The OxyR regulon in nontypeable Haemophilus influenzae.

Abstract:

:Nontypeable Haemophilus influenzae (NTHi) is a gram-negative bacterium and a common commensal organism of the upper respiratory tract in humans. NTHi causes a number of diseases, including otitis media, sinusitis, conjunctivitis, exacerbations of chronic obstructive pulmonary disease, and bronchitis. During the course of colonization and infection, NTHi must withstand oxidative stress generated by insult due to multiple reactive oxygen species produced endogenously by other copathogens and by host cells. Using an NTHi-specific microarray containing oligonucleotides representing the 1821 open reading frames of the recently sequenced NTHi isolate 86-028NP, we have identified 40 genes in strain 86-028NP that are upregulated after induction of oxidative stress due to hydrogen peroxide. Further comparisons between the parent and an isogenic oxyR mutant identified a subset of 11 genes that were transcriptionally regulated by OxyR, a global regulator of oxidative stress. Interestingly, hydrogen peroxide induced the OxyR-independent upregulation of expression of the genes encoding components of multiple iron utilization systems. This finding suggested that careful balancing of levels of intracellular iron was important for minimizing the effects of oxidative stress during NTHi colonization and infection and that there are additional regulatory pathways involved in iron utilization.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Harrison A,Ray WC,Baker BD,Armbruster DW,Bakaletz LO,Munson RS Jr

doi

10.1128/JB.01040-06

subject

Has Abstract

pub_date

2007-02-01 00:00:00

pages

1004-12

issue

3

eissn

0021-9193

issn

1098-5530

pii

JB.01040-06

journal_volume

189

pub_type

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