Elucidation of an alternate isoleucine biosynthesis pathway in Geobacter sulfurreducens.

abstract：:An in vitro constructed plasmid, pVH15, consisting of the entire genome of the plasmid ColE1, the tryptophan operon of Escherichia coli, and regions of the bacteriophage PHI80pt190, spontaneously gave rise in E. coli to a mini-ColE1 plasmid consisting of approximately one-half of the ColE1 genome and a small segment o...

journal_title：Journal of bacteriology

authors： Hershfield V,Boyer HW,Chow L,Helinski DR

更新日期：1976-04-01 00:00:00

abstract：:In the thermophilic archaeon Methanobacterium thermoautotrophicum Marburg, the structural gene for isoleucyl-tRNA synthetase (ileS) is flanked upstream by orf401 and downstream by purL. orf401 encodes a 43.5-kDa protein with an unknown function. Northern (RNA) hybridization and S1 nuclease protection experiments showe...

journal_title：Journal of bacteriology

authors： Jenal U,Thurner C,Leisinger T

更新日期：1993-09-01 00:00:00

abstract：:A beta-1,4-xylan hydrolase (xylanase A) produced by Erwinia chrysanthemi D1 isolated from corn was analyzed with respect to its secondary structure and enzymatic function. The pH and temperature optima for the enzyme were found to be pH 6.0 and 35 degrees C, with a secondary structure under those conditions that consi...

journal_title：Journal of bacteriology

authors： Hurlbert JC,Preston JF 3rd

更新日期：2001-03-01 00:00:00

abstract：:The nif promoters of Klebsiella pneumoniae must be activated by proteins bound to upstream sequences which are thought to interact with the sigma54-RNA polymerase holoenzyme by DNA looping. NifA is the activator for most of the promoters, and integration host factor (IHF) mediates the DNA looping. While NtrC is the ac...

journal_title：Journal of bacteriology

authors： Cheema AK,Choudhury NR,Das HK

更新日期：1999-09-01 00:00:00

abstract：:The plasmid-encoded epidermin biosynthetic gene epiC of Staphylococcus epidermidis Tü3298 was expressed in Escherichia coli by using the T7 RNA polymerase-promoter system, and the gene product EpiC was identified by Western blotting (immunoblotting) with an anti-EpiC-peptide antiserum. EpiC was a hydrophobic but solub...

journal_title：Journal of bacteriology

authors： Kupke T,Gotz F

更新日期：1996-03-01 00:00:00

abstract：:The widely conserved second messenger cyclic diguanosine monophosphate (c-di-GMP) plays a key role in quorum-sensing (QS)-dependent production of virulence factors in Xanthomonas campestris pv. campestris. The detection of QS diffusible signal factor (DSF) by the sensor RpfC leads to the activation of response regulat...

journal_title：Journal of bacteriology

authors： Tao F,He YW,Wu DH,Swarup S,Zhang LH

更新日期：2010-02-01 00:00:00

abstract：:The genes involved in gluconate catabolism (gntP and gntK) in Corynebacterium glutamicum are scattered in the chromosome, and no regulatory genes are apparently associated with them, in contrast with the organization of the gnt operon in Escherichia coli and Bacillus subtilis. In C. glutamicum, gntP and gntK are essen...

journal_title：Journal of bacteriology

authors： Letek M,Valbuena N,Ramos A,Ordóñez E,Gil JA,Mateos LM

更新日期：2006-01-01 00:00:00

abstract：:Video techniques were used to record changes in motility of cells of Streptococcus sp. strain V4051 exposed to a variety of protein modification reagents. Starved cells were tethered to glass by a single flagellum, energized metabolically with glucose, or treated with valinomycin and energized artificially via shifts ...

journal_title：Journal of bacteriology

authors： Conley MP,Berg HC

更新日期：1984-06-01 00:00:00

abstract：:Overexpression of evgA, a response regulator of a two-component system, increased multidrug efflux in Escherichia coli. Since overexpression of the emrKY operon, which is controlled by evgAS, could account only for deoxycholate resistance, the evgAS locus apparently controls expression of at least one other multidrug ...

journal_title：Journal of bacteriology

authors： Nishino K,Yamaguchi A

更新日期：2001-02-01 00:00:00

abstract：:Envelope fractions isolated from Escherichia coli K-12 C600 and from colicin-resistant and colicin-tolerant (Tol II) mutants derived from this strain were separated on sucrose gradients into cell wall-enriched and cytoplasmic membrane-enriched fractions. These fractions were tested for their ability to neutralize coli...

journal_title：Journal of bacteriology

authors： Sabet SF,Schnaitman CA

更新日期：1971-10-01 00:00:00

abstract：:Pseudomonas sp. VLB120 uses styrene as a sole source of carbon and energy. The first step in this metabolic pathway is catalyzed by an oxygenase (StyA) and a NADH-flavin oxidoreductase (StyB). Both components have been isolated from wild-type Pseudomonas strain VLB120 as well as from recombinant Escherichia coli. StyA...

journal_title：Journal of bacteriology

authors： Otto K,Hofstetter K,Röthlisberger M,Witholt B,Schmid A

更新日期：2004-08-01 00:00:00

abstract：:The Staphylococcus aureus cidABC and lrgAB operons have been shown to regulate murein hydrolase activity and affect antibiotic tolerance. The cid operon enhances murein hydrolase activity and antibiotic sensitivity, whereas the lrg operon inhibits these processes. Based on these findings and the structural similaritie...

journal_title：Journal of bacteriology

authors： Yang SJ,Rice KC,Brown RJ,Patton TG,Liou LE,Park YH,Bayles KW

更新日期：2005-09-01 00:00:00

abstract：:Some bacteriophages target potentially pathogenic bacteria by exploiting surface-associated virulence factors as receptors. For example, phage have been identified that exhibit specificity for Vi capsule producing Salmonella enterica serovar Typhi. Here we have characterized the Vi-associated E1-typing bacteriophage u...

journal_title：Journal of bacteriology

authors： Pickard D,Thomson NR,Baker S,Wain J,Pardo M,Goulding D,Hamlin N,Choudhary J,Threfall J,Dougan G

更新日期：2008-04-01 00:00:00

abstract：:Uracil-DNA glycosylase activity was found in Streptococcus pneumoniae, and the enzyme was partially purified. An ung mutant lacking the activity was obtained by positive selection of cells transformed with a plasmid containing uracil in its DNA. The effects of the ung mutation on mutagenic processes in S. pneumoniae w...

journal_title：Journal of bacteriology

authors： Chen JD,Lacks SA

更新日期：1991-01-01 00:00:00

abstract：:In coupling the reducing power of illuminated chloroplasts to the nitrogenase from photosynthetically grown Rhodospirillum rubrum cells, one of the native ferredoxins. FdI, was found to be three times more effective than FdII. ...

journal_title：Journal of bacteriology

authors： Yoch DC,Arnon DI

更新日期：1975-02-01 00:00:00

abstract：:Transcription-coupled repair (TCR) is a cellular process by which some forms of DNA damage are repaired more rapidly from transcribed strands of active genes than from nontranscribed strands or the overall genome. In humans, the TCR coupling factor, CSB, plays a critical role in restoring transcription following both ...

journal_title：Journal of bacteriology

authors： Schalow BJ,Courcelle CT,Courcelle J

更新日期：2012-05-01 00:00:00

abstract：:Microorganisms that use sulfate as a terminal electron acceptor for anaerobic respiration play a central role in the global sulfur cycle. Here, we report the results of comparative sequence analysis of dissimilatory sulfite reductase (DSR) genes from closely and distantly related sulfate-reducing organisms to infer th...

journal_title：Journal of bacteriology

authors： Wagner M,Roger AJ,Flax JL,Brusseau GA,Stahl DA

更新日期：1998-06-01 00:00:00

abstract：:Changes in the cell wall of a species of Flavobacterium can be quantitated by measuring vancomycin adsorption. Treatment of the cells with ethylenediaminetetraacetic acid or tris(hydroxymethyl)aminomethane (or both) increased adsorption of the antibiotic. ...

journal_title：Journal of bacteriology

authors： Haslam DF,Best GK,Durham NN

更新日期：1970-08-01 00:00:00

abstract：:Falcone, Giuseppe (Rutgers, The State University, New Brunswick, N.J.), and Walter J. Nickerson. Reduction of selenite by intact yeast cells and cell-free preparations. J. Bacteriol. 85:754-762. 1963.-Nonproliferating cell suspensions of Candida albicans rapidly reduced selenite to red, metallic selenium in the absenc...

journal_title：Journal of bacteriology

authors： FALCONE G,NICKERSON WJ

更新日期：1963-04-01 00:00:00

abstract：:Protein 1, a major protein of the outer membrane of Escherichia coli, has been shown to be the pore allowing the passage of small hydrophilic solutes across the outer membrane. In E. coli K-12 protein 1 consists of two subspecies, 1a and 1b, whereas in E. coli B it consists of a single species which has an electrophor...

journal_title：Journal of bacteriology

authors： Pugsley AP,Schnaitman CA

更新日期：1978-03-01 00:00:00

abstract：:The ompR and envZ genes, which together constitute the ompB operon, are involved in osmoregulatory expression of the OmpF and OmpC proteins, major outer membrane proteins of Escherichia coli. The envZ11 mutation results in the OmpF- OmpC-constitutive phenotype. A mutant which suppressed defects caused by the envZ11 mu...

journal_title：Journal of bacteriology

authors： Matsuyama S,Mizuno T,Mizushima S

更新日期：1986-12-01 00:00:00

abstract：:The polycistronic mRNA encoding the nine genes of the unc operon of Escherichia coli was studied. We demonstrated the ribosome-binding capabilities of six of the nine unc genes, uncB, uncE, uncF, uncH, uncA, and uncD, by using the technique of primer extension inhibition or "toeprinting." No toeprint was detected for ...

journal_title：Journal of bacteriology

authors： Schaefer EM,Hartz D,Gold L,Simoni RD

更新日期：1989-07-01 00:00:00

abstract：:DnaA is an AAA+ ATPase and the conserved replication initiator in bacteria. Bacteria control the timing of replication initiation by regulating the activity of DnaA. DnaA binds to multiple sites in the origin of replication (oriC) and is required for recruitment of proteins needed to load the replicative helicase. Dna...

journal_title：Journal of bacteriology

authors： Bonilla CY,Grossman AD

更新日期：2012-09-01 00:00:00

abstract：:Flavobacterium johnsoniae cells glide rapidly over surfaces by an unknown mechanism. Transposon-induced sprA mutants formed nonspreading colonies on agar, and the cells examined in wet mounts were deficient in attachment to surfaces and were almost completely nonmotile. Exposure of intact cells to proteinase K cleaved...

journal_title：Journal of bacteriology

authors： Nelson SS,Glocka PP,Agarwal S,Grimm DP,McBride MJ

更新日期：2007-10-01 00:00:00

abstract：:A rough (R) Brucella abortus 45/20 mutant was more sensitive to the bactericidal activity of polymyxin B and lactoferricin B than was its smooth (S) counterpart but considerably more resistant than Salmonella montevideo. The outer membrane (OM) and isolated lipopolysaccharide (LPS) of S. montevideo showed a higher aff...

journal_title：Journal of bacteriology

authors： Freer E,Moreno E,Moriyón I,Pizarro-Cerdá J,Weintraub A,Gorvel JP

更新日期：1996-10-01 00:00:00

abstract：:Scanning electron microscopy has been employed to examine events in the release and development of mitospores of the aquatic fungus, Allomyces arbuscula. Among the salient features of spore release from the mitosporangium is the digestion of the inner matrix of the exit papillum. Hydrolysis appears to begin at the out...

journal_title：Journal of bacteriology

authors： Seale TW,Delehanty J,Runyan RB

更新日期：1974-12-01 00:00:00

abstract：:Streptococcus bovis possesses two sugar phosphate phosphatases (Pases). Pase I is a soluble enzyme that is inhibited by the membrane fractions from lactose-grown cells and is insensitive to activation by S46D HPr, an analog of HPr(ser-P) of the sugar phosphotransferase system. Pase II is a membrane-associated enzyme t...

journal_title：Journal of bacteriology

authors： Cook GM,Ye JJ,Russell JB,Saier MH Jr

更新日期：1995-12-01 00:00:00

abstract：:The mocC gene encoded by the octopine/mannityl opine-type Ti plasmid pTi15955 is related at the nucleotide sequence level to mas1' encoded by the T region of this plasmid. While Mas1 is required for the synthesis of mannopine (MOP) by crown gall tumor cells, MocC is essential for the utilization of MOP by Agrobacteriu...

journal_title：Journal of bacteriology

authors： Kim KS,Chilton WS,Farrand SK

更新日期：1996-06-01 00:00:00

abstract：:Normal animal sera inhibit at least one Clostridium histolyticum proteinase. An assay procedure based on immune hemolysis was developed for the estimation of this inhibition. This inhibitory activity occurs in various levels in the sera of different animal species. The highest titers have been obtained with rat sera. ...

journal_title：Journal of bacteriology

authors： Luzzati A,Goldlust MB,Levine L

更新日期：1968-12-01 00:00:00

abstract：:In the course of a study of genes located at min 44 of the Escherichia coli genome, we identified an open reading frame with the capacity to encode a 43-kDa polypeptide whose predicted amino acid sequence is strikingly similar to those of the well-known DD-carboxipeptidases penicillin-binding proteins PBP5 and PBP6. T...

journal_title：Journal of bacteriology

authors： Baquero MR,Bouzon M,Quintela JC,Ayala JA,Moreno F

更新日期：1996-12-01 00:00:00