PAS domain of the Aer redox sensor requires C-terminal residues for native-fold formation and flavin adenine dinucleotide binding.

Abstract:

:The Aer protein in Escherichia coli is a membrane-bound, FAD-containing aerotaxis and energy sensor that putatively monitors the redox state of the electron transport system. Binding of FAD to Aer requires the N-terminal PAS domain and residues in the F1 region and C-terminal HAMP domain. The PAS domains of other PAS proteins are soluble in water. To investigate properties of the PAS domain, we subcloned segments of the aer gene from E. coli that encode the PAS domain with and without His6 tags and expressed the PAS peptides in E. coli. The 20-kDa His6-Aer2-166 PAS-F1 fragment was purified as an 800-kDa complex by gel filtration chromatography, and the associating protein was identified by N-terminal sequencing as the chaperone protein GroEL. None of the N-terminal fragments of Aer found in the soluble fraction was released from GroEL, suggesting that these peptides do not fold correctly in an aqueous environment and require a motif external to the PAS domain for proper folding. Consistent with this model, peptide fragments that included the membrane binding region and part (Aer2-231) or all (Aer2-285) of the HAMP domain inserted into the membrane, indicating that they were released by GroEL. Aer2-285, but not Aer2-231, bound FAD, confirming the requirement for the HAMP domain in stabilizing FAD binding. The results raise an interesting possibility that residues outside the PAS domain that are required for FAD binding are essential for formation of the PAS native fold.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Herrmann S,Ma Q,Johnson MS,Repik AV,Taylor BL

doi

10.1128/JB.186.20.6782-6791.2004

keywords:

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

6782-91

issue

20

eissn

0021-9193

issn

1098-5530

pii

186/20/6782

journal_volume

186

pub_type

杂志文章
  • Genome sequence of Lactococcus garvieae 8831, isolated from rainbow trout lactococcosis outbreaks in Spain.

    abstract::Lactococcus garvieae is the etiological agent of lactococcosis, one of the most important disease threats to the sustainability of the rainbow trout farming industry. Here, we present the draft genome sequence of Lactococcus garvieae strain 8831, isolated from diseased rainbow trout, which is composed of 2,087,276 bp ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05326-11

    authors: Aguado-Urda M,López-Campos GH,Gibello A,Cutuli MT,López-Alonso V,Fernández-Garayzábal JF,Blanco MM

    更新日期:2011-08-01 00:00:00

  • Regulation of the arginine dihydrolase pathway in Clostridium sporogenes.

    abstract::Arginine deiminase activity was induced during the vegetative growth of Clostridium sporogenes. The enzyme was sensitive to catabolite repression. The other enzymes of the arginine dihydrolase pathway, namely, ornithine carbamoyl-transferase and carbamate kinase, did not show such variation. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.131.2.693-695.1977

    authors: Venugopal V,Nadkarni GB

    更新日期:1977-08-01 00:00:00

  • Analysis of DNA inversions in the shufflon of plasmid R64.

    abstract::The shufflon, a multiple DNA inversion system in the plasmid R64, consists of four DNA segments flanked and separated by seven 19-bp repeat sequences. Site-specific recombinations mediated by the rci product occur between each inverted repeat sequence, resulting in inversions of the four segments independently or in g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.6.1867-1871.1997

    authors: Gyohda A,Funayama N,Komano T

    更新日期:1997-03-01 00:00:00

  • Amplification and deletion of the amyE+-tmrB+ gene region in a Bacillus subtilis recombinant-phage genome by the tmrA7 mutation.

    abstract::A 22.4-kilobase DNA fragment containing the tmrA7-amyR2-amyE+-tmrB+-aroI+ region of the Bacillus subtilis N7 chromosomal DNA was cloned into a recombinant B. subtilis bacteriophage, p11-AA248. The amyE+-tmrB+ gene region, approximately 12.6 kilobases, in the phage genome was amplified in a tunicamycin-resistant (Tmr) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.165.2.549-556.1986

    authors: Furusato T,Takano J,Yamane K,Hashiguchi K,Tanimoto A,Mori M,Yoda K,Yamasaki M,Tamura G

    更新日期:1986-02-01 00:00:00

  • Induction of the SOS response in Escherichia coli inhibits Tn5 and IS50 transposition.

    abstract::In response to DNA damage or the inhibition of normal DNA replication in Escherichia coli, a set of some 20 unlinked operons is induced through the RecA-mediated cleavage of the LexA repressor. We examined the effect of this SOS response on the transposition of Tn5 and determined that the frequency of transposition is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.21.6910-6918.1991

    authors: Weinreich MD,Makris JC,Reznikoff WS

    更新日期:1991-11-01 00:00:00

  • SufS is a NifS-like protein, and SufD is necessary for stability of the [2Fe-2S] FhuF protein in Escherichia coli.

    abstract::Escherichia coli fhuF mutants, a sufS::MudI mutant, and a sufD::MudI mutant were found to have the same phenotype: the inability to use ferrioxamine B as an iron source in a plate assay. In addition, the sufS and sufD genes were shown to be regulated by the iron-dependent Fur repressor. Sequence analysis revealed that...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.10.3307-3309.1999

    authors: Patzer SI,Hantke K

    更新日期:1999-05-01 00:00:00

  • Antibiotic Korormicin A Kills Bacteria by Producing Reactive Oxygen Species.

    abstract::Korormicin is an antibiotic produced by some pseudoalteromonads which selectively kills Gram-negative bacteria that express the Na+-pumping NADH:quinone oxidoreductase (Na+-NQR.) We show that although korormicin is an inhibitor of Na+-NQR, the antibiotic action is not a direct result of inhibiting enzyme activity. Ins...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00718-18

    authors: Maynard A,Butler NL,Ito T,da Silva AJ,Murai M,Chen T,Koffas MAG,Miyoshi H,Barquera B

    更新日期:2019-05-08 00:00:00

  • Staphylococcus aureus cap5P encodes a UDP-N-acetylglucosamine 2-epimerase with functional redundancy.

    abstract::The serotype 5 capsule gene cluster of Staphylococcus aureus comprises 16 genes (cap5A through cap5P), but little is known about how the putative gene products function in capsule biosynthesis. We propose that the N-acetylmannosaminuronic acid (ManNAcA) component of the S. aureus serotype 5 capsular polysaccharide (CP...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.16.4818-4824.1999

    authors: Kiser KB,Bhasin N,Deng L,Lee JC

    更新日期:1999-08-01 00:00:00

  • Aspartate aminotransferase activity is required for aspartate catabolism and symbiotic nitrogen fixation in Rhizobium meliloti.

    abstract::A mutant of Rhizobium meliloti, 4R3, which is unable to grow on aspartate has been isolated. The defect is specific to aspartate utilization, since 4R3 is not an auxotroph and grows as well as its parent strain on other carbon and nitrogen sources. The defect was correlated with an inability to fix nitrogen within nod...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.9.2879-2887.1991

    authors: Rastogi VK,Watson RJ

    更新日期:1991-05-01 00:00:00

  • Utilization of nitrate by pathogenic and saprophytic mycobacteria.

    abstract::Hedgecock, L. W. (Veterans Administration Hospital, Kansas City, Mo.) and R. L. Costello. Utilization of nitrate by pathogenic and saprophytic mycobacteria. J. Bacteriol. 84:195-205. 1962-The ability of mycobacteria to utilize nitrate as a sole source of nitrogen was examined. Nitrate-nitrogen was readily utilized by ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.2.195-205.1962

    authors: HEDGECOCK LW,COSTELLO RL

    更新日期:1962-08-01 00:00:00

  • Exfoliatin-producing strains define a fourth agr specificity group in Staphylococcus aureus.

    abstract::The staphylococcal virulon is activated by the density-sensing agr system, which is autoinduced by a short peptide (autoinducing peptide [AIP]) processed from a propeptide encoded by agrD. A central segment of the agr locus, consisting of the C-terminal two-thirds of AgrB (the putative processing enzyme), AgrD, and th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.22.6517-6522.2000

    authors: Jarraud S,Lyon GJ,Figueiredo AM,Lina G,Vandenesch F,Etienne J,Muir TW,Novick RP

    更新日期:2000-11-01 00:00:00

  • Phototaxis and impaired motility in adenylyl cyclase and cyclase receptor protein mutants of Synechocystis sp. strain PCC 6803.

    abstract::We have carefully characterized and reexamined the motility and phototactic responses of Synechocystis sp. adenylyl cyclase (Cya1) and catabolite activator protein (SYCRP1) mutants to different light regimens, glucose, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, and cyclic AMP. We find that contrary to earlier reports, c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00573-06

    authors: Bhaya D,Nakasugi K,Fazeli F,Burriesci MS

    更新日期:2006-10-01 00:00:00

  • Site-specific recombination system encoded by toluene catabolic transposon Tn4651.

    abstract::The 56-kb class II toluene catabolic transposon Tn4651 from Pseudomonas putida plasmid pWW0 is unique in that (i) its efficient resolution requires, in addition to the 0.2-kb resolution (res) site, the two gene products TnpS and TnpT and (ii) the 2.4-kb tnpT-res-tnpS region is 48 kb apart from the tnpA gene (M. Tsuda,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.17.4757-4766.2002

    authors: Genka H,Nagata Y,Tsuda M

    更新日期:2002-09-01 00:00:00

  • Protein difference between competent and noncompetent cultures of a group H Streptococcus.

    abstract::Acidified phenol extracts prepared from competent cultures of a group H Streptococcus strain Wicky made competent with competence factor derived from cultures of another group H Streptococcus, strain Challis, showed a difference in polyacrylamide-gel protein patterns when compared to extracts prepared from noncompeten...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.360-362.1970

    authors: Ranhand JM,Theodore TS,Cole RM

    更新日期:1970-10-01 00:00:00

  • Regulatory aspects of L-glutamate transport in Aspergillus nidulans.

    abstract::Wild-type cells of Aspergillus nidulans synthesize a transport system which appears to be specific for l-glutamate and l-aspartate. The system is energy dependent and concentrates l-glutamate at least 60-fold. In cells grown in the presence of 1% sucrose, l-glutamate uptake activity is regulated by ammonium control, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.2.534-542.1974

    authors: Pateman JA,Kinghorn JR,Dunn E

    更新日期:1974-08-01 00:00:00

  • Mobile cytochrome c2 and membrane-anchored cytochrome cy are both efficient electron donors to the cbb3- and aa3-type cytochrome c oxidases during respiratory growth of Rhodobacter sphaeroides.

    abstract::We have recently established that the facultative phototrophic bacterium Rhodobacter sphaeroides, like the closely related Rhodobacter capsulatus species, contains both the previously characterized mobile electron carrier cytochrome c2 (cyt c2) and the more recently discovered membrane-anchored cyt cy. However, R. sph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.6.2013-2024.2001

    authors: Daldal F,Mandaci S,Winterstein C,Myllykallio H,Duyck K,Zannoni D

    更新日期:2001-03-01 00:00:00

  • Are thiosulfate and trithionate intermediates in dissimilatory sulfate reduction?

    abstract::The fate of 35-S during anaerobic metabolism of [35-S]sulfate, [35-S]thiosulfate, and [35-S]sulfate plus unlabeled thiosulfate by washed cell suspensions of Desulfovibrio spp, and of [35-S]thiosulfate by growing D. desulfuricans was examined. The results appear to be inconsistent with the hypothesis that thiosulfate i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.1.36-40.1975

    authors: Chambers LA,Trudinger PA

    更新日期:1975-07-01 00:00:00

  • Fatty acid composition of L-forms of Streptococcus faecalis cultured at different osmolalities.

    abstract::The fatty acid composition of the membranes of three different penicillin-produced L-forms of Streptococcus faecalis was determined: (i) a stable (nonreverting) L-form (T(53)) cultured in brain heart infusion (BHI) with 0.5 M sucrose; (ii) a stable L-form (T(531)) cultured in BHI without sucrose; and (iii) an unstable...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.1.73-75.1973

    authors: Montgomerie JZ,Kalmanson GM,Guze LB

    更新日期:1973-07-01 00:00:00

  • Spontaneous missense mutations in the rplX gene for ribosomal protein L24 from Escherichia coli.

    abstract::Temperature-resistant pseudorevertants of the temperature-sensitive Escherichia coli mutant KNS19, harboring a mutation in rplX, the gene for ribosomal protein L24, were isolated, cloned, and sequenced. The codon GAC for the amino acid Asp in the temperature-sensitive mutant corresponding to position 84 in the protein...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.10.4854-4856.1987

    authors: Nishi K,Müller M,Schnier J

    更新日期:1987-10-01 00:00:00

  • Physiological effects of Crl in Salmonella are modulated by sigmaS level and promoter specificity.

    abstract::The small regulatory protein Crl activates sigma(S) (RpoS), the stationary-phase and general stress response sigma factor. Crl has been reported to bind sigma(S) in vitro and to facilitate the formation of RNA polymerase holoenzyme. In Salmonella enterica serovar Typhimurium, Crl is required for the development of the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01919-06

    authors: Robbe-Saule V,Lopes MD,Kolb A,Norel F

    更新日期:2007-04-01 00:00:00

  • Variation between pathogenic serovars within Salmonella pathogenicity islands.

    abstract::Although four of the five Salmonella pathogenicity islands (SPIs) have been characterized in detail for Salmonella enterica serovar Typhimurium, and the fifth has been characterized for Salmonella enterica serovar Dublin, there have been limited studies to examine them in detail in a range of pathogenic serovars of S....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.12.3624-3635.2003

    authors: Amavisit P,Lightfoot D,Browning GF,Markham PF

    更新日期:2003-06-01 00:00:00

  • Survey of extrachromosomal DNA found in the filamentous cyanobacteria.

    abstract::Cleared lysates of 13 species of filamentous cyanobacteria were examined for the presence of extrachromosomal DNA by using agarose gel electrophoresis and ethidium bromide staining. Seven of the 13 species contained extrachromosomal covalently closed circular DNA, and all but 1 species contained multiple elements. The...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.1.414-418.1978

    authors: Simon RD

    更新日期:1978-10-01 00:00:00

  • The thioredoxin system of Penicillium chrysogenum and its possible role in penicillin biosynthesis.

    abstract::Penicillium chrysogenum is an important producer of penicillin antibiotics. A key step in their biosynthesis is the oxidative cyclization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to isopenicillin N by the enzyme isopenicillin N synthase (IPNS). bis-ACV, the oxidized disulfide form of ACV is, however, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.4.973-984.1994

    authors: Cohen G,Argaman A,Schreiber R,Mislovati M,Aharonowitz Y

    更新日期:1994-02-01 00:00:00

  • The RNA chaperone Hfq independently coordinates expression of the VirB type IV secretion system and the LuxR-type regulator BabR in Brucella abortus 2308.

    abstract::The type IV secretion system encoded by the virB operon is required for full virulence of Brucella sp., and the present study links the RNA chaperone Hfq to wild-type expression of virB in Brucella abortus 2308. Studies employing virB-lacZ fusions, quantitative reverse transcription-PCR, and immunoblot analysis showed...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05623-11

    authors: Caswell CC,Gaines JM,Roop RM 2nd

    更新日期:2012-01-01 00:00:00

  • Flavoproteins are potential targets for the antibiotic roseoflavin in Escherichia coli.

    abstract::The riboflavin analog roseoflavin is an antibiotic produced by Streptomyces davawensis. Riboflavin transporters are responsible for roseoflavin uptake by target cells. Roseoflavin is converted to the flavin mononucleotide (FMN) analog roseoflavin mononucleotide (RoFMN) by flavokinase and to the flavin adenine dinucleo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00646-13

    authors: Langer S,Hashimoto M,Hobl B,Mathes T,Mack M

    更新日期:2013-09-01 00:00:00

  • Efficient expression of the yeast metallothionein gene in Escherichia coli.

    abstract::The yeast metallothionein gene CUP1 was cloned into a bacterial expression system to achieve efficient, controlled expression of the stable, unprocessed protein product. The Escherichia coli-synthesized yeast metallothionein bound copper, cadmium, and zinc, indicating that the protein was functional. Furthermore, E. c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.1.21-26.1988

    authors: Berka T,Shatzman A,Zimmerman J,Strickler J,Rosenberg M

    更新日期:1988-01-01 00:00:00

  • Allosteric regulation of the glucose:H+ symporter of Lactobacillus brevis: cooperative binding of glucose and HPr(ser-P).

    abstract::Lactobacillus brevis transports glucose and the nonmetabolizable glucose analog 2-deoxyglucose via a proton symport mechanism that is allosterically inhibited by the seryl-phosphorylated derivative of HPr, the small phosphocarrier protein of the phosphotransferase system. We have demonstrate that S46DHPr, a mutant ana...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.7.1900-1902.1995

    authors: Ye JJ,Saier MH Jr

    更新日期:1995-04-01 00:00:00

  • Role of sodium in determining alternate pathways of aerobic citrate catabolism in Aerobacter aerogenes.

    abstract::In contrast to the absolute Na(+) requirement for anaerobic growth of Aerobacter aerogenes on citrate as sole carbon source, aerobic growth of this microorganism did not require the presence of Na(+). However, Na(+) (optimal concentration, 10 mm) did increase the maximal amount of aerobic growth by 60%, even though it...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.99.2.389-394.1969

    authors: O'Brien RW,Stern JR

    更新日期:1969-08-01 00:00:00

  • Spectra of spontaneous growth-dependent and adaptive mutations at ebgR.

    abstract::A comparison of the spectra of spontaneous growth-dependent and adaptive mutations in ebgR shows that both spectra are dominated by insertion sequence (IS)-mediated mutations. The difference between growth-dependent mutations (61% IS mediated) and adaptive mutations (80% IS mediated) is highly significant (P < 0.0001)...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.4.1149-1155.1999

    authors: Hall BG

    更新日期:1999-02-01 00:00:00

  • Physiology of Sporeforming Bacteria Associated with Insects II. Lipids of Vegetative Cells.

    abstract::Lipid composition was studied in two strains each of mid-log phase cells of Bacillus thuringiensis, B. larvae, B. popilliae, B. alvei, and B. lentimorbus. Total lipids varied from 2.5 to 3.5% of the cell dry weight of B. thuringiensis to 4.3 to 5.0% of B. popilliae. Phospholipids in the organisms examined ranged from ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.3.1246-1253.1970

    authors: Bulla LA,Bennett GA,Shotwell OL

    更新日期:1970-12-01 00:00:00