Addition of Escherichia coli K-12 growth observation and gene essentiality data to the EcoCyc database.

abstract：:Holins are a diverse group of small integral membrane proteins elaborated by bacteriophages to lyse bacterial hosts and effect release of progeny phages in a precisely timed manner. Recently, the holin S gene of phage lambda was overexpressed and the holin protein was purified to homogeneity by means of an oligohistid...

journal_title：Journal of bacteriology

authors： Smith DL,Young R

更新日期：1998-08-01 00:00:00

abstract：UNLABELLED:Pupylation is a posttranslational modification peculiar to actinobacteria wherein proteins are covalently modified with a small protein called the prokaryotic ubiquitin-like protein (Pup). Like ubiquitination in eukaryotes, this phenomenon has been associated with proteasome-mediated protein degradation in m...

journal_title：Journal of bacteriology

authors： Compton CL,Fernandopulle MS,Nagari RT,Sello JK

更新日期：2015-09-01 00:00:00

abstract：:In the nosocomial opportunistic pathogen Acinetobacter baumannii, RecA-dependent mutagenesis, which causes antibiotic resistance acquisition, is linked to the DNA damage response (DDR). Notably, unlike the Escherichia coli paradigm, recA and DDR gene expression in A. baumannii is bimodal. Namely, there is phenotypic v...

journal_title：Journal of bacteriology

authors： Ching C,Gozzi K,Heinemann B,Chai Y,Godoy VG

更新日期：2017-05-09 00:00:00

abstract：:Several spontaneous cya and crp mutants of Escherichia coli have been selected as clones simultaneously resistant to phage lambda and nalidixic acid and characterized. Both cya and crp mutants have been found to grow as cocci with increased doubling times. They have increased resistance to some mutagens (methylmethane...

journal_title：Journal of bacteriology

authors： Kumar S

更新日期：1976-02-01 00:00:00

abstract：:The SasG surface protein of Staphylococcus aureus has been shown to promote the formation of biofilm. SasG comprises an N-terminal A domain and repeated B domains. Here we demonstrate that SasG is involved in the accumulation phase of biofilm, a process that requires a physiological concentration of Zn(2+). The B doma...

journal_title：Journal of bacteriology

authors： Geoghegan JA,Corrigan RM,Gruszka DT,Speziale P,O'Gara JP,Potts JR,Foster TJ

更新日期：2010-11-01 00:00:00

abstract：:An approach originally designed to identify functional origins of conjugative transfer (oriT or mob) in a bacterial genome (J. A. Herrera-Cervera, J. M. Sanjuán-Pinilla, J. Olivares, and J. Sanjuán, J. Bacteriol. 180:4583-4590, 1998) was modified to improve its reliability and prevent selection of undesired false mob ...

journal_title：Journal of bacteriology

authors： Pérez-Mendoza D,Domínguez-Ferreras A,Muñoz S,Soto MJ,Olivares J,Brom S,Girard L,Herrera-Cervera JA,Sanjuán J

更新日期：2004-09-01 00:00:00

abstract：:Forty years ago, Coulter and Talalay (A. W. Coulter and P. Talalay, J. Biol. Chem. 243:3238-3247, 1968) established the oxygenase-dependent pathway for the degradation of testosterone by aerobes. The oxic testosterone catabolic pathway involves several oxygen-dependent reactions and is not available for anaerobes. Sin...

journal_title：Journal of bacteriology

authors： Leu YL,Wang PH,Shiao MS,Ismail W,Chiang YR

更新日期：2011-09-01 00:00:00

abstract：:Two mutations concerned with mannan biosynthesis in the yeast Saccharomyces cerevisiae have been mapping. The mnn2 mutation, which affects the addition to the polysaccharide backbone of the first side-chain D-mannose unit in alpha1-leads to2 linkage, was located on chromosome II linked to the centromere and the gall l...

journal_title：Journal of bacteriology

authors： Ballou DL

更新日期：1975-08-01 00:00:00

abstract：:Maintaining cell envelope integrity is critical for bacterial survival, including bacteria living in a complex and dynamic environment such as the human oral cavity. Streptococcus mutans, a major etiological agent of dental caries, uses two-component signal transduction systems (TCSTSs) to monitor and respond to vario...

journal_title：Journal of bacteriology

authors： Suntharalingam P,Senadheera MD,Mair RW,Lévesque CM,Cvitkovitch DG

更新日期：2009-05-01 00:00:00

abstract：:The central metabolic model for Geobacter sulfurreducens included a single pathway for the biosynthesis of isoleucine that was analogous to that of Escherichia coli, in which the isoleucine precursor 2-oxobutanoate is generated from threonine. 13C labeling studies performed in G. sulfurreducens indicated that this pat...

journal_title：Journal of bacteriology

authors： Risso C,Van Dien SJ,Orloff A,Lovley DR,Coppi MV

更新日期：2008-04-01 00:00:00

abstract：:Here we have characterized the Rickettsia prowazekii RP534 protein, a homologue of the Pseudomonas aeruginosa ExoU phospholipase A (PLA) secreted cytotoxin. Our studies showed that purified recombinant RP534 PLA possessed the predicted PLA(2) and lyso-PLA(2) activities based on what has been published for P. aeruginos...

journal_title：Journal of bacteriology

authors： Housley NA,Winkler HH,Audia JP

更新日期：2011-09-01 00:00:00

abstract：:Archaeal transcriptional machinery is similar to that of eukaryotes. We studied the fates of various components of the Sulfolobus solfataricus transcriptional apparatus under different stresses and found that in cells incubated at 90 degrees C for 1 h, transcription factor E (TFE) is selectively depleted, but its mRNA...

journal_title：Journal of bacteriology

authors： Iqbal J,Qureshi SA

更新日期：2010-06-01 00:00:00

abstract：:Heterokaryons of Mucor racemosus were produced by fusion of spheroplasts from two auxotrophic strains of the fungus. Germinated sporangiospores were converted to spheroplasts by using commercial chitinase and Myxobacter AL-1 chitosanase. Spheroplasts from the auxotrophic strains were mixed in a buffered Ca(NO3)2 solut...

journal_title：Journal of bacteriology

authors： Genthner FJ,Borgia PT

更新日期：1978-04-01 00:00:00

abstract：:Bacillus brevis 47 contains two cell wall proteins termed the outer wall protein (OWP) and middle wall protein (MWP), each of which forms hexagonal arrays in the cell wall. A 6-kilobase BglII-BclI fragment of B. brevis 47 DNA cloned into Bacillus subtilis with a derivative of pHW1 as a vector directed the synthesis of...

journal_title：Journal of bacteriology

authors： Tsuboi A,Uchihi R,Tabata R,Takahashi Y,Hashiba H,Sasaki T,Yamagata H,Tsukagoshi N,Udaka S

更新日期：1986-10-01 00:00:00

abstract：:Regulation of isoleucine, valine, and leucine biosynthesis and isoleucyl-, valyl-, and leucyl-transfer ribonucleic acid (tRNA) synthetase formation was examined in two mutant strains of Escherichia coli. One mutant was selected for growth resistance to the isoleucine analogue, ketomycin, and the other was selected for...

journal_title：Journal of bacteriology

authors： Jackson J,Williams LS,Umbarger HE

更新日期：1974-12-01 00:00:00

abstract：:In many bacteria, the ferric uptake regulator (Fur) protein plays a central role in the regulation of iron uptake genes. Because iron figures prominently in the agriculturally important symbiosis between soybean and its nitrogen-fixing endosymbiont Bradyrhizobium japonicum, we wanted to assess the role of Fur in the i...

journal_title：Journal of bacteriology

authors： Benson HP,LeVier K,Guerinot ML

更新日期：2004-03-01 00:00:00

abstract：:The composition of the molecular species of acidic phospholipids in Escherichia coli B during the late exponential growth phase at 37 degrees C was determined. Two phosphatidyl groups of cardiolipin, the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of cardiolipin, were isolated by limited hydrolysis with p...

journal_title：Journal of bacteriology

authors： Yokota K,Kanamoto R,Kito M

更新日期：1980-03-01 00:00:00

abstract：:The TatA and TatB proteins are essential components of the twin arginine protein translocation pathway in Escherichia coli. C-terminal truncation analysis of the TatA protein revealed that a plasmid-expressed TatA protein shortened by 40 amino acids is still fully competent to support protein translocation. Similar tr...

journal_title：Journal of bacteriology

authors： Lee PA,Buchanan G,Stanley NR,Berks BC,Palmer T

更新日期：2002-11-01 00:00:00

abstract：:We report a phenomenon similar to catabolite repression in Rhizobium meliloti. Succinate, which allows the highest observed rate of growth of R. meliloti, caused an immediate reduction of beta-galactosidase activity when added to cells growing in lactose. A Lac- mutant was unaltered in nodulation and nitrogen fixation...

journal_title：Journal of bacteriology

authors： Ucker DS,Signer ER

更新日期：1978-12-01 00:00:00

abstract：:Five clostridial species were found to ferment gluconate via 2-keto-3-deoxygluconate which subsequently is phosphorylated to yield 2-keto-3-deoxy-6-phosphogluconate (KDPG). This compound is then cleaved by KDPG aldolase. ...

journal_title：Journal of bacteriology

authors： Bender R,Andreesen JR,Gottschalk G

更新日期：1971-08-01 00:00:00

abstract：:Immunity to leptospirosis is principally humorally mediated and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. The only protective antigen identified to date is the leptospiral lipopolysaccharide (LPS), which biochemically resembles typical gram-negative LPS but has greatly reduc...

journal_title：Journal of bacteriology

authors： Mitchison M,Bulach DM,Vinh T,Rajakumar K,Faine S,Adler B

更新日期：1997-02-01 00:00:00

abstract：:Penicillin-binding proteins (PBPs), although characterized from several organisms, have so far not been studied in mycobacteria. The present study is the first characterization of a PBP from Mycobacterium smegmatis. The PBP was purified by solubilization of the membranes with Triton X-100 and successive chromatography...

journal_title：Journal of bacteriology

authors： Basu J,Chattopadhyay R,Kundu M,Chakrabarti P

更新日期：1992-07-01 00:00:00

abstract：:Nagai, Susumu (National Women's University, Nara, Japan). Differential reduction of tellurite by growing colonies of normal yeast and respiration-deficient mutants. J. Bacteriol. 90:220-222. 1965.-A differential reduction of sodium tellurite was observed between normal and respiration-deficient mutant colonies of seve...

journal_title：Journal of bacteriology

authors： Nagai S

更新日期：1965-07-01 00:00:00

abstract：:DNA damage-inducible DNA replication in SOS-induced Escherichia coli cells, termed inducible stable DNA replication (iSDR), has previously been shown to require either the RecBCD or the RecE pathway of homologous recombination for initiation. Here, we demonstrate that recB recC sbcC quadruple mutant cells are capable ...

journal_title：Journal of bacteriology

authors： Asai T,Kogoma T

更新日期：1994-11-01 00:00:00

abstract：:Escherichia coli strains mutant in the starvation gene cstC grow normally in a mineral salts medium but are impaired in utilizing amino acids as nitrogen sources. They are also compromised in starvation survival, where amino acid catabolism is important. The cstC gene encodes a 406-amino-acid protein that closely rese...

journal_title：Journal of bacteriology

authors： Fraley CD,Kim JH,McCann MP,Matin A

更新日期：1998-08-01 00:00:00

abstract：:In Salmonella enterica serovar Typhimurium, the stationary-phase sigma factor sigma(S) (RpoS) is required for virulence, stress resistance, biofilm formation, and development of the rdar morphotype. This morphotype is a multicellular behavior characterized by expression of the adhesive extracellular matrix components ...

journal_title：Journal of bacteriology

authors： Robbe-Saule V,Jaumouillé V,Prévost MC,Guadagnini S,Talhouarne C,Mathout H,Kolb A,Norel F

更新日期：2006-06-01 00:00:00

abstract：:Poly-beta-hydroxybutyrate (PHB) synthesis in a cyanobacterium, Synechococcus sp. strain MA19, is controlled at the enzyme level and is dependent on the C/N balance in the culture medium. The control involves at least two enzymes. The first enzyme is PHB synthase. Little PHB synthase activity was detected in crude extr...

journal_title：Journal of bacteriology

authors： Miyake M,Kataoka K,Shirai M,Asada Y

更新日期：1997-08-01 00:00:00

abstract：:Penicillin-binding protein 2 (PBP-2) of Escherichia coli K-12 was purified by covalent affinity chromatography using 6-aminopenicillanic acid covalently coupled to carboxymethyl-Sepharose (6-APA-CM-Sepharose). Purification of PBP-2 was accomplished by prebinding the methoxy cephalosporin, cefoxitin, to the Triton X-10...

journal_title：Journal of bacteriology

authors： Curtis SJ,Strominger JL

更新日期：1981-01-01 00:00:00

abstract：:Western (immunoblot) analysis using Escherichia coli anti-RecA antiserum revealed that expression of the RecA protein of Pseudomonas aeruginosa PAO is induced upon exposure of the bacterium to UV irradiation or norfloxacin, a quinolone related to nalidixic acid. ...

journal_title：Journal of bacteriology

authors： Miller RV,Kokjohn TA

更新日期：1988-05-01 00:00:00

abstract：:The papA gene of the uropathogenic strain Escherichia coli J96, coding for the Pap pili subunit, was subjected to DNA sequencing, and found to code for an 185-amino acid-long polypeptide with a 22-amino acid-long signal peptide. Here we present the primary sequence, the hydrophilicity profile, and the predicted polype...

journal_title：Journal of bacteriology

authors： Båga M,Normark S,Hardy J,O'Hanley P,Lark D,Olsson O,Schoolnik G,Falkow S

更新日期：1984-01-01 00:00:00