Gluconate metabolism in Escherichia coli.

Abstract:

:On the basis of information available in the literature, gluconate dissimilation in Escherichia coli is thought to occur via the hexose monophosphate pathway. Evidence is presented in this study that gluconate is catabolized in this organism via an inducible Entner-Doudoroff pathway. This evidence is based on chromatographic examination of end products produced from (14)C-labeled gluconate or glucose, distribution of (14)C in the carbon atoms of pyruvate formed from specifically labeled (14)C-glucose and (14)C-gluconate, and the ability of cell-free extracts to produce pyruvate from 6-phosphogluconate. Degradation of gluconate by an Entner-Doudoroff pathway occurred simultaneously with a glycolytic cleavage of glucose. A relationship between gluconate-induced, Entner-Doudoroff pathway activity and catabolism of glucose in Escherichia coli and other bacterial species is discussed.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Eisenberg RC,Dobrogosz WJ

doi

10.1128/JB.93.3.941-949.1967

subject

Has Abstract

pub_date

1967-03-01 00:00:00

pages

941-9

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

93

pub_type

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