Abstract:
:Nitrogenase activities were determined from maximum acetylene reduction rates for mutant strains of Azotobacter vinelandii which are unable to fix N2 in the presence of molybdenum (Nif-) but undergo phenotypic reversal to Nif+ under conditions of Mo deficiency. The system responsible for N2 fixation under these conditions is thought to be an alternative N2 fixation system (Bishop et al., Proc. Natl. Acad. Sci. U.S.A. 77:7342-7346, 1980). Phenotypic reversal of Nif- strains to Nif+ strains was also observed in N-free medium without Mo but with either V or Re. Two protein patterns were found on two-dimensional gels of proteins from the extracts of wild-type cells cultured in N-free medium without Mo and with or without V or Re. The expression of each protein pattern in the wild-type strain of A. vinelandii seemed to depend upon the physiological state of the N2-fixing culture. Electron paramagnetic resonance experiments were conducted on whole cells of A. vinelandii grown under conditions of Mo deprivation in the absence of fixed N. No g = 3.65 signal (an electron paramagnetic resonance signal characteristic of the Mo-containing component of nitrogenase) was detectable in these cells, regardless of whether V or Re was present during growth of these cells, These results are discussed from the perspective that the well-known effect of V on N2 fixation by A. vinelandii may involve an alternative N2 fixation system.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Bishop PE,Jarlenski DM,Hetherington DRdoi
10.1128/JB.150.3.1244-1251.1982subject
Has Abstractpub_date
1982-06-01 00:00:00pages
1244-51issue
3eissn
0021-9193issn
1098-5530journal_volume
150pub_type
杂志文章abstract::The Lyme disease spirochete Borrelia burgdorferi is unique among bacteria in its large number of lipoproteins that are encoded by a small, exceptionally fragmented, and predominantly linear genome. Peripherally anchored in either the inner or outer membrane and facing either the periplasm or the external environment, ...
journal_title:Journal of bacteriology
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更新日期:1995-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1969-07-01 00:00:00
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更新日期:2000-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章,收录出版
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更新日期:2004-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.3.992-998.1986
更新日期:1986-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.148.2.728-729.1981
更新日期:1981-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.512-521.1969
更新日期:1969-10-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2012-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.143.3.1498-1500.1980
更新日期:1980-09-01 00:00:00
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更新日期:2010-01-01 00:00:00
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更新日期:1965-07-01 00:00:00
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更新日期:2005-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1964-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-12-01 00:00:00
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更新日期:1970-09-01 00:00:00
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更新日期:1999-10-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.161.1.249-257.1985
更新日期:1985-01-01 00:00:00
abstract::Martin, Alice J. (Nassau County Department of Health, Hempstead, N.Y.), and Margaret O'Brien. Detection of enteropathogenic Escherichia coli in fecal cultures by use of a modified fluorescent-antibody technique. J. Bacteriol. 89:570-573. 1965.-The application of the fluorescent-antibody (FA) technique to cultures of f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1965-03-01 00:00:00
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更新日期:2010-03-01 00:00:00
abstract::The roles of three TATA binding protein (TBP) homologs (TBP1, TBP2, and TBP3) in the archaeon Methanosarcina acetivorans were investigated by using genetic and molecular approaches. Although tbp2 and tbp3 deletion mutants were readily obtained, a tbp1 mutant was not obtained, and the growth of a conditional tbp1 expre...
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更新日期:2010-03-01 00:00:00