Amikacin resistance mediated by multiresistance transposon Tn2424.

Abstract:

:Tn2424, a multiresistance transposon 25 kilobases long, was isolated from IncFII plasmid NR79. Tn2424 transposed resistance to sulfonamides, streptomycin and spectinomycin, mercuric chloride, chloramphenicol, and amikacin with a frequency of 6 X 10(-5). Resistance to amikacin was mediated by a 6'-N-acetyltransferase, which conferred higher levels of resistance in Pseudomonas aeruginosa than in Escherichia coli. A restriction analysis and cloning experiments resulted in a physical and functional map of Tn2424. Comparison by a heteroduplex technique revealed that Tn2424 includes the total sequence of Tn21 and two additional DNA fragments that are 1.8 and 4 kilobases long.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Meyer JF,Nies BA,Wiedemann B

doi

10.1128/JB.155.2.755-760.1983

subject

Has Abstract

pub_date

1983-08-01 00:00:00

pages

755-60

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

155

pub_type

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