A quorum-sensing system in the free-living photosynthetic bacterium Rhodobacter sphaeroides.

Abstract:

:Rhodobacter sphaeroides is a free-living, photoheterotrophic bacterium known for its genomic and metabolic complexity. We have discovered that this purple photosynthetic organism possesses a quorum-sensing system. Quorum sensing occurs in a number of eukaryotic host-associated gram-negative bacteria. In these bacteria there are two genes required for quorum sensing, the luxR and luxI homologs, and there is an acylhomoserine lactone signal molecule synthesized by the product of the luxI homolog. In R. sphaeroides, synthesis of a novel homoserine lactone signal, 7,8-cis-N-(tetradecenoyl)homoserine lactone, is directed by a luxI homolog termed cerI. Two open reading frames immediately upstream of cerI are proposed to be components of the quorum-sensing system. The first of these is a luxR homolog termed cerR, and the second is a small open reading frame of 159 bp. Inactivation of cerI in R. sphaeroides results in mucoid colony formation on agar and formation of large aggregates of cells in liquid cultures. Clumping of CerI mutants in liquid culture is reversible upon addition of the acylhomoserine lactone signal and represents a phenotype unlike those controlled by quorum sensing in other bacteria.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Puskas A,Greenberg EP,Kaplan S,Schaefer AL

doi

10.1128/jb.179.23.7530-7537.1997

subject

Has Abstract

pub_date

1997-12-01 00:00:00

pages

7530-7

issue

23

eissn

0021-9193

issn

1098-5530

journal_volume

179

pub_type

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