The recombinase IntA is required for excision of esp-containing ICEEfm1 in Enterococcus faecium.

Abstract:

:Comparative genome analysis of Enterococcus faecium recently revealed that a genomic island containing the esp gene, referred to as the esp-containing pathogenicity island (esp PAI), can be transferred by conjugation and contains a partial Tn916-like element and an integrase gene, intA. Here, we characterize the role of intA in the excision of the esp PAI. An intA insertion-deletion mutant in E. faecium E1162 (E1162ΔintA) was constructed and in trans complemented with wild-type intA (E1162ΔintA::pEF30). Circular intermediates (CI) of excised esp PAI were determined using inverse PCR analysis on purified chromosomal DNA from strains E1162, E1162Δesp, E1162ΔintA, and E1162ΔintA::pEF30. In E1162 and E1162Δesp, CI of the esp PAI were detected. No CI were detected in E1162ΔintA, while in the complemented strain E1162ΔintA::pEF30 CI formation was restored, indicating that intA is essential for excision and subsequent mobilization of the esp-containing genomic island in E. faecium. Based on the fact that this island can be mobilized and is self-transmissible, we propose to change the name of the esp PAI to ICEEfm1.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Top J,Sinnige JC,Majoor EA,Bonten MJ,Willems RJ,van Schaik W

doi

10.1128/JB.00952-10

subject

Has Abstract

pub_date

2011-02-01 00:00:00

pages

1003-6

issue

4

eissn

0021-9193

issn

1098-5530

pii

JB.00952-10

journal_volume

193

pub_type

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