Abstract:
:The activation properties of the form I and form II ribulose 1,5-bisphosphate carboxylases from Rhodopseudomonas sphaeroides were examined. Both enzymes have a requirement of Mg2+ for optimal activity. Mn2+, Ni2+, and Co2+ can also support activity of the form I enzyme, whereas only Mn2+ can substitute for Mg2+ with the form II enzyme. The effect of different preincubations on the carboxylase reaction was also examined. Both enzymes exhibited a lag when preincubated with other than Mg2+ and CO2 before assay, but the lag was much more pronounced and the rate of the reaction was slower with the form I enzyme under these conditions. Activation of the form I carboxylase By Mg2+ and CO2 occurred more rapidly than that of the form II enzyme. The results obtained with the two distinct forms of carboxylase from R. sphaeroides, as well as studies with the spinach and Rhodospirillum rubrum enzymes, thus indicate that the presence of the small subunit affects the rate of activation by Mg2+ and CO2 as well as the rate of reactivation of ribulose bisphosphate-inactivated enzyme.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Gibson JL,Tabita FRdoi
10.1128/JB.140.3.1023-1027.1979subject
Has Abstractpub_date
1979-12-01 00:00:00pages
1023-7issue
3eissn
0021-9193issn
1098-5530journal_volume
140pub_type
杂志文章abstract::Razin, S. (University of Connecticut, Storrs), M. E. Tourtellotte, R. N. McElhaney, and J. D. Pollack. Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells. J. Bacteriol. 91:609-616. 1966.-Lipid composition of Mycoplasma laidlawii membranes could be significantly changed by var...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.609-616.1966
更新日期:1966-02-01 00:00:00
abstract::In bacteria, normal mutation frequencies are mostly around 10(-10) per base pair. However, there exists natural isolates, called "mutators," that exhibit permanent mutation occurrences up to 1,000-fold greater than usual. As mutations play essential roles, particularly in the evolution of antibiotic resistance, bacter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00498-12
更新日期:2012-07-01 00:00:00
abstract::Commonly used procedures effected the extraction of only 10% of the lipoteichoic acid of stationary-phase cells of Staphylococcus aureus and Streptococcus faecium, unless the cells were first disrupted. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.1.399-402.1982
更新日期:1982-01-01 00:00:00
abstract::The Saccharomyces cerevisiae mating hormone a-factor has been difficult to isolate reproducibly in sufficient yields by methods using ion-exchange chromatography, probably because of its pronounced hydrophobicity. In this work, a hydrophobic adsorbent (Amberlite XAD-2), in an insoluble bead from, was used to isolate l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.3.1153-1161.1982
更新日期:1982-09-01 00:00:00
abstract::Spectinomycin dependence in Bacillus subtilis involves two mutations, one conferring drug resistance and the other producing a requirement for spectinomycin for growth. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.3.1452-1455.1979
更新日期:1979-03-01 00:00:00
abstract::The gene (hmgA) for 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (EC 1.1.1.34) from the thermophilic archaeon Sulfolobus solfataricus P2 was cloned and sequenced. S. solfataricus HMG-CoA reductase exhibited a high degree of sequence identity (47%) to the HMG-CoA reductase of the halophilic archaeon Halofe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.11.3632-3638.1997
更新日期:1997-06-01 00:00:00
abstract::Streptococcus salivarius, a gram-positive bacterium found in the human oral cavity, expresses flexible peritrichous fimbriae. In this paper, we report purification and partial characterization of S. salivarius fimbriae. Fimbriae were extracted by shearing the cell surface of hyperfimbriated mutant A37 (a spontaneous m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.9.2724-2732.2001
更新日期:2001-05-01 00:00:00
abstract::The DNA sequence of the central regulatory locus vir of Bordetella pertussis predicts that three gene products, BvgA, BvgB, and BvgC, are encoded. Features of the predicted primary structures of these proteins and their homology to other two-component systems suggest that BvgA is located in the cytoplasm, BvgB is loca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.14.4288-4296.1991
更新日期:1991-07-01 00:00:00
abstract::A genetic element integrating site specifically into a prokaryotic gene usually carries a copy of the 3' portion of that gene that restores the active gene even as the original is disrupted. A cryptic element in Mesorhizobium loti instead carries a copy of the 5' end of the tRNA gene into which it integrated. This has...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.3.859-860.2002
更新日期:2002-02-01 00:00:00
abstract::Acidic hot springs are colonized by a diversity of hyperthermophilic organisms requiring extremes of temperature and pH for growth. To clarify how carbohydrates are consumed in such locations, the structural gene (malA) encoding the major soluble alpha-glucosidase (maltase) and flanking sequences from Sulfolobus solfa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.5.1287-1295.1998
更新日期:1998-03-01 00:00:00
abstract::Tetracycline resistance of three Bacteroides fragilis strains was shown to be inducible by subinhibitory concentrations of tetracycline. Tetracycline resistance markers could be transferred to another B. fragilis strain by filter mating. The transferability was inducible by subinhibitory concentrations of tetracycline...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.1.141-147.1982
更新日期:1982-04-01 00:00:00
abstract::The Gram-positive bacterium Streptococcus pyogenes (also called group A Streptococcus [GAS]), is found strictly in humans and is capable of causing a wide variety of infections. Here we demonstrate that serine catabolism in GAS is controlled by the transcriptional regulator Spy49_0126c. We have designated this regulat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01516-10
更新日期:2011-04-01 00:00:00
abstract::The acetohydroxy acid synthase III isozyme, which catalyzes the first common step in the biosynthesis of isoleucine, leucine, and valine in Escherichia coli K-12, is composed of two subunits, the ilvI and ilvH gene products. A missense mutation in ilvH (ilvH612), which reduced the sensitivity of the enzyme to the end ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.11.5197-5199.1988
更新日期:1988-11-01 00:00:00
abstract::Through hybridization of available probes, we have added nine genes to the macrorestriction map of the Haloferax mediterranei chromosome and five genes to the contig map of Haloferax volcanii. Additionally, we hybridized 17 of the mapped cosmid clones from H. volcanii to the H. mediterranei genome. The resulting 35-po...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.5.1405-1408.1995
更新日期:1995-03-01 00:00:00
abstract::Prokaryotic organisms occupy the most diverse set of environments and conditions on our planet. Their ability to sense and respond to a broad range of external cues remain key research areas in modern microbiology, central to behaviors that underlie beneficial and pathogenic interactions of bacteria with multicellular...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00439-19
更新日期:2019-09-20 00:00:00
abstract::Phosphoenolpyruvate (PEP) carboxykinase was identified to be the only C3-carboxylating enzyme in Alcaligenes eutrophus. The enzyme requires GDP or inosine diphosphate (GTP or inosine triphosphate) for activity. Pyruvate- and other PEP-dependent CO2-fixing enzyme activities were not detected, regardless of whether the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.1.167-172.1984
更新日期:1984-07-01 00:00:00
abstract::To study the role of the cis-acting element(s) in controlling the expression of the cytochrome P450(BM-1) gene and its upstream regulatory gene, bm1P1, in Bacillus megaterium, various deletion derivatives were constructed. A 53-bp inverted repeat located midway between the P450(BM-1) gene and bm1P1 gene was found in v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.1.280-283.1997
更新日期:1997-01-01 00:00:00
abstract::Genes from Bacillus subtilis predicted to encode a phosphate-specific transport (Pst) system were shown by mutation to affect high-affinity Pi uptake but not arsenate resistance or phosphate (Pho) regulation. The transcription start of the promoter upstream of the pstS gene was defined by primer extension. The promote...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.8.2534-2539.1997
更新日期:1997-04-01 00:00:00
abstract::In contrast to canonical phage endolysins, which require holin-mediated disruption of the membrane to gain access to attack the cell wall, signal anchor release (SAR) endolysins are secreted by the host sec system, where they accumulate in an inactive form tethered to the membrane by their N-terminal SAR domains. SAR ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00674-10
更新日期:2010-11-01 00:00:00
abstract::The fim genetic switch in the chromosome of Escherichia coli K-12 is an invertible DNA element that harbors the promoter for transcription of the downstream fim structural genes and a transcription terminator that acts on the upstream fimE regulatory gene. Switches oriented appropriately for structural gene transcript...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.24.8256-8266.2005
更新日期:2005-12-01 00:00:00
abstract::Western blot (immunoblot) analysis of cell extracts from induced bacteriophage lambda lysogens probed with S-protein-specific antibody (raised against an S--beta-galactosidase fusion protein) demonstrated that the bacteriophage lambda S protein begins to appear 10 min after phage induction and is localized to the inne...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.912-921.1990
更新日期:1990-02-01 00:00:00
abstract::Haight, Rodger D. (Oregon State University, Corvallis), and Richard Y. Morita. Thermally induced leakage from Vibrio marinus, an obligately psychrophilic bacterium. J. Bacteriol. 92:1388-1393. 1966.-Leakage of various cellular components into the surrounding menstruum occurred when Vibrio marinus was subjected to temp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.5.1388-1393.1966
更新日期:1966-11-01 00:00:00
abstract::The two highly homologous cbb operons of Alcaligenes eutrophus H16 that are located on the chromosome and on megaplasmid pHG1 contain genes encoding several enzymes of the Calvin carbon reduction cycle. Sequence analysis of a region from the promoter-distal part revealed two open reading frames, designated cbbT and cb...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.22.7329-7340.1993
更新日期:1993-11-01 00:00:00
abstract::Escherichia coli lactose permease mediates the proton-driven translocation of galactosides across the cytoplasmic membrane. To define regions important for membrane insertion as well as for biological function, we constructed plasmids encoding different portions of the lactose carrier. Among several lacY deletions, tw...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.5374-5381.1990
更新日期:1990-09-01 00:00:00
abstract::A combined physical and genetic map of the Serpulina hyodysenteriae B78T genome was constructed by using pulsed-field gel electrophoresis and DNA blot hybridizations. The S. hyodysenteriae genome is a single circular chromosome about 3.2 Mb in size. The physical map of the chromosome was constructed with the restricti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.4.1087-1092.1994
更新日期:1994-02-01 00:00:00
abstract::In Staphylococcus aureus, the transport of dicarboxylates is mediated in part by the Na+-linked carrier protein SdcS. This transporter is a member of the divalent-anion/Na+ symporter (DASS) family, a group that includes the mammalian Na+/dicarboxylate cotransporters NaDC1 and NaDC3. In earlier work, we cloned and expr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01452-06
更新日期:2007-02-01 00:00:00
abstract::SecB is a cytosolic protein required for rapid and efficient export of particular periplasmic and outer membrane proteins in Escherichia coli. SecB promotes export by stabilizing newly synthesized precursor proteins in a nonnative conformation and by targeting the precursors to the inner membrane. Biochemical studies ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.10.3010-3017.1999
更新日期:1999-05-01 00:00:00
abstract::Bacillus anthracis Sterne cured of the pXO1 plasmid had enhanced secreted protease activity during the postexponential phase but no change in hemolytic or lecithinase activities. A zymogen profile revealed at least six proteases, including serine, metal, and perhaps cysteine types. There were similar amounts of protea...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.9.3133-3138.2005
更新日期:2005-05-01 00:00:00
abstract::Previously we have shown that flagella and the O-specific polysaccharide of lipopolysaccharide play a role in colonization of the potato root by plant growth-promoting Pseudomonas strains WCS374 and WCS358. In this paper, we describe a novel cell surface-exposed structure in Pseudomonas putida WCS358 examined with a s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.7.1955-1961.1996
更新日期:1996-04-01 00:00:00
abstract::The levels of transfer ribonucleic acids (tRNAs) specific for 14 amino acids were almost identical in dormant spores and in spores germinated from 6 to 75 min. Germinated spore tRNAs specific for all amino acids tested were between 63 and 93% charged, and there was no significant change in this value from 6 to 75 min ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.3.1067-1074.1974
更新日期:1974-06-01 00:00:00