Cnu, a novel oriC-binding protein of Escherichia coli.

abstract：:Although four of the five Salmonella pathogenicity islands (SPIs) have been characterized in detail for Salmonella enterica serovar Typhimurium, and the fifth has been characterized for Salmonella enterica serovar Dublin, there have been limited studies to examine them in detail in a range of pathogenic serovars of S....

journal_title：Journal of bacteriology

authors： Amavisit P,Lightfoot D,Browning GF,Markham PF

更新日期：2003-06-01 00:00:00

abstract：:Anabaena variabilis ATCC 29413 is a heterotrophic, nitrogen-fixing cyanobacterium that has been reported to fix nitrogen and reduce acetylene to ethane in the absence of molybdenum. DNA from this strain hybridized well at low stringency to the nitrogenase 2 (vnfDGK) genes of Azotobacter vinelandii. The hybridizing reg...

journal_title：Journal of bacteriology

authors： Thiel T

更新日期：1993-10-01 00:00:00

abstract：:Outer membranes, free of cytoplasmic or thylakoid membranes and peptidoglycan components, were obtained from Synechocystis sp. strain PCC6714. Electron microscope studies revealed double-track outer membrane vesicles with a smooth-appearing exoplasmic surface, an exoplasmic fracture face covered by closely packed part...

journal_title：Journal of bacteriology

authors： Jürgens UJ,Weckesser J

更新日期：1985-10-01 00:00:00

abstract：:In Escherichia coli, T:G mismatches in specific contexts are corrected by a very short patch (VSP) repair system. Previous studies have shown that the product of gene vsr mediates correction of T:G to C:G in the 5'CTAGG/3'GGTCC context and in some related contexts. Amber mutations that arose in CAG sequences in gene c...

journal_title：Journal of bacteriology

authors： Lieb M,Rehmat S

更新日期：1995-02-01 00:00:00

abstract：:Two nrdF genes, nrdF1 and nrdF2, encoding the small subunit (R2) of ribonucleotide reductase (RR) from Mycobacterium tuberculosis have 71% identity at the amino acid level and are both highly homologous with Salmonella typhimurium R2F. The calculated molecular masses of R2-1 and R2-2 are 36,588 (322 amino acids [aa]) ...

journal_title：Journal of bacteriology

authors： Yang F,Curran SC,Li LS,Avarbock D,Graf JD,Chua MM,Lu G,Salem J,Rubin H

更新日期：1997-10-01 00:00:00

abstract：:RsbT is an essential component of the pathway that activates the Bacillus subtilis sigma(B) transcription factor in response to physical stress. rsbT is located within an operon that includes the genes for its principal negative regulator (RsbS) and the stress pathway component that it activates (RsbU), as immediate u...

journal_title：Journal of bacteriology

authors： Zhang S,Reeves A,Woodbury RL,Haldenwang WG

更新日期：2005-12-01 00:00:00

abstract：:Tracer studies of pyrimidine biosynthesis in Lactobacillus leichmannii (ATCC 7830) indicated that, while aspartate is utilized in the usual manner, the guanido carbon of arginine, rather than carbon dioxide, is utilized as a pyrimidine precursor. The guanido carbon of arginine also contributes, to some extent, to the ...

journal_title：Journal of bacteriology

authors： Hutson JY,Downing M

更新日期：1968-10-01 00:00:00

abstract：:The citrate utilization (Cit+) transposon Tn3411 was shown to be flanked by directly repeated sequences (IS3411L and IS3411R) by restriction enzyme analysis and electron microscope observation. Cit- deletion mutants were frequently found to be generated in pBR322::Tn3411 by intramolecular recombination between the two...

journal_title：Journal of bacteriology

authors： Ishiguro N,Sato G

更新日期：1984-11-01 00:00:00

abstract：:Agrobacterium rhizogenes strain 15834, which incites hairy root disease in plants, harbors three large plasmids: pAr15834a (107 x 10(6) daltons), pAr15834b (154 x 10(6) daltons), and pAr15834c (258 x 10(6) daltons). Kanamycin-resistant transconjugants were selected in a cross of kanamycin-resistant derivate of strain ...

journal_title：Journal of bacteriology

authors： White FF,Nester EW

更新日期：1980-03-01 00:00:00

abstract：:The genome of the Philadelphia-1 strain of Legionella pneumophila, the causative organism of Legionnaires' disease, encodes two virulence-associated type 4 secretion systems (T4SSs), the Dot/Icm type 4B (T4BSS) and the Lvh type 4A (T4ASS). Broth stationary-phase cultures of most dot/icm mutants are defective in entry ...

journal_title：Journal of bacteriology

authors： Bandyopadhyay P,Lang EA,Rasaputra KS,Steinman HM

更新日期：2013-08-01 00:00:00

abstract：:Linker insertion mutagenesis was used to modify the paracrystalline surface layer (S-layer) protein (RsaA) of the gram-negative bacterium Caulobacter crescentus. Eleven unique BamHI linker insertions in the cloned rsaA gene were identified; at the protein level, these linker insertions introduced 4 to 6 amino acids at...

journal_title：Journal of bacteriology

authors： Bingle WH,Nomellini JF,Smit J

更新日期：1997-02-01 00:00:00

abstract：:The phs mutation, which causes a pleiotropic growth defect, has been mapped and shown to be an allele of rpoA, the gene for the alpha subunit of RNA polymerase. The mutation is shown to cause a transcription defect in the arabinose operon, araBAD. ...

journal_title：Journal of bacteriology

authors： Rowland GC,Giffard PM,Booth IR

更新日期：1985-11-01 00:00:00

abstract：:Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunological techniques, we have compared the synthesis of the phoA protein (alkaline phosphatase) and the phoS protein (phosphate-binding protein) in response to the level of phosphate in the medium in different genetic backgrounds containing the kn...

journal_title：Journal of bacteriology

authors： Willsky GR,Malamy MH

更新日期：1976-07-01 00:00:00

abstract：:The copy number of 2 mu DNA-derived plasmids in CIR+ Saccharomyces cerevisiae transformants is determined by its selective marker and is usually much lower than that of the endogenous plasmid. Only plasmids containing the leu2 allele of pJDB219, designated as leu2-d, under selective conditions displayed a higher copy ...

journal_title：Journal of bacteriology

authors： Erhart E,Hollenberg CP

更新日期：1983-11-01 00:00:00

abstract：:Rubrerythrin was purified by multistep chromatography under anaerobic, reducing conditions from the hyperthermophilic archaeon Pyrococcus furiosus. It is a homodimer with a molecular mass of 39.2 kDa and contains 2.9 +/- 0.2 iron atoms per subunit. The purified protein had peroxidase activity at 85 degrees C using hyd...

journal_title：Journal of bacteriology

authors： Weinberg MV,Jenney FE Jr,Cui X,Adams MW

更新日期：2004-12-01 00:00:00

abstract：:The role of the proton gradient and external pH in the motility and chemotaxis of Bacillus subtilis was investigated. Presence of a substantial proton gradient is not necessary for motility or chemotaxis, as long as the electrical potential is sufficient to maintain motility. Changes in the proton gradient do, however...

journal_title：Journal of bacteriology

authors： Miller JB,Koshland DE Jr

更新日期：1980-01-01 00:00:00

abstract：:The xylanase XynC of Fibrobacter succinogenes S85 was recently shown to contain three distinct domains, A, B, and C (F. W. Paradis, H. Zhu, P. J. Krell, J. P. Phillips, and C. W. Forsberg, J. Bacteriol. 175:7666-7672, 1993). Domains A and B each bear an active site capable of hydrolyzing xylan, while domain C has no e...

journal_title：Journal of bacteriology

authors： Zhu H,Paradis FW,Krell PJ,Phillips JP,Forsberg CW

更新日期：1994-07-01 00:00:00

abstract：:Leucine and isoleucine-valine loci have been mapped in Rhizobium japonicum. Transformation analysis suggests a common pathway for isoleucine-valine biosynthesis. Three-point reciprocal crosses indicated that all the leucine loci are not genetically linked. ...

journal_title：Journal of bacteriology

authors： Doctor F,Modi VV

更新日期：1976-05-01 00:00:00

abstract：:A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogen...

journal_title：Journal of bacteriology

authors： Von Tersch MA,Carlton BC

更新日期：1984-12-01 00:00:00

abstract：:Klite, P. D. (Middle America Research Unit, Balboa Heights, Canal Zone). Intestinal bacterial flora and transit time of three neotropical bat species. J. Bacteriol. 90:375-379. 1965.-Quantitative studies on the intestinal bacterial flora of three neotropical bat species revealed the following average bacterial populat...

journal_title：Journal of bacteriology

authors： KLITE PD

更新日期：1965-08-01 00:00:00

abstract：:The presence of acetate and pyruvate groups in Klebsiella capsular polysaccharides may be demonstrated and estimated quantitatively by running the proton magnetic resonance spectrum of the polysaccharide (as sodium salt) in deuterium oxide at 95 C. Such spectra also permit an assessment to be made of the number of alp...

journal_title：Journal of bacteriology

authors： Bebault GM,Choy YM,Dutton GG,Funnell N,Stephen AM,Yang MT

更新日期：1973-03-01 00:00:00

abstract：:We measured some of the kinetic parameters of nitrogenase to intact systems of Clostridium pasteurianum and Klebsiella pneumoniae to compare them with the kinetics of the enzyme in vitro. We found that the enzyme showed multiple apparent Km values for acetylene reduction in vivo, as it does in vitro. Carbon monoxide w...

journal_title：Journal of bacteriology

authors： Davis LC,Wang YL

更新日期：1980-03-01 00:00:00

abstract：:A strain of Escherichia coli which was derived from a gentamicin-resistant clinical isolate was found to be cross-resistant to neomycin and streptomycin. The molecular nature of the genetic defect was found to be an insertion of two GC base pairs in the uncG gene of the mutant. The insertion led to the production of a...

journal_title：Journal of bacteriology

authors： Humbert R,Altendorf K

更新日期：1989-03-01 00:00:00

abstract：:In a horse serum-based medium containing a full complement of fatty acids, cells of Spiroplasma citri were seen to preferentially incorporate palmitic acid. In the same medium, which had a steryl ester-to-sterol ratio of 3.64, a steryl ester-to-sterol ratio of 0.23 was seen in the cells, cholesterol being preferential...

journal_title：Journal of bacteriology

authors： Freeman BA,Sissenstein R,McManus TT,Woodward JE,Lee IM,Mudd JB

更新日期：1976-03-01 00:00:00

abstract：:Hauge, Jens G. (National Institute for Public Health, Oslo, Norway). Glucose dehydrogenation in bacteria: a comparative study. J. Bacteriol. 82:609-614. 1961.-Extracts of a series of bacteria reported to convert glucose to gluconic acid via particulate enzyme systems were fractionated by differential centrifugation in...

journal_title：Journal of bacteriology

authors： HAUGE JG

更新日期：1961-10-01 00:00:00

abstract：UNLABELLED:Cell shape has been suggested to play an important role in the regulation of bacterial attachment to surfaces and the formation of communities associated with surfaces. We found that a cardiolipin synthase (Δcls) mutant of the rod-shaped bacterium Rhodobacter sphaeroides--in which synthesis of the anionic, h...

journal_title：Journal of bacteriology

authors： Lin TY,Santos TM,Kontur WS,Donohue TJ,Weibel DB

更新日期：2015-11-01 00:00:00

abstract：:Isolated sheaths of Leptothrix discophora SP-6 (ATCC 51168) were tested for susceptibility to degradation by a variety of chemical denaturants and lytic enzymes and found to be resistant to many reagents and enzyme treatments. However, disulfide bond-reducing agents such as dithiothreitol (DTT), beta-mercaptoethanol, ...

journal_title：Journal of bacteriology

authors： Emerson D,Ghiorse WC

更新日期：1993-12-01 00:00:00

abstract：:An exo-beta-1,4-glucanase (Exo A) from Ruminococcus flavefaciens FD-1 was purified to homogeneity and characterized. Enzyme activity was monitored during purification by using the substrate p-nitrophenyl-beta-D-cellobioside (NPC). Over 85% of the NPC activity was found to be extracellular once the filter paper was deg...

journal_title：Journal of bacteriology

authors： Gardner RM,Doerner KC,White BA

更新日期：1987-10-01 00:00:00

abstract：:Using PCR to create a probe based on conserved region 2 of sigma factors, we have cloned the sigA gene coding for the major sigma factor of Rhizobium meliloti. The 684-residue protein encoded by the sigA gene was expressed in vitro in coupled transcription-translation experiments with R. meliloti extracts and migrated...

journal_title：Journal of bacteriology

authors： Rushing BG,Long SR

更新日期：1995-12-01 00:00:00

abstract：:AppppA and the DnaK protein have both been hypothesized to function in regulating the heat shock response of Escherichia coli. The proposals are that AppppA serves as a signal (alarmone) to turn on the heat shock response, whereas the DnaK protein is necessary to turn off the heat shock response. A simple model would ...

journal_title：Journal of bacteriology

authors： Bochner BR,Zylicz M,Georgopoulos C

更新日期：1986-11-01 00:00:00