Molecular characterization of the Salmonella enterica serovar Typhi Vi-typing bacteriophage E1.


:Some bacteriophages target potentially pathogenic bacteria by exploiting surface-associated virulence factors as receptors. For example, phage have been identified that exhibit specificity for Vi capsule producing Salmonella enterica serovar Typhi. Here we have characterized the Vi-associated E1-typing bacteriophage using a number of molecular approaches. The absolute requirement for Vi capsule expression for infectivity was demonstrated using different Vi-negative S. enterica derivatives. The phage particles were shown to have an icosahedral head and a long noncontractile tail structure. The genome is 45,362 bp in length with defined capsid and tail regions that exhibit significant homology to the S. enterica transducing phage ES18. Mass spectrometry was used to confirm the presence of a number of hypothetical proteins in the Vi phage E1 particle and demonstrate that a number of phage proteins are modified posttranslationally. The genome of the Vi phage E1 is significantly related to other bacteriophages belonging to the same serovar Typhi phage-typing set, and we demonstrate a role for phage DNA modification in determining host specificity.


J Bacteriol


Journal of bacteriology


Pickard D,Thomson NR,Baker S,Wain J,Pardo M,Goulding D,Hamlin N,Choudhary J,Threfall J,Dougan G




Has Abstract


2008-04-01 00:00:00














  • Mechanical properties of Bacillus subtilis cell walls: effects of removing residual culture medium.

    abstract::Experiments are described in which the tensile strength, the initial (Youngs') modulus, and other mechanical properties of the bacterial cell wall were obtained as functions of relative humidity (RH) in the range of 20 to 95%. These properties were deduced from tensile tests on bacterial thread, a fiber consisting of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Thwaites JJ,Surana UC

    更新日期:1991-01-01 00:00:00

  • Impaired expression of certain prereplicative bacteriophage T4 genes explains impaired T4 DNA synthesis in Escherichia coli rho (nusD) mutants.

    abstract::The Escherichia coli rho 026 mutation that alters the transcription termination protein Rho prevents growth of wild-type bacteriophage T4. Among the consequences of this mutation are delayed and reduced T4 DNA replication. We show that these defects can be explained by defective synthesis of certain T4 replication-rec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Stitt BL,Mosig G

    更新日期:1989-07-01 00:00:00

  • Succinic Semialdehyde Promotes Prosurvival Capability of Agrobacterium tumefaciens.

    abstract:UNLABELLED:Succinic semialdehyde (SSA), an important metabolite of γ-aminobutyric acid (GABA), is a ligand of the repressor AttJ regulating the expression of the attJ-attKLM gene cluster in the plant pathogen Agrobacterium tumefaciens. While the response of A. tumefaciens to GABA and the function of attKLM have been ex...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wang C,Tang D,Gao YG,Zhang LH

    更新日期:2016-01-11 00:00:00

  • Formation of the K30 (group I) capsule in Escherichia coli O9:K30 does not require attachment to lipopolysaccharide lipid A-core.

    abstract::Escherichia coli K antigens (capsular polysaccharides) are divided into two broad classes, designated groups I and II, on the basis of a number of chemical, physical, and genetic criteria. Group I K antigens can be further subdivided on the basis of the absence (group IA) or presence (group IB) of amino sugars in the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: MacLachlan PR,Keenleyside WJ,Dodgson C,Whitfield C

    更新日期:1993-12-01 00:00:00

  • Overlapping mRNA transcripts of photosynthesis gene operons in Rhodobacter capsulatus.

    abstract::The crtEF, bchCA, and puf operons of the facultative phototrophic bacterium Rhodobacter capsulatus encode gene products that are necessary for the formation of various components of the photosynthetic apparatus. The crtEF operon encodes two enzymes involved in the biosynthesis of carotenoids, the bchCA operon codes fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wellington CL,Beatty JT

    更新日期:1991-02-01 00:00:00

  • Ultrastructure of Treponema microdentium and Borrelia vincentii.

    abstract::Bladen, Howard A. (National Institute of Dental Health, Bethesda, Md.), and Edward G. Hampp. Ultrastructure of Treponema microdentium and Borrelia vincentii. J. Bacteriol. 87:1180-1191.-A small oral Treponema (FM) and Borrelia vincentii (N9) were harvested after 3 to 7 days of incubation and either embedded in Vestopa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bladen HA,Hampp EG

    更新日期:1964-05-01 00:00:00

  • The primosomal protein DnaD inhibits cooperative DNA binding by the replication initiator DnaA in Bacillus subtilis.

    abstract::DnaA is an AAA+ ATPase and the conserved replication initiator in bacteria. Bacteria control the timing of replication initiation by regulating the activity of DnaA. DnaA binds to multiple sites in the origin of replication (oriC) and is required for recruitment of proteins needed to load the replicative helicase. Dna...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bonilla CY,Grossman AD

    更新日期:2012-09-01 00:00:00

  • Expression of cspH, encoding the cold shock protein in Salmonella enterica serovar Typhimurium UK-1.

    abstract::Both Salmonella enterica serovar Typhimurium and Escherichia coli contain the cspH gene encoding CspH, one of the cold shock proteins (CSPs). In this study, we investigated the expression of cspH in S. enterica serovar Typhimurium and found that it was induced in response to a temperature downshift during exponential ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kim BH,Bang IS,Lee SY,Hong SK,Bang SH,Lee IS,Park YK

    更新日期:2001-10-01 00:00:00

  • Escherichia coli mutants thermosensitive for deoxyribonucleic acid gyrase subunit A: effects on deoxyribonucleic acid replication, transcription, and bacteriophage growth.

    abstract::Temperature-sensitive nalA mutants of Escherichia coli have been used to investigate the structure and functions of deoxyribonucleic acid (DNA) gyrase. Extracts of one such mutant (nalA43) had thermosensitive DNA gyrase subunit A activity but normal gyrase subunit B activity, proving definitively that nalA is the stru...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kreuzer KN,Cozzarelli NR

    更新日期:1979-11-01 00:00:00

  • Coupling of asymmetric division to polar placement of replication origin regions in Bacillus subtilis.

    abstract::Entry into sporulation in Bacillus subtilis is characterized by the formation of a polar septum, which asymmetrically divides the developing cell into forespore (the smaller cell) and mother cell compartments, and by migration of replication origin regions to extreme opposite poles of the cell. Here we show that polar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Graumann PL,Losick R

    更新日期:2001-07-01 00:00:00


    abstract::Pizer, Lewis I. (University of Pennsylvania, Philadelphia). Glycine synthesis and metabolism in Escherichia coli. J. Bacteriol. 89:1145-1150. 1965.-A correlation was demonstrated between a nutritional requirement that can only be satisfied by glycine and the absence of the enzymatic capacity to interconvert l-serine a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: PIZER LI

    更新日期:1965-04-01 00:00:00

  • Genetic mapping of the chromosomal replication origin of Salmonella typhimurium.

    abstract::Two hundred strains of Escherichia coli harboring Filv+ plasmids which carry a segment of the Salmonella typhimurium chromosome were isolated independently. Among them, two strains were found to harbor F' plasmids that are able to replicate in Hfr cells of E. coli; i.e., they carry a site designated poh (permissive on...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Joh K,Hiraga S

    更新日期:1979-05-01 00:00:00

  • Mutants of yeast sensitive to ultraviolet light.

    abstract::Six uvr mutants of Saccharomyces cerevisiae with hypersensitivity to ultraviolet (UV) light were isolated after mutagen treatment with ethylmethanesulfonate. UV sensitivity ranges from moderate to extreme, and four of the mutants are also sensitive to nitrous acid. Ranking in terms of UV sensitivity does not parallel ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Snow R

    更新日期:1967-09-01 00:00:00

  • Nucleotide sequence and expression of cheF, an essential gene for chemotaxis in Bacillus subtilis.

    abstract::The cheF gene, which is involved in chemotaxis in Bacillus subtilis, has been cloned, expressed, and sequenced. This gene is contained in a 0.7-kilobase PstI DNA fragment that was isolated from a lambda Charon 4A B. subtilis chromosomal DNA library. This fragment was subcloned into the expression vector pSI-1 and show...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ying CW,Ordal GW

    更新日期:1989-03-01 00:00:00

  • Chlamydial hemagglutinin identified as lipopolysaccharide.

    abstract::Chlamydial lipopolysaccharide (LPS) agglutinated mouse and rabbit erythrocytes but not human, guinea pig, or pronghorn antelope erythrocytes. Hemagglutination was not specific for Chlamydia spp., as rough LPSs from Coxiella burnetii and Escherichia coli also agglutinated erythrocytes from the same animal species. Nona...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Watkins NG,Caldwell HD,Hackstadt T

    更新日期:1987-08-01 00:00:00

  • Hypersensitivity to Hg2+ and hyperbinding activity associated with cloned fragments of the mercurial resistance operon of plasmid NR1.

    abstract::The region of plasmid NR1 concerned with resistance to Hg2+ and organomercurials consists of sequences found on restriction endonuclease fragments EcoRI-H and EcoRI-I. When both fragments were cloned together into a derivative of plasmid ColE1, the hybrid plasmid conferred properties indistinguishable from those of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Nakahara H,Silver S,Miki T,Rownd RH

    更新日期:1979-10-01 00:00:00

  • Streptococcus faecium ATCC 9790 penicillin-binding proteins and penicillin sensitivity are heavily influenced by growth conditions: proposal for an indirect mechanism of growth inhibition by beta-lactams.

    abstract::The effects of variations in growth conditions on the penicillin response of Streptococcus faecium ATCC 9790 were studied. Changes in the growth temperature and medium composition were found to cause striking changes in the bacterial generation time, cellular penicillin sensitivity (minimum inhibitory concentration), ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Fontana R,Canepari P,Satta G,Coyette J

    更新日期:1983-05-01 00:00:00

  • YycH and YycI interact to regulate the essential YycFG two-component system in Bacillus subtilis.

    abstract::The YycFG two-component system is the only signal transduction system in Bacillus subtilis known to be essential for cell viability. This system is highly conserved in low-G+C gram-positive bacteria, regulating important processes such as cell wall homeostasis, cell membrane integrity, and cell division. Four other ge...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Szurmant H,Mohan MA,Imus PM,Hoch JA

    更新日期:2007-04-01 00:00:00

  • Characteristics of some multiply recombination-deficient strains of Escherichia coli.

    abstract::Strains of Escherichia coli have been made carrying lesions in more than one gene determining recombination. The following genotypes were constructed and verified: recC22 recB21 recA(+), recC22 recB21 recA13, recC22 recB(+)recA13, and recC(+)recB21 recA13. All multiple rec(-) strains carrying recA13 were similar to AB...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Willetts NS,Clark AJ

    更新日期:1969-10-01 00:00:00

  • Identification and characterization of CAMP cohemolysin as a potential virulence factor of Riemerella anatipestifer.

    abstract::Riemerella anatipestifer is responsible for exudative septicemia in ducks. The genetic determinant of the CAMP cohemolysin, cam, from a strain of R. anatipestifer was cloned and expressed in Escherichia coli. Chromosomal DNA from serotype 19 strain 30/90 was used to construct a gene library in pBluescript II SK(-) vec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Crasta KC,Chua KL,Subramaniam S,Frey J,Loh H,Tan HM

    更新日期:2002-04-01 00:00:00

  • RNA-Mediated cis Regulation in Acinetobacter baumannii Modulates Stress-Induced Phenotypic Variation.

    abstract::In the nosocomial opportunistic pathogen Acinetobacter baumannii, RecA-dependent mutagenesis, which causes antibiotic resistance acquisition, is linked to the DNA damage response (DDR). Notably, unlike the Escherichia coli paradigm, recA and DDR gene expression in A. baumannii is bimodal. Namely, there is phenotypic v...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ching C,Gozzi K,Heinemann B,Chai Y,Godoy VG

    更新日期:2017-05-09 00:00:00

  • Regulation of Staphylococcus xylosus xylose utilization genes at the molecular level.

    abstract::We have investigated the regulation of the operon encoding xylose utilization in Staphylococcus xylosus C2a and Staphylococcus carnosus TM300. For in vivo studies, transcriptional fusions of the xylAB regulatory region to the lipase gene from Staphylococcus hyicus were constructed. Repression of lipase activity depend...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sizemore C,Wieland B,Götz F,Hillen W

    更新日期:1992-05-01 00:00:00

  • In vivo rearrangement of foreign DNA by Fusarium oxysporum produces linear self-replicating plasmids.

    abstract::Particular combinations of fungal strains and transformation vectors allow for fungal rearrangement of normally integrative plasmids, resulting in the creation of linear self-replicating plasmids in Fusarium oxysporum. The rearrangement results in the addition of fungal DNA, including telomere consensus sequences, to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Powell WA,Kistler HC

    更新日期:1990-06-01 00:00:00

  • Molecular analysis and regulation of the glnA gene of the gram-positive anaerobe Clostridium acetobutylicum.

    abstract::The nucleotide sequence of a 2.0-kilobase DNA segment containing the Clostridium acetobutylicum glnA gene was determined. The upstream region of the glnA gene contained two putative extended promoter consensus sequences (p1 and p2), characteristic of gram-positive bacteria. A third putative extended gram-positive prom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Janssen PJ,Jones WA,Jones DT,Woods DR

    更新日期:1988-01-01 00:00:00

  • Protein and fatty acid composition of mesosomal vesicles and plasma membranes of Staphylococcus aureus.

    abstract::Qualitatively, the protein and fatty acid composition of purified mesosomal vesicles and the plasma membrane isolated from Staphylococcus aureus ATCC 6538P are identical, the major difference between these two cellular components being only quantitative in nature. Mesosomal vesicles and plasma membranes, when subjecte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Theodore TS,Panos C

    更新日期:1973-11-01 00:00:00

  • Messenger ribonucleic acid of dormant spores of Bacillus subtilis.

    abstract::Evidence of the presence of messenger ribonucleic acid (mRNA) in dormant spores of Bacillus subtilis has been obtained. The bulk RNA from spores was isolated and labeled in vitro with tritiated dimethyl sulfate. The spore RNA hybridized to 2.4 to 3.2% of the B. subtilis genome. The RNA hybridized to both the complemen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jeng YH,Doi RH

    更新日期:1974-08-01 00:00:00

  • Bioconversion of m-hydroxybenzoate to 2,3-dihydroxybenzoate by mutants of Pseudomonas testosteroni.

    abstract::Mutans of Pseudomonas testosteroni were isolated for their inability to grow on m-hydroxybenzoate as sole carbon source. These mutants hydroxylated m-hydroxybenzoate for form 2,3-dihydroxybenzoate in high yeilds. The bioconversion described in this report represents the first reported example of 3-hydroxybenzoate 2-hy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Daumy GO,McColl AS,Andrews GC

    更新日期:1980-01-01 00:00:00

  • Overexpression of a cloned IMP dehydrogenase gene of Candida albicans confers resistance to the specific inhibitor mycophenolic acid.

    abstract::An IMP dehydrogenase gene was isolated from Candida albicans on a approximately 2.9-kb XbaI genomic DNA fragment. The putative Candida IMP dehydrogenase gene (IMH3) encodes a protein of 521 amino acids with extensive sequence similarity to the IMP dehydrogenases of Saccharomyces cerevisiae and various other organisms....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Köhler GA,White TC,Agabian N

    更新日期:1997-04-01 00:00:00

  • Identification, cloning, and initial characterization of rot, a locus encoding a regulator of virulence factor expression in Staphylococcus aureus.

    abstract::A chromosomal insertion of transposon Tn917 partially restores the expression of protease and alpha-toxin activities to PM466, a genetically defined agr-null derivative of the wild-type Staphylococcus aureus strain RN6390. In co-transduction experiments, transposon-encoded erythromycin resistance and a protease- and a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McNamara PJ,Milligan-Monroe KC,Khalili S,Proctor RA

    更新日期:2000-06-01 00:00:00

  • Genome sequence of a dominant, multidrug-resistant Acinetobacter baumannii strain, TCDC-AB0715.

    abstract::Acinetobacter baumannii has emerged as a significant nosocomial pathogen worldwide. The increasing trend of carbapenem and fluoroquinolone resistance in A. baumannii severely limits the usage of therapeutic antimicrobial agents. Here we report the genome sequence of a multidrug-resistant A. baumannii strain, TCDC-AB07...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chen CC,Lin YC,Sheng WH,Chen YC,Chang SC,Hsia KC,Liao MH,Li SY

    更新日期:2011-05-01 00:00:00