Neurospora mutant exhibiting hyperproduction of amylase and invertase.

abstract：:The nucleotide sequence of a 3.4-kb EcoRI-PstI DNA fragment of Xanthomonas campestris pv. campestris revealed two open reading frames, which were designated xanA and xanB. The genes xanA and xanB encode proteins of 448 amino acids (molecular weight of 48,919) and 466 amino acids (molecular weight of 50,873), respectiv...

journal_title：Journal of bacteriology

authors： Köplin R,Arnold W,Hötte B,Simon R,Wang G,Pühler A

更新日期：1992-01-01 00:00:00

abstract：:PCR-based site-directed mutagenesis has been used to generate 38 alanine-substitution mutations in the C-terminal 41 amino acid residues of LuxR. This region plays a critical role in the mechanism of LuxR-dependent transcriptional activation of the Vibrio fischeri lux operon during quorum sensing. The ability of the v...

journal_title：Journal of bacteriology

authors： Trott AE,Stevens AM

更新日期：2001-01-01 00:00:00

abstract：:Bacillus subtilis mutants deficient in amino acid repression of the histidine utilization (hut) operon were isolated by transposon mutagenesis. Genetic characterization of these mutants indicated that they most likely contained transposon insertions within the codVWXY operon. The codY gene is required for nutritional ...

journal_title：Journal of bacteriology

authors： Fisher SH,Rohrer K,Ferson AE

更新日期：1996-07-01 00:00:00

abstract：:An apparatus has been developed in which bacterial growth can be measured very precisely over short intervals of time. Its precision is presented and used to assess the constancy of growth in batch culture. Under certain conditions, i.e., Luria broth or 0.2% glucose-M9 medium at very low cell densities, the specific g...

journal_title：Journal of bacteriology

authors： Wang CH,Koch AL

更新日期：1978-12-01 00:00:00

abstract：:The development of competence by the dental caries pathogen Streptococcus mutans is mediated primarily through the alternative sigma factor ComX (SigX), which is under the control of multiple regulatory systems and activates the expression of genes involved in DNA uptake and recombination. Here we report that the indu...

journal_title：Journal of bacteriology

authors： Guo Q,Ahn SJ,Kaspar J,Zhou X,Burne RA

更新日期：2014-01-01 00:00:00

abstract：:Expression of virulence factors by Bordetella pertussis is altered by environmental signals (antigenic modulation) and is dependent on an activator encoded by a gene called vir. We have used TnphoA (Tn5 IS50L::phoA) gene fusions to define two sets of genes whose expression is either activated (vag loci) or repressed (...

journal_title：Journal of bacteriology

authors： Knapp S,Mekalanos JJ

更新日期：1988-11-01 00:00:00

abstract：:Pseudomonas aeruginosa XMG, isolated from soil, utilizes lactate. Here we present a 6.45-Mb assembly of its genome sequence. Besides the lactate utilization mechanism of the strain, the genome sequence may also provide other useful information related to P. aeruginosa, such as identifying genes involved in virulence, ...

journal_title：Journal of bacteriology

authors： Gao C,Hu C,Ma C,Su F,Yu H,Jiang T,Dou P,Wang Y,Qin T,Lv M,Xu P

更新日期：2012-09-01 00:00:00

abstract：:Oligoribonuclease, an exoribonuclease specific for small oligoribonucleotides, was initially characterized 20 years ago (S. K. Niyogi and A. K. Datta, J. Biol. Chem. 250:7307-7312, 1975) and shown to be different from RNase II and polynucleotide phosphorylase. Here we demonstrate, using mutant strains and purified enz...

journal_title：Journal of bacteriology

authors： Yu D,Deutscher MP

更新日期：1995-07-01 00:00:00

abstract：:The capsular polysaccharide is a critical virulence factor for group B streptococci associated with human infections, yet little is known about capsule biosynthesis. We detected CMP-Neu5Ac synthetase, the enzyme which activates N-acetylneuraminic acid (Neu5Ac, or sialic acid) for transfer to the nascent capsular polys...

journal_title：Journal of bacteriology

authors： Haft RF,Wessels MR

更新日期：1994-12-01 00:00:00

abstract：:The yeast two-hybrid assay revealed that Bacillus subtilis DnaD, a possible component of the primosome and required for replication initiation, interacted with DnaA and DnaD itself. The mutant DnaD23 was incapable of interacting with DnaA but retained interaction with the wild-type DnaD. These results suggest that int...

journal_title：Journal of bacteriology

authors： Ishigo-Oka D,Ogasawara N,Moriya S

更新日期：2001-03-01 00:00:00

abstract：:Iron-sulfur flavoproteins (ISF) constitute a widespread family of redox-active proteins in anaerobic prokaryotes. Based on sequence homologies, their overall structure is expected to be similar to that of flavodoxins, but in addition to a flavin mononucleotide cofactor they also contain a cubane-type [4Fe:4S] cluster....

journal_title：Journal of bacteriology

authors： Andrade SL,Cruz F,Drennan CL,Ramakrishnan V,Rees DC,Ferry JG,Einsle O

更新日期：2005-06-01 00:00:00

abstract：:Flagellate bacteria such as Escherichia coli and Salmonella enterica serovar Typhimurium typically express 5 to 12 flagellar filaments over their cell surface that rotate in clockwise (CW) and counterclockwise directions. These bacteria modulate their swimming direction towards favorable environments by biasing the di...

journal_title：Journal of bacteriology

authors： Morehouse KA,Goodfellow IG,Sockett RE

更新日期：2005-03-01 00:00:00

abstract：:The sigA and sigB genes of Mycobacterium tuberculosis encode two sigma 70-like sigma factors of RNA polymerase. While transcription of the sigA gene is growth rate independent, sigB transcription is increased during entry into stationary phase. The sigA gene transcription is unresponsive to environmental stress but th...

journal_title：Journal of bacteriology

authors： Hu Y,Coates AR

更新日期：1999-01-01 00:00:00

abstract：:Azotobacter vinelandii produces two detectable catalases during growth on minimal medium. The heat-labile catalase expressed during exponential growth phase was identified as a KatG homologue by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a mixed protein sample. The second catalase was heat resista...

journal_title：Journal of bacteriology

authors： Sandercock JR,Page WJ

更新日期：2008-02-01 00:00:00

abstract：:Growth of Lactococcus lactis in milk depends on the utilization of extracellular peptides. Up to now, oligopeptide uptake was thought to be due only to the ABC transporter Opp. Nevertheless, analysis of several Opp-deficient L. lactis strains revealed the implication of a second oligopeptide ABC transporter, the so-ca...

journal_title：Journal of bacteriology

authors： Lamarque M,Charbonnel P,Aubel D,Piard JC,Atlan D,Juillard V

更新日期：2004-10-01 00:00:00

abstract：:Cells of Pseudomonas stutzeri are naturally transformed by homologous chromosomal DNA; they do not require chemical treatment to become competent. This capacity to undergo natural transformation was found to be shared by the closely related species P. mendocina, P. alcaligenes, and P. pseudoalcaligenes, but was not de...

journal_title：Journal of bacteriology

authors： Carlson CA,Pierson LS,Rosen JJ,Ingraham JL

更新日期：1983-01-01 00:00:00

abstract：:The vitamin requirements of the group Q streptococci (Streptococcus avium) and the established enterococcal species (S. faecalis and S. faecium) paralleled one another, although S. avium did not characteristically require riboflavine or pyridoxal. S. avium was further differentiated in that it required thymine for gro...

journal_title：Journal of bacteriology

authors： Nowlan SS,Deibel RH

更新日期：1967-08-01 00:00:00

abstract：:NifA is the general transcriptional activator of nitrogen fixation genes in diazotrophic bacteria. In Rhizobium leguminosarum bv. viciae UPM791, the nifA gene is part of a gene cluster (orf71 orf79 fixW orf5 fixABCX nifAB) separated by 896 bp from an upstream and divergent truncated duplication of nifH (DeltanifH). Sy...

journal_title：Journal of bacteriology

authors： Martínez M,Palacios JM,Imperial J,Ruiz-Argüeso T

更新日期：2004-10-01 00:00:00

abstract：:Application of in vivo expression technology (IVET) to Yersinia ruckeri, an important fish pathogen, allowed the identification of two adjacent genes that represent a novel bacterial system involved in the uptake and degradation of l-cysteine. Analysis of the translational products of both genes showed permease domain...

journal_title：Journal of bacteriology

authors： Méndez J,Reimundo P,Pérez-Pascual D,Navais R,Gómez E,Guijarro JA

更新日期：2011-02-01 00:00:00

abstract：:The cysteine-rich envelope proteins of the elementary body form of chlamydiae are thought to be located in the outer membrane on the basis of their insolubility in the weak anionic detergent N-lauryl sarcosinate (Sarkosyl). We found, however, that the insolubility of the small (EnvA) and the large (EnvB) cysteine-rich...

journal_title：Journal of bacteriology

authors： Everett KD,Hatch TP

更新日期：1995-02-01 00:00:00

abstract：:The isolation of a new thermophilic bacterium, Thermus aquaticus gen. n. and sp. n., is described. Successful enrichment requires incubation at 70 to 75 C, and the use of nutrient media relatively dilute with respect to the organic components. Strains of T. aquaticus have been isolated from a variety of thermal spring...

journal_title：Journal of bacteriology

authors： Brock TD,Freeze H

更新日期：1969-04-01 00:00:00

abstract：:AdnA is a transcription factor in Pseudomonas fluorescens that affects flagellar synthesis, biofilm formation, and sand adhesion. To identify the AdnA regulon, we used a promoterless Tn5-lacZ element to study the phenotypes of insertion mutants in the presence and absence of AdnA. Of 12,000 insertions, we identified s...

journal_title：Journal of bacteriology

authors： Robleto EA,López-Hernández I,Silby MW,Levy SB

更新日期：2003-01-01 00:00:00

abstract：:The growth of tubercle bacilli in serum samples of untreated animals depends upon the availability of ionic iron which serves as a growth factor in supporting bacillary multiplication. The amount of available iron in serum is determined by the ratio between iron-saturated and iron-free transferrin; a low value for the...

journal_title：Journal of bacteriology

authors： Kochan I,Golden CA,Bukovic JA

更新日期：1969-10-01 00:00:00

abstract：:The tprK gene of Treponema pallidum subsp. pallidum, the causative agent of venereal syphilis, belongs to a 12-member gene family and encodes a protein with a predicted cleavable signal sequence and predicted transmembrane domains. Except for the Nichols type strain, all rabbit-propagated isolates of T. pallidum exami...

journal_title：Journal of bacteriology

authors： LaFond RE,Centurion-Lara A,Godornes C,Rompalo AM,Van Voorhis WC,Lukehart SA

更新日期：2003-11-01 00:00:00

abstract：:The induction of paraffin oxidation in intact cells of Pseudomonas aeruginosa was investigated. Oxidation of (14)C-heptane by cell-free extracts of adapted cells showed that the activity of whole cells is a reliable reflection of the synthesis of the first enzyme in the degradation of n-alkanes. Induction was signific...

journal_title：Journal of bacteriology

authors： van Eyk J,Bartels TJ

更新日期：1968-09-01 00:00:00

abstract：:Davis, Nour A. (University of Lagos Medical School, Lagos, Nigeria), and G. H. G. Davis. Ecology of nasal staphylococci. J. Bacteriol. 89:1163-1168. 1965.-The rate of nasal carriage of Staphylococcus aureus in Nigerian adults (46%) approximates that found in other countries. The rate in infants under 12 months was ca....

journal_title：Journal of bacteriology

authors： DAVIS NA,DAVIS GH

更新日期：1965-05-01 00:00:00

abstract：:Genome sequencing revealed that all six chlamydiae genomes contain three groEL-like genes (groEL1, groEL2, and groEL3). Phylogenetic analysis of groEL1, groEL2, and groEL3 indicates that these genes are likely to have been present in chlamydiae since the beginning of the lineage. Comparison of deduced amino acid seque...

journal_title：Journal of bacteriology

authors： Karunakaran KP,Noguchi Y,Read TD,Cherkasov A,Kwee J,Shen C,Nelson CC,Brunham RC

更新日期：2003-03-01 00:00:00

abstract：UNLABELLED:The airways of patients with cystic fibrosis are colonized with diverse bacterial communities that change dynamically during pediatric years and early adulthood. Staphylococcus aureus is the most prevalent pathogen during early childhood, but during late teens and early adulthood, a shift in microbial compos...

journal_title：Journal of bacteriology

authors： Filkins LM,Graber JA,Olson DG,Dolben EL,Lynd LR,Bhuju S,O'Toole GA

更新日期：2015-07-01 00:00:00

abstract：:NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not a...

journal_title：Journal of bacteriology

authors： Ras J,Van Ophem PW,Reijnders WN,Van Spanning RJ,Duine JA,Stouthamer AH,Harms N

更新日期：1995-01-01 00:00:00

abstract：:top mutations (formerly supX) eliminate DNA topoisomerase I activity and suppress the leu-500 promoter mutation in Salmonella typhimurium (K. M. Overbye, S. K. Basu, and P. Margolin, Cold Spring Harbor Symp. Quant. Biol. 47:785-791, 1983). Sublethal doses of coumermycin which reduce intracellular levels of supercoilin...

journal_title：Journal of bacteriology

authors： Pruss GJ,Drlica K

更新日期：1985-11-01 00:00:00