Overexpression of vsr in Escherichia coli is mutagenic.

abstract：:We have isolated and determined the nucleotide sequence of a 1,626-base-pair fragment from R-plasmid R483 which encodes a trimethoprim-resistant dihydrofolate reductase. Analysis of the nucleotide sequence of this fragment revealed the presence of two open reading frames, each sufficient to encode polypeptides of appr...

journal_title：Journal of bacteriology

authors： Simonsen CC,Chen EY,Levinson AD

更新日期：1983-09-01 00:00:00

abstract：:Genes involved in the biosynthesis of leucine have been mapped in Bacillus megaterium QM B1551, using transducing phage MP13. Mutations were designated leuA, leuB, or leuC on the basis of enzyme assays. Two mutant strains were deficient in the enzyme activities of leuA (alpha-isopropylmalate synthase) and leuC (beta-i...

journal_title：Journal of bacteriology

authors： Garbe JC,Hess GF,Franzen MA,Vary PS

更新日期：1984-02-01 00:00:00

abstract：:The isocitrate lyase from a thermophilic Bacillus is activated about threefold by a variety of salts. Such strong stimulation of activity is not seen with isocitrate lyase from the mesophiles, Bacillus licheniformis, Bacillus megaterium, Escherichia coli, and Aspergillus nidulans. The salt activation is markedly pH-de...

journal_title：Journal of bacteriology

authors： Griffiths MW,Sundaram TK

更新日期：1973-12-01 00:00:00

abstract：:Streptococcus agalactiae (group B streptococcus [GBS]) is a major cause of infections in newborns, pregnant women, and immunocompromised patients. GBS strain CNCTC10/84 is a clinical isolate that has high virulence in animal models of infection and has been used extensively to study GBS pathogenesis. Two unusual featu...

journal_title：Journal of bacteriology

authors： Zhu L,Beres SB,Yerramilli P,Pruitt L,Cantu CC,Olsen RJ,Musser JM

更新日期：2020-11-04 00:00:00

abstract：:Marchette, Nyven J. (University of Utah, Salt Lake City), and Paul S. Nicholes. Virulence and citrulline ureidase activity of Pasteurella tularensis. J. Bacteriol. 82:26-32. 1961.-The presence of a citrulline ureidase system in Pasteurella tularensis strains of high virulence, and its absence in avirulent strains and ...

journal_title：Journal of bacteriology

authors： MARCHETTE NJ,NICHOLES PS

更新日期：1961-07-01 00:00:00

abstract：:Citrate metabolism in Leuconostoc mesenteroides subspecies mesenteroides is associated with the generation of a proton motive force by a secondary mechanism (C. Marty-Teysset, C. Posthuma, J. S. Lolkema, P. Schmitt, C. Divies, and W. N. Konings, J. Bacteriol. 178:2178-2185, 1996). The pathway consists of four steps: (...

journal_title：Journal of bacteriology

authors： Marty-Teysset C,Lolkema JS,Schmitt P,Diviès C,Konings WN

更新日期：1996-11-01 00:00:00

abstract：:Livers of uninfected guinea pigs and of guinea pigs infected with Coxiella burneti were fractionated into smooth endoplasmic reticulum, rough endoplasmic reticulum (RER), pellet, and cell sap fractions. The ribonucleic acid (RNA) and protein of each fraction were determined, and the phosphorylase, glucose-6-phosphatas...

journal_title：Journal of bacteriology

authors： Paretsky D,Stueckemann J

更新日期：1970-05-01 00:00:00

abstract：:Bacillus subtilis, which grows under aerobic conditions, employs fatty acid desaturase (Des) to fluidize its membrane when subjected to temperature downshift. Des requires molecular oxygen for its activity, and its expression is regulated by DesK-DesR, a two-component system. Transcription of des is induced by the tem...

journal_title：Journal of bacteriology

authors： Beranová J,Mansilla MC,de Mendoza D,Elhottová D,Konopásek I

更新日期：2010-08-01 00:00:00

abstract：:Harada, Kenji (Gunma University, Maebashi, Japan), Mitsuo Kameda, Mitsue Suzuki, and Susumu Mitsuhashi. Drug resistance of enteric bacteria. II. Transduction of transmissible drug-resistance (R) factors with phage epsilon. J. Bacteriol. 86:1332-1338. 1963.-Transmissible drug-resistance (R) factors, which transfer resi...

journal_title：Journal of bacteriology

authors： HARADA K,KAMEDA M,SUZUKI M,MITSUHASHI S

更新日期：1963-12-01 00:00:00

abstract：:The CpxA-CpxR two-component signal transduction pathway of Escherichia coli was studied in a mutant (pss-93) lacking phosphatidylethanolamine (PE). Several properties of this mutant are comparable to phenotypes of cpxA point mutants, indicating that this two-component pathway is activated in PE-deficient cells. In con...

journal_title：Journal of bacteriology

authors： Mileykovskaya E,Dowhan W

更新日期：1997-02-01 00:00:00

abstract：:Rosenkranz, Herbert S. (Columbia University, New York, N.Y.), Howard S. Carr, and Harry M. Rose. Phenethyl alcohol. II. Effect on thymine-requiring Escherichia coli. J. Bacteriol. 89:1370-1373. 1965.-Phenethyl alcohol (PEA) at levels (0.05%) presumed to interfere with messenger ribonucleic acid synthesis also inhibits...

journal_title：Journal of bacteriology

authors： ROSENKRANZ HS,CARR HS,ROSE HM

更新日期：1965-05-01 00:00:00

abstract：:Legionella pneumophila is the causative agent of the severe and potentially fatal pneumonia Legionnaires' disease. L. pneumophila is able to replicate within macrophages and protozoa by establishing a replicative compartment in a process that requires the Icm/Dot type IVB secretion system. The signals and regulatory p...

journal_title：Journal of bacteriology

authors： Hovel-Miner G,Pampou S,Faucher SP,Clarke M,Morozova I,Morozov P,Russo JJ,Shuman HA,Kalachikov S

更新日期：2009-04-01 00:00:00

abstract：:We identified in Listeria monocytogenes a gene encoding a protein homologous to MecA, a regulatory protein acting with ClpC and ComK in the competence pathway of Bacillus subtilis. In L. monocytogenes, MecA is involved, along with ClpC and ClpP, in the downregulation of a 64-kDa secreted protein. In B. subtilis, the M...

journal_title：Journal of bacteriology

authors： Borezee E,Msadek T,Durant L,Berche P

更新日期：2000-10-01 00:00:00

abstract：:Bacillus subtilis strains containing deletions in the genes coding for one or two of the major small, acid-soluble spore proteins (SASP; termed SASP-alpha and SASP-beta) were constructed. These mutants sporulated normally, but the spores lacked either SASP-alpha, SASP-beta, or both proteins. The level of minor SASP di...

journal_title：Journal of bacteriology

authors： Mason JM,Setlow P

更新日期：1986-07-01 00:00:00

abstract：:An obligate thermophilic strain of Bacillus stearothermophilus, strain PH24, isolated from industrial sediment by elective culture, grew readily at 55 C on phenol or on one of the isomers of cresol as the major carbon source. Intact cells grown in the presence of phenol, o-cresol, m-cresol, or p-cresol were induced to...

journal_title：Journal of bacteriology

authors： Buswell JA

更新日期：1975-12-01 00:00:00

abstract：:Saz, Arthur K. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), Dolores L. Lowery, and Leah J. Jackson. Staphylococcal penicillinase. I. Inhibition and stimulation of activity. J. Bacteriol. 82:298-304. 1961.-The penicillinase extracted from a penicillin-resistant strain of Staphylococcus aureus...

journal_title：Journal of bacteriology

authors： SAZ AK,LOWERY DL,JACKSON LJ

更新日期：1961-08-01 00:00:00

abstract：:Agrobacterium radiobacter NCIB 11883 was grown in lactose-limited continuous culture at a dilution rate of 0.045/h. Washed cells transported [14C]lactose and [methyl-14C]beta-D-thiogalactoside, a nonmetabolisable analog of lactose, at similar rates and with similar affinities (Km for transport, less than 1 microM). Tr...

journal_title：Journal of bacteriology

authors： Greenwood JA,Cornish A,Jones CW

更新日期：1990-04-01 00:00:00

abstract：:The multicellular developmental cycle of Myxococcus xanthus requires large-scale changes in gene transcription, and recent findings indicate that NtrC-like activators play a prominent role in regulating these changes. In this study, we made insertions in 28 uncharacterized ntrC-like activator (nla) genes and found tha...

journal_title：Journal of bacteriology

authors： Caberoy NB,Welch RD,Jakobsen JS,Slater SC,Garza AG

更新日期：2003-10-01 00:00:00

abstract：:Bacillus brevis 47 contains two cell wall proteins termed the outer wall protein (OWP) and middle wall protein (MWP), each of which forms hexagonal arrays in the cell wall. A 6-kilobase BglII-BclI fragment of B. brevis 47 DNA cloned into Bacillus subtilis with a derivative of pHW1 as a vector directed the synthesis of...

journal_title：Journal of bacteriology

authors： Tsuboi A,Uchihi R,Tabata R,Takahashi Y,Hashiba H,Sasaki T,Yamagata H,Tsukagoshi N,Udaka S

更新日期：1986-10-01 00:00:00

abstract：:The uhp locus of Escherichia coli contains genes for the sugar phosphate transport system (uhpT) and the regulatory system which allows its induction by external glucose 6-phosphate (uhpRA). The uhp region was cloned onto high-copy-number plasmids, both from Uhp(+) plasmids of the Clarke-Carbon collection and from gen...

journal_title：Journal of bacteriology

authors： Shattuck-Eidens DM,Kadner RJ

更新日期：1983-09-01 00:00:00

abstract：:The expression of the positively charged human protein secretory leukocyte protease inhibitor (SLPI) in Escherichia coli causes severe cellular toxicity. After induction of SLPI synthesis in a high-level-expression strain, SGE61, the growth of the strain is arrested and total protein and RNA synthesis rates decline by...

journal_title：Journal of bacteriology

authors： Miller KW,Evans RJ,Eisenberg SP,Thompson RC

更新日期：1989-04-01 00:00:00

abstract：:Mutants for 9 of the 10 steps in histidine biosynthesis have been isolated and identified by enzyme assay. Each locus has been mapped in relation to the aro cluster and to other histidine loci by deoxyribonucleic acid-mediated transformation. The genes which code for enzymes 3, 6, and 8 of the pathway are linked to th...

journal_title：Journal of bacteriology

authors： Chapman LF,Nester EW

更新日期：1969-03-01 00:00:00

abstract：:Sequencing the insertion sites of 8,865 Tn917 insertions in Enterococcus faecalis strain OG1RF identified a hot spot in the replication terminus region corresponding to 6% of the genome where 65% of the transposons had inserted. In E. faecalis, Tn917 preferentially inserted at a 29-bp consensus sequence centered on TA...

journal_title：Journal of bacteriology

authors： Garsin DA,Urbach J,Huguet-Tapia JC,Peters JE,Ausubel FM

更新日期：2004-11-01 00:00:00

abstract：:Further studies on the expression of the two aspartokinase activities in Bacillus bovis are presented. Aspartokinase I (previously shown to be inhibited and repressed by lysine) was found to be repressed by diaminopimelate in the wild-type strain. However, in a mutant unable to convert diaminopimelate to lysine, starv...

journal_title：Journal of bacteriology

authors： Hitchcock MJ,Hodgson B,Linforth JL

更新日期：1980-05-01 00:00:00

abstract：:The two-component regulatory system, composed of virA and virG, is indispensable for transcription of virulence genes within Agrobacterium tumefaciens. However, virA and virG are insufficient to activate transcription from virulence gene promoters within Escherichia coli cells, indicating a requirement for additional ...

journal_title：Journal of bacteriology

authors： Lohrke SM,Nechaev S,Yang H,Severinov K,Jin SJ

更新日期：1999-08-01 00:00:00

abstract：:We purified active hydrogenase from free-living Rhizobium japonicum by affinity chromatography. The uptake hydrogenase of R. japonicum has been treated previously as an oxygen-sensitive protein. In this purification, however, reducing agents were not added nor was there any attempt to exclude oxygen. In fact, the addi...

journal_title：Journal of bacteriology

authors： Stults LW,Moshiri F,Maier RJ

更新日期：1986-06-01 00:00:00

abstract：:We have identified three transposable gene-activating elements from Pseudomonas cepacia on the basis of their abilities to increase expression of the lac genes of the broad-host-range plasmid pGC91.14 (pRP1::Tn951). When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), this plasmid failed to con...

journal_title：Journal of bacteriology

authors： Wood MS,Lory C,Lessie TG

更新日期：1990-04-01 00:00:00

abstract：:Rhodococcus sp. strain DK17 is capable of utilizing various derivatives of benzene and bicyclics containing both aromatic and alicyclic moieties as sole carbon and energy sources. Here, we present the 9,107,362-bp draft genome sequence of DK17 and its genomic analysis in comparison with other members of the genus Rhod...

journal_title：Journal of bacteriology

authors： Yoo M,Kim D,Choi KY,Chae JC,Zylstra GJ,Kim E

更新日期：2012-08-01 00:00:00

abstract：:From citrate-utilizing (Cit+) Escherichia coli strain C53 of bovine origin, strains C53A and C53B were obtained. Upon mating with recA+ but not with recA mutant recipients of K-12, C53A produced chromosomal recombinants at quite high frequencies, leading to the following conclusions: (i) C53A is an Hfr strain; (ii) th...

journal_title：Journal of bacteriology

authors： Ishiguro N,Oka C,Asagi M,Sato G,Yoshikawa M

更新日期：1980-08-01 00:00:00

abstract：:In Escherichia coli K-12, expression of the lysU gene is regulated by the lrp gene product, as indicated by an increase in the level of lysyl-tRNA synthetase activity and LysU protein in an lrp mutant. Comparison of the patterns of protein expression visualized by two-dimensional gel electrophoresis indicated that Lys...

journal_title：Journal of bacteriology

authors： Lin R,Ernsting B,Hirshfield IN,Matthews RG,Neidhardt FC,Clark RL,Newman EB

更新日期：1992-05-01 00:00:00