Pseudomonas stutzeri and related species undergo natural transformation.

Abstract:

:Cells of Pseudomonas stutzeri are naturally transformed by homologous chromosomal DNA; they do not require chemical treatment to become competent. This capacity to undergo natural transformation was found to be shared by the closely related species P. mendocina, P. alcaligenes, and P. pseudoalcaligenes, but was not detectable in strains of P. aeruginosa, P. perfectomarinus, P. putida, P. fluorescens, or P. syringae. P. stutzeri could be transformed either on plates or in liquid medium. Only double-stranded chromosomal DNA was effective; single-stranded DNA and plasmid DNA were not. DNA fragments larger than 10 kilobase pairs were more effective than smaller fragments. The transformation frequency was proportional to DNA concentration from 1 ng/ml to 1 microgram/ml; higher concentrations were saturating. The maximum frequency, about 10(-4) transformants per recipient cell, was obtained with cells from a culture in the early stationary growth phase. A variety of chromosomal mutations have been transformed, including mutations to auxotrophy and to antibiotic resistance. Other systems for genetic exchange in P. stutzeri have not yet been found; transformation offers a means for the genetic analysis of this metabolically versatile organism.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Carlson CA,Pierson LS,Rosen JJ,Ingraham JL

doi

10.1128/JB.153.1.93-99.1983

subject

Has Abstract

pub_date

1983-01-01 00:00:00

pages

93-9

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

153

pub_type

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