Phenotypic suppression of DNA gyrase deficiencies by a deletion lowering the gene dosage of a major tRNA in Salmonella typhimurium.

Abstract:

:One of the pleiotropic phenotypes of mutations affecting DNA gyrase activity in Salmonella typhimurium is the constitutive deattenuation of the histidine operon. In the present work, we isolated and characterized a suppressor mutation which restores his attenuation in the presence of a defective gyrase. Such a suppressor, initially named sgdA1 (for suppressor gyrase deficiency), was found to correct additional phenotypes associated with defective gyrase function. These include the aberrant nucleoid partitioning of a gyrB mutant and the conditional lethality of a gyrA mutation. Furthermore, the sgdA1 mutation was found to confer low-level resistance to nalidixic acid. The last phenotype permitted isolation of a number of additional sgdA mutants. Genetic analysis established the recessive character of these alleles as well as the position of the sgdA locus at 57 U on the Salmonella genetic map. All of the sgdA mutants result from the same molecular event: a deletion removing three of the four tandemly repeated copies of argV, the gene which specifies tRNA(2Arg), the major arginine isoacceptor tRNA. These findings, combined with the observation of some Sgd-like phenotypes in a tRNA modification mutant (hisT mutant), lead us to propose that protein synthesis contributes, directly or indirectly, to the pathology of gyrase alterations in growing bacteria. We discuss plausible mechanisms which may be responsible for these effects.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Blanc-Potard AB,Bossi L

doi

10.1128/jb.176.8.2216-2226.1994

subject

Has Abstract

pub_date

1994-04-01 00:00:00

pages

2216-26

issue

8

eissn

0021-9193

issn

1098-5530

journal_volume

176

pub_type

杂志文章
  • Conditional synthesis and utilization of 1,5-anhydroglucitol in Escherichia coli.

    abstract::A cyclic polyol, 1,5-anhydro-D-glucitol (AG), is widely detected in most organisms, although little is known about its metabolism and physiological roles. The present study demonstrates the synthesis of AG in Escherichia coli C600. The major portion of the synthesized AG was indicated to be derived from glucose retain...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.22.7138-7141.1993

    authors: Shiga Y,Mizuno H,Akanuma H

    更新日期:1993-11-01 00:00:00

  • Artificial septal targeting of Bacillus subtilis cell division proteins in Escherichia coli: an interspecies approach to the study of protein-protein interactions in multiprotein complexes.

    abstract::Bacterial cell division is mediated by a set of proteins that assemble to form a large multiprotein complex called the divisome. Recent studies in Bacillus subtilis and Escherichia coli indicate that cell division proteins are involved in multiple cooperative binding interactions, thus presenting a technical challenge...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00462-08

    authors: Robichon C,King GF,Goehring NW,Beckwith J

    更新日期:2008-09-01 00:00:00

  • Mutations in ampG or ampD affect peptidoglycan fragment release from Neisseria gonorrhoeae.

    abstract::Neisseria gonorrhoeae releases peptidoglycan fragments during growth. The majority of the fragments released are peptidoglycan monomers, molecules known to increase pathogenesis through the induction of proinflammatory cytokines and responsible for the killing of ciliated epithelial cells. In other gram-negative bacte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01194-07

    authors: Garcia DL,Dillard JP

    更新日期:2008-06-01 00:00:00

  • Replication of plasmid RK2 in vitro by a DNA-membrane complex: evidence for initiation of replication and its coupling to transcription and translation.

    abstract::The following results with an in vitro replication system utilizing a plasmid RK2 DNA-membrane complex indicate that the essential trfA-encoded replication protein of RK2 is present and active in the complex. (i) A complex extracted from a conditional replication mutant of RK2, which contains a temperature-sensitive m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.1.319-326.1986

    authors: Kornacki JA,Firshein W

    更新日期:1986-07-01 00:00:00

  • Subcellular localization and immunological detection of proteins encoded by the vir locus of Bordetella pertussis.

    abstract::The DNA sequence of the central regulatory locus vir of Bordetella pertussis predicts that three gene products, BvgA, BvgB, and BvgC, are encoded. Features of the predicted primary structures of these proteins and their homology to other two-component systems suggest that BvgA is located in the cytoplasm, BvgB is loca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.14.4288-4296.1991

    authors: Stibitz S,Yang MS

    更新日期:1991-07-01 00:00:00

  • Alteration of tryptophan-mediated regulation in Neurospora crassa by indoleglycerol phosphate.

    abstract::The accumulation of imidazoleglycerol phosphate during growth of Neurospora crassa in the presence of 3-amino-1,2,4-triazole was found to cause derepression of tryptophan synthetase and to inhibit the induction of kynureninase. Accumulation of indoleglycerol phosphate in response to growth in the presence of indole ac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.5.1608-1614.1968

    authors: Turner JR,Matchett WH

    更新日期:1968-05-01 00:00:00

  • Identification and expression of genes narL and narX of the nar (nitrate reductase) locus in Escherichia coli K-12.

    abstract::Previous studies have shown that narL+ is required for nitrate induction of nitrate reductase synthesis and for nitrate inhibition of fumarate reductase synthesis in Escherichia coli. We cloned narL on a 5.1-kilobase HindIII fragment. Our clone also contained a previously unidentified gene, which we propose to designa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.4.1589-1597.1988

    authors: Stewart V,Parales J Jr

    更新日期:1988-04-01 00:00:00

  • Difference between manganese ion requirements of pediococci and enterococci.

    abstract::For maximal turbidity and dissimilatory activity, three strains of pediococci required the addition of Mn(2+) to Rogosa basal medium. Twenty-two strains of enterococci proved indifferent to this supplement. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.1.627-628.1972

    authors: Efthymiou CJ,Joseph SW

    更新日期:1972-10-01 00:00:00

  • Lactate metabolism by Veillonella parvula.

    abstract::A strain of Veillonella parvula M4, which grows readily in lactate broth without a requirement for carbon dioxide, has been isolated from the oral cavity. Anaerobic, washed cells of this organism fermented sodium lactate to the following products (moles/100 moles of lactate): propionate, 66; acetate, 40; carbon dioxid...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.3.999-1005.1971

    authors: Ng SK,Hamilton IR

    更新日期:1971-03-01 00:00:00

  • Specificity and mechanism of tetracycline resistance in a multiple drug resistant strain of Escherichia coli.

    abstract::Izaki, Kazuo (University of Tokyo, Tokyo, Japan), Kan Kiuchi, and Kei Arima. Specificity and mechanism of tetracycline resistance in a multiple drug resistant strain of Escherichia coli. J. Bacteriol. 91:628-633. 1966.-A decrease in the uptake of tetracycline occurred concurrently with a rise in the level of resistanc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.2.628-633.1966

    authors: Izaki K,Kiuchi K,Arima K

    更新日期:1966-02-01 00:00:00

  • Complementation analysis of the cold-sensitive phenotype of the Escherichia coli csdA deletion strain.

    abstract::The cold shock response of Escherichia coli is elicited by downshift of temperature from 37 degrees C to 15 degrees C and is characterized by induction of several cold shock proteins, including CsdA, during the acclimation phase. CsdA, a DEAD-box protein, has been proposed to participate in a variety of processes, suc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00655-07

    authors: Awano N,Xu C,Ke H,Inoue K,Inouye M,Phadtare S

    更新日期:2007-08-01 00:00:00

  • Escherichia coli fimbriae recognizing sialyl galactosides.

    abstract::Fimbriae recognizing sialyl galactosides (S fimbriae) were purified from an Escherichia coli strain. The S fimbriae were morphologically identical to type 1 and P fimbriae of E. coli and showed a hemagglutination that was abolished when erythrocytes were treated with neuraminidase. Hemagglutination by the purified fim...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.2.762-766.1984

    authors: Korhonen TK,Väisänen-Rhen V,Rhen M,Pere A,Parkkinen J,Finne J

    更新日期:1984-08-01 00:00:00

  • Multiple mechanisms are used for growth rate and stringent control of leuV transcriptional initiation in Escherichia coli.

    abstract::Expression of the Escherichia coli leuV operon, which contains three tRNA(1)(Leu) genes, is regulated by several mechanisms including growth-rate-dependent control (GRDC) and stringent control (SC). Structural variants of the leuV promoter which differentially affect these regulatory responses have been identified, su...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.18.5771-5782.1999

    authors: Pokholok DK,Redlak M,Turnbough CL Jr,Dylla S,Holmes WM

    更新日期:1999-09-01 00:00:00

  • Polyamine transport and role of potE in response to osmotic stress in Escherichia coli.

    abstract::When transport of polyamines in Escherichia coli was examined, putrescine excretion was observed under two different physiological conditions: (i) strictly correlated to growth and (ii) following a hyperosmotic shock. Spermidine was not excreted. Characterization of a deletion mutant showed that PotE is not involved i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.21.6247-6249.2000

    authors: Schiller D,Kruse D,Kneifel H,Krämer R,Burkovski A

    更新日期:2000-11-01 00:00:00

  • Effects of selected inhibitors on electron transport in Neisseria gonorrhoeae.

    abstract::The electron transport system of Neisseria gonorrhoeae was partially characterized by using spectrophotometric, spectroscopic, and oxygen consumption measurements. The effects of selected electron transport inhibitors (amytal, rotenone, 2-heptyl-4-hydroxyquinoline, antimycin A1, and potassium cyanide [KCN]) on electro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.2.537-545.1978

    authors: Kenimer EA,Lapp DF

    更新日期:1978-05-01 00:00:00

  • Identification of the PhoB Regulon and Role of PhoU in the Phosphate Starvation Response of Caulobacter crescentus.

    abstract:UNLABELLED:An ability to sense and respond to changes in extracellular phosphate is critical for the survival of most bacteria. For Caulobacter crescentus, which typically lives in phosphate-limited environments, this process is especially crucial. Like many bacteria, Caulobacter responds to phosphate limitation throug...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00658-15

    authors: Lubin EA,Henry JT,Fiebig A,Crosson S,Laub MT

    更新日期:2015-10-19 00:00:00

  • Consequences of glycerol deprivation on the synthesis of membrane components in a glycerol auxotroph of Staphylococcus aureus.

    abstract::In a glycerol auxotroph of Staphylococcus aureus, the deprivation of glycerol affected the formation of certain membrane components. (i) There was synthesis of fatty acids at the predeprivation rate even though the fatty acids synthesized accumulated as free fatty acids rather than as esterified fatty acids; (ii) ther...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.1.413-420.1972

    authors: Ray PH,Lillich TT,White DC

    更新日期:1972-10-01 00:00:00

  • The pinholin of lambdoid phage 21: control of lysis by membrane depolarization.

    abstract::The phage 21 holin, S(21), forms small membrane holes that depolarize the membrane and is designated as a pinholin, as opposed to large-hole-forming holins, like S(lambda). Pinholins require secreted SAR endolysins, a pairing that may represent an intermediate in the evolution of canonical holin-endolysin systems. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00847-07

    authors: Park T,Struck DK,Dankenbring CA,Young R

    更新日期:2007-12-01 00:00:00

  • Central role of the early colonizer Veillonella sp. in establishing multispecies biofilm communities with initial, middle, and late colonizers of enamel.

    abstract::Human dental biofilm communities comprise several species, which can interact cooperatively or competitively. Bacterial interactions influence biofilm formation, metabolic changes, and physiological function of the community. Lactic acid, a common metabolite of oral bacteria, was measured in the flow cell effluent of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01631-09

    authors: Periasamy S,Kolenbrander PE

    更新日期:2010-06-01 00:00:00

  • Degradation of pufLMX mRNA in Rhodobacter capsulatus is initiated by nonrandom endonucleolytic cleavage.

    abstract::Differential expression of genes within the puf photosynthesis operon of Rhodobacter capsulatus is achieved primarily through marked segmental differences in stability within the polycistronic puf operon transcripts. The comparatively stable pufBA segment of these transcripts outlives the labile pufLMX segment and acc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.8.4578-4586.1990

    authors: Chen CY,Belasco JG

    更新日期:1990-08-01 00:00:00

  • Tra proteins characteristic of F-like type IV secretion systems constitute an interaction group by yeast two-hybrid analysis.

    abstract::Using yeast two-hybrid screens, we have defined an interaction group of six Tra proteins encoded by the F plasmid and required by F(+) cells to elaborate F pili. The six proteins are TraH, TraF, TraW, TraU, TrbI, and TrbB. Except for TrbI, these proteins were all identified as hallmarks of F-like type IV secretion sys...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.16.5480-5485.2004

    authors: Harris RL,Silverman PM

    更新日期:2004-08-01 00:00:00

  • Purification of penicillin-binding protein 2 of Escherichia coli.

    abstract::Penicillin-binding protein 2 (PBP-2) of Escherichia coli K-12 was purified by covalent affinity chromatography using 6-aminopenicillanic acid covalently coupled to carboxymethyl-Sepharose (6-APA-CM-Sepharose). Purification of PBP-2 was accomplished by prebinding the methoxy cephalosporin, cefoxitin, to the Triton X-10...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.1.398-403.1981

    authors: Curtis SJ,Strominger JL

    更新日期:1981-01-01 00:00:00

  • Identification and characterization of the nifV-nifZ-nifT gene region from the filamentous cyanobacterium Anabaena sp. strain PCC 7120.

    abstract::The nifV and leuA genes, which encode homocitrate synthase and alpha-isopropylmalate synthase, respectively, were cloned from the filamentous cyanobacterium Anabaena sp. strain PCC 7120 by a PCR-based strategy. Since the N-terminal parts of NifV and LeuA from other bacteria are highly similar to each other, a single p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.9.2930-2937.1997

    authors: Stricker O,Masepohl B,Klipp W,Böhme H

    更新日期:1997-05-01 00:00:00

  • A functional leuABCD operon is required for leucine synthesis by the tyrosine-repressible transaminase in Escherichia coli K-12.

    abstract::In Escherichia coli K-12, two enzymes, encoded by ilvE and tyrB, catalyze the amination of 2-ketoisocaproate (2-KIC) to form leucine. Although leucine-requiring derivatives of an ilvE strain that are unable to grow on 2-KIC were expected to have mutations only in tyrB, mapping studies showed that one such mutation was...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.12.3864-3871.1991

    authors: Vartak NB,Liu L,Wang BM,Berg CM

    更新日期:1991-06-01 00:00:00

  • Structure and Mutational Analyses of Escherichia coli ZapD Reveal Charged Residues Involved in FtsZ Filament Bundling.

    abstract:UNLABELLED:Bacterial cell division is an essential and highly coordinated process. It requires the polymerization of the tubulin homologue FtsZ to form a dynamic ring (Z-ring) at midcell. Z-ring formation relies on a group of FtsZ-associated proteins (Zap) for stability throughout the process of division. In Escherichi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00969-15

    authors: Roach EJ,Wroblewski C,Seidel L,Berezuk AM,Brewer D,Kimber MS,Khursigara CM

    更新日期:2016-05-13 00:00:00

  • Nuclear deoxyribonucleic acid-dependent ribonucleic acid polymerases from Saccharomyces cerevisiae.

    abstract::Two deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerases (I, II) have been solubilized from isolated Saccharomyces cerevisiae nuclei. The enzymes can be separated by chromatography on O-diethylaminoethyl Sephadex. Both enzymes are active with high-molecular-weight nuclear yeast DNA, although RNA po...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.1.1-6.1973

    authors: Sebastian J,Bhargava MM,Halvorson HO

    更新日期:1973-04-01 00:00:00

  • Occurrence of pili on and adhesive properties of Haemophilus parainfluenzae.

    abstract::Of 20 clinical isolates of Haemophilus parainfluenzae, 13 produced a mannose-resistant hemagglutinin that agglutinated erythrocytes from chickens, horses, rabbits, and sheep. Examination with the electron microscope showed that only strain HR-885 was pilate. Grown in static liquid culture, the 12 hemagglutinating, non...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.2.1075-1078.1981

    authors: Kahn ME,Gromkova R

    更新日期:1981-02-01 00:00:00

  • DNA binding sites of the LysR-type regulator GcvA in the gcv and gcvA control regions of Escherichia coli.

    abstract::The GcvA protein is a LysR family regulatory protein necessary for both activation and repression of the Escherichia coli glycine cleavage enzyme operon (gcv) and negative regulation of gcvA. Gel shift assays indicated that overexpressed GcvA in crude extracts is capable of binding specifically to DNA containing the g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.17.4940-4946.1995

    authors: Wilson RL,Urbanowski ML,Stauffer GV

    更新日期:1995-09-01 00:00:00

  • Passive cutaneous anaphylaxis with antigens from Coxiella burneti.

    abstract::Passive cutaneous anaphylaxis (PCA) was produced in guinea pigs sensitized with guinea pig Coxiella burneti phase I-II antiserum and challenged with dimethylsulfoxide- or trichloroacetic acid-soluble extracts from phase I cells. The PCA reaction could not be induced by whole or mechanically disrupted phase I or phase ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.5.1580-1586.1968

    authors: Peacock M,Munoz J,Tallent GL,Ormsbee RA

    更新日期:1968-05-01 00:00:00

  • Sorting out bacterial viability with optical tweezers.

    abstract::We have developed a method, using laser, optical tweezers and direct microscopic analysis of reproductive potential and membrane integrity, to assess single-cell viability in a stationary-phase Escherichia coli population. It is demonstrated here that a reduction in cell integrity, determined by using the fluorescent ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.19.5551-5555.2000

    authors: Ericsson M,Hanstorp D,Hagberg P,Enger J,Nyström T

    更新日期:2000-10-01 00:00:00