Genetic, molecular, and functional analysis of Streptococcus faecalis R plasmid pJH1.

Abstract:

:Streptococcus faecalis JH1 contains two conjugative plasmids, pJH1, an R plasmid that codes for resistance to kanamycin, streptomycin, erythromycin, and tetracycline, and pJH2, a hemolysin-bacteriocin plasmid. Strain JH1 was used as an antibiotic resistance donor in conjugation experiments with two plasmid-free S. faecalis recipient strains, JH2-2 and OG1-RF1. Plasmid pJH1 was purified from one transconjugant, DL77, and subjected to restriction endonuclease analyses. Five restriction enzymes, EcoRI, XbaI, BamHI, SalI, and XhoI, yielding 10, 9, 3, 2, and 2 fragments, respectively, were used to determine the size (80.7 kilobases) of pJH1 and to construct a restriction endonuclease map of the plasmid. Twenty-eight percent of the antibiotic-resistant transconjugants examined expressed only part of the resistance pattern (Kmr Smr Emr Tcr) associated with pJH1, that is, they were resistant to kanamycin, streptomycin, and erythromycin; to erythromycin and tetracycline; or to erythromycin or to tetracycline only. Most of these strains also produced hemolysin and bacteriocin, and several contained a hybrid plasmid consisting of pJH2 and specific segments of pJH1 DNA. Several of these hybrid plasmids, as well as a deletion derivative of pJH1 that coded for resistance to tetracycline but not to kanamycin, streptomycin, or erythromycin, were purified and used to confirm the arrangement of restriction endonuclease fragments on the pJH1 map and to locate the resistance determinants on this map.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Banai M,LeBlanc DJ

doi

10.1128/JB.155.3.1094-1104.1983

subject

Has Abstract

pub_date

1983-09-01 00:00:00

pages

1094-104

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

155

pub_type

杂志文章
  • Cloning and promoter identification of the iron-regulated cir gene of Escherichia coli.

    abstract::The cir gene, which encodes the colicin I receptor protein and is regulated by both cellular iron content and growth temperature, was cloned into a multicopy-number plasmid. Physical mapping and complementation analysis established the position of cir between mgl and nfo on the Escherichia coli chromosome. A gene enco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.12.5343-5352.1987

    authors: Griggs DW,Tharp BB,Konisky J

    更新日期:1987-12-01 00:00:00

  • Functionally essential interaction between Yersinia YscO and the T3S4 domain of YscP.

    abstract::The type III secretion (T3S) system is essential to the virulence of a large number of Gram-negative bacterial pathogens, including Yersinia. YscO is required for T3S in Yersinia and is known to interact with several other T3S proteins, including the chaperone SycD and the needle length regulator YscP. To define which...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00876-13

    authors: Mukerjea R,Ghosh P

    更新日期:2013-10-01 00:00:00

  • High-molecular-weight complexes of RsbR and paralogues in the environmental signaling pathway of Bacillus subtilis.

    abstract::Bacillus subtilis has developed an intricate signal transduction cascade to respond to the imposition of a variety of stresses on the cell. Reversible protein phosphorylation and the formation of alternative protein-protein complexes modulate the activity of sigma(B), the RNA polymerase sigma factor subunit responsibl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00892-06

    authors: Delumeau O,Chen CC,Murray JW,Yudkin MD,Lewis RJ

    更新日期:2006-11-01 00:00:00

  • Identification of a linked set of genes in Serpulina hyodysenteriae (B204) predicted to encode closely related 39-kilodalton extracytoplasmic proteins.

    abstract::A tandem pair of nearly identical genes from Serpulina hyodysenteriae (B204) were cloned and sequenced. The full open reading frame of one gene and the partial open reading frame of the neighboring gene appear to encode secreted proteins which are homologous to, yet distinct from, the 39-kDa extracytoplasmic protein p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.2.444-448.1998

    authors: Gabe JD,Dragon E,Chang RJ,McCaman MT

    更新日期:1998-01-01 00:00:00

  • Identification of phosphate starvation-inducible genes in Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu d1) transcriptional fusions.

    abstract::Twenty-four independent phosphate starvation-inducible (psi) transcriptional fusions made with Mu d1(lacZbla) were analyzed by sequencing the psi::lacZ(Mu d1) chromosomal junctions by using DNAs amplified with the polymerase chain reaction or mini-Mu cloning. Our DNA sequence analysis showed that the MuR DNA in Mu d1 ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.6.3191-3200.1990

    authors: Metcalf WW,Steed PM,Wanner BL

    更新日期:1990-06-01 00:00:00

  • Phospholipid biosynthesis in some anaerobic bacteria.

    abstract::We have identified and characterized enzymes of phospholipid synthesis in two plasmalogen-rich anaerobes. Megasphaera elsdenii and Veillonella parvula, and one anaerobe lacking plasmalogens. Desulfovibrio vulgaris. All three species contained phosphatidate cytidylyltransferase and phosphatidylserine synthase. Phosphat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.147.1.57-61.1981

    authors: Silber P,Borie RP,Mikowski EJ,Goldfine H

    更新日期:1981-07-01 00:00:00

  • Effect of 1,10-phenanthroline on bacterial conjugation in Escherichia coli K-12: inhibition of maturation from preliminary mates into effective mates.

    abstract::The addition of 1 mM orthophenanthroline (OP) into a mating mixture drastically reduced the production of recombinants. Examination of the effect of OP on each step of conjugation showed that the effect on the following steps could not account for the up to 500-fold reduction of recombinant formation: (i) preliminary ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.128.1.363-371.1976

    authors: Ou JT,Reim R

    更新日期:1976-10-01 00:00:00

  • The central domain of Rhizobium leguminosarum DctD functions independently to activate transcription.

    abstract::Sigma 54-dependent transcriptional activators such as Escherichia coli NtrC, Rhizobium meliloti NifA, and Rhizobium leguminosarum DctD share similar central and carboxy-terminal domains but differ in the structure and function of their amino-terminal domains. We have deleted the amino-terminal and carboxy-terminal dom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.4.1428-1431.1992

    authors: Huala E,Stigter J,Ausubel FM

    更新日期:1992-02-01 00:00:00

  • Role of acid pH and deficient efflux of pyrazinoic acid in unique susceptibility of Mycobacterium tuberculosis to pyrazinamide.

    abstract::Pyrazinamide (PZA) is an important antituberculosis drug. Unlike most antibacterial agents, PZA, despite its remarkable in vivo activity, has no activity against Mycobacterium tuberculosis in vitro except at an acidic pH. M. tuberculosis is uniquely susceptible to PZA, but other mycobacteria as well as nonmycobacteria...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.7.2044-2049.1999

    authors: Zhang Y,Scorpio A,Nikaido H,Sun Z

    更新日期:1999-04-01 00:00:00

  • Structure-based functional analysis of the replication protein of plasmid R6K: key amino acids at the pi/DNA interface.

    abstract::In previous work, we characterized the bases in an iteron of plasmid R6K that are important for the binding of pi protein monomers and dimers. Here we investigate the following six amino acids of pi, encoded by pir, hypothesized to be important for DNA contact: Ser71, Try74, Gly131, Gly211, Arg225, and Arg254. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00109-07

    authors: Kunnimalaiyaan S,Rakowski SA,Filutowicz M

    更新日期:2007-07-01 00:00:00

  • The "green" form I ribulose 1,5-bisphosphate carboxylase/oxygenase from the nonsulfur purple bacterium Rhodobacter capsulatus.

    abstract::Form I ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) of the Calvin-Benson-Bassham cycle may be divided into two broad phylogenetic groups, referred to as red-like and green-like, based on deduced large subunit amino acid sequences. Unlike the form I enzyme from the closely related organism Rhodobacter spha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.13.3935-3941.1999

    authors: Horken KM,Tabita FR

    更新日期:1999-07-01 00:00:00

  • Induction and control of the autolytic system of Escherichia coli.

    abstract::Various methods of inducing autolysis of Escherichia coli cells were investigated, some being described here for the first time. For the autolysis of growing cells only induction methods interfering with the biosynthesis of peptidoglycan were taken into consideration, whereas with harvested cells autolysis was induced...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Leduc M,Kasra R,van Heijenoort J

    更新日期:1982-10-01 00:00:00

  • Membrane chaperone SecDF plays a role in the secretion of Listeria monocytogenes major virulence factors.

    abstract::Listeria monocytogenes is a Gram-positive human intracellular pathogen that infects diverse mammalian cells. Upon invasion, L. monocytogenes secretes multiple virulence factors that target host cellular processes and promote infection. It has been presumed, but was not empirically established, that the Sec translocati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00697-13

    authors: Burg-Golani T,Pozniak Y,Rabinovich L,Sigal N,Nir Paz R,Herskovits AA

    更新日期:2013-12-01 00:00:00

  • TOL plasmid in Pseudomonas aeruginosa PAO: thermosensitivity of self-maintenance and inhibition of host cell growth.

    abstract::The TOL plasmid originally isolated in Pseudomonas putida (arvilla) mt-2 was transmissible to strains of the fluorescens group of Pseudomonas, i.e., P. putida, P. fluorescens, and P. aeruginosa, except for a strain of P. aeruginosa, strain PAO. The same strain, however, could accept the plasmid when its restriction an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.2.527-535.1978

    authors: Nakazawa T

    更新日期:1978-02-01 00:00:00

  • Transcriptional phase variation of a type III restriction-modification system in Helicobacter pylori.

    abstract::Phase variation is important in bacterial pathogenesis, since it generates antigenic variation for the evasion of immune responses and provides a strategy for quick adaptation to environmental changes. In this study, a Helicobacter pylori clone, designated MOD525, was identified that displayed phase-variable lacZ expr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.23.6615-6624.2002

    authors: de Vries N,Duinsbergen D,Kuipers EJ,Pot RG,Wiesenekker P,Penn CW,van Vliet AH,Vandenbroucke-Grauls CM,Kusters JG

    更新日期:2002-12-01 00:00:00

  • Evolutionary and diagnostic implications of intragenomic heterogeneity in the 16S rRNA gene in Aeromonas strains.

    abstract::Sequencing 16S rRNA genes (SSU) cloned from Aeromonas strains revealed that strains contained up to six copies differing by < or = 1.5%. The SSU copies from Aeromonas veronii LMG13695 clustered with sequences from four Aeromonas species. These results demonstrate intragenomic heterogeneity of SSU and suggest caution w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.18.6561-6564.2005

    authors: Morandi A,Zhaxybayeva O,Gogarten JP,Graf J

    更新日期:2005-09-01 00:00:00

  • Genetics of methyl-accepting chemotaxis proteins in Escherichia coli: cheD mutations affect the structure and function of the Tsr transducer.

    abstract::The tsr gene specifies a methyl-accepting membrane protein involved in chemotaxis to serine and several repellent compounds. We have characterized a special class of tsr mutations designated cheD which alter the signaling properties of the Tsr transducer. Unlike tsr null mutants, cheD strains are generally nonchemotac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.96-104.1985

    authors: Callahan AM,Parkinson JS

    更新日期:1985-01-01 00:00:00

  • Repression of nitrate reductase in Neurospora studied by using L-methionine-DL-sulfoximine and glutamine auxotroph gln-1b.

    abstract::The effect of L-methionine-DL-sulfoximine, an inhibitor of glutamine synthetase, on the formation of nitrate reductase in the wild-type strain of Neurospora in the presence of ammonium ions and of glutamine was studied. Under conditions in which glutamine synthetase was inactivated, it was found that only glutamine co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.1.411-415.1980

    authors: Premakumar R,Sorger GJ,Gooden D

    更新日期:1980-07-01 00:00:00

  • Bacillus subtilis ccpA gene mutants specifically defective in activation of acetoin biosynthesis.

    abstract::A large number of carbon source utilization pathways are repressed in Bacillus subtilis by the global regulator CcpA, which also acts as an activator of carbon excretion pathways during growth in media containing glucose. In this study, CcpA mutants defective in transcriptional activation of the alsSD operon, which is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.19.5611-5614.2000

    authors: Turinsky AJ,Moir-Blais TR,Grundy FJ,Henkin TM

    更新日期:2000-10-01 00:00:00

  • Utilization of octopine and nopaline by Agrobacterium.

    abstract::Tests for utilization of d-octopine and nopaline in defined media containing a carbon and nitrogen source were made on 60 strains of Agrobacterium representing four species and on a representative of each of five species of Rhizobium. Among 46 virulent strains of Agrobacterium, only two strains were found which utiliz...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.1.378-383.1973

    authors: Lippincott JA,Beiderbeck R,Lippincott BB

    更新日期:1973-10-01 00:00:00

  • The cyclic nucleotide monophosphate domain of Xanthomonas campestris global regulator Clp defines a new class of cyclic di-GMP effectors.

    abstract::The widely conserved second messenger cyclic diguanosine monophosphate (c-di-GMP) plays a key role in quorum-sensing (QS)-dependent production of virulence factors in Xanthomonas campestris pv. campestris. The detection of QS diffusible signal factor (DSF) by the sensor RpfC leads to the activation of response regulat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01253-09

    authors: Tao F,He YW,Wu DH,Swarup S,Zhang LH

    更新日期:2010-02-01 00:00:00

  • Host-induced modification-restriction and the utilization of 5-bromouracil by thymineless mutants of Escherichia coli.

    abstract::An m(k) (-)r(k) (-) mutation exerted no measurable effect on 5-bromouracil incorporation by a thymineless derivative of Escherichia coli K. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.606-607.1970

    authors: Matney TS,MacDougall NL,Butler MA,Suit JC

    更新日期:1970-10-01 00:00:00

  • Flexible Hinges in Bacterial Chemoreceptors.

    abstract::Transmembrane bacterial chemoreceptors are extended, rod-shaped homodimers with ligand-binding sites at one end and interaction sites for signaling complex formation and histidine kinase control at the other. There are atomic-resolution structures of chemoreceptor fragments but not of intact, membrane-inserted recepto...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00593-17

    authors: Akkaladevi N,Bunyak F,Stalla D,White TA,Hazelbauer GL

    更新日期:2018-02-07 00:00:00

  • Role and regulation of Bacillus subtilis glutamate dehydrogenase genes.

    abstract::The complete Bacillus subtilis genome contains two genes with the potential to encode glutamate dehydrogenase (GlutDH) enzymes. Mutations in these genes were constructed and characterized. The rocG gene proved to encode a major GlutDH whose synthesis was induced in media containing arginine or ornithine or, to a lesse...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Belitsky BR,Sonenshein AL

    更新日期:1998-12-01 00:00:00

  • Deletion of the spf (spot 42 RNA) gene of Escherichia coli.

    abstract::To investigate the function of spot 42 RNA, a small RNA of Escherichia coli, we constructed a strain in which spf, the structural gene for this RNA, is deleted. We achieved this by using a delta att phage lambda carrying a DNA fragment spanning the spf region but with a precise deletion of spf. By integration of this ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.3.746-750.1986

    authors: Hatfull GF,Joyce CM

    更新日期:1986-06-01 00:00:00

  • Malate utilization by a group D Streptococcus: physiological properties and purification of an inducible malic enzyme.

    abstract::Growth of Streptococcus faecalis in the presence of l-malate resulted in the induction of a "malic enzyme" [l-malate:nicotinamide adenine dinucleotide (NAD) oxidoreductase (decarboxylating), E.C. 1.1.1.39]. Synthesis of the malic enzyme did not appear to be subject to catabolite repression by intermediate products of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.2.705-711.1969

    authors: London J,Meyer EY

    更新日期:1969-05-01 00:00:00

  • nodO, a new nod gene of the Rhizobium leguminosarum biovar viciae sym plasmid pRL1JI, encodes a secreted protein.

    abstract::The region of the Rhizobium leguminosarum biovar viciae Sym plasmid pRL1JI, responsible for the production and secretion of a previously described 50-kilodalton protein (R. A. de Maagd, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg, J. Bacteriol. 170:4424-4427, 1988), was cloned and its nucleotide sequence was de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.12.6764-6770.1989

    authors: de Maagd RA,Wijfjes AH,Spaink HP,Ruiz-Sainz JE,Wijffelman CA,Okker RJ,Lugtenberg BJ

    更新日期:1989-12-01 00:00:00

  • Site-specific integration and expression of a developmental promoter in Myxococcus xanthus.

    abstract::A series of intercellular signals are involved in the regulation of gene expression during fruiting body formation of Myxococcus xanthus. Mutations which block cell interactions, such as csgA (formerly known as spoC), also prevent expression of certain developmentally regulated promoters. csgA+ cells containing Tn5 la...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.12.5552-5556.1988

    authors: Li SF,Shimkets LJ

    更新日期:1988-12-01 00:00:00

  • Gliding motility and Por secretion system genes are widespread among members of the phylum bacteroidetes.

    abstract::The phylum Bacteroidetes is large and diverse, with rapid gliding motility and the ability to digest macromolecules associated with many genera and species. Recently, a novel protein secretion system, the Por secretion system (PorSS), was identified in two members of the phylum, the gliding bacterium Flavobacterium jo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01962-12

    authors: McBride MJ,Zhu Y

    更新日期:2013-01-01 00:00:00

  • Composition of cardiolipin molecular species in Escherichia coli.

    abstract::The composition of the molecular species of acidic phospholipids in Escherichia coli B during the late exponential growth phase at 37 degrees C was determined. Two phosphatidyl groups of cardiolipin, the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of cardiolipin, were isolated by limited hydrolysis with p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.3.1047-1051.1980

    authors: Yokota K,Kanamoto R,Kito M

    更新日期:1980-03-01 00:00:00