Characterization of two methanopterin biosynthesis mutants of Methylobacterium extorquens AM1 by use of a tetrahydromethanopterin bioassay.

Abstract:

:An enzymatic assay was developed to measure tetrahydromethanopterin (H(4)MPT) levels in wild-type and mutant cells of Methylobacterium extorquens AM1. H(4)MPT was detectable in wild-type cells but not in strains with a mutation of either the orf4 or the dmrA gene, suggesting a role for these two genes in H(4)MPT biosynthesis. The protein encoded by orf4 catalyzed the reaction of ribofuranosylaminobenzene 5'-phosphate synthase, the first committed step of H(4)MPT biosynthesis. These results provide the first biochemical evidence for H(4)MPT biosynthesis genes in bacteria.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Rasche ME,Havemann SA,Rosenzvaig M

doi

10.1128/jb.186.5.1565-1570.2004

keywords:

subject

Has Abstract

pub_date

2004-03-01 00:00:00

pages

1565-70

issue

5

eissn

0021-9193

issn

1098-5530

journal_volume

186

pub_type

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