Substrate specificity of naphthalene dioxygenase: effect of specific amino acids at the active site of the enzyme.

abstract：:The outer membrane of the thermophilic bacterium Thermus thermophilus was isolated using sucrose step gradient centrifugation. Its detergent extracts contained an ion-permeable channel with an extremely high single-channel conductance of 20 nS in 1 M KCl. The channel protein was purified by preparative sodium dodecyl ...

journal_title：Journal of bacteriology

authors： Maier E,Polleichtner G,Boeck B,Schinzel R,Benz R

更新日期：2001-01-01 00:00:00

abstract：:Alicyclobacillus acidocaldarius strain Tc-4-1 was initially isolated from a hot spring in Tengchong, China. This organism is both thermophilic and acidophilic. It can produce heat- and acid-stable enzymes, such as amylase and esterase, which may be important in industry. Here we report the whole genome sequence of the...

journal_title：Journal of bacteriology

authors： Chen Y,He Y,Zhang B,Yang J,Li W,Dong Z,Hu S

更新日期：2011-10-01 00:00:00

abstract：:Rhodococcus equi is a facultative intracellular opportunistic pathogen of immunocompromised people and a major cause of pneumonia in young horses. An effective live attenuated vaccine would be extremely useful in the prevention of R. equi disease in horses. Toward that end, we have developed an efficient transposon mu...

journal_title：Journal of bacteriology

authors： Ashour J,Hondalus MK

更新日期：2003-04-01 00:00:00

abstract：:Lactobacillus rhamnosus R0011 is a commercially available probiotic that is widely used in human dietary supplements and pharmaceutical products. We prepared a draft genome sequence consisting of 10 contigs totaling 2,900,620 bases and a G+C content of 46.7% for this strain. ...

journal_title：Journal of bacteriology

authors： Tompkins TA,Barreau G,de Carvalho VG

更新日期：2012-02-01 00:00:00

abstract：:Grown in liquid culture in the presence of a variety of structurally unrelated drugs, mycelia of wild-type Neurospora assume a colonial or semicolonial growth habit similar to that of known morphological mutants. Drugs that produce these morphological changes include atropine, theophylline, histamine, and several of t...

journal_title：Journal of bacteriology

authors： Scott WA,Solomon B

更新日期：1975-05-01 00:00:00

abstract：:Pseudomonas aeruginosa PAO1 catabolized the aromatic amines tyramine and octopamine through 4-hydroxyphenylacetic acid and 3,4-dihydroxyphenylacetic acid (HPA). meta ring cleavage was mediated by 3,4-dihydroxyphenylacetate 2,3-dioxygenase (HPADO), producing 2-hydroxy-5-carboxymethylmuconic semialdehyde (MSA). An NAD-d...

journal_title：Journal of bacteriology

authors： Cuskey SM,Olsen RH

更新日期：1988-01-01 00:00:00

abstract：:Escherichia coli acetyl coenzyme A carboxylase (ACC), the first enzyme of the fatty acid biosynthetic pathway, is inhibited by acylated derivatives of acyl carrier protein (ACP). ACP lacking an acyl moiety does not inhibit ACC. Acylated derivatives of ACP having chain lengths of 6 to 20 carbon atoms were similarly inh...

journal_title：Journal of bacteriology

authors： Davis MS,Cronan JE Jr

更新日期：2001-02-01 00:00:00

abstract：:Xylella fastidiosa possesses both type I and type IV pili at the same cell pole. By use of a microfluidic device, the speed of twitching movement by wild-type cells on a glass surface against the flow direction of media was measured as 0.86 (standard error [SE], 0.04) microm min(-1). A type I pilus mutant (fimA) moved...

journal_title：Journal of bacteriology

authors： De La Fuente L,Burr TJ,Hoch HC

更新日期：2007-10-01 00:00:00

abstract：:Group B streptococcus (GBS) pili may enhance colonization and infection by mediating bacterial adhesion to host cells, invasion across endothelial and epithelial barriers, and resistance to bacterial ingestion and killing by host phagocytes. However, it remains unclear how pilus expression is regulated and how modulat...

journal_title：Journal of bacteriology

authors： Jiang S,Park SE,Yadav P,Paoletti LC,Wessels MR

更新日期：2012-05-01 00:00:00

abstract：:A new class II (Tn3-like) transposable element, designated Tn5401, was recovered from a sporulation-deficient variant of Bacillus thuringiensis subsp. morrisoni EG2158 following its insertion into a recombinant plasmid. Sequence analysis of the insert revealed a 4,837-bp transposon with two large open reading frames, ...

journal_title：Journal of bacteriology

authors： Baum JA

更新日期：1994-05-01 00:00:00

abstract：:Induction of nod genes in Rhizobium and Bradyrhizobium species is dependent on the presence of plant-produced flavonoids, the NodD protein, and the cis-acting nod box promoter sequence. Although the nodD (nodD1) gene in Rhizobium species is constitutively expressed, nodD1 expression in Bradyrhizobium japonicum is indu...

journal_title：Journal of bacteriology

authors： Wang SP,Stacey G

更新日期：1991-06-01 00:00:00

abstract：:The uptake of iron into Pseudomonas aeruginosa is mediated by two major siderophores produced by the bacterium, pyoverdine and pyochelin. The bacterium is also able of utilize several heterologous siderophores of bacterial or fungal origin. In this work, we have investigated the iron uptake in P. aeruginosa PAO1 by th...

journal_title：Journal of bacteriology

authors： Hannauer M,Barda Y,Mislin GL,Shanzer A,Schalk IJ

更新日期：2010-03-01 00:00:00

abstract：:Isolated membranes of Bacillus stearothermophilus 2184D can be disrupted by treatment with sodium dodecyl sulfate (SDS). This disruption is attended by a decreased turbidity of membrane suspensions and a differential loss of activities of the electron transport system. Reduced methyl viologen (MVH)-nitrate reductase a...

journal_title：Journal of bacteriology

authors： Kiszkiss DF,Downey RJ

更新日期：1972-02-01 00:00:00

abstract：:Lysozyme is an important component of the innate immune system. It functions by hydrolyzing the peptidoglycan (PG) layer of bacteria. The human pathogen Listeria monocytogenes is intrinsically lysozyme resistant. The peptidoglycan N-deacetylase PgdA and O-acetyltransferase OatA are two known factors contributing to it...

journal_title：Journal of bacteriology

authors： Rismondo J,Schulz LM,Yacoub M,Wadhawan A,Hoppert M,Dionne MS,Gründling A

更新日期：2021-01-25 00:00:00

abstract：:Klebsiella pneumoniae porin genes were analyzed to detect mutations accounting for the porin deficiency observed in many beta-lactam-resistant strains. PCR and Southern blot analysis revealed the existence of a third porin gene in addition to the OmpK36 and OmpK35 porin genes previously described. This new porin gene ...

journal_title：Journal of bacteriology

authors： Doménech-Sánchez A,Hernández-Allés S,Martínez-Martínez L,Benedí VJ,Albertí S

更新日期：1999-05-01 00:00:00

abstract：:The alpha-aminoadipate pathway for the biosynthesis of lysine is present only in fungi and euglena. Until now, this unique metabolic pathway has never been investigated in the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. Five of the eight enzymes (homocitrate syn...

journal_title：Journal of bacteriology

authors： Garrad RC,Bhattacharjee JK

更新日期：1992-11-01 00:00:00

abstract：:The peptidoglycan cell wall determines the shape and structural integrity of a bacterial cell. Class B penicillin-binding proteins (PBPs) carry a transpeptidase activity that cross-links peptidoglycan strands via their peptide side chains, and some of these proteins are directly involved in cell shape determination. N...

journal_title：Journal of bacteriology

authors： Wei Y,Havasy T,McPherson DC,Popham DL

更新日期：2003-08-01 00:00:00

abstract：:Mutant strains of Aspergillus niger with reduced citrate control of carbohydrate catabolism (cic mutants) grow faster than the parent strain on media containing 5% (wt/vol) citrate. The mutants tolerated a higher intracellular citrate concentration than the parent strain. One mutant (cic-7/3) contained phosphofructoki...

journal_title：Journal of bacteriology

authors： Schreferl G,Kubicek CP,Röhr M

更新日期：1986-03-01 00:00:00

abstract：:Thorne, Curtis B. (Fort Detrick, Frederick, Md.). Transduction in Bacillus subtilis. J. Bacteriol. 83:106-111. 1962.-A bacteriophage, SP-10, isolated from soil carries out general transduction in Bacillus subtilis. Phage propagated on a streptomycin-resistant mutant of the wild-type strain W-23 was capable of transduc...

journal_title：Journal of bacteriology

authors： THORNE CB

更新日期：1962-01-01 00:00:00

abstract：:A genetic element integrating site specifically into a prokaryotic gene usually carries a copy of the 3' portion of that gene that restores the active gene even as the original is disrupted. A cryptic element in Mesorhizobium loti instead carries a copy of the 5' end of the tRNA gene into which it integrated. This has...

journal_title：Journal of bacteriology

authors： Zhao S,Williams KP

更新日期：2002-02-01 00:00:00

abstract：:We analyzed expression of a putative oligopeptide permease (Opp) of Borrelia burgdorferi. Unlike the opp operons of other bacteria for which there is a single substrate binding protein, B. burgdorferi codes for three substrate binding proteins (OppA-I to -III) in its opp operon and an additional two homologs on plasmi...

journal_title：Journal of bacteriology

authors： Wang XG,Lin B,Kidder JM,Telford S,Hu LT

更新日期：2002-11-01 00:00:00

abstract：:TolB is a periplasmic protein of the cell envelope Tol complex. It is partially membrane associated through an interaction with the outer membrane lipoprotein PAL (peptidoglycan-associated lipoprotein), which also belongs to the Tol system. The interaction of TolB with outer membrane porins of Escherichia coli was inv...

journal_title：Journal of bacteriology

authors： Rigal A,Bouveret E,Lloubes R,Lazdunski C,Benedetti H

更新日期：1997-12-01 00:00:00

abstract：:Vibrio vulnificus is a human pathogen that produces lethal septicemia in susceptible persons, and the primary virulence factor for this organism is capsular polysaccharide (CPS). The role of the capsule in V. vulnificus biofilms was examined under a variety of conditions, by using either defined CPS mutants or spontan...

journal_title：Journal of bacteriology

authors： Joseph LA,Wright AC

更新日期：2004-02-01 00:00:00

abstract：:Six analogues of amino acids killed corresponding auxotrophs of Escherichia coli. With all but one analogue, protein synthesis was required for lethality. ...

journal_title：Journal of bacteriology

authors： Rabinovitz M,Finkleman A,Reagan RL,Breitman TR

更新日期：1969-07-01 00:00:00

abstract：:soxR governs a superoxide response regulon that contains the genes for endonuclease IV, Mn2(+)-superoxide dismutase, and glucose 6-phosphate dehydrogenase. The soxR gene encodes a 17-kDa protein; some mutations of this gene cause constitutive overexpression of the regulon. Induction by paraquat (methyl viologen) requi...

journal_title：Journal of bacteriology

authors： Wu J,Weiss B

更新日期：1991-05-01 00:00:00

abstract：:Cellular vanadium metabolism was studied in Saccharomyces cerevisiae by isolating and characterizing vanadate [VO4(3-), V(V)]-resistant mutants. Vanadate growth inhibition was reversed by the removal of the vanadate from the medium, and vanadate resistance was found to be a recessive trait. Vanadate-resistant mutants ...

journal_title：Journal of bacteriology

authors： Willsky GR,Leung JO,Offermann PV Jr,Plotnick EK,Dosch SF

更新日期：1985-11-01 00:00:00

abstract：:In response to DNA damage or the inhibition of normal DNA replication in Escherichia coli, a set of some 20 unlinked operons is induced through the RecA-mediated cleavage of the LexA repressor. We examined the effect of this SOS response on the transposition of Tn5 and determined that the frequency of transposition is...

journal_title：Journal of bacteriology

authors： Weinreich MD,Makris JC,Reznikoff WS

更新日期：1991-11-01 00:00:00

abstract：:Lipoprotein anchoring in bacteria is mediated by the prolipoprotein diacylglyceryl transferase (Lgt), which catalyzes the transfer of a diacylglyceryl moiety to the prospective N-terminal cysteine of the mature lipoprotein. Deletion of the lgt gene in the gram-positive pathogen Listeria monocytogenes (i) impairs intra...

journal_title：Journal of bacteriology

authors： Baumgärtner M,Kärst U,Gerstel B,Loessner M,Wehland J,Jänsch L

更新日期：2007-01-01 00:00:00

abstract：:The transcriptional control of the kdpFABC (K+ transport) operon of Salmonella typhimurium was characterized with a lacZ fusion. The kdpFABC operon of this organism was induced by K+ limitation and high osmolality, and osmotic induction was antagonized by a high concentration of K+. In the trkA (sapG) kdp+ mutant back...

journal_title：Journal of bacteriology

authors： Frymier JS,Reed TD,Fletcher SA,Csonka LN

更新日期：1997-05-01 00:00:00

abstract：:Penicillin-binding proteins of 180, 89, 80, 68, 61, 41, and 38 kilodaltons were identified in Treponema pallidum (Nichols) by their covalent binding of [35S]benzylpenicillin. Penicillin-binding proteins are localized in the plasma membranes of many bacterial species and may serve as useful markers for determining plas...

journal_title：Journal of bacteriology

authors： Cunningham TM,Miller JN,Lovett MA

更新日期：1987-11-01 00:00:00