Abstract:
:On the basis of information available in the literature, gluconate dissimilation in Escherichia coli is thought to occur via the hexose monophosphate pathway. Evidence is presented in this study that gluconate is catabolized in this organism via an inducible Entner-Doudoroff pathway. This evidence is based on chromatographic examination of end products produced from (14)C-labeled gluconate or glucose, distribution of (14)C in the carbon atoms of pyruvate formed from specifically labeled (14)C-glucose and (14)C-gluconate, and the ability of cell-free extracts to produce pyruvate from 6-phosphogluconate. Degradation of gluconate by an Entner-Doudoroff pathway occurred simultaneously with a glycolytic cleavage of glucose. A relationship between gluconate-induced, Entner-Doudoroff pathway activity and catabolism of glucose in Escherichia coli and other bacterial species is discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Eisenberg RC,Dobrogosz WJdoi
10.1128/JB.93.3.941-949.1967subject
Has Abstractpub_date
1967-03-01 00:00:00pages
941-9issue
3eissn
0021-9193issn
1098-5530journal_volume
93pub_type
杂志文章abstract::Pseudomonas syringae injects numerous bacterial proteins into host plant cells through a type 3 secretion system (T3SS). One of the first such bacterial effectors discovered, HopA1, is a protein that has unknown functions in the host cell but possesses close homologs that trigger the plant hypersensitive response in r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01621-12
更新日期:2013-02-01 00:00:00
abstract::The symbiotic interaction between Rhizobium etli and Phaseolus vulgaris, the common bean plant, ultimately results in the formation of nitrogen-fixing nodules. Many aspects of the intermediate and late stages of this interaction are still poorly understood. The R. etli relA gene was identified through a genome-wide sc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.15.5460-5469.2005
更新日期:2005-08-01 00:00:00
abstract::Here we show that the type III secretion gatekeeper protein SepL resembles an aberrant effector protein in binding to a class 1 type III secretion chaperone (Orf12, here renamed CesL). We also show that short N-terminal fragments (≤70 amino acids) from SepL are capable of targeting fusion proteins for secretion and tr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00760-10
更新日期:2010-11-01 00:00:00
abstract::Azotobacter vinelandii was grown diazotrophically in chemostat cultures limited by sucrose, citrate, or acetate. Specific activities of cellular oxygen consumption (qO2) and nitrogenase (acetylene reduction) were measured in situ at different dilution rates (D, representing the specific growth rate mu at steady state)...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.11.5325-5329.1988
更新日期:1988-11-01 00:00:00
abstract::Here we present the genome sequence of Rhizobium grahamii CCGE502. R. grahamii groups with other newly described broad-host-range species, which are not very efficient Phaseolus vulgaris symbionts, with a wide geographic distribution and which constitutes a novel Rhizobium clade. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01785-12
更新日期:2012-12-01 00:00:00
abstract::A Bacillus subtilis sigM null mutant, lacking the extracytoplasmic function sigma(M) protein, was sensitive to paraquat (PQ), a superoxide-generating reagent, but not to the redox stress-inducing compounds hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide, or diamide. Surprisingly, a sigM mutant was only ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.9.2948-2956.2005
更新日期:2005-05-01 00:00:00
abstract::In previous work, we cloned a Candida albicans cDNA for the glycolytic enzyme enolase and found a single, abundant enolase transcript on Northern (RNA) blots and a single protein on immunoblots, using antiserum raised against a recombinant enolase fusion protein. Because C. albicans enolase is abundantly produced duri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.7.1772-1779.1995
更新日期:1995-04-01 00:00:00
abstract::Rhizobium sp. strain NGR234 produces a large family of lipochitooligosaccharide Nod factors carrying specific substituents. Among them are 3-O- (or 4-O-) and 6-O-carbamoyl groups, an N-methyl group, and a 2-O-methylfucose residue which may bear either 3-O-sulfate or 4-O-acetyl substitutions. Investigations on the gene...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.16.5087-5093.1997
更新日期:1997-08-01 00:00:00
abstract::The binding of Bacteroides intermedius VPI 8944 to human fibrinogen has been characterized. The binding is time dependent, at least partially reversible, saturable, and specific. On an average, a maximum of 3,500 fibrinogen molecules bind per bacterial cell, with a dissociation constant of 1.7 X 10(-11) M. These bacte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.2.623-628.1985
更新日期:1985-08-01 00:00:00
abstract::AdnA is a transcription factor in Pseudomonas fluorescens that affects flagellar synthesis, biofilm formation, and sand adhesion. To identify the AdnA regulon, we used a promoterless Tn5-lacZ element to study the phenotypes of insertion mutants in the presence and absence of AdnA. Of 12,000 insertions, we identified s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.2.453-460.2003
更新日期:2003-01-01 00:00:00
abstract::Escherichia coli produces polysaccharide capsules that, based on their mechanisms of synthesis and assembly, have been classified into four groups. The group 4 capsule (G4C) polysaccharide is frequently identical to that of the cognate lipopolysaccharide O side chain and has, therefore, also been termed the O-antigen ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.15.5259-5266.2005
更新日期:2005-08-01 00:00:00
abstract::A 22.4-kilobase DNA fragment containing the tmrA7-amyR2-amyE+-tmrB+-aroI+ region of the Bacillus subtilis N7 chromosomal DNA was cloned into a recombinant B. subtilis bacteriophage, p11-AA248. The amyE+-tmrB+ gene region, approximately 12.6 kilobases, in the phage genome was amplified in a tunicamycin-resistant (Tmr) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.2.549-556.1986
更新日期:1986-02-01 00:00:00
abstract::We report the first example of a gene, hmp, encoding a soluble flavohemoglobin in Escherichia coli K-12, which is up-regulated by paraquat in a SoxRS-independent manner. Unlike what is found for other paraquat-inducible genes, high concentrations of paraquat (200 microM) were required to increase the level of hmp expr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.10.3164-3170.1997
更新日期:1997-05-01 00:00:00
abstract::The virD locus of Agrobacterium tumefaciens Ti plasmid pTiA6 was sequenced. Computer analysis of the sequence indicated five possible open reading frames (ORFs) within this locus. Two additional ORFs were identified distal to this locus. However, only two polypeptides of apparent molecular masses 16 and 56 kilodaltons...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.11.5035-5045.1987
更新日期:1987-11-01 00:00:00
abstract::Acholeplasma laidlawii cells grown with oleic acid produced much more colored carotenoids than did cells grown with elaidic acid. The amount of carotenoids was decreased 80 to 90% by growing the cells with 0.05 M propionate, resulting in a marked increase in the mobility of both 5-doxylstearate and 12-doxylstearate in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.3.944-948.1979
更新日期:1979-12-01 00:00:00
abstract::The BacA protein is essential for the long-term survival of Sinorhizobium meliloti and Brucella abortus within acidic compartments in plant and animal cells, respectively. Since both the S. meliloti and B. abortus bacA mutants have an increased resistance to bleomycin, it was hypothesized that BacA was a transporter o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.20.5625-5632.2002
更新日期:2002-10-01 00:00:00
abstract::In the yeast Saccharomyces cerevisiae, IRE1 encodes a bifunctional protein with transmembrane kinase and endoribonuclease activities. HAC1 encodes a transcription factor which has a basic leucine zipper domain. Both gene products play a crucial role in the unfolded protein response. Mutants in which one of these genes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.183.17.4985-4993.2001
更新日期:2001-09-01 00:00:00
abstract::The par genes of unit-copy plasmids P1 and F promote equitable distribution of plasmid copies to daughter cells and can be considered to be functional analogs of eucaryotic centromeres. Composite plasmids were constructed which carry either two functional P1 par regions or one F and one P1 region. Unlike dicentric chr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.2.742-745.1984
更新日期:1984-05-01 00:00:00
abstract::Small nuclear ribonucleoprotein (snRNP) particles play an important role in the processing of pre-mRNA. snRNPs have been identified immunologically in a variety of cells, but none have ever been observed in prokaryotic systems. This report provides the first evidence for the presence of snRNP-like components in two ty...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.7.1871-1878.1993
更新日期:1993-04-01 00:00:00
abstract::The regulation of Agrobacterium tumefaciens heat shock genes involves a transcriptional activator (RpoH) and repressor elements (HrcA-CIRCE). Using proteome analysis and mutants in these control elements, we show that the heat shock induction of 32 (out of 56) heat shock proteins is independent of RpoH and HrcA. These...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.6.1772-1778.2002
更新日期:2002-03-01 00:00:00
abstract::Haight, Rodger D. (Oregon State University, Corvallis), and Richard Y. Morita. Thermally induced leakage from Vibrio marinus, an obligately psychrophilic bacterium. J. Bacteriol. 92:1388-1393. 1966.-Leakage of various cellular components into the surrounding menstruum occurred when Vibrio marinus was subjected to temp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.5.1388-1393.1966
更新日期:1966-11-01 00:00:00
abstract::The fine structure of Paracoccidioides brasiliensis undergoing temperature-dependent transformation from mycelium to yeast and vice versa (M right harpoon over left harpoon Y) was studied. The transitional form to mycelium from the yeast appears as an elongated bud that extends from the yeast and which has a mixture o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.2.1076-1082.1969
更新日期:1969-11-01 00:00:00
abstract::An RNA polymerase mutant with a single-base-pair change in the rpoC gene affects chromosome initiation control. The mutation, which is recessive, is a G to A transition leading to the substitution of aspartate for glycine at amino acid residue 1033 in the RNA polymerase beta' subunit. The chromosome copy number is inc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.16.5200-5206.1991
更新日期:1991-08-01 00:00:00
abstract::Calcium-dependent exonuclease activity was produced by sporulating cells of Bacillus subtilis 168. Nuclease activity was released into the culture medium at approximately the same time as sporulation proteases, and production of these enzymes was tied to DNA replication. Results suggest that nuclease production is a f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.768-770.1982
更新日期:1982-02-01 00:00:00
abstract::The transcriptional regulatory protein Rgg coordinates amino acid catabolism and virulence factor expression in Streptococcus pyogenes. We used a proteomic approach to compare cytoplasmic proteins isolated from S. pyogenes wild-type strain NZ131 (serotype M49) to proteins isolated from an rgg mutant strain during the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.21.7091-7099.2004
更新日期:2004-11-01 00:00:00
abstract::In a previous study (J. O'Rear, L. Alberti, and R. M. Harshey, J. Bacteriol. 174:6125-6137, 1992) we reported the isolation of several transposon mutants of Serratia marcescens 274 that were defective either in swarming alone or in both swimming and swarming motility. All the nonflagellate (Fla-) mutants, while defect...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.4.987-991.1995
更新日期:1995-02-01 00:00:00
abstract::Level of competence reached by Bacillus subtilis 168 lysogenic for temperate phage phi 105 was reduced compared to that reached by nonlysogenic cells. This effect was probably related to an alteration of the bacterial surface. Deoxyribonucleic acid extracted from phi 105 lysogenic bacteria was used to transform other ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.3.874-877.1969
更新日期:1969-06-01 00:00:00
abstract::A naturally occurring constitutive macrolide-lincosamide-streptogramin B (MLS) resistance plasmid, pNE131, from Staphylococcus epidermidis was chosen to study the molecular basis of constitutive expression. Restriction and functional maps of pNE131 are presented along with the nucleotide sequence of ermM, the gene whi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.2.479-483.1986
更新日期:1986-05-01 00:00:00
abstract::Vibrio cholerae is a motile bacterium responsible for the disease cholera, and motility has been hypothesized to be inversely regulated with virulence. We examined the transcription profiles of V. cholerae strains containing mutations in flagellar regulatory genes (rpoN, flrA, flrC, and fliA) by utilizing whole-genome...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00949-09
更新日期:2009-11-01 00:00:00
abstract::In Escherichia coli, repression of the aceBAK operon is mediated by the IclR protein. We used an in vitro oligonucleotide selection technique to determine the consensus recognition sequence for MR. Mutational analysis confirmed the contribution of this sequence to repression in vivo and identified the -35 element of t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.13.3982-3984.1996
更新日期:1996-07-01 00:00:00