Outer membrane of Pseudomonas aeruginosa: heat- 2-mercaptoethanol-modifiable proteins.

Abstract:

:A number of polyacrylamide gel systems and solubilization procedures were studied to define the number and nature of "major" polypeptide bands in the outer membrane of Pseudomonas aeruginosa. It was shown that five of the eight major outer membrane proteins were "heat modifiable" in that their mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis was determined by the solubilization temperature. Four of these heat-modifiable proteins had characteristics similar to protein II of the Escherichia coli outer membrane. Addition of lipopolysaccharide subsequent to solubilization caused reversal of the heat modification. The other heat-modifiable protein, the porin protein F, was unusually stable to sodium dodecyl sulfate. Long periods of boiling in sodium dodecyl sulfate were required to cause conversion to the heat-modified form. This was demonstrated both with outer membrane-associated and purified lipopolysaccharide-depleted protein F. Furthermore, lipopolysaccharide treatment had no effect on the mobility of heat-modified protein F. Thus it is concluded that protein F represents a new class of heat-modifiable protein. It was further demonstrated that the electrophoretic mobility of protein F was modified by 2-mercaptoethanol and that the 2-mercaptoethanol and heat modification of mobility were independent of one another. The optimal conditions for the examination of the outer membrane proteins of P. aeruginosa by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis are discussed.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Hancock RE,Carey AM

doi

10.1128/JB.140.3.902-910.1979

keywords:

subject

Has Abstract

pub_date

1979-12-01 00:00:00

pages

902-10

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

140

pub_type

杂志文章
  • Complete genome sequence of Lactobacillus helveticus R0052, a commercial probiotic strain.

    abstract::Lactobacillus helveticus R0052 is a commercially available strain that is widely used in probiotic preparations. The genome sequence consisted of 2,129,425 bases. Comparative analysis showed that it was unique among L. helveticus strains in that it contained genes encoding mucus-binding proteins similar to those found...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01638-12

    authors: Tompkins TA,Barreau G,Broadbent JR

    更新日期:2012-11-01 00:00:00

  • Variations of Candida albicans electrophoretic karyotypes.

    abstract::We previously described 14 rare spontaneous morphological mutants of Candida albicans that were associated with chromosomal aberrations (E. P. Rustchenko-Bulgac, F. Sherman, and J. B. Hicks, J. Bacteriol. 172:1276-1283, 1990). Improved conditions for separation of chromosomes, as well as hybridization probes, were use...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.20.6586-6596.1991

    authors: Rustchenko-Bulgac EP

    更新日期:1991-10-01 00:00:00

  • Genome sequence of the Spinosyns-producing bacterium Saccharopolyspora spinosa NRRL 18395.

    abstract::Saccharopolyspora spinosa is a Gram-positive bacterium that produces spinosad, a well-known biodegradable insecticide that is used for agricultural pest control and has an excellent environmental and mammalian toxicological profile. Here, we present the first draft genome sequence of the type strain Saccharopolyspora ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00344-11

    authors: Pan Y,Yang X,Li J,Zhang R,Hu Y,Zhou Y,Wang J,Zhu B

    更新日期:2011-06-01 00:00:00

  • Identification in methicillin-susceptible Staphylococcus hominis of an active primordial mobile genetic element for the staphylococcal cassette chromosome mec of methicillin-resistant Staphylococcus aureus.

    abstract::We previously reported that the methicillin resistance gene mecA is carried by a novel type of mobile genetic element, SCCmec (staphylococcal cassette chromosome mec), in the chromosome of methicillin-resistant Staphylococcus aureus (MRSA). These elements are precisely excised from the chromosome and integrated into a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.9.2711-2722.2003

    authors: Katayama Y,Takeuchi F,Ito T,Ma XX,Ui-Mizutani Y,Kobayashi I,Hiramatsu K

    更新日期:2003-05-01 00:00:00

  • Role of ubiquinone in hydrogen-dependent electron transport in Rhizobium japonicum.

    abstract::Direct evidence for the involvement of ubiquinone in H2 oxidation by Rhizobium japonicum was demonstrated; H2 reduced ca. 80% of the extractable ubiquinone. The inhibitor 2-n-heptyl-4-hydroxyquinoline-N-oxide blocked electron transport at a site between ubiquinone and the cytochromes. The results showed that no cytoch...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.2.775-777.1985

    authors: O'Brian MR,Maier RJ

    更新日期:1985-02-01 00:00:00

  • A slow-motility phenotype caused by substitutions at residue Asp31 in the PomA channel component of a sodium-driven flagellar motor.

    abstract::PomA is thought to be a component of the ion channel in the sodium-driven polar-flagellar motor of Vibrio alginolyticus. We have found that some cysteine substitutions in the periplasmic region of PomA result in a slow-motility phenotype, in which swarming and swimming speeds are reduced even in the presence of high c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.11.3314-3318.2000

    authors: Kojima S,Shoji T,Asai Y,Kawagishi I,Homma M

    更新日期:2000-06-01 00:00:00

  • Characterization of the Caulobacter crescentus holdfast polysaccharide biosynthesis pathway reveals significant redundancy in the initiating glycosyltransferase and polymerase steps.

    abstract::Caulobacter crescentus cells adhere to surfaces by using an extremely strong polar adhesin called the holdfast. The polysaccharide component of the holdfast is comprised in part of oligomers of N-acetylglucosamine. The genes involved in the export of the holdfast polysaccharide and the anchoring of the holdfast to the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01003-08

    authors: Toh E,Kurtz HD Jr,Brun YV

    更新日期:2008-11-01 00:00:00

  • A genetic mechanism for deletion of the ser2 gene cluster and formation of rough morphological variants of Mycobacterium avium.

    abstract::A major phenotypic trait of the Mycobacterium avium complex is the ability to produce rough and smooth colony variants. The chemical basis of this morphological variation is the loss of an antigenic surface structure, termed glycopeptidolipid (GPL), by rough variants. Using M. avium serovar 2 strain 2151 as a model sy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.21.6177-6182.2000

    authors: Eckstein TM,Inamine JM,Lambert ML,Belisle JT

    更新日期:2000-11-01 00:00:00

  • Lactate efflux-induced electrical potential in membrane vesicles of Streptococcus cremoris.

    abstract::We developed a procedure for isolating membrane vesicles from the homolactic fermentative bacterium Streptococcus cremoris. The membrane vesicles were shown to have a right-side-out orientation by freeze-etch electron microscopy and to be free of cytoplasmic constituents. The membrane vesicles retained their functiona...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.2.733-738.1982

    authors: Otto R,Lageveen RG,Veldkamp H,Konings WN

    更新日期:1982-02-01 00:00:00

  • Stability of ribosomes and ribosomal ribonucleic acid from Bacillus stearothermophilus.

    abstract::After heating at 65 C, ribosomes isolated from Bacillus stearothermophilus were strikingly more heat-stable than comparable preparations from Escherichia coli when tested for ability to support polyuridylic acid-directed phenylalanine incorporation at 37 C. The stability of ribosomes was also determined by measurement...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.5.1521-1526.1967

    authors: Friedman SM,Axel R,Weinstein IB

    更新日期:1967-05-01 00:00:00

  • Biosynthesis of uridine diphosphate N-acetylmannosaminuronic acid in rff mutants of Salmonella tryphimurium.

    abstract::In Salmonella typhimurium, three groups of genes located in rfb, rfe, and rff clusters are known to be involved in the biosynthesis of the enterobacterial common antigen. We found that enzymatic synthesis of uridine diphosphate N-acetylmannosaminouric acid, the activated form of a constituent sugar of the common antig...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.1.227-233.1978

    authors: Lew HC,Nikaido H,Mäkelä PH

    更新日期:1978-10-01 00:00:00

  • Aspartate transcarbamylase synthesis ceases prior to inactivation of the enzyme in Bacillus subtilis.

    abstract::Aspartate transcarbamylase is synthesized during exponential growth of Bacillus subtilis and is inactivated when the cells enter the stationary phase. This work is a study of the regulation of aspartate transcarbamylase synthesis during growth and the stationary phase. Using specific immunoprecipitation of aspartate t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.3.943-951.1978

    authors: Maurizi MR,Switzer RL

    更新日期:1978-09-01 00:00:00

  • Dark incubation causes reinitiation of cell cycle events in Anacystis nidulans.

    abstract::Synchronized cultures of Anacystis nidulans (Synechococcus PCC 6301), an obligate phototroph, are obtained by incubating exponential cultures in the dark for 12 to 16 h. A temporal and sequential order of macromolecular synthesis is observed within the cell division cycle of a synchronously dividing culture in the lig...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.3.1315-1321.1983

    authors: Asato Y

    更新日期:1983-03-01 00:00:00

  • Alterations in the cytoplasmic membrane proteins of various chlorate-resistant mutants of Escherichia coli.

    abstract::Chlorate-resistant mutants corresponding to each known genetic locus (chlA, chlB, chlC, chlD, chlE) were isolated from Escherichia coli K-12. All these mutants showed decreased amounts of membrane-bound nitrate reductase, cytochrome b, and formic dehydrogenase, but all had normal succinic dehydrogenase activity. Prote...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.1.564-570.1971

    authors: MacGregor CH,Schnaitman CA

    更新日期:1971-10-01 00:00:00

  • Aquaporin Z of Escherichia coli: reassessment of its regulation and physiological role.

    abstract::Transcription of an aqpZ-lac fusion in a single copy on the Escherichia coli chromosome increased as cells entered the stationary growth phase. This was true in a variety of media, and increased transcription in enriched medium required the RpoS sigma factor. Expression of the aqpZ-lac fusion was not affected by up- o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.15.4304-4307.2002

    authors: Soupene E,King N,Lee H,Kustu S

    更新日期:2002-08-01 00:00:00

  • Regulation of Staphylococcus xylosus xylose utilization genes at the molecular level.

    abstract::We have investigated the regulation of the operon encoding xylose utilization in Staphylococcus xylosus C2a and Staphylococcus carnosus TM300. For in vivo studies, transcriptional fusions of the xylAB regulatory region to the lipase gene from Staphylococcus hyicus were constructed. Repression of lipase activity depend...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.9.3042-3048.1992

    authors: Sizemore C,Wieland B,Götz F,Hillen W

    更新日期:1992-05-01 00:00:00

  • Crystal structure of the PdxY Protein from Escherichia coli.

    abstract::The crystal structure of Escherichia coli PdxY, the protein product of the pdxY gene, has been determined to a 2.2-A resolution. PdxY is a member of the ribokinase superfamily of enzymes and has sequence homology with pyridoxal kinases that phosphorylate pyridoxal at the C-5' hydroxyl. The protein is a homodimer with ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.23.8074-8082.2004

    authors: Safo MK,Musayev FN,Hunt S,di Salvo ML,Scarsdale N,Schirch V

    更新日期:2004-12-01 00:00:00

  • Regulation of excision genes of the Bacteroides conjugative transposon CTnDOT.

    abstract::The first step in the transfer of the Bacteroides conjugative transposon CTnDOT is excision of the integrated element from the chromosome to form a circular transfer intermediate. Excision occurs only after the bacteria are exposed to tetracycline. Previously, four excision genes were identified. One was the integrase...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.16.5732-5741.2005

    authors: Moon K,Shoemaker NB,Gardner JF,Salyers AA

    更新日期:2005-08-01 00:00:00

  • Media dependence of commitment in Bacillus subtilis.

    abstract::At some time during sporulation development, cells of Bacillus subtilis develop a commitment to continue sporulation even after addition of or dilution into a fresh nutrient. The extent of commitment was measured by the titer of spores produced at the time at which the original culture sporulated maximally. Since newl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.2.901-907.1977

    authors: Cooney PH,Whiteman PF,Freese E

    更新日期:1977-02-01 00:00:00

  • Tripartite Regulation of the glpFKD Operon Involved in Glycerol Catabolism by GylR, Crp, and SigF in Mycobacterium smegmatis.

    abstract::The glpD (MSMEG_6761) gene encoding glycerol-3-phosphate dehydrogenase was shown to be crucial for M. smegmatis to utilize glycerol as the sole carbon source. The glpD gene likely forms the glpFKD operon together with glpF and glpK, encoding a glycerol facilitator and glycerol kinase, respectively. The gylR (MSMEG_675...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00511-19

    authors: Bong HJ,Ko EM,Song SY,Ko IJ,Oh JI

    更新日期:2019-11-20 00:00:00

  • Recognition of overlapping nucleotides by AraC and the sigma subunit of RNA polymerase.

    abstract::The Escherichia coli promoter p(BAD), under the control of the AraC protein, drives the expression of mRNA encoding the AraB, AraA, and AraD gene products of the arabinose operon. The binding site of AraC at p(BAD) overlaps the RNA polymerase -35 recognition region by 4 bases, leaving 2 bases of the region not contact...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.18.5076-5081.2000

    authors: Dhiman A,Schleif R

    更新日期:2000-09-01 00:00:00

  • Reconstitution of an active lactose carrier in vivo by simultaneous synthesis of two complementary protein fragments.

    abstract::Escherichia coli lactose permease mediates the proton-driven translocation of galactosides across the cytoplasmic membrane. To define regions important for membrane insertion as well as for biological function, we constructed plasmids encoding different portions of the lactose carrier. Among several lacY deletions, tw...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.9.5374-5381.1990

    authors: Wrubel W,Stochaj U,Sonnewald U,Theres C,Ehring R

    更新日期:1990-09-01 00:00:00

  • Regulation of arginase activity by intermediates of the arginine biosynthetic pathway in Neurospora crassa.

    abstract::It has been found that, in Neurospora crassa, arginine synthesized from exogenous citrulline was not as effectively hydrolyzed as exogenous arginine. This was explained by the observed inhibition of arginase in vitro and in vivo by citrulline. The high arginine pool formed from exogenous citrulline feedback inhibits t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.3.870-877.1972

    authors: Mora J,Salceda R,Sanchez S

    更新日期:1972-06-01 00:00:00

  • Physiological analysis of the stringent response elicited in an extreme thermophilic bacterium, Thermus thermophilus.

    abstract::Guanosine tetraphosphate (ppGpp) is a key mediator of stringent control, an adaptive response of bacteria to amino acid starvation, and has thus been termed a bacterial alarmone. Previous X-ray crystallographic analysis has provided a structural basis for the transcriptional regulation of RNA polymerase activity by pp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00574-06

    authors: Kasai K,Nishizawa T,Takahashi K,Hosaka T,Aoki H,Ochi K

    更新日期:2006-10-01 00:00:00

  • SULFATE REQUIREMENT FOR IRON OXIDATION BY THIOBACILLUS FERROOXIDANS.

    abstract::Lazaroff, Norman (British Columbia Research Council, Vancouver, B.C., Canada). Sulfate requirement for iron oxidation by Thiobacillus ferrooxidans. J. Bacteriol. 85:78-83. 1963.-The growth of Thiobacillus ferrooxidans is initially inhibited in media containing ferrous chloride in place of ferrous sulfate. This inhibit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.1.78-83.1963

    authors: Lazaroff N

    更新日期:1963-01-01 00:00:00

  • Swarming differentiation and swimming motility in Bacillus subtilis are controlled by swrA, a newly identified dicistronic operon.

    abstract::The number and disposition of flagella harbored by eubacteria are regulated by a specific trait successfully maintained over generations. The genes governing the number of flagella in Bacillus subtilis have never been identified, although the ifm locus has long been recognized to influence the motility phenotype of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.15.5356-5366.2005

    authors: Calvio C,Celandroni F,Ghelardi E,Amati G,Salvetti S,Ceciliani F,Galizzi A,Senesi S

    更新日期:2005-08-01 00:00:00

  • Genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5T.

    abstract::Oligotropha carboxidovorans OM5(T) (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium with the capability to utilize carbon monoxide, carbon dioxide, and hydrogen. It is also capable of heterotrophic growth under appropriate environmental conditions. Here we report the annotated genome sequence of the circula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00614-08

    authors: Paul D,Bridges S,Burgess SC,Dandass Y,Lawrence ML

    更新日期:2008-08-01 00:00:00

  • Microarray identification of Clostridium difficile core components and divergent regions associated with host origin.

    abstract::Clostridium difficile is a gram-positive, spore-forming enteric anaerobe which can infect humans and a wide variety of animal species. Recently, the incidence and severity of human C. difficile infection has markedly increased. In this study, we evaluated the genomic content of 73 C. difficile strains isolated from hu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00222-09

    authors: Janvilisri T,Scaria J,Thompson AD,Nicholson A,Limbago BM,Arroyo LG,Songer JG,Gröhn YT,Chang YF

    更新日期:2009-06-01 00:00:00

  • Lytic effects of staphylococcal alpha-toxin and delta-hemolysin.

    abstract::Several preparations of staphylococcal alpha-toxin and delta-lysin were studied in order to compare hemolytic activity with capacity to lyse bacterial protoplasts. delta-Lysin in relatively low concentration lysed protoplasts of Sarcina lutea, protoplasts of Streptococcus faecalis, and spheroplasts of Escherichia coli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.2.487-491.1968

    authors: Bernheimer AW,Avigad LS,Grushoff P

    更新日期:1968-08-01 00:00:00

  • Mutations affecting the citrate-dependent iron uptake system in Escherichia coli.

    abstract::Isolation of six strains of Escherichia coli K-12 carrying mutations affecting the citrate-dependent iron uptake system is described. Genetic analysis of these mutants showed that the mutation affecting the citrate system are cluster together at about min 6 on the E. coli chromosome. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.3.1524-1526.1978

    authors: Woodrow GC,Langman L,Young IG,Gibson F

    更新日期:1978-03-01 00:00:00