Abstract:
:Pseudomonas sp. strain ACP is capable of growth on 1-aminocyclopropane-1-carboxylate (ACC) as a nitrogen source owing to induction of the enzyme ACC deaminase and the subsequent conversion of ACC to alpha-ketobutyrate and ammonia (M. Honma, Agric. Biol. Chem. 49:567-571, 1985). The complete amino acid sequence of purified ACC deaminase was determined, and the sequence information was used to clone the ACC deaminase gene from a 6-kb EcoRI fragment of Pseudomonas sp. strain ACP DNA. DNA sequence analysis of an EcoRI-PstI subclone demonstrated an open reading frame (ORF) encoding a polypeptide with a deduced amino acid sequence identical to the protein sequence determined chemically and a predicted molecular mass of 36,674 Da. The ORF also contained an additional 72 bp of upstream sequence not predicted by the amino acid sequence. Escherichia coli minicells containing the 6-kb clone expressed a major polypeptide of the size expected for ACC deaminase which was reactive with ACC deaminase antiserum. Furthermore, a lacZ fusion with the ACC deaminase ORF resulted in the expression of active enzyme in E. coli. ACC is a key intermediate in the biosynthesis of ethylene in plants, and the use of the ACC deaminase gene to manipulate this pathway is discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Sheehy RE,Honma M,Yamada M,Sasaki T,Martineau B,Hiatt WRdoi
10.1128/jb.173.17.5260-5265.1991subject
Has Abstractpub_date
1991-09-01 00:00:00pages
5260-5issue
17eissn
0021-9193issn
1098-5530journal_volume
173pub_type
杂志文章abstract::Escherichia coli AT2046 has been shown previously to lack the enzyme serine transhydroxymethylase and to require exogenous glycine for growth as a consequence. Strains JEV73 and JEV73R, mutants derived from strain AT2046, are shown here to be serine transhydroxymethylase deficient, but able to derive their glycine fro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.3.810-817.1975
更新日期:1975-06-01 00:00:00
abstract::The invasion of intestinal epithelial cells by Salmonella enterica serovar Typhimurium is mediated by a type III secretion system (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). Expression of the SPI1 T3SS is tightly regulated by the combined action of HilC, HilD, and RtsA, three AraC family members that c...
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journal_title:Journal of bacteriology
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doi:10.1128/JB.158.2.551-561.1984
更新日期:1984-05-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.19.5904-5911.1994
更新日期:1994-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.1.107-114.1979
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pub_type: 杂志文章
doi:10.1128/jb.167.1.350-355.1986
更新日期:1986-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.1.107-112.1969
更新日期:1969-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2019-07-24 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.10.2975-2977.1996
更新日期:1996-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.1.167-177.1973
更新日期:1973-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.2.727-734.1979
更新日期:1979-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.1.1-3.1977
更新日期:1977-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.1154-1155.1977
更新日期:1977-02-01 00:00:00
abstract::The morphology, on MacConkey's agar, of colonies of the bacteria commonly found in enteric specimens is sufficiently characteristic and distinctive, when observed with transmitted oblique illumination and a low-power stereoscopic microscope, to enable identification at the generic (and occasionally the species) level ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.6.1897-1905.1967
更新日期:1967-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.186.10.3182-3186.2004
更新日期:2004-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.22.6623-6627.1996
更新日期:1996-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.12.3536-3543.1994
更新日期:1994-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.1.231-237.1980
更新日期:1980-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.1.131-142.1969
更新日期:1969-04-01 00:00:00
abstract::The effects of high doses of radiation (1, 5, or 20 Mrad) on the toxicity, pyrogenicity, and immunogenicity of Salmonella typhimurium cells and endotoxin were studied. Toxicity decreased progressively after exposure to 1, 5, or 20 Mrad. The lethal effect of 1-Mrad exposed cells was greater than that of heat-, acetone-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.6.2165-2170.1968
更新日期:1968-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.21.6301-6303.1995
更新日期:1995-11-01 00:00:00
abstract::Bacillus subtilis strains carrying div-341 or sacU mutations, or both, have been characterized to reveal the roles of both genes in the initiation of sporulation, as well as in cell division and exoenzyme secretion. Both mutations were closely linked by transformation and caused the pleiotropic effects on sporulation ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.2.648-653.1985
更新日期:1985-08-01 00:00:00
abstract::When the stop codons TGA, TAA, and TAG are found in the second and third reading frames of a protein-encoding gene, they are considered premature stop codons (PSC). Deinococcus radiodurans disproportionately favored TGA more than the other two triplets as a PSC. The TGA triplet was also found more often in noncoding r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-10-01 00:00:00
abstract::Pigmentation mutants of Pseudomonas aeruginosa, selected by observed visual differences in coloration from the wild-type strain, were examined for altered patterns of phenazine synthesis. Three classes of mutants that were incapable of pyocyanine production were identified. Pigmentation patterns that were found to cha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.3.846-852.1979
更新日期:1979-06-01 00:00:00
abstract::The marRAB operon is one of two operons in the mar locus of Escherichia coli that are divergently transcribed from a central regulatory region, marO. The marRAB operon, transcribed from marOII, controls intrinsic resistance or susceptibility to multiple antibiotics and is inducible by structurally unrelated compounds ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.12.3414-3419.1995
更新日期:1995-06-01 00:00:00
abstract::The 2,760-base-pair (bp) PstI-EcoRI segment of the chromosome of Pseudomonas aeruginosa PA103 which carries the exotoxin A structural gene was expressed from an internal promoter when cloned in a pUC9 derivative and transformed into a nontoxigenic mutant of P. aeruginosa PAO1. The unique terminal EcoRI site was delete...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2599-2604.1989
更新日期:1989-05-01 00:00:00
abstract::Aspergillus fumigatus, an important opportunistic pathogen which commonly affects neutropenic patients, produces conidia with a bluish-green color. We identified a gene, alb1, which is required for conidial pigmentation. The alb1 gene encodes a putative polyketide synthase, and disruption of alb1 resulted in an albino...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.12.3031-3038.1998
更新日期:1998-06-01 00:00:00