Direction of deoxyribonucleic acid transfer and replication in a derivative of plasmid R100-1.

abstract：:The transferred DNA (T-DNA) is transported from Agrobacterium tumefaciens to the nucleus and is stably integrated into the genome of many plant species. It has been proposed that the VirD2 protein, tightly attached to the T-DNA, pilots the T-DNA into the plant cell nucleus and that it is involved in integration. Using...

journal_title：Journal of bacteriology

authors： Koukolíková-Nicola Z,Raineri D,Stephens K,Ramos C,Tinland B,Nester EW,Hohn B

更新日期：1993-02-01 00:00:00

abstract：:Periplasmic membrane fusion proteins (MFPs) are essential components of multidrug efflux pumps and type I protein secretion systems of gram-negative bacteria. Located in the periplasm, MFPs function by creating a physical link between inner membrane transporters and outer membrane channels. The most conserved sequence...

journal_title：Journal of bacteriology

authors： Ge Q,Yamada Y,Zgurskaya H

更新日期：2009-07-01 00:00:00

abstract：:The effect of dihydrostreptomycin on the incorporation of amino acids into protein in antibiotic-deprived cells of a streptomycin-dependent strain of Escherichia coli B has been compared with its effect on protein synthesis in extracts from cells of the same strain. Stimulation of phenylalanine incorporation into prot...

journal_title：Journal of bacteriology

authors： Dixon H,Polglase WJ

更新日期：1969-10-01 00:00:00

abstract：:In order to characterize the genome-wide transcriptional response of the hyperthermophilic, aerobic crenarchaeote Sulfolobus solfataricus to UV damage, we used high-density DNA microarrays which covered 3,368 genetic features encoded on the host genome, as well as the genes of several extrachromosomal genetic elements...

journal_title：Journal of bacteriology

authors： Fröls S,Gordon PM,Panlilio MA,Duggin IG,Bell SD,Sensen CW,Schleper C

更新日期：2007-12-01 00:00:00

abstract：:Utilizing a homologous gene replacement in order to retain the native promoter and 5' and 3' untranslated messenger regions (and thereby ensure physiological validity), we have shown that deletion of the N-terminal 389 amino acids of the transcriptional activator AREA does not result in nitrogen metabolite derepressio...

journal_title：Journal of bacteriology

authors： Caddick MX,Arst HN Jr

更新日期：1998-11-01 00:00:00

abstract：:Manganese is accumulated in Bacillus subtilis by a highly specific active transport system. This trace element "pump" is insensitive to added magnesium or calcium and preferentially accumulates manganese in the presence of cobalt, iron, and copper. Manganese uptake in B. subtilis is inhibited by cyanide, azide, pentac...

journal_title：Journal of bacteriology

authors： Eisenstadt E,Fisher S,Der CL,Silver S

更新日期：1973-03-01 00:00:00

abstract：:Escherichia coli K-12 strain CU1008 cannot use L-serine as the sole carbon source, but it could use L-serine as an auxiliary carbon source with glucose, L-alanine, or pyruvate and could derive energy from L-serine to support oxygen uptake. CU1008 grew with L-serine if it was also provided with glycine and leucine. The...

journal_title：Journal of bacteriology

authors： Newman EB,Walker C

更新日期：1982-08-01 00:00:00

abstract：:Cystic fibrosis (CF) patients are highly susceptible to chronic pulmonary disease caused by mucoid Pseudomonas aeruginosa strains that overproduce the exopolysaccharide alginate. We showed here that a mutation in zwf, encoding glucose-6-phosphate dehydrogenase (G6PDH), leads to a approximately 90% reduction in alginat...

journal_title：Journal of bacteriology

authors： Silo-Suh L,Suh SJ,Phibbs PV,Ohman DE

更新日期：2005-11-01 00:00:00

abstract：:Metal-dependent superoxide dismutases (SODs) with a specific requirement for a manganese or iron ion for catalytic activity and copper- and zinc-dependent enzymes are essential for detoxification of superoxide anion radicals. Genome sequence analyses predict the existence of a nickel-dependent enzyme (NiSOD) as the un...

journal_title：Journal of bacteriology

authors： Eitinger T

更新日期：2004-11-01 00:00:00

abstract：:Anaerobic growth of Klebsiella aerogenes NCDO 711 (NCTC 418) on citrate was dependent on the presence of Na+ in the medium, and fermentation of citrate was mediated via the fermentation pathway enzymes, citrate lyase and a Na+-dependent oxalacetate decarboxylase. This confirms the previous findings on strain NCTC 418....

journal_title：Journal of bacteriology

authors： O'Brien RW

更新日期：1975-05-01 00:00:00

abstract：:Microorganisms that use sulfate as a terminal electron acceptor for anaerobic respiration play a central role in the global sulfur cycle. Here, we report the results of comparative sequence analysis of dissimilatory sulfite reductase (DSR) genes from closely and distantly related sulfate-reducing organisms to infer th...

journal_title：Journal of bacteriology

authors： Wagner M,Roger AJ,Flax JL,Brusseau GA,Stahl DA

更新日期：1998-06-01 00:00:00

abstract：:Mild alkaline extraction of native poly-beta-hydroxybutyrate (PHB) granules results in the solubilization of a protein fraction. Both the solubilized protein fraction and the extracted granules are essentially devoid of PHB synthetase activity unless recombined. The protein fraction has been separated by chromatograph...

journal_title：Journal of bacteriology

authors： Griebel RJ,Merrick JM

更新日期：1971-11-01 00:00:00

abstract：:Kobayashi, George S. (Tulane University, New Orleans, La.), Lorraine Friedman, and Judith F. Kofroth. Some cytological and pathogenic properties of spheroplasts of Candida albicans. J. Bacteriol. 88:795-801. 1964.-Spheroplasts of Candida albicans were prepared by use of an enzymatic mixture from the digestive tract of...

journal_title：Journal of bacteriology

authors： KOBAYASHI GS,FRIEDMAN L,KOFROTH JF

更新日期：1964-09-01 00:00:00

abstract：:Thermococcus sp. strain 4557 is a hyperthermophilic anaerobic archaeon isolated from the deep-sea hydrothermal vent Guaymas Basin site in the Gulf of California at a depth of 2,000 m. Here, we present the complete genome sequence of Thermococcus sp. 4557, which consists of a single circular chromosome of 2,011,320 bp ...

journal_title：Journal of bacteriology

authors： Wang X,Gao Z,Xu X,Ruan L

更新日期：2011-10-01 00:00:00

abstract：:We have developed a general method for determining whether a gene of Escherichia coli is essential for viability. The method requires cloned DNA spanning the gene in question and a reasonably detailed genetic and physical map of the cloned segment. Using this information, one constructs a deletion of the target gene i...

journal_title：Journal of bacteriology

authors： Joyce CM,Grindley ND

更新日期：1984-05-01 00:00:00

abstract：:Type 1 pili of Escherichia coli are the prototype of the somatic class of pili found on many strains of bacteria. As a first step in the genetic analysis of type 1 piliation, an extensive series of nonpiliated derivatives of E. coli K-12 strain AW405, was characterized to produce attached or free pili when examined in...

journal_title：Journal of bacteriology

authors： Swaney LM,Liu YP,To CM,To CC,Ippen-Ihler K,Brinton CC Jr

更新日期：1977-04-01 00:00:00

abstract：:A computer program has been designed to study behavior in populations of Spirochaeta aurantia cells, and this program has been used to analyze changes in behavior in response to chemoattractants. Three kinds of behavior were distinguished: smooth swimming, flexing, and reversals in direction of swimming after a short ...

journal_title：Journal of bacteriology

authors： Fosnaugh K,Greenberg EP

更新日期：1988-04-01 00:00:00

abstract：:In Caulobacter crescentus, progression through the cell cycle is governed by the periodic activation and inactivation of the master regulator CtrA. Two phosphorelays, each initiating with the histidine kinase CckA, promote CtrA activation by driving its phosphorylation and by inactivating its proteolysis. Here, we exa...

journal_title：Journal of bacteriology

authors： Chen YE,Tsokos CG,Biondi EG,Perchuk BS,Laub MT

更新日期：2009-12-01 00:00:00

abstract：:The mechanisms by which hyperthermophilic Archaea, such as "Pyrococcus abyssi" and Pyrococcus furiosus, survive high doses of ionizing gamma irradiation are not thoroughly elucidated. Following gamma-ray irradiation at 2,500 Gy, the restoration of "P. abyssi" chromosomes took place within chromosome fragmentation. DNA...

journal_title：Journal of bacteriology

authors： Jolivet E,Matsunaga F,Ishino Y,Forterre P,Prieur D,Myllykallio H

更新日期：2003-07-01 00:00:00

abstract：:The structure and composition of the cell walls of hyphae of Neurospora crassa were investigated by electron microscopy, chemical analysis, and X-ray diffraction both before and after progressive enzymatic degradation by snail gut enzymes, chitinase, and trypsin. The wall consists of two phases: randomly disposed skel...

journal_title：Journal of bacteriology

authors： Manocha MS,Colvin JR

更新日期：1967-07-01 00:00:00

abstract：:The Staphylococcus aureus autoinducer-2 (AI-2) producer protein LuxS is phosphorylated by the Ser/Thr kinase Stk1 at a unique position, Thr14. The enzymatic activity of the phosphorylated isoform of LuxS was abrogated compared to that of nonphosphorylated LuxS, thus providing the first evidence of an AI-2-producing en...

journal_title：Journal of bacteriology

authors： Cluzel ME,Zanella-Cléon I,Cozzone AJ,Fütterer K,Duclos B,Molle V

更新日期：2010-12-01 00:00:00

abstract：:The 3.1-Mb genome of an outbreak methicillin-resistant Staphylococcus aureus (MRSA) strain (TW20) contains evidence of recently acquired DNA, including two large regions (635 kb and 127 kb). The strain is resistant to a wide range of antibiotics, antiseptics, and heavy metals due to resistance genes encoded on mobile ...

journal_title：Journal of bacteriology

authors： Holden MT,Lindsay JA,Corton C,Quail MA,Cockfield JD,Pathak S,Batra R,Parkhill J,Bentley SD,Edgeworth JD

更新日期：2010-02-01 00:00:00

abstract：:Triggering of germination in Bacillus megaterium QM B1551 spores with D-glucose was studied. First, the interaction of glucose with spores for less than 1 min resulted in triggering almost 90% of the spores after the glucose was removed by dilution. Therefore only a brief time is needed for glucose to trigger germinat...

journal_title：Journal of bacteriology

authors： Racine FM,Dills SS,Vary JC

更新日期：1979-05-01 00:00:00

abstract：:A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) is essential for the initiation of aerial mycelium formation in Streptomyces griseus. amfR is one of the genes which, when cloned on a low-copy-number plasmid, suppresses the aerial mycelium-negative phenotype of an A-factor-deficient mutant of S. griseus....

journal_title：Journal of bacteriology

authors： Ueda K,Hsheh CW,Tosaki T,Shinkawa H,Beppu T,Horinouchi S

更新日期：1998-10-01 00:00:00

abstract：:The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made an...

journal_title：Journal of bacteriology

authors： Scheffers DJ,Robichon C,Haan GJ,den Blaauwen T,Koningstein G,van Bloois E,Beckwith J,Luirink J

更新日期：2007-10-01 00:00:00

abstract：:In Escherichia coli, synthesis of sialic acid is not regulated by allosteric inhibition mediated by cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). Evidence for the lack of metabolic control by feedback inhibition was demonstrated by measuring the intracellular level of sialic acid and CMP-NeuNAc in muta...

journal_title：Journal of bacteriology

authors： Vimr ER,Troy FA

更新日期：1985-11-01 00:00:00

abstract：:The recA gene from Thermus thermophilus HB27 was cloned and engineered to obtain insertion (recA::kat) and deletion (deltarecA) derivatives. Transcription of recA in this extreme thermophile was induced by mitomycin C, leading to the synthesis of a monocistronic mRNA. This DNA damage-mediated induction was dependent o...

journal_title：Journal of bacteriology

authors： Castán P,Casares L,Barbé J,Berenguer J

更新日期：2003-08-01 00:00:00

abstract：:We have cloned and sequenced a cluster of six open reading frames containing gene kdsA from Escherichia coli K-12. The gene encodes 3-deoxy-D-manno-octulosonate 8-phosphate synthetase (KDO-8-phosphate synthetase), which catalyzes formation of 3-deoxy-D-manno-octulosonic acid (KDO), an essential component of enterobact...

journal_title：Journal of bacteriology

authors： Strohmaier H,Remler P,Renner W,Högenauer G

更新日期：1995-08-01 00:00:00

abstract：:The host factor (HF-I) for phage Q beta RNA replication is a small protein of 102 amino acid residues encoded by the hfq gene at 94.8 min on the Escherichia coli chromosome. The synthesis rate of HF-I at the exponential-growth phase is higher than at the stationary phase, and it increases concomitantly with the increa...

journal_title：Journal of bacteriology

authors： Kajitani M,Kato A,Wada A,Inokuchi Y,Ishihama A

更新日期：1994-01-01 00:00:00

abstract：:Cecropins are a family of small, basic antibacterial polypeptides which can be isolated from pupae of immunized Lepidoptera. They are active against both gram-negative and gram-positive bacteria. We studied a mutant of Escherichia coli, strain SB1004, which is more sensitive to cecropin D than is the parental strain. ...

journal_title：Journal of bacteriology

authors： Sidén I,Boman HG

更新日期：1983-04-01 00:00:00