, and dyes such as ethidium bromide and crystal violet and increased accumulation of radioactive erythromycin, showing that these ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.21.6855-6857.1997

    authors: Sánchez L,Pan W,Viñas M,Nikaido H

    更新日期:1997-11-01 00:00:00

  • Arrangement of glycan chains in the sacculus of Escherichia coli.

    abstract::A novel of Escherichia coli endopeptidase was used for a selective partial hydrolysis of the peptide bridges which interlink the glycan chains in E. coli sacculi. The loosening of the murein network revealed, in the electron microscope, a preferential orientation of the glycan chains, more or less perpendicular to the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.2.723-729.1978

    authors: Verwer RW,Nanninga N,Keck W,Schwarz U

    更新日期:1978-11-01 00:00:00

  • Purification of mycobacterial deoxyribonucleic acid.

    abstract::Impurities believed to be polysaccharides have been found in mycobacterial deoxyribonucleic acid (DNA) preparations. Agar-gel diffusion of the DNA preparations against concanavalin A indicated the presence of three polysaccharides and was used to follow the purification procedures. The polysaccharides appeared to be t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.3.1033-1039.1972

    authors: Hill EB,Wayne LG,Gross WM

    更新日期:1972-12-01 00:00:00

  • Regulatory mutants and control of cysteine biosynthetic enzymes in Salmonella typhimurium.

    abstract::The cysB region in Salmonella typhimurium regulates in a positive manner the noncontiguous structural genes for the enzymes responsible for sulfate reduction in cysteine biosynthesis. We treated three cysB mutants with chemical mutagens and selected 81 secondary mutants in which the inability to utilize sulfate was su...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.1.94-101.1976

    authors: Borum PR,Monty KJ

    更新日期:1976-01-01 00:00:00

  • Ultraviolet-sensitive mutator strain of Escherichia coli K-12.

    abstract::An ultraviolet (UV)-sensitive mutator gene, mutU, was identified in Escherichia coli K-12. The mutation mutU4 is very close to uvrD, between metE and ilv, on the E. coli chromosome. It was recessive as a mutator and as a UV-sensitive mutation. The frequency of reversion of trpA46 on an F episome was increased by mutU4...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.1.145-160.1973

    authors: Siegel EC

    更新日期:1973-01-01 00:00:00

  • Lysine catabolism in Streptomyces spp. is primarily through cadaverine: beta-lactam producers also make alpha-aminoadipate.

    abstract::Genetic and biochemical evidence was obtained for lysine catabolism via cadaverine and delta-aminovalerate in both the beta-lactam producer Streptomyces clavuligerus and the nonproducer Streptomyces lividans. This pathway is used when lysine is supplied as the sole source of nitrogen for the organism. A second pathway...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.299-302.1989

    authors: Madduri K,Stuttard C,Vining LC

    更新日期:1989-01-01 00:00:00

  • Effect of environmental pH on morphological development of Candida albicans is mediated via the PacC-related transcription factor encoded by PRR2.

    abstract::The ability to respond to ambient pH is critical to the growth and virulence of the fungal pathogen Candida albicans. This response entails the differential expression of several genes affecting morphogenesis. To investigate the mechanism of pH-dependent gene expression, the C. albicans homolog of pacC, designated PRR...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.24.7524-7530.1999

    authors: Ramon AM,Porta A,Fonzi WA

    更新日期:1999-12-01 00:00:00

  • Spectra of spontaneous growth-dependent and adaptive mutations at ebgR.

    abstract::A comparison of the spectra of spontaneous growth-dependent and adaptive mutations in ebgR shows that both spectra are dominated by insertion sequence (IS)-mediated mutations. The difference between growth-dependent mutations (61% IS mediated) and adaptive mutations (80% IS mediated) is highly significant (P < 0.0001)...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.4.1149-1155.1999

    authors: Hall BG

    更新日期:1999-02-01 00:00:00

  • Streptococcus faecium mutants that are temperature sensitive for cell growth and show alterations in penicillin-binding proteins.

    abstract::The penicillin-binding proteins (PBPs) of 209 cell division (or growth) temperature-sensitive mutants of Streptococcus faecium were analyzed in this study. A total of nine strains showed either constitutive or temperature-sensitive conditional damage in the PBPs. Analysis of these nine strains yielded the following re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.6.2432-2439.1987

    authors: Canepari P,Lleò MM,Fontana R,Satta G

    更新日期:1987-06-01 00:00:00

  • Products of phospholipid metabolism in Bacillus stearothermophilus.

    abstract::The products of phospholipid turnover in Bacillus stearothermophilus were determined in cultures labeled to equilibrium and with short pulses of [32P]phosphate and [2-3H]glycerol. Label lost from the cellular lipid pool was recovered in three fractions: low-molecular-weight extracellular products, extracellular lipid,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.1.294-303.1983

    authors: Card GL,Finn DJ

    更新日期:1983-04-01 00:00:00

  • Tetrapyrrole biosynthesis in Rhodobacter capsulatus is transcriptionally regulated by the heme-binding regulatory protein, HbrL.

    abstract::We demonstrate that the expression of hem genes in Rhodobacter capsulatus is transcriptionally repressed in response to the exogenous addition of heme. A high-copy suppressor screen for regulators of hem gene expression resulted in the identification of an LysR-type transcriptional regulator, called HbrL, that regulat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.4.1567-1576.2006

    authors: Smart JL,Bauer CE

    更新日期:2006-02-01 00:00:00

  • Identification of accessory genome regions in poultry Clostridium perfringens isolates carrying the netB plasmid.

    abstract::Necrotic enteritis (NE) is an economically important disease of poultry caused by certain Clostridium perfringens type A strains. NE pathogenesis involves the NetB toxin, which is encoded on a large conjugative plasmid within a 42-kb pathogenicity locus. Recent multilocus sequence type (MLST) studies have identified t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01032-12

    authors: Lepp D,Gong J,Songer JG,Boerlin P,Parreira VR,Prescott JF

    更新日期:2013-03-01 00:00:00

  • Time course of purine nucleoside phosphorylase occurrence in sporulation of Bacillus cereus.

    abstract::Purine nucleoside phosphorylase (EC 2.4.2.1) from Bacillus cereus T was examined at hourly intervals during growth and sporulation. The enzyme has maximal activity in extracts prepared from cells during stages I and II. The activity during exponential growth is only 6.6% of the maximum and that in free spores is only ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.111.1.33-36.1972

    authors: Englebrecht HL

    更新日期:1972-07-01 00:00:00

  • The Clostridium difficile cpr locus is regulated by a noncontiguous two-component system in response to type A and B lantibiotics.

    abstract::The intestinal pathogen Clostridium difficile is known to grow only within the intestines of mammals, yet little is known about how the bacterium subsists in this environment. In the intestine, C. difficile must contend with innate defenses within the host, such as cationic antimicrobial peptides (CAMPs) produced by t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00166-13

    authors: Suárez JM,Edwards AN,McBride SM

    更新日期:2013-06-01 00:00:00

  • cis-acting sites in the transcript of the Bacillus subtilis trp operon regulate expression of the operon.

    abstract::Transcription of the trp operon of Bacillus subtilis is regulated by attenuation. A trpE'-'lacZ gene fusion preceded by the wild-type trp promoter-leader region was used to analyze regulation. Overproduction of the trp leader transcript in trans from a multicopy plasmid caused constitutive expression of the chromosoma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.3080-3088.1988

    authors: Kuroda MI,Henner D,Yanofsky C

    更新日期:1988-07-01 00:00:00

  • Macromolecule synthesis in yeast spheroplasts.

    abstract::Conditions have been established for the preparation of spheroplasts of Saccharomyces cerevisiae which are able to increase their net content of protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), several-fold upon incubation in a medium stabilized with 1 m sorbitol. The rate of RNA and protein synthesis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.5.1697-1705.1967

    authors: Hutchison HT,Hartwell LH

    更新日期:1967-11-01 00:00:00

  • Effect of nutrient concentration on the growth of Escherichia coli.

    abstract::The relationship between specific growth rate of Escherichia coli and the concentration of limiting nutrient (glucose or phosphate or tryptophan) has been determined for populations in a steady state. At high concentrations the specific growth rate is independent of the concentration of nutrient, but at low concentrat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.107.1.210-216.1971

    authors: Shehata TE,Marr AG

    更新日期:1971-07-01 00:00:00