Isolation and characterization of cell wall-defective variants of Bacillus subtilis and Bacillus licheniformis.

Abstract:

:Quantitative mass conversion of intact bacterial cells of Bacillus subtilis and B. licheniformis to L-phase variants has been effected after lysozyme treatment. After subculture of the unstable L-phase variants for several months in the presence of methicillin, stable L-phase variants were obtained which grew in the absence of the antibiotic and were then unable to revert to the classical bacterial phase under conditions which gave rise to mass reversion of the protoplasts and unstable L-variants. These stable L-phase variants, which retained many of the physiological properties of the bacterium from which they were derived, were capable of growing exponentially and multiplying in liquid medium. Their morphology and apparent modes of reproduction were consistent with that described for other L-phase variants. The morphological events, as monitored by the electron microscope, of the reversion to the intact bacterial phase of an unstable L-phase variant of B. licheniformis are described.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Wyrick PB,Rogers HJ

doi

10.1128/JB.116.1.456-465.1973

subject

Has Abstract

pub_date

1973-10-01 00:00:00

pages

456-65

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

116

pub_type

杂志文章
  • EPITHELIAL CELL PENETRATION AS AN ESSENTIAL STEP IN THE PATHOGENESIS OF BACILLARY DYSENTERY.

    abstract::LaBrec, Eugene H., Herman Schneider, Thomas J. Magnani, and Samuel B. Formal. Epithelial cell penetration as an essential step in the pathogenesis of bacillary dysentery. J. Bacteriol. 88:1503-1518. 1964.-A parent strain of Shigella flexneri 2a and a colonial mutant derived from it were studied in three animal models....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.5.1503-1518.1964

    authors: Labrec EH,Schneider H,Magnani TJ,Formal SB

    更新日期:1964-11-01 00:00:00

  • A multifunction ABC transporter (Opt) contributes to diversity of peptide uptake specificity within the genus Lactococcus.

    abstract::Growth of Lactococcus lactis in milk depends on the utilization of extracellular peptides. Up to now, oligopeptide uptake was thought to be due only to the ABC transporter Opp. Nevertheless, analysis of several Opp-deficient L. lactis strains revealed the implication of a second oligopeptide ABC transporter, the so-ca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.19.6492-6500.2004

    authors: Lamarque M,Charbonnel P,Aubel D,Piard JC,Atlan D,Juillard V

    更新日期:2004-10-01 00:00:00

  • A distant upstream site involved in the negative regulation of the Escherichia coli ompF gene.

    abstract::The two-component regulatory system, OmpR-EnvZ, of Escherichia coli K-12 regulates the expression of the major outer membrane porin protein, OmpF. OmpR is a DNA-binding protein which acts as both an activator and a repressor to control ompF transcription. In this article, we describe a new OmpR-binding site that is lo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.5.1309-1315.1994

    authors: Huang KJ,Schieberl JL,Igo MM

    更新日期:1994-03-01 00:00:00

  • A novel cdsAB operon is involved in the uptake of L-cysteine and participates in the pathogenesis of Yersinia ruckeri.

    abstract::Application of in vivo expression technology (IVET) to Yersinia ruckeri, an important fish pathogen, allowed the identification of two adjacent genes that represent a novel bacterial system involved in the uptake and degradation of l-cysteine. Analysis of the translational products of both genes showed permease domain...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01058-10

    authors: Méndez J,Reimundo P,Pérez-Pascual D,Navais R,Gómez E,Guijarro JA

    更新日期:2011-02-01 00:00:00

  • Perturbation of the oxidizing environment of the periplasm stimulates the PhoQ/PhoP system in Escherichia coli.

    abstract::The PhoQ/PhoP two-component system is repressed by divalent cations, such as Mg(2+) and Ca(2+), in the growth medium and stimulated by low pH and certain cationic antimicrobial peptides. In Escherichia coli, it was recently shown that the histidine kinase PhoQ is also modulated by at least two additional factors, the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06055-11

    authors: Lippa AM,Goulian M

    更新日期:2012-03-01 00:00:00

  • Differential depression of staphylococcal plasmid and chromosomal penicillinase genes by a class of unlinked chromosomal mutations (R2-).

    abstract::We described previously a class of mutations (phenotypic designation R2(-)) in Staphylococcus aureus that were unlinked to penicillinase genes but nevertheless imposed constitutive synthesis of this enzyme upon wild-type plasmids. The plasmids affected by these mutations have been found to possess the same maintenance...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.103.3.616-621.1970

    authors: Cohen S,Vernon EG,Sweeney HM

    更新日期:1970-09-01 00:00:00

  • Growth-linked instability of a mutant valyl-transfer ribonucleic acid synthetase in Escherichia coli.

    abstract::The valyl-transfer ribonucleic acid (tRNA) synthetase of Escherichia coli strain NP2907, previously described as having an elevated K(m) for adenosine triphosphate and reduced stability in vitro compared to the wild type, was found to be conditionally thermolabile in vivo. The rate of inactivation of this enzyme at a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.1.315-325.1972

    authors: Anderson JJ,Neidhardt FC

    更新日期:1972-01-01 00:00:00

  • Identification of an essential gene of Listeria monocytogenes involved in teichoic acid biogenesis.

    abstract::Listeria monocytogenes is a facultative intracellular gram-positive bacterium responsible for severe opportunistic infections in humans and animals. We had previously identified a gene encoding a putative UDP-N-acetylglucosamine 2-epimerase, a precursor of the teichoic acid linkage unit, in the genome of L monocytogen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00771-06

    authors: Dubail I,Bigot A,Lazarevic V,Soldo B,Euphrasie D,Dupuis M,Charbit A

    更新日期:2006-09-01 00:00:00

  • Sequence of xynC and properties of XynC, a major component of the Clostridium thermocellum cellulosome.

    abstract::The nucleotide sequence of the Clostridium thermocellum F1 xynC gene, which encodes the xylanase XynC, consists of 1,857 bp and encodes a protein of 619 amino acids with a molecular weight of 69,517. XynC contains a typical N-terminal signal peptide of 32 amino acid residues, followed by a 165-amino-acid sequence whic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.13.4246-4253.1997

    authors: Hayashi H,Takagi KI,Fukumura M,Kimura T,Karita S,Sakka K,Ohmiya K

    更新日期:1997-07-01 00:00:00

  • Cyclic adenosine 3',5'-monophosphate-mediated hyperinduction of araBAD and lacZYA expression in a crp mutant of Escherichia coli K-12.

    abstract::A spontaneous lac+ revertant of an adenylate cyclase deletion strain of Escherichia coli K-12 was isolated and characterized. This revertant, designated strain KC20, exhibited a pleiotropic suppression of the adenylate cyclase defect, with the crp locus being the site of the suppressor mutation. Cyclic adenosine 3',5'...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.147.2.500-508.1981

    authors: Bankaitis VA,Kline EL

    更新日期:1981-08-01 00:00:00

  • Direct comparison of the subtilisin-like intracellular protease of Bacillus licheniformis with the homologous enzymes of Bacillus subtilis.

    abstract::Intracellular serine proteases of Bacillus licheniformis and Bacillus subtilis are closely related. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.2.1017-1019.1979

    authors: Strongin AY,Abramov ZT,Yaroslavtseva NG,Baratova LA,Shaginyan KA,Belyanova LP,Stepanov VM

    更新日期:1979-02-01 00:00:00

  • Tn5401, a new class II transposable element from Bacillus thuringiensis.

    abstract::A new class II (Tn3-like) transposable element, designated Tn5401, was recovered from a sporulation-deficient variant of Bacillus thuringiensis subsp. morrisoni EG2158 following its insertion into a recombinant plasmid. Sequence analysis of the insert revealed a 4,837-bp transposon with two large open reading frames, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.10.2835-2845.1994

    authors: Baum JA

    更新日期:1994-05-01 00:00:00

  • Lysogenic conversion of Salmonella typhimurium bacteriophages A3 and A4 consists of O-acetylation of rhamnose of the repeating unit of the O-antigenic polysaccharide chain.

    abstract::Lysogenization of Salmonella typhimurium with either of the bacteriophages A3 and A4 results in O-acetylation of the L-rhamnose residues of the O-polysaccharide chain of the lipopolysaccharide of the bacterial cell envelope. The O-acetyl group is found on both O-2 and O-3 of the L-rhamnosyl residues. This lysogenic co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.3.1003-1009.1987

    authors: Wollin R,Stocker BA,Lindberg AA

    更新日期:1987-03-01 00:00:00

  • Genetic characterization of the metK locus in Escherichia coli K-12.

    abstract::Three independently isolated metK mutants have been shown to have leisions lying between speB and glc near 57 min on the Escherichia coli chromosome. Two deletions result in a lack of the metC gene product but neither extends into the metK glc region. The three metK mutations are recessive to the wild-type allele carr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.3.1144-1152.1975

    authors: Hunter JS,Greene RC,Su CH

    更新日期:1975-06-01 00:00:00

  • Expression of a mutation affecting F incompatibility in the integrated but not the autonomous state of F.

    abstract::Previously we have described a mutant Hfr strain in which incompatibility between the integrated F factor and an autonomous F-prime (F') factor was abolished. The mutation (inc) was located in the integrated F factor. F-prime factors isolated from the mutant Hfr strain have the same incompatibility behavior as those i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.1.348-353.1976

    authors: Pfister A,DeVries JK,Maas WK

    更新日期:1976-07-01 00:00:00

  • Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence.

    abstract::The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express higher levels of antibioti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.1.40-46.1990

    authors: Seifert HS,Ajioka RS,Paruchuri D,Heffron F,So M

    更新日期:1990-01-01 00:00:00

  • Identification of a methyl-specific restriction system mediated by a conjugative element from Streptomyces bambergiensis.

    abstract::pBL2 was identified genetically but not physically in Streptomyces lividans after its mating with S. bambergiensis. During conjugation, pBL2 was transferred at high frequency to S. lividans and S. coelicolor. pBL2.1 DNA isolated from S. coelicolor exconjugants as a circular plasmid was shown to derive from the genome ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.16.4809-4812.1995

    authors: Zotchev SB,Schrempf H,Hutchinson CR

    更新日期:1995-08-01 00:00:00

  • FORMATE-PYRUVATE EXCHANGE REACTION IN STREPTOCOCCUS FAECALIS. I. FACTOR REQUIREMENT FOR INTACT CELLS.

    abstract::Wood, N. P. (A. & M. College of Texas, College Station), and D. J. O'Kane. Formate-pyruvate exchange reaction in Streptococcus faecalis. I. Factor requirement for whole cells. J. Bacteriol. 87:97-103. 1964.-A factor present in plant and animal sources was found necessary for the incorporation of formate-C(14) into pyr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.1.97-103.1964

    authors: WOOD NP,O'KANE DJ

    更新日期:1964-01-01 00:00:00

  • Regulation of glutamine synthetase. VI. Interactions of inhibitors for Bacillus licheniformis glutamine synthetase.

    abstract::The relationships of five feedback inhibitors for the Bacillus licheniformis glutamine synthetase were investigated. The inhibitors were distinguishable by differences in their competitive relationship for the substrates of the enzyme. Mixtures of l-glutamine and adenosine-5'-monophosphate (AMP) or histidine and AMP c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.4.1016-1024.1967

    authors: Hubbard JS,Stadtman ER

    更新日期:1967-10-01 00:00:00

  • ArcB1, a homolog of Escherichia coli ArcB, regulates dimethyl sulfoxide reduction in Shewanella oneidensis MR-1.

    abstract::Shewanella oneidensis is a metal reducer that uses the cyclic AMP receptor protein, CRP, to regulate anaerobic respiration. In addition, ArcA(So) is required for anaerobic growth with dimethyl sulfoxide (DMSO) and plays a role in aerobic respiration. The sensor kinase that activates ArcA(So) in S. oneidensis is not kn...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01695-09

    authors: Shroff NP,Charania MA,Saffarini DA

    更新日期:2010-06-01 00:00:00

  • ORGANIC REQUIREMENTS AND SYNTHETIC MEDIA FOR GROWTH OF THE MYXOMYCETE PHYSARUM POLYCEPHALUM.

    abstract::Daniel, John W. (University of Wisconsin, Madison), Karlee L. Babcock, Alice H. Sievert, and Harold P. Rusch. Organic requirements and synthetic media for growth of the myxomycete Physarum polycephalum. J. Bacteriol. 86:324-331. 1963.-An isolate of Physarum polycephalum was maintained in submersed pure culture in a co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.2.324-331.1963

    authors: DANIEL JW,BABCOCK KL,SIEVERT AH,RUSCH HP

    更新日期:1963-08-01 00:00:00

  • The recombinase IntA is required for excision of esp-containing ICEEfm1 in Enterococcus faecium.

    abstract::Comparative genome analysis of Enterococcus faecium recently revealed that a genomic island containing the esp gene, referred to as the esp-containing pathogenicity island (esp PAI), can be transferred by conjugation and contains a partial Tn916-like element and an integrase gene, intA. Here, we characterize the role ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00952-10

    authors: Top J,Sinnige JC,Majoor EA,Bonten MJ,Willems RJ,van Schaik W

    更新日期:2011-02-01 00:00:00

  • Oxygen-dependent regulation of the central pathway for the anaerobic catabolism of aromatic compounds in Azoarcus sp. strain CIB.

    abstract::The role of oxygen in the transcriptional regulation of the PN promoter that controls the bzd operon involved in the anaerobic catabolism of benzoate in the denitrifying Azoarcus sp. strain CIB has been investigated. In vivo experiments using PN::lacZ translational fusions, in both Azoarcus sp. strain CIB and Escheric...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.7.2343-2354.2006

    authors: Durante-Rodríguez G,Zamarro MT,García JL,Díaz E,Carmona M

    更新日期:2006-04-01 00:00:00

  • Characterization of a Bacteroides mobilizable transposon, NBU2, which carries a functional lincomycin resistance gene.

    abstract::The mobilizable Bacteroides element NBU2 (11 kbp) was found originally in two Bacteroides clinical isolates, Bacteroides fragilis ERL and B. thetaiotaomicron DOT. At first, NBU2 appeared to be very similar to another mobilizable Bacteroides element, NBU1, in a 2.5-kbp internal region, but further examination of the fu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.12.3559-3571.2000

    authors: Wang J,Shoemaker NB,Wang GR,Salyers AA

    更新日期:2000-06-01 00:00:00

  • Selective plasmid transduction in Bacillus pumilus.

    abstract::The inducible temperate bacteriophage phi75 and a clear-plaque-forming variant, phi75C1, mediated transduction of a 4.4 X 10(6)-dalton multicopy Bacillus pumilus plasmid, pPL10, at frequencies of 10(-5) to 10(-6) transductants per plaque-forming unit. phi75- and phi75C1-mediated transduction of several chromosome mark...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.131.3.1029-1032.1977

    authors: Bramucci MG,Lovett PS

    更新日期:1977-09-01 00:00:00

  • Repressor mutations in the marRAB operon that activate oxidative stress genes and multiple antibiotic resistance in Escherichia coli.

    abstract::Resistance to multiple antibiotics and certain oxidative stress compounds was conferred by three independently selected mutations (marR1, soxQ1, and cfxB1) that mapped to 34 min on the Escherichia coli chromosome. Mutations at this locus can activate the marRAB operon, in which marR encodes a putative repressor of mar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.1.143-148.1994

    authors: Ariza RR,Cohen SP,Bachhawat N,Levy SB,Demple B

    更新日期:1994-01-01 00:00:00

  • Repair and antirepair DNA helicases in Helicobacter pylori.

    abstract::Orthologs of RecG and RuvABC are highly conserved among prokaryotes; in Escherichia coli, they participate in independent pathways that branch migrate Holliday junctions during recombinational DNA repair. RecG also has been shown to directly convert stalled replication forks into Holliday junctions. The bacterium Heli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01848-07

    authors: Kang J,Blaser MJ

    更新日期:2008-06-01 00:00:00

  • Genome-based analysis and gene dosage studies provide new insight into 3-hydroxy-4-methylvalerate biosynthesis in Ralstonia eutropha.

    abstract::Recombinant Ralstonia eutropha strain PHB(-)4 expressing the broad-substrate-specificity polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas sp. strain 61-3 (PhaC1Ps) synthesizes a PHA copolymer containing the branched side-chain unit 3-hydroxy-4-methylvalerate (3H4MV), which has a carbon backbone identical to that...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02474-14

    authors: Saika A,Ushimaru K,Mizuno S,Tsuge T

    更新日期:2015-04-01 00:00:00

  • Genetic evidence that the XylS regulator of the Pseudomonas TOL meta operon controls the Pm promoter through weak DNA-protein interactions.

    abstract::The activation of the Pm promoter of the meta operon of the TOL plasmid of Pseudomonas putida by its cognate XylS activator protein in the presence and absence of benzoate inducers has been examined in specialized Escherichia coli strains carrying Pm-lacZ fusions and the xylS gene in different configurations in which ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.11.3171-3176.1994

    authors: Kessler B,Herrero M,Timmis KN,de Lorenzo V

    更新日期:1994-06-01 00:00:00

  • A global investigation of the Bacillus subtilis iron-sparing response identifies major changes in metabolism.

    abstract::The Bacillus subtilis ferric uptake regulator (Fur) protein is the major sensor of cellular iron status. When iron is limiting for growth, derepression of the Fur regulon increases the cellular capacity for iron uptake and mobilizes an iron-sparing response mediated in large part by a small noncoding RNA named FsrA. F...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05990-11

    authors: Smaldone GT,Revelles O,Gaballa A,Sauer U,Antelmann H,Helmann JD

    更新日期:2012-05-01 00:00:00