Isolation from Pasteurella multocida of a lipopolysaccharide antigen with immunizing and toxic properties.

Abstract:

:A heat-stable, particulate, lipopolysaccharide-protein antigenic complex has been isolated from a virulent, encapsulated strain of Pasteurella multocida by extraction with cold, formalinized saline, and centrifugation at 105,000 x g. The original bacterial culture had been obtained from a bison that died of hemorrhagic septicemia, an infectious disease of cattle and buffalo. Injection of fractional milligram amounts of the antigen into mice, rabbits, and calves produced toxic reactions which frequently resulted in death of the host. The surviving animals demonstrated a high degree of immunity to challenge with live, virulent organisms. Two injections with 15 mug of the antigen produced a high degree of immunity in mice without the development of any signs of toxicity. The gross chemical composition and toxicity of the antigen were similar to those reported for endotoxins obtained by the Boivin or Westphal procedure. Although strong serological cross-reactions were obtained in Ouchterlony plates between the 105,000 x g antigens from the bison strain and an avian strain with antisera to these strains, these antisera agglutinated only the bacterial cells of the homologous strain. The active immunity obtained in mice by the injection of the 105,000 x g antigens of each strain was specific and could be correlated with the agglutination tests.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Rebers PA,Heddleston KL,Rhoades KR

doi

10.1128/JB.93.1.7-14.1967

subject

Has Abstract

pub_date

1967-01-01 00:00:00

pages

7-14

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

93

pub_type

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