The virulence plasmid of Salmonella typhimurium is self-transmissible.

Abstract:

:Most isolates of Salmonella enterica serovar Typhimurium contain a 90-kb virulence plasmid. This plasmid is reported to be mobilizable but nonconjugative. However, we have determined that the virulence plasmid of strains LT2, 14028, and SR-11 is indeed self-transmissible. The plasmid of strain SL1344 is not. Optimal conjugation frequency requires filter matings on M9 minimal glucose plates with a recipient strain lacking the virulence plasmid. These conditions result in a frequency of 2.9 x 10(-4) transconjugants/donor. Matings on Luria-Bertani plates, liquid matings, or matings with a recipient strain carrying the virulence plasmid reduce the efficiency by up to 400-fold. Homologs of the F plasmid conjugation genes are physically located on the virulence plasmid and are required for the conjugative phenotype.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ahmer BM,Tran M,Heffron F

doi

10.1128/JB.181.4.1364-1368.1999

subject

Has Abstract

pub_date

1999-02-01 00:00:00

pages

1364-8

issue

4

eissn

0021-9193

issn

1098-5530

journal_volume

181

pub_type

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