USE OF S-METHYLCYSTINE AND CYSTATHIONINE BY METHIONINELESS NEUROSPORA MUTANTS.

Abstract:

:Wiebers, Joyce L. (Purdue University, West Lafayette, Ind.), and Harold R. Garner. Use of S-methylcysteine and cystathionine by methionineless Neurospora mutants. J. Bacteriol. 88:1798-1804. 1964.-Radioactive methionine was found in hydrolysates of various strains of Neurospora crassa when either S-methylcysteine (SMC)-C(14)H(3) or SMC-S(35) is the sole addition to minimal medium. Isotope product-precursor specific activity ratios are very similar for the two sources of label. Wild-type and methionineless mutants use sulfur from SMC in the biosynthesis of methionine, but not of cysteine, when grown in regular medium. With a medium nearly free from sulfate, SMC served as a source of sulfur for both cysteine and methionine. Suppressed methionineless mutants incorporated sulfur from SMC into cellular cysteine even in the presence of normal amounts of sulfate. SMC as a possible metabolic precursor of methionine was compared to cystathionine in an experiment with wild-type Neurospora. The four sources of label used were: SMC-C(14)H(3), SMC-S(35), cystathionine-U-C(14), and cystathionine-S(35). In each flask, the organism was offered one of the labeled compounds plus an equivalent amount of the other compound without label. The amount of each compound was sufficient for either to supply its contribution to all of the cellular methionine, if it were successful in competing with endogenous sources. To avoid adaptive breakdown of substrates, the compounds were added continuously at a rate consistent with the amount of growth present. The ratio of specific activity of cellular methionine to precursor was determined for each labeled compound. The results show that SMC sulfur and methyl carbon are used equally well. Cystathionine carbon and sulfur appear to be equally utilized also. A preference for cystathionine is indicated.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

WIEBERS JL,GARNER HR

doi

10.1128/JB.88.6.1798-1804.1964

keywords:

subject

Has Abstract

pub_date

1964-12-01 00:00:00

pages

1798-804

eissn

0021-9193

issn

1098-5530

journal_volume

88

pub_type

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