Genetic selection and DNA sequences of 4.5S RNA homologs.

abstract：:Burkholderia cenocepacia strain K56-2, a representative of the Burkholderia cepacia complex, is part of the epidemic and clinically problematic ET12 lineage. The strain produced plant tissue watersoaking (ptw) on onion tissue, which is a plant disease-associated trait. Using plasposon mutagenesis, mutants in the ptw p...

journal_title：Journal of bacteriology

authors： Engledow AS,Medrano EG,Mahenthiralingam E,LiPuma JJ,Gonzalez CF

更新日期：2004-09-01 00:00:00

abstract：:Agrobacterium tumefaciens R10 was mutagenized by using the promoter probe transposon Tn5-gusA7, and a library of approximately 5,000 transcriptional fusions was screened for octopine-inducible patterns of gene expression. Twenty-one mutants carrying strongly inducible gusA fusions, 20 of which showed defects in the ca...

journal_title：Journal of bacteriology

authors： Cho K,Fuqua C,Martin BS,Winans SC

更新日期：1996-04-01 00:00:00

abstract：:The phoP-phoQ operon of Salmonella typhimurium is a member of the family of two-component regulatory systems and controls expression of the phoN gene that codes for nonspecific acid phosphatase and the genes involved in the pathogenicity of the bacterium. The phoP-phoQ operon of Escherichia coli was cloned on a plasmi...

journal_title：Journal of bacteriology

authors： Kasahara M,Nakata A,Shinagawa H

更新日期：1992-01-01 00:00:00

abstract：:Exogenous proline specifically stimulates the growth rate of enteric bacteria in media of inhibitory osmotic strength (J. H. B. Christian, Aust. J. Biol. Sci. 8:490-497, 1955). I observed that Salmonella typhimurium mutants which lack both of the previously known proline permeases (putP proP) are stimulated by proline...

journal_title：Journal of bacteriology

authors： Csonka LN

更新日期：1982-09-01 00:00:00

abstract：UNLABELLED:The Mycobacterium tuberculosis exported repetitive protein (RvErp) is a crucial virulence-associated factor as determined by its role in the survival and multiplication of mycobacteria in cultured macrophages and in vivo Although attempts have been made to understand the function of Erp protein, its exact ro...

journal_title：Journal of bacteriology

authors： Ganaie AA,Trivedi G,Kaur A,Jha SS,Anand S,Rana V,Singh A,Kumar S,Sharma C

更新日期：2016-09-22 00:00:00

abstract：:In Escherichia coli, the three branched-chain amino acid activating enzymes appear to be essential for multivalent repression of the isoleucine- and valine-forming enzymes. The results of experiments with a mutant, strain CU18, having an altered threonine deaminase, indicate that free isoleucine and some form of threo...

journal_title：Journal of bacteriology

authors： Wasmuth JJ,Umbarger HE

更新日期：1974-01-01 00:00:00

abstract：:We determined, under several growth conditions, the kinetics of mRNA synthesis from the four Pseudomonas putida pWW0 plasmid promoters involved in degradation of xylenes and methylbenzyl alcohols via toluates. Transcription by XylS of the meta-cleavage pathway operon promoter (Pm) for the metabolism of alkylbenzoates ...

journal_title：Journal of bacteriology

authors： Marqués S,Holtel A,Timmis KN,Ramos JL

更新日期：1994-05-01 00:00:00

abstract：:Xylella fastidiosa is a phytopathogenic bacterium that is responsible for diseases in many economically important crops. Although different strains have been studied, little is known about X. fastidiosa stress responses. One of the better characterized stress responses in bacteria is the heat shock response, which ind...

journal_title：Journal of bacteriology

authors： Koide T,Vêncio RZ,Gomes SL

更新日期：2006-08-01 00:00:00

abstract：:XbaI digestion and pulsed-field gel electrophoresis of the genome of Salmonella typhimurium LT2 yields 24 fragments: 23 fragments (total size, 4,807 kb) are from the chromosome, and one fragment (90 kb) is from the virulence plasmid pSLT. Some of the 23 fragments from the chromosome were located on the linkage map by ...

journal_title：Journal of bacteriology

authors： Liu SL,Sanderson KE

更新日期：1992-03-01 00:00:00

abstract：:The galP1 promoter is responsible for galactose-dependent, glucose-sensitive transcription of the galactose utilization operon of Streptomyces coelicolor and Streptomyces lividans. We describe the characterization of mutations that were positioned directly upstream of the apparent transcription start site of galP1 and...

journal_title：Journal of bacteriology

authors： Mattern SG,Brawner ME,Westpheling J

更新日期：1993-03-01 00:00:00

abstract：:A procedure is described that uses an indicator plasmid (pSC201) to identify cells in a bacterial population that have been co-transformed with a second plasmid lacking detectable phenotypic properties. Under appropriate conditions of indirect selection, between 50 and 85% of transformants carrying the indicator plasm...

journal_title：Journal of bacteriology

authors： Kretschmer FJ,Chang AC,Cohen SN

更新日期：1975-10-01 00:00:00

abstract：:Evolution by natural selection occurs in cultures of Escherichia coli maintained under carbon starvation stress. Mutants of increased fitness express a growth advantage in stationary phase (GASP) phenotype, enabling them to grow and displace the parent as the majority population. The first GASP mutation was identified...

journal_title：Journal of bacteriology

authors： Zinser ER,Kolter R

更新日期：2000-08-01 00:00:00

abstract：:In streptomycete anthracycline biosynthetic gene clusters, small open reading frames are located just upstream of minimal polyketide synthase genes. aknX is such a gene found in the aklavinone-aclacinomycin biosynthetic gene cluster of Streptomyces galilaeus. In order to identify its function, the aknX gene was expres...

journal_title：Journal of bacteriology

authors： Chung JY,Fujii I,Harada S,Sankawa U,Ebizuka Y

更新日期：2002-11-01 00:00:00

abstract：:Structural studies were carried out on lipoteichoic acids obtained from defatted cells of 10 Bacillus strains by phenol-water partition followed by chromatography on DEAE-Sephacel and Octyl-Sepharose columns. A group of the tested bacteria (group A), Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus, was...

journal_title：Journal of bacteriology

authors： Iwasaki H,Shimada A,Ito E

更新日期：1986-08-01 00:00:00

abstract：:Perry, Jerome J. (North Carolina State University, Raleigh), and James B. Evans. Oxidation and assimilation of carbohydrates by Micrococcus sodonensis. J. Bacteriol. 91:33-38. 1966.-Micrococcus sodonensis is a biotin-requiring strict aerobe that cannot utilize carbohydrates as sole sources of carbon and energy. Howeve...

journal_title：Journal of bacteriology

authors： Perry JJ,Evans JB

更新日期：1966-01-01 00:00:00

abstract：:We have isolated transposon insertions in the lacA gene encoding an endogenous beta-galactosidase of Bacillus subtilis. Upstream of the putative operon containing lacA is a negative regulator, lacR, which encodes a product related to a family of regulators that includes the lactose repressor, lacI, of Escherichia coli...

journal_title：Journal of bacteriology

authors： Daniel RA,Haiech J,Denizot F,Errington J

更新日期：1997-09-01 00:00:00

abstract：:A highly efficient protoplast transformation system for Streptococcus faecalis has been developed by systematically optimizing different parameters. Up to 10(6) transformants per micrograms of DNA were consistently obtained within 3 days, and cell wall regeneration of protoplasts was virtually 100%. A systematic searc...

journal_title：Journal of bacteriology

authors： Wirth R,An FY,Clewell DB

更新日期：1986-03-01 00:00:00

abstract：:An exonuclease III-deficient strain of Escherichia coli K-12, BW2001 (xthA11), was unable to perform rapid repair of X-ray-induced deoxyribonucleic acid single-strand breaks and appeared to have a defect in the priming of the 3'-termini necessary for initiation of repair synthesis at the breaks. This defect cannot be ...

journal_title：Journal of bacteriology

authors： Seeberg E,Steinum AL

更新日期：1980-03-01 00:00:00

abstract：:Glucose-1-(14)C and acetylglucosamine-1-(14)C were added singly and together with equal amounts of the unlabeled reciprocal to Brain Heart Infusion and used for the culture of Streptococcus pyogenes. The labeling pattern of the rhamnose, glucosamine, and muramic acid in the cell wall supported an intermediary role for...

journal_title：Journal of bacteriology

authors： Barkulis SS,Boltralik JJ,Hankin H,Heymann H

更新日期：1967-10-01 00:00:00

abstract：:mRNA levels from the bop (bacterio-opsin), brp (bacterio-opsin-related protein), and bat (bacterio-opsin activator) genes in wild-type Halobacterium halobium and two bacteriorhodopsin-overproducing mutants (ET1001 and II-7) were quantitated under conditions in which oxygen levels were steadily depleted and then cultur...

journal_title：Journal of bacteriology

authors： Shand RF,Betlach MC

更新日期：1994-03-01 00:00:00

abstract：:Parallel studies were performed with methionineless derivatives of Escherichia coli 15 T(-) and Bacillus megaterium KM: T(-). Methylated bases are present in the total cell ribonucleic acid (RNA) of B. megaterium. The level of RNA methylation in E. coli is about 60% greater than that in B. megaterium. Although E. coli...

journal_title：Journal of bacteriology

authors： Wachsman JT,Irwin V

更新日期：1970-11-01 00:00:00

abstract：:The competence-related phenotypes of mutations in each of the four open reading frames associated with the comE locus of Bacillus subtilis are described. comEA and comEC are required for transformability, whereas the products of comEB and of the overlapping comER, which is transcribed in the reverse direction, are dis...

journal_title：Journal of bacteriology

authors： Inamine GS,Dubnau D

更新日期：1995-06-01 00:00:00

abstract：:Proteins encoded by plasmid DNA are specifically labeled in UV-irradiated cells of Escherichia coli carrying recA and uvrA mutations because extensive degradation of the chromosome DNA occurs concurrently with amplification of plasmid DNA. ...

journal_title：Journal of bacteriology

authors： Sancar A,Hack AM,Rupp WD

更新日期：1979-01-01 00:00:00

abstract：:Biofilm formation was repressed by glucose in several species of Enterobacteriaceae. In Escherichia coli, this effect was mediated at least in part by cyclic AMP (cAMP)-cAMP receptor protein. A temporal role for cAMP in biofilm development was indicated by the finding that glucose addition after approximately 24 h fai...

journal_title：Journal of bacteriology

authors： Jackson DW,Simecka JW,Romeo T

更新日期：2002-06-01 00:00:00

abstract：:Two different mutations (pabA and pabB) affecting 4-aminobenzoate biosynthesis were obtained in strains of Escherichia coli lacking chorismate mutase and anthranilate synthetase activity, thus allowing study of the pathway of biosynthesis of 4-aminobenzoate by use of cell extracts of strains carrying the pab mutations...

journal_title：Journal of bacteriology

authors： Huang M,Gibson F

更新日期：1970-06-01 00:00:00

abstract：:We examined 14 multiresistant and 8 ampicillin- or tetracycline-resistant Haemophilus influenzae isolates and 4 ampicillin-resistant H. parainfluenzae isolates for plasmid deoxyribonucleic acid. Sixteen strains carried plasmids. Both "plasmid-free" and plasmid-carrying isolates transferred the antibiotic resistance by...

journal_title：Journal of bacteriology

authors： Roberts MC,Smith AL

更新日期：1980-10-01 00:00:00

abstract：:We previously elucidated the global transcriptional responses of Escherichia coli to the nitrosating agent S-nitrosoglutathione (GSNO) in both aerobic and anaerobic chemostats, demonstrated the expression of nitric oxide (NO)-protective mechanisms, and obtained evidence of critical thiol nitrosation. The present study...

journal_title：Journal of bacteriology

authors： Pullan ST,Gidley MD,Jones RA,Barrett J,Stevanin TM,Read RC,Green J,Poole RK

更新日期：2007-03-01 00:00:00

abstract：:Stalk formation is a novel pattern of multicellular organization. Yeast cells which survive UV irradiation form colonies that grow vertically to form very long (0.5 to 3.0 cm) and thin (0.5 to 4 mm in diameter) multicellular structures. We describe the conditions required to obtain these stalk-like structures reproduc...

journal_title：Journal of bacteriology

authors： Engelberg D,Mimran A,Martinetto H,Otto J,Simchen G,Karin M,Fink GR

更新日期：1998-08-01 00:00:00

abstract：:Gilden, R. V. (Wistar Institute, Philadelphia, Pa.), and R. I. Carp. Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells. J. Bacteriol. 91:1295-1297. 1966.-Synthesis of the simian virus 40 (SV40) T antigen in primary African green monkey kidney cells was abolis...

journal_title：Journal of bacteriology

authors： Gilden RV,Carp RI

更新日期：1966-03-01 00:00:00

abstract：:Erythrobacter litoralis has been known as a bacteriochlorophyll a-containing, aerobic, anoxygenic, phototrophic bacterium. Here we announce the complete genome sequence of E. litoralis HTCC2594, which is devoid of phototrophic potential. E. litoralis HTCC2594, isolated by dilution-to-extinction culturing from seawater...

journal_title：Journal of bacteriology

authors： Oh HM,Giovannoni SJ,Ferriera S,Johnson J,Cho JC

更新日期：2009-04-01 00:00:00