Plasmid and transposon transfer to Thiobacillus ferrooxidans.

abstract：:Respiration-deficient mutants (Rho-, petite) of Saccharomyces carlsbergensis were obtained by treatment with trypaflavin (euflavine). Dried cells of these mutants phosphorylated mononucleotides to their triphosphates and further formed not only cytidine 5'-diphosphate-choline, but also sugar nucleotides, such as uridi...

journal_title：Journal of bacteriology

authors： Kimura A,Hirose K,Kariya Y,Nagai S

更新日期：1976-02-01 00:00:00

abstract：:CTXphi is a filamentous bacteriophage that encodes cholera toxin. CTXphi infection of its host bacterium, Vibrio cholerae, requires the toxin-coregulated pilus (TCP) and the products of the V. cholerae tolQRA genes. Here, we have explored the role of OrfU, a predicted CTXphi minor coat protein, in CTXphi infection. Pr...

journal_title：Journal of bacteriology

authors： Heilpern AJ,Waldor MK

更新日期：2003-02-01 00:00:00

abstract：:Two activites causing nitrite disappearance are found in extracts of Neurospora; one, inducible by nitrate or nitrite and present only in nitrite-utilizing strains, catalyze the stoichiometric reduction of nitrite to ammonia; the other, present in all strains under all conditions, causes the disappearance of nitrite t...

journal_title：Journal of bacteriology

authors： Chang HC,Mulkins GJ,Dyer JC,Sorger GJ

更新日期：1975-08-01 00:00:00

abstract：:The CpxA-CpxR two-component signal transduction pathway of Escherichia coli was studied in a mutant (pss-93) lacking phosphatidylethanolamine (PE). Several properties of this mutant are comparable to phenotypes of cpxA point mutants, indicating that this two-component pathway is activated in PE-deficient cells. In con...

journal_title：Journal of bacteriology

authors： Mileykovskaya E,Dowhan W

更新日期：1997-02-01 00:00:00

abstract：:A gene encoding only the C-terminal portion of the receptor-transducer protein Tar of Escherichia coli was constructed. The gene product was detected and localized in the cytoplasmic fraction of the cell by immunoblotting with anti-Tar antibodies. The C-terminal fragments from wild-type and mutant tar genes were chara...

journal_title：Journal of bacteriology

authors： Oosawa K,Mutoh N,Simon MI

更新日期：1988-06-01 00:00:00

abstract：:Cells of Haemophilus influenzae strain Rd competent for genetic transformation irreversibly bound approximately five molecular fragments of H. influenzae deoxyribonucleic acid (DNA) per cell; under identical conditions, DNA derived from Escherichia coli B was not taken up (<1 molecule per 50 cells). Similarly, DNA fro...

journal_title：Journal of bacteriology

authors： Scocca JJ,Poland RL,Zoon KC

更新日期：1974-05-01 00:00:00

abstract：:A simple three-step procedure is described which yields electrophoretically homogeneous preparations of ribitol-5-phosphate dehydrogenase and xylitol-5-phosphate dehydrogenase. The former enzyme is a 115,000-molecular-weight protein composed of two subunits of identical size and is specific for its substrate, ribitol....

journal_title：Journal of bacteriology

authors： Hausman SZ,London J

更新日期：1987-04-01 00:00:00

abstract：:The plasmid-encoded yop genes of pathogenic yersiniae are regulated by the environmental stimuli calcium and temperature. A novel protein, LcrQ, which exhibits a key function in the negative calcium-controlled pathway, was identified. DNA sequence analysis revealed that LcrQ has a molecular mass of 12,412 daltons and ...

journal_title：Journal of bacteriology

authors： Rimpiläinen M,Forsberg A,Wolf-Watz H

更新日期：1992-05-01 00:00:00

abstract：:The expression of R-factor-mediated resistance to tetracycline has been compared in Proteus mirabilis and Escherichia coli. Resistance to a range of concentrations of tetracycline was significantly lower in P. mirabilis than in E. coli in both induced and repressed states. Indirect evidence showed that conditions whic...

journal_title：Journal of bacteriology

authors： Franklin TJ,Rownd R

更新日期：1973-07-01 00:00:00

abstract：:Two regulatory proteins, Gal repressor and isorepressor, control the expression of the gal and mgl operons in Escherichia coli. The transcription start sites for galR and galS, the genes for the repressor and isorepressor, were determined by primer extension of in vivo transcripts. Study of the promoter-lacZ gene fusi...

journal_title：Journal of bacteriology

authors： Weickert MJ,Adhya S

更新日期：1993-01-01 00:00:00

abstract：:Streptococcus agalactiae (group B streptococcus [GBS]) is a major cause of infections in newborns, pregnant women, and immunocompromised patients. GBS strain CNCTC10/84 is a clinical isolate that has high virulence in animal models of infection and has been used extensively to study GBS pathogenesis. Two unusual featu...

journal_title：Journal of bacteriology

authors： Zhu L,Beres SB,Yerramilli P,Pruitt L,Cantu CC,Olsen RJ,Musser JM

更新日期：2020-11-04 00:00:00

abstract：:Most mutations in the yajC-secDF operon identified via genetic screens confer a cold-sensitive growth phenotype. Here we report that two of these mutations confer this cold-sensitive phenotype by inactivating the SecDF-YajC complex in protein translocation. ...

journal_title：Journal of bacteriology

authors： Nouwen N,Driessen AJ

更新日期：2005-10-01 00:00:00

abstract：:The first enzymes of the histidine (hut) and proline degradative pathways, histidase and proline oxidase, could not be induced in Bacillus subtilis cells growing in glucose minimal medium containing a mixture of 16 amino acids. Addition of the 16-amino-acid mixture to induced wild-type cells growing in citrate minimal...

journal_title：Journal of bacteriology

authors： Atkinson MR,Wray LV Jr,Fisher SH

更新日期：1990-09-01 00:00:00

abstract：:Nitroheterocyclic and nitroaromatic compounds constitute an enormous range of chemicals whose potent biological activity has significant human health and environmental implications. The biological activity of nitro-substituted compounds is derived from reductive metabolism of the nitro moiety, a process catalyzed by a...

journal_title：Journal of bacteriology

authors： Whiteway J,Koziarz P,Veall J,Sandhu N,Kumar P,Hoecher B,Lambert IB

更新日期：1998-11-01 00:00:00

abstract：:The developmentally regulated gene dofA, identified from pulse-labeling experiments by two-dimensional gel electrophoresis, and its homologue, dofB, were cloned and characterized in Myxococcus xanthus. Deletion of dofA and dofB did not affect the vegetative growth and development of M. xanthus. dofA was specifically e...

journal_title：Journal of bacteriology

authors： Horiuchi T,Akiyama T,Inouye S,Komano T

更新日期：2002-12-01 00:00:00

abstract：:Siderophore-mediated iron transport in Pseudomonas aeruginosa is dependent upon the cytoplasmic membrane-associated TonB1 energy coupling protein for activity. To assess the functional significance of the various regions of this molecule and to identify functionally important residues, the tonB1 gene was subjected to ...

journal_title：Journal of bacteriology

authors： Zhao Q,Poole K

更新日期：2002-03-01 00:00:00

abstract：:Liquid holding recovery (LHR) in ultraviolet-irradiated Bacteroides fragilis cells occurred under aerobic conditions but was inhibited by anaerobic conditions. The increase in survival after aerobic LHR resulted in an increase in the shoulder regions of the ultraviolet survival curves. Maximum LHR was obtained after h...

journal_title：Journal of bacteriology

authors： Jones DT,Woods DR

更新日期：1981-01-01 00:00:00

abstract：:The biodegradative threonine dehydratase (tdc) operon was mapped at 68 min on the Escherichia coli K-12 chromosome. The order of markers in the clockwise direction was dnaG uxaA tdc argG. A tdc deletion was isolated and mapped to this region of the chromosome. By using a tdcB-lacZ fusion the clockwise direction of tra...

journal_title：Journal of bacteriology

authors： Schweizer HP,Datta P

更新日期：1988-11-01 00:00:00

abstract：:Escherichia coli LD0181 is sensitive to 15 micrograms of 2',3'-dideoxythymidine per ml. A derivative that was resistant to 40 micrograms of the same chemical per ml at 30 degrees C and that had lost the ability to grow on enriched medium at 42 degrees C was isolated after nitroso-guanidine mutagenesis. This mutant, TD...

journal_title：Journal of bacteriology

authors： Daws TD,Fuchs JA

更新日期：1984-02-01 00:00:00

abstract：:We examined the predacious gram-negative bacterium Bdellovibrio bacteriovorous 109J and free-living strains 109J-A1 and 109J-KA1 derived therefrom for penicillin-binding proteins (PBPs). We compared their PBPs with those of the host bacterium, Escherichia coli, and with those of a facultatively predacious bdellovibrio...

journal_title：Journal of bacteriology

authors： Park JT,Mahadevan S

更新日期：1988-08-01 00:00:00

abstract：:Acclimation to high salt concentrations involves concerted changes in gene expression. For the majority of salt-regulated genes, the mechanism underlying the induction process is not known. The gene ggpS (sll1566), which encodes the glucosylglycerol-phosphate synthase responsible for the synthesis of the compatible so...

journal_title：Journal of bacteriology

authors： Klähn S,Höhne A,Simon E,Hagemann M

更新日期：2010-09-01 00:00:00

abstract：:The nucleotide sequence of the Clostridium thermocellum F1 xynC gene, which encodes the xylanase XynC, consists of 1,857 bp and encodes a protein of 619 amino acids with a molecular weight of 69,517. XynC contains a typical N-terminal signal peptide of 32 amino acid residues, followed by a 165-amino-acid sequence whic...

journal_title：Journal of bacteriology

authors： Hayashi H,Takagi KI,Fukumura M,Kimura T,Karita S,Sakka K,Ohmiya K

更新日期：1997-07-01 00:00:00

abstract：:A specialized transducing phage lambda carrying the structural gene for the OmpF protein, an outer membrane matrix protein, was isolated. The phage carries the 20.5--21-min region of the Escherichia coli K-12 chromosome and carries asnS, ompF, and aspC genes. ...

journal_title：Journal of bacteriology

authors： Mutoh N,Nagasawa T,Mizushima S

更新日期：1981-02-01 00:00:00

abstract：:Formate dehydrogenase has traditionally been assumed to play an essential role in energy generation during growth on C(1) compounds. However, this assumption has not yet been experimentally tested in methylotrophic bacteria. In this study, a whole-genome analysis approach was used to identify three different formate d...

journal_title：Journal of bacteriology

authors： Chistoserdova L,Laukel M,Portais JC,Vorholt JA,Lidstrom ME

更新日期：2004-01-01 00:00:00

abstract：:The bacterial phosphoenolpyruvate phosphotransferase system (PTS) is a highly conserved phosphotransfer cascade that participates in the transport and phosphorylation of selected carbohydrates and modulates many cellular functions in response to carbohydrate availability. It plays a role in the virulence of many bacte...

journal_title：Journal of bacteriology

authors： Houot L,Chang S,Pickering BS,Absalon C,Watnick PI

更新日期：2010-06-01 00:00:00

abstract：:A key reaction in the biosynthesis of menaquinone involves the conversion of the soluble bicyclic naphthalenoid compound 1, 4-dihydroxy-2-naphthoic acid (DHNA) to the membrane-bound demethylmenaquinone. The enzyme catalyzing this reaction, DHNA-octaprenyltransferase, attaches a 40-carbon side chain to DHNA. The menA g...

journal_title：Journal of bacteriology

authors： Suvarna K,Stevenson D,Meganathan R,Hudspeth ME

更新日期：1998-05-01 00:00:00

abstract：:We have identified three transposable gene-activating elements from Pseudomonas cepacia on the basis of their abilities to increase expression of the lac genes of the broad-host-range plasmid pGC91.14 (pRP1::Tn951). When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), this plasmid failed to con...

journal_title：Journal of bacteriology

authors： Wood MS,Lory C,Lessie TG

更新日期：1990-04-01 00:00:00

abstract：:Bishop, D. G. (University of Sydney, Sydney, Australia), and J. L. Still. Fatty acid metabolism in Serratia marcescens. J. Bacteriol. 82:370-375. 1961.-A study has been made of the oxidation of saturated fatty acids containing between two and 18 carbon atoms by whole cells of the bacterium, Serratia marcescens. This o...

journal_title：Journal of bacteriology

authors： Bishop DG,Still JL

更新日期：1961-09-01 00:00:00

abstract：:The salmonellae are a diverse group of bacteria within the family Enterobacteriaceae that includes two species, Salmonella enterica and Salmonella bongori. In order to characterize the phylogenetic relationships of the species and subspecies of Salmonella, we analyzed four housekeeping genes, gapA, phoP, mdh and recA,...

journal_title：Journal of bacteriology

authors： McQuiston JR,Herrera-Leon S,Wertheim BC,Doyle J,Fields PI,Tauxe RV,Logsdon JM Jr

更新日期：2008-11-01 00:00:00

abstract：:The phoP-phoQ operon of Salmonella typhimurium is a member of the family of two-component regulatory systems and controls expression of the phoN gene that codes for nonspecific acid phosphatase and the genes involved in the pathogenicity of the bacterium. The phoP-phoQ operon of Escherichia coli was cloned on a plasmi...

journal_title：Journal of bacteriology

authors： Kasahara M,Nakata A,Shinagawa H

更新日期：1992-01-01 00:00:00