Copy number and the stability of 2-micron circle-based artificial plasmids of Saccharomyces cerevisiae.

Abstract:

:The copy number and stability of artificial 2-micron circle-based plasmids have been accurately measured in [Cir+] and [Cir0] strains of Saccharomyces cerevisiae. We conclude that (i) instability and copy number vary greatly from plasmid to plasmid; (ii) instability and copy number are negatively correlated--that is, high copy number is associated with low instability; (iii) it is difficult to reconcile this variability with a strict and direct system of copy number control; (iv) instabilities are much higher than expected from random partition and the observed copy numbers: this may imply partition which is less efficient than random. Even so, (v) the partitioning of 2-micron circle-like plasmids is more efficient than that of ARS-based plasmids, which hints at the existence of a system for the (inefficient) distribution of 2-micron circles.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Futcher AB,Cox BS

doi

10.1128/JB.157.1.283-290.1984

subject

Has Abstract

pub_date

1984-01-01 00:00:00

pages

283-90

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

157

pub_type

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