Participation of lipopolysaccharide genes in the determination of the enterobacterial common antigen: analysis of R mutants of Salmonella minnesota.

Abstract:

:A series of R (rough) Salmonella minnesota mutants with rfb, rfe, and rfa mutations leading to various defects in the biosynthesis of cell wall lipopolysaccharide was analyzed as to their enterobacterial common antigen (CA) content. All mutants that had functional rfe genes were CA(+) as is the wild-type parent. This includes mutants with the most defective lipopolysaccharide core types, demonstrating that core structures are not a necessary part of CA. All rfe(-) mutants (complete lipopolysaccharide core, defective synthesis of O side chains) were defective in the synthesis of CA. A smooth strain was accidentally found to be CA(-); the mutation responsible for this defect was also located, like rfe, very close to ilv.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Mäkelä PH,Mayer H,Whang HY,Neter E

doi

10.1128/JB.119.3.760-764.1974

subject

Has Abstract

pub_date

1974-09-01 00:00:00

pages

760-4

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

119

pub_type

杂志文章
  • Sequence of xynC and properties of XynC, a major component of the Clostridium thermocellum cellulosome.

    abstract::The nucleotide sequence of the Clostridium thermocellum F1 xynC gene, which encodes the xylanase XynC, consists of 1,857 bp and encodes a protein of 619 amino acids with a molecular weight of 69,517. XynC contains a typical N-terminal signal peptide of 32 amino acid residues, followed by a 165-amino-acid sequence whic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.13.4246-4253.1997

    authors: Hayashi H,Takagi KI,Fukumura M,Kimura T,Karita S,Sakka K,Ohmiya K

    更新日期:1997-07-01 00:00:00

  • Visualization of ribosomal ribonucleic acid synthesis in a ribonuclease III-Deficient strain of Escherichia coli.

    abstract::Transmission electron microscopy was used to examine active ribosomal ribonucleic acid (rRNA) genes in two strains of Escherichia coli: N2077, deficient in the enzyme responsible for proper cleavage of the 16S sequence from the elongating nascent rRNA transcript; and N2076, functional in ribonuclease (RNase) III activ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.2.718-722.1977

    authors: Hofmann S,Miller OL Jr

    更新日期:1977-11-01 00:00:00

  • CsgA, an extracellular protein essential for Myxococcus xanthus development.

    abstract::CsgA mutants of Myxococcus xanthus appear to be defective in producing an extracellular molecule essential for the developmental behaviors of this bacterium. The csgA gene encodes a 17.7-kilodalton polypeptide whose function and cellular location were investigated with immunological probes. Large quantities of the Csg...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.9.5299-5306.1990

    authors: Shimkets LJ,Rafiee H

    更新日期:1990-09-01 00:00:00

  • Regulation of nitrogenase gene expression by transcript stability in the cyanobacterium Anabaena variabilis.

    abstract::The nitrogenase gene cluster in cyanobacteria has been thought to comprise multiple operons; however, in Anabaena variabilis, the promoter for the first gene in the cluster, nifB1, appeared to be the primary promoter for the entire nif cluster. The structural genes nifHDK1 were the most abundant transcripts; however, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02045-14

    authors: Pratte BS,Thiel T

    更新日期:2014-10-01 00:00:00

  • Nuclear deoxyribonucleic acid-dependent ribonucleic acid polymerases from Saccharomyces cerevisiae.

    abstract::Two deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerases (I, II) have been solubilized from isolated Saccharomyces cerevisiae nuclei. The enzymes can be separated by chromatography on O-diethylaminoethyl Sephadex. Both enzymes are active with high-molecular-weight nuclear yeast DNA, although RNA po...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.1.1-6.1973

    authors: Sebastian J,Bhargava MM,Halvorson HO

    更新日期:1973-04-01 00:00:00

  • Thermoregulation of virB transcription in Shigella flexneri by sensing of changes in local DNA superhelicity.

    abstract::Transcription of the virB gene, a transcriptional regulator of invasion genes on the large plasmid of Shigella flexneri, is strictly regulated by growth temperature; when bacteria are grown at 37 degrees C, virB transcription is highly activated, while at 30 degrees C the level of virB transcription decreases to less ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.4.1094-1097.1995

    authors: Tobe T,Yoshikawa M,Sasakawa C

    更新日期:1995-02-01 00:00:00

  • Recruitment of the ParG segregation protein to different affinity DNA sites.

    abstract::The segrosome is the nucleoprotein complex that mediates accurate plasmid segregation. In addition to its multifunctional role in segrosome assembly, the ParG protein of multiresistance plasmid TP228 is a transcriptional repressor of the parFG partition genes. ParG is a homodimeric DNA binding protein, with C-terminal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01630-08

    authors: Zampini M,Derome A,Bailey SE,Barillà D,Hayes F

    更新日期:2009-06-01 00:00:00

  • Identification of a protein of 15,000 daltons related to isoleucine-valine biosynthesis in Escherichia coli K-12.

    abstract::The effect of the ilvG671, ilvG468, and ilvG603 mutations (phenotype, IlvG+ Valr; formerly ilvO) upon proteins synthesized was determined by infection of irradiated Escherichia coli K-12 cells, using specifically constructed derivatives of lambda dilv phage. These ilvG alleles are similar to the previously studied ilv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.1.127-134.1982

    authors: Gray JE,Wallen JW,Calhoun DH

    更新日期:1982-07-01 00:00:00

  • Identification and DNA sequence of the mobilization region of the 5-nitroimidazole resistance plasmid pIP421 from Bacteroides fragilis.

    abstract::The nucleotide sequence of the DNA mobilization region of the 5-nitroimidazole resistance plasmid pIP421, from strain BF-F239 of Bacteroides fragilis, was determined. It contains a putative origin of transfer (oriT) including three sets of inverted repeats and two sequences reminiscent of specific integration host fac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.12.4071-4074.1997

    authors: Trinh S,Reysset G

    更新日期:1997-06-01 00:00:00

  • Characteristics of cold-sensitive mutants of Escherichia coli K-12 defective in deoxyribonucleic acid replication.

    abstract::Four cold-sensitive mutants of Escherichia coli have been isolated which show a reduced ability to synthesize deoxyribonucleic acid at low temperature. The mutants also have a reduced ability to incorporate nucleoside triphosphates into deoxyribonucleic acid at low temperature in cell preparations made permeable with ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.1.99-107.1975

    authors: Wehr CT,Waskell L,Glaser DA

    更新日期:1975-01-01 00:00:00

  • Homology among Escherichia coli K1 and K92 polysialytransferases.

    abstract::The neuS-encoded polysialytransferase (polyST) in Escherichia coli K1 catalyzes synthesis of polysialic acid homopolymers composed of unbranched sialyl alpha 2,8 linkages. Subcloning and complementation experiments showed that the K1 neuS was functionally interchangeable with the neuS from E. coli K92 (S. M. Steenberg...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.15.5127-5131.1992

    authors: Vimr ER,Bergstrom R,Steenbergen SM,Boulnois G,Roberts I

    更新日期:1992-08-01 00:00:00

  • Levels of enzymes involved in the synthesis of acetate from CO2 in Clostridium thermoautotrophicum.

    abstract::The acetogenic bacterium Clostridium thermoautotrophicum, grown on methanol, glucose, or CO2-H2, contained high levels of corrinoids, formate dehydrogenase, tetrahydrofolate enzymes, carbon monoxide dehydrogenase, and hydrogenase. Cell-free extracts catalyzed pyruvate-dependent formation of acetate from methyltetrahyd...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.1.507-509.1982

    authors: Clark JE,Ragsdale SW,Ljungdahl LG,Wiegel J

    更新日期:1982-07-01 00:00:00

  • Repression of 3-deoxy-D-arabinoheptulosonic acid-7-phosphate synthetase (trp) and enzymes of the tryptophan pathway in Escherichia coli K-12.

    abstract::Mutant strains of Escherichia coli K-12 have been isolated in which the synthesis of 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase (trp) is partially constitutive. The mutation causing derepression is closely linked to aroH [the structural gene for DAHP synthetase (trp)] and occurs in a locus design...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.107.2.406-414.1971

    authors: Camakaris J,Pittard J

    更新日期:1971-08-01 00:00:00

  • Enhanced synthesis of internalin A in aro mutants of Listeria monocytogenes indicates posttranscriptional control of the inlAB mRNA.

    abstract::Listeria monocytogenes mutants with deletions in aroA, aroB, or aroE exhibited strong posttranscriptional upregulation of internalin A (InlA) and InlB synthesis, which resulted in a more-than-10-fold increase in InlA-mediated internalization by epithelial Caco-2 cells and a 4-fold increase in InlB-mediated internaliza...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.8.2836-2845.2005

    authors: Stritzker J,Schoen C,Goebel W

    更新日期:2005-04-01 00:00:00

  • Bacillus subtilis mutant allele sup-3 causes lysine insertion at ochre codons: use of sup-3 in studies of translational attenuation.

    abstract::The mutation sup-3 in Bacillus subtilis suppresses ochre (TAA) mutations at each of three codons in the 5' end of the cat-86 coding sequence. The suppressor is shown to insert lysine at ochre codons. The efficiency of suppression by sup-3 is about 15%, as determined by changing a cat-86 Lys codon (codon 12) to an ochr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.10.5322-5324.1989

    authors: Mulbry WW,Ambulos NP Jr,Lovett PS

    更新日期:1989-10-01 00:00:00

  • The narJ gene product is required for biogenesis of respiratory nitrate reductase in Escherichia coli.

    abstract::Respiratory nitrate reductase purified from the cell membrane of Escherichia coli is composed of three subunits, alpha, beta, and gamma, which are encoded, respectively, by the narG, narH, and narI genes of the narGHJI operon. The product of the narJ gene was deduced previously to be a highly charged, acidic protein w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.3.867-872.1992

    authors: Dubourdieu M,DeMoss JA

    更新日期:1992-02-01 00:00:00

  • Role of adaptor TrfA and ClpPC in controlling levels of SsrA-tagged proteins and antitoxins in Staphylococcus aureus.

    abstract::Staphylococcus aureus responds to changing extracellular environments in part by adjusting its proteome through alterations of transcriptional priorities and selective degradation of the preexisting pool of proteins. In Bacillus subtilis, the proteolytic adaptor protein MecA has been shown to play a role in assisting ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02222-14

    authors: Donegan NP,Marvin JS,Cheung AL

    更新日期:2014-12-01 00:00:00

  • Effects of oxygen and heme on the development of a microbial respiratory system.

    abstract::The effect of adding hemin to anaerobically grown cells of a strain of Staphylococcus epidermidis, which was heme-deficient due to anaerobic growth, has been examined. Cells grown anaerobically in media containing hemin exhibited a marked increase in several oxidative activities as compared with cells grown anaerobica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.1.278-285.1967

    authors: Jacobs NJ,Maclosky ER,Conti SF

    更新日期:1967-01-01 00:00:00

  • Guanine uptake and metabolism in Neurospora crassa.

    abstract::Guanine is transported into germinated conidia of Neurospora crassa by the general purine base transport system. Guanine uptake is inhibited by adenine and hypoxanthine but not xanthine. Guanine phosphoribosyltransferase (GPRTase) activity was demonstrated in cell extracts of wild-type germinated conidia. The Km for g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.3.941-947.1982

    authors: Magill CW,Sabina RL,Garber TL,Magill JM

    更新日期:1982-03-01 00:00:00

  • Coordination of Synthesis and Assembly of a Modular Membrane-Associated [NiFe]-Hydrogenase Is Determined by Cleavage of the C-Terminal Peptide.

    abstract:UNLABELLED:During biosynthesis of [NiFe]-hydrogenase 2 (Hyd-2) of Escherichia coli, a 15-amino-acid C-terminal peptide is cleaved from the catalytic large subunit precursor, pro-HybC. This peptide is removed only after NiFe(CN)2CO cofactor insertion by the Hyp accessory protein machinery has been completed, suggesting ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00437-15

    authors: Thomas C,Muhr E,Sawers RG

    更新日期:2015-09-01 00:00:00

  • Occurrence of homologs of the Escherichia coli lytB gene in gram-negative bacterial species.

    abstract::The Escherichia coli LytB protein regulates the activity of guanosine 3',5'-bispyrophosphate synthetase I (RelA). A Southern blot analysis of chromosomal DNA with the E. coli lytB gene as a probe revealed the presence of lytB homologs in all of the gram-negative bacterial species examined but not in gram-positive spec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.7.1959-1961.1998

    authors: Potter S,Yang X,Boulanger MJ,Ishiguro EE

    更新日期:1998-04-01 00:00:00

  • Taxonomy of psychrophilic strains of Bacillus.

    abstract::The morphological and physiological characteristics of 20 isolates of psychrophilic Bacillus were compared with 29 strains representing nine species of mesophilic Bacillus and 2 strains of Sporosarcina ureae to determine the taxonomic position of the psychrophiles. The psychrophiles formed four distinct groups which w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.4.889-895.1967

    authors: Larkin JM,Stokes JL

    更新日期:1967-10-01 00:00:00

  • Minimum domain of the Shiga toxin A subunit required for enzymatic activity.

    abstract::The minimum sequence of the enzymatic (A) subunit of Shiga toxin (STX) required for activity was investigated by introducing N-terminal and C-terminal deletions in the molecule. Enzymatic activity was assessed by using an in vitro translation system. A 253-amino-acid STX A polypeptide, which is recognized as the enzym...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.16.4970-4978.1993

    authors: Haddad JE,al-Jaufy AY,Jackson MP

    更新日期:1993-08-01 00:00:00

  • Regulation of orotidylic acid pyrophosphorylase in Saccharomyces cerevisiae.

    abstract::Yeast mutants deficient in orotidine-5'-phosphate (OMP) pyrophosphorylase activity have been obtained. The gene encoding this enzyme is unlinked to the four pyrimidine genes previously described. The specific activity of OMP pyrophosphorylase remains constant under all of the physiological conditions used to repress, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.1.196-202.1972

    authors: Jund R,Lacroute F

    更新日期:1972-01-01 00:00:00

  • In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae.

    abstract::In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.6.2876-2880.1987

    authors: Wong PK,Popham D,Keener J,Kustu S

    更新日期:1987-06-01 00:00:00

  • Unbalanced growth death due to depletion of Mn2+ in Brevibacterium ammoniagenes.

    abstract::In the microbial conversion of added hypoxanthine to 5'-inosinic acid, Mn(2+) concentration in the growth medium is known to have a profound effect both on the yield of 5'-inosinic acid and the morphology of cells of Brevibacterium ammoniagenes. To elucidate the mechanism in which Mn(2+) was concerned with cell morpho...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.5.1760-1767.1968

    authors: Oka T,Udagawa K,Kinoshita S

    更新日期:1968-11-01 00:00:00

  • Function of a mycobacterial major facilitator superfamily pump requires a membrane-associated lipoprotein.

    abstract::The lprG-Rv1410c operon is critical for the survival of Mycobacterium tuberculosis during infection, but very little is known about the functions of its proteins. LprG is a lipoprotein, and Rv1410c encodes the major facilitator superfamily small molecule transporter P55. P55 likely exports small molecules outside of t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01046-07

    authors: Farrow MF,Rubin EJ

    更新日期:2008-03-01 00:00:00

  • Dynamics of two Phosphorelays controlling cell cycle progression in Caulobacter crescentus.

    abstract::In Caulobacter crescentus, progression through the cell cycle is governed by the periodic activation and inactivation of the master regulator CtrA. Two phosphorelays, each initiating with the histidine kinase CckA, promote CtrA activation by driving its phosphorylation and by inactivating its proteolysis. Here, we exa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00992-09

    authors: Chen YE,Tsokos CG,Biondi EG,Perchuk BS,Laub MT

    更新日期:2009-12-01 00:00:00

  • Recombination in the ompA gene but not the omcB gene of Chlamydia contributes to serovar-specific differences in tissue tropism, immune surveillance, and persistence of the organism.

    abstract::Sequences of the major outer membrane protein (MOMP) gene (ompA) and the outer membrane complex B protein gene (omcB) from Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydia psittaci were analyzed for evidence of intragenic recombination and for linkage equilibrium. The Sawyer runs test, compatibility matrices...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.20.5997-6008.2001

    authors: Millman KL,Tavaré S,Dean D

    更新日期:2001-10-01 00:00:00

  • Preparation of metabolically active Staphylococcus aureus protoplasts by use of the Aeromonas hydrophila lytic enzyme.

    abstract::Stable, metabolically active protoplasts of Staphylococcus aureus have been prepared by the use of a staphylolytic enzyme produced by Aeromonas hydrophila. Respiratory and glycolytic rates and synthesis of nucleic acids, protein, and lipid in these protoplasts, stabilized variously in 1.1 M sucrose, 0.37 M sodium succ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.3.746-751.1973

    authors: Coles NW,Gross R

    更新日期:1973-09-01 00:00:00