Abstract:
:The type IV pili of plasmid R64 belonging to the type IVB group are required only for liquid mating. They consist of the major and minor components PilS pilin and PilV adhesin, respectively. PilS pilin is first synthesized as a 22-kDa prepilin from the pilS gene and is then processed to a 19-kDa mature pilin by PilU prepilin peptidase. In a previous genetic analysis, we identified four classes of the pilS mutants (T. Horiuchi and T. Komano, J. Bacteriol. 180:4613-4620, 1998). The products of the class I pilS mutants were not processed by prepilin peptidase; the products of the class II mutants were not secreted; in the class III mutants type IV pili with reduced activities in liquid mating were produced; and in the class IV mutants type IV pili with normal activities were produced. Here, we describe a novel class, class V, of pilS mutants. Mutations in the pilS gene at Gly-56 or Tyr-57 produced type IV pili lacking PilV adhesin, which were inactive in liquid mating. Residues 56 and 57 of PilS pilin are suggested to function as an interface of PilS-PilV interactions.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Shimoda E,Muto T,Horiuchi T,Furuya N,Komano Tdoi
10.1128/JB.01204-07subject
Has Abstractpub_date
2008-02-01 00:00:00pages
1202-8issue
4eissn
0021-9193issn
1098-5530pii
JB.01204-07journal_volume
190pub_type
杂志文章abstract::DNA polymerase activities in fractionated cell extract of Aeropyrum pernix, a hyperthermophilic crenarchaeote, were investigated. Aphidicolin-sensitive (fraction I) and aphidicolin-resistant (fraction II) activities were detected. The activity in fraction I was more heat stable than that in fraction II. Two different ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.19.5984-5992.1999
更新日期:1999-10-01 00:00:00
abstract::In Escherichia coli, repression of the aceBAK operon is mediated by the IclR protein. We used an in vitro oligonucleotide selection technique to determine the consensus recognition sequence for MR. Mutational analysis confirmed the contribution of this sequence to repression in vivo and identified the -35 element of t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.13.3982-3984.1996
更新日期:1996-07-01 00:00:00
abstract::Protein K is an outer membrane protein found in pathogenic encapsulated strains of Escherichia coli. We present evidence here that protein K is structurally and functionally related to the E. coli K-12 porin proteins (OmpF, OmpC, and PhoE). Protein K was found to cross-react with antibody to OmpF protein and to share ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.2.867-872.1983
更新日期:1983-11-01 00:00:00
abstract::In Neurospora crassa, histidine starvation of histidine mutants resulted in derepression of histidine, tryptophan, and arginine biosynthetic enzymes. The same tripartite derepression occurred in wild-type strain 74A when it was grown in medium supplemented with 3-amino-1,2,4-triazole, an inhibitor of histidine biosynt...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.3.893-898.1974
更新日期:1974-09-01 00:00:00
abstract::Seventeen strains of mycoplasmata representing 11 named species were compared serologically by three parameters: growth inhibition on agar, double immunodiffusion, and complement fixation. In growth-inhibition studies, a strain labeled Mycoplasma histotropicum was found related to and perhaps best classified as M. pul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.3.1044-1055.1969
更新日期:1969-06-01 00:00:00
abstract::The first enzymes of the histidine (hut) and proline degradative pathways, histidase and proline oxidase, could not be induced in Bacillus subtilis cells growing in glucose minimal medium containing a mixture of 16 amino acids. Addition of the 16-amino-acid mixture to induced wild-type cells growing in citrate minimal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.4758-4765.1990
更新日期:1990-09-01 00:00:00
abstract::The control of expression of the Bacillus subtilis spoIIA locus was analyzed by titrating gene expression against gene copy number. A plasmid integrated into the B. subtilis chromosome and carrying the spoIIA control region fused to Escherichia coli lacZ was forced to form tandem repeats by the selection of clones tha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1260-1266.1987
更新日期:1987-03-01 00:00:00
abstract::Monobromoacetate (MBA) is toxic for the 1,2-dichloroethane-degrading bacterium Xanthobacter autotrophicus GJ10 at concentrations higher than 5 mM. Mutants which are able to grow on higher concentrations of MBA were isolated and found to overexpress haloacid dehalogenase, which is encoded by the dhlB gene. In mutant GJ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.5.1348-1356.1995
更新日期:1995-03-01 00:00:00
abstract::LamB-LacZ fusion proteins have classically been used in studies of the general secretion pathway of Escherichia coli. Here we describe how increasing signal sequence hydrophobicity routes LamB-LacZ Hyb42-1 to the signal recognition particle (SRP) pathway. Secretion of this hydrophobic fusion variant (H*LamB-LacZ) was ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.19.5697-5705.2003
更新日期:2003-10-01 00:00:00
abstract::A nicotinamide adenine dinucleotide-specific L-(+)-lactate dehydrogenase (LDH) (EC 1.11.27) from Actinomyces viscosus T-6-1600 was purified approximately 110-fold by a combination of diethylaminoethyl-cellulose and 0.5 M Agarose A column chromatography. The ldh was stable at 26 C, but was quite labile at temperatures ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.3.1126-1135.1975
更新日期:1975-06-01 00:00:00
abstract::Energy-coupling factor (ECF) transporters form a large group of vitamin uptake systems in prokaryotes. They are composed of highly diverse, substrate-specific, transmembrane proteins (S units), a ubiquitous transmembrane protein (T unit), and homo- or hetero-oligomeric ABC ATPases. Biotin transporters represent a spec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00350-13
更新日期:2013-09-01 00:00:00
abstract::Antigen 43 has been identified as a unique protein complex in the outer membrane of Escherichia coli. The complex contains two different polypeptides, alpha (Mr, 60,000) and beta (Mr, 53,000), in equal stoichiometry (P. Owen, P. Caffrey, and L.-G. Josefsson, J. Bacteriol. 169:3770-3777, 1987). The alpha subunit was re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.3634-3640.1989
更新日期:1989-07-01 00:00:00
abstract::CRISPR-Cas systems are prokaryotic adaptive immune systems that facilitate protection of bacteria and archaea against infection by external mobile genetic elements. The model pathogen Francisella novicida encodes a CRISPR-Cas12a (FnoCas12a) system and a CRISPR-Cas9 (FnoCas9) system, the latter of which has an addition...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00670-19
更新日期:2020-05-27 00:00:00
abstract::Comprehensive analysis of genome-wide expression patterns during growth of the hyperthermophilic bacterium Thermotoga maritima on 14 monosaccharide and polysaccharide substrates was undertaken with the goal of proposing carbohydrate specificities for transport systems and putative transcriptional regulators. Saccharid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.21.7267-7282.2005
更新日期:2005-11-01 00:00:00
abstract::Pfs expression is required for several metabolic pathways and limits the production of autoinducer-2, a molecule proposed to play a central role in interspecies quorum sensing. The present study reveals physiological conditions and promoter DNA elements that regulate Escherichia coli pfs transcription. Pfs transcripti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00868-06
更新日期:2006-11-01 00:00:00
abstract::Capnocytophaga spp. contain a group of unusual sulfonolipids, called capnoids (W. Godchaux III and E. R. Leadbetter, J. Bacteriol. 144:592-602, 1980). One of these lipids, capnine, is 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid; the others are, apparently, N-acylated versions of capnine. The lipids were foun...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.3.1238-1246.1983
更新日期:1983-03-01 00:00:00
abstract::Escherichia coli O111a is a thermosensitive strain which, when grown at 40 C, accumulates large quantities of intracellular membranes. The ultrastructure of these membranes in cells which have been chemically fixed, embedded, and examined as thin sections has been compared with that of membranes in cells negatively st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.1.433-444.1973
更新日期:1973-01-01 00:00:00
abstract::A superfamily of transport proteins, which includes the lactose permease of Escherichia coli, contains a highly conserved motif, G-X-X-X-D/E-R/K-X-G-R/K-R/K, in the loops that connect transmembrane segments 2 and 3 and transmembrane segments 8 and 9. Previous analysis of this motif in the lactose permease (A. E. Jesse...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.8.2616-2622.1997
更新日期:1997-04-01 00:00:00
abstract::We describe here the identification and characterization of two Listeria monocytogenes (Tn917-LTV3) relA and hpt transposon insertion mutants that were impaired in growth after attachment to a model surface. Both mutants were unable to accumulate (p)ppGpp in response to amino acid starvation, whereas the wild-type str...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.3.621-628.2002
更新日期:2002-02-01 00:00:00
abstract::Multiple promoters drive the expression of the principal cell division gene, ftsZ, in bacterial systems. Primer extension analysis of total RNA from Mycobacterium tuberculosis and a Mycobacterium smegmatis transformant containing 1.117 kb of the upstream region of M. tuberculosis ftsZ and promoter fusion studies ident...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.7.2540-2550.2005
更新日期:2005-04-01 00:00:00
abstract::Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA and ChiB (ArChiA and ArChiB), in response to chitin induction. A single chromosomal DNA fragment containing the genes c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.5.1773-1779.2001
更新日期:2001-03-01 00:00:00
abstract::Repaske, Roy (National Institute of Allergy and Infectious Diseases, Bethesda, Md.). Nutritional requirements for Hydrogenomonas eutropha. J. Bacteriol. 83: 418-422. 1962.-A simple apparatus for the autotrophic cultivation of Hydrogenomonas eutropha in 100-ml shake cultures is described. Nitrogen, in the form of ammon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.2.418-422.1962
更新日期:1962-02-01 00:00:00
abstract::The number and disposition of flagella harbored by eubacteria are regulated by a specific trait successfully maintained over generations. The genes governing the number of flagella in Bacillus subtilis have never been identified, although the ifm locus has long been recognized to influence the motility phenotype of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.15.5356-5366.2005
更新日期:2005-08-01 00:00:00
abstract::Mutations in sulA (sfiA) block the filamentation and death of capR (lon) mutants that occur after treatments that either damage DNA or inhibit DNA replication and thereby induce the SOS response. Previous sulA-lacZ gene fusion studies showed that sulA is transcriptionally regulated by the SOS response system (lexA/rec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.2.551-561.1984
更新日期:1984-05-01 00:00:00
abstract::The global regulator CsrA (carbon storage regulator) is an RNA binding protein that coordinates central carbon metabolism, activates flagellum biosynthesis and motility, and represses biofilm formation in Escherichia coli. CsrA activity is antagonized by the untranslated RNA CsrB, to which it binds and forms a globula...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.18.5130-5140.2002
更新日期:2002-09-01 00:00:00
abstract::Desulfomonile tiedjei DCB-1, a sulfate-reducing bacterium, conserves energy for growth from reductive dehalogenation of 3-chlorobenzoate by an uncharacterized chemiosmotic process. Respiratory electron transport components were examined in D. tiedjei cells grown under conditions for reductive dehalogenation, pyruvate ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.1.40-46.1999
更新日期:1999-01-01 00:00:00
abstract::Reyranella massiliensis is an Alphaproteobacterium member of the class Rhodospirillaceae, growing in amoebae. We sequenced the genome of type strain 521(T). It is composed of a 5,792,218-bp chromosome and encodes 5,675 protein-coding genes and 53 RNA genes, including 3 rRNA genes. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01260-12
更新日期:2012-10-01 00:00:00
abstract::We report the complete and annotated genome sequence of the nonpathogenic Listeria seeligeri SLCC3954 serovar 1/2b type strain harboring the smallest completely sequenced genome of the genus Listeria. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01415-09
更新日期:2010-03-01 00:00:00
abstract::The genetic requirements for the excision repair of thymine glycols, urea residues, and apurinic (AP) sites were examined by measuring the survival in Escherichia coli mutants of phi X174 replicative form (RF) I transfecting DNA containing selectively introduced lesions. phi X RF I DNA containing thymine glycols was i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.8.3359-3366.1988
更新日期:1988-08-01 00:00:00
abstract::The role of the energy status of the yeast cell in the sensitivity of cultures to two yeast toxins was examined by using 12K release from cells as a measure of toxin action. The Saccharomyces cerevisiae killer toxin bound to sensitive cells in the presence of drugs that interfered with the generation or use of energy,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.668-677.1977
更新日期:1977-02-01 00:00:00