Physiological responses of the hyperthermophilic archaeon "Pyrococcus abyssi" to DNA damage caused by ionizing radiation.

Abstract:

:The mechanisms by which hyperthermophilic Archaea, such as "Pyrococcus abyssi" and Pyrococcus furiosus, survive high doses of ionizing gamma irradiation are not thoroughly elucidated. Following gamma-ray irradiation at 2,500 Gy, the restoration of "P. abyssi" chromosomes took place within chromosome fragmentation. DNA synthesis in irradiated "P. abyssi" cells during the DNA repair phase was inhibited in comparison to nonirradiated control cultures, suggesting that DNA damage causes a replication block in this organism. We also found evidence for transient export of damaged DNA out of irradiated "P. abyssi" cells prior to a restart of chromosomal DNA synthesis. Our cell fractionation assays further suggest that "P. abyssi" contains a highly efficient DNA repair system which is continuously ready to repair the DNA damage caused by high temperature and/or ionizing radiation.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Jolivet E,Matsunaga F,Ishino Y,Forterre P,Prieur D,Myllykallio H

doi

10.1128/jb.185.13.3958-3961.2003

keywords:

subject

Has Abstract

pub_date

2003-07-01 00:00:00

pages

3958-61

issue

13

eissn

0021-9193

issn

1098-5530

journal_volume

185

pub_type

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