Localization of c-di-GMP-binding protein with the linear terminal complexes of Acetobacter xylinum.

Abstract:

:Specific labeling of a single row of cellulose-synthesizing complexes (terminal complexes, TC subunits, TCs, or TC arrays) in Acetobacter xylinum by antibodies raised against a 93-kDa protein (the cyclic dignanylic acid-binding protein) has been demonstrated by using the sodium dodecyl sulfate (SDS)-freeze-fracture labeling (FRL) technique. The antibodies to the 93-kDa protein specifically recognized the TC subunits on the protoplasmic fracture (PF) face of the outer membrane in A. xylinum; however, nonlabeled TCs were also observed. Two types of TC subunits (particles or pits) are observed on the PF face of the outer membrane: (i) immunogold-labeled TCs showing a line of depressions (pits) with an indistinct particle array and (ii) nonlabeled TC subunits with a distinct single row of particle arrays. The evidence indicates that the labeling patterns differ with respect to the presence or absence of certain TC subunits remaining attached to the replica after SDS treatment. This suggests the presence of at least two TC components, one in the outer membrane and the other in the cytoplasmic membrane. If the TC component in the outer membrane is preferentially fractured and remains attached to the ectoplasmic fracture face (or outer leaflet) of the outer membrane, subsequent replica formation reveals a pit or depression with positive antibody labeling on the PF face of the outer membrane. If the TC component in the outer membrane remains with the PF face (or inner leaflet) of the outer membrane, the innermost TC component is removed during SDS treatment and labeling does not occur. SDS-FRL of TCs in A. xylinum has enabled us to provide the first topological molecular analysis of component proteins in a cellulose-synthesizing TC structure in a prokaryotic organism.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kimura S,Chen HP,Saxena IM,Brown RM Jr,Itoh T

doi

10.1128/JB.183.19.5668-5674.2001

keywords:

subject

Has Abstract

pub_date

2001-10-01 00:00:00

pages

5668-74

issue

19

eissn

0021-9193

issn

1098-5530

journal_volume

183

pub_type

杂志文章
  • Characterization of pilin genes from seven serologically defined prototype strains of Moraxella bovis.

    abstract::Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characteri...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.16.4875-4882.1994

    authors: Atwell JL,Tennent JM,Lepper AW,Elleman TC

    更新日期:1994-08-01 00:00:00

  • MICROBIAL METABOLISM OF AROMATIC COMPOUNDS. I. DECOMPOSITION OF PHENOLIC COMPOUNDS AND AROMATIC HYDROCARBONS BY PHENOL-ADAPTED BACTERIA.

    abstract::Tabak, Henry H. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), Cecil W. Chambers, and Paul W. Kabler. Microbial metabolism of aromatic carbon compounds. I. Decomposition of phenolic compounds and aromatic hydrocarbons by phenol-adapted bacteria. J. Bacteriol. 87:910-919. 1964.-Bacteria from soil and r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.4.910-919.1964

    authors: TABAK HH,CHAMBERS CW,KABLER PW

    更新日期:1964-04-01 00:00:00

  • Role of Sphingomonas sp. strain Fr1 PhyR-NepR-σEcfG cascade in general stress response and identification of a negative regulator of PhyR.

    abstract::The general stress response in Alphaproteobacteria was recently described to depend on the alternative sigma factor σ(EcfG), whose activity is regulated by its anti-sigma factor NepR. The response regulator PhyR, in turn, regulates NepR activity in a partner-switching mechanism according to which phosphorylation of Ph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06006-11

    authors: Kaczmarczyk A,Campagne S,Danza F,Metzger LC,Vorholt JA,Francez-Charlot A

    更新日期:2011-12-01 00:00:00

  • Regulation of the Salmonella typhimurium aroF gene in Escherichia coli.

    abstract::The Salmonella typhimurium aroF gene, encoding the tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase, was localized to a chromosomal PstI fragment by Southern blotting with an Escherichia coli aroF probe. This fragment was cloned by screening a plasmid library for complementation of an E. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2259-2266.1990

    authors: Muday GK,Herrmann KM

    更新日期:1990-05-01 00:00:00

  • Properties of acetate kinase isozymes and a branched-chain fatty acid kinase from a spirochete.

    abstract::Spirochete MA-2, which is anaerobic, ferments glucose, forming acetate as a major product. The spirochete also ferments (but does not utilize as growth substrates) small amounts of l-leucine, l-isoleucine, and l-valine, forming the branched-chain fatty acids isovalerate, 2-methylbutyrate, and isobutyrate, respectively...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Harwood CS,Canale-Parola E

    更新日期:1982-10-01 00:00:00

  • Oxygen does not directly regulate carotenoid biosynthesis in Rhodopseudomonas capsulata.

    abstract::We examined the role of bacteriochlorophyll synthesis on the regulation of carotenoid synthesis in Rhodopseudomonas capsulata. Strains capable of making bacteriochlorophyll accumulated greater amounts of carotenoids under low oxygen than they did under high oxygen. However, strains unable to produce bacteriochlorophyl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.3.1320-1321.1985

    authors: Biel AJ,Marrs BL

    更新日期:1985-06-01 00:00:00

  • Spectra of spontaneous growth-dependent and adaptive mutations at ebgR.

    abstract::A comparison of the spectra of spontaneous growth-dependent and adaptive mutations in ebgR shows that both spectra are dominated by insertion sequence (IS)-mediated mutations. The difference between growth-dependent mutations (61% IS mediated) and adaptive mutations (80% IS mediated) is highly significant (P < 0.0001)...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.4.1149-1155.1999

    authors: Hall BG

    更新日期:1999-02-01 00:00:00

  • Rabbit ileal loop response to strains of Clostridium perfringens.

    abstract::The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum was important in determining whether a given strain would provoke a response. When cultures were grown for 4 hr at 37 C...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.5.1560-1566.1968

    authors: Duncan CL,Sugiyama H,Strong DH

    更新日期:1968-05-01 00:00:00

  • Translation rates of individual codons are not correlated with tRNA abundances or with frequencies of utilization in Escherichia coli.

    abstract::We analyzed 12 individual codons, which differed widely with respect to the frequency of use in Escherichia coli and the abundance of the corresponding tRNAs, for their influence on the coupling between transcription and translation. This was probed by determining the effects of codon substitutions in the leader pepti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.11.5812-5816.1989

    authors: Bonekamp F,Dalbøge H,Christensen T,Jensen KF

    更新日期:1989-11-01 00:00:00

  • New aerobic benzoate oxidation pathway via benzoyl-coenzyme A and 3-hydroxybenzoyl-coenzyme A in a denitrifying Pseudomonas sp.

    abstract::A denitrifying Pseudomonas sp. is able to oxidize aromatic compounds compounds completely to CO2, both aerobically and anaerobically. It is shown that benzoate is aerobically oxidized by a new degradation pathway via benzoyl-coenzyme A (CoA) and 3-hydroxybenzoyl-CoA. The organism grew aerobically with benzoate, 3-hydr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.15.4851-4858.1993

    authors: Altenschmidt U,Oswald B,Steiner E,Herrmann H,Fuchs G

    更新日期:1993-08-01 00:00:00

  • Molecular cloning of the uhp region and evidence for a positive activator for expression of the hexose phosphate transport system of Escherichia coli.

    abstract::The uhp locus of Escherichia coli contains genes for the sugar phosphate transport system (uhpT) and the regulatory system which allows its induction by external glucose 6-phosphate (uhpRA). The uhp region was cloned onto high-copy-number plasmids, both from Uhp(+) plasmids of the Clarke-Carbon collection and from gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.3.1062-1070.1983

    authors: Shattuck-Eidens DM,Kadner RJ

    更新日期:1983-09-01 00:00:00

  • Characterization of mutations affecting the osmoregulated proU promoter of Escherichia coli and identification of 5' sequences required for high-level expression.

    abstract::Expression of the Escherichia coli proU operon, which encodes an efficient uptake system for the osmoprotectant glycine betaine, is strongly increased in cells grown at high osmolarity. We isolated 182 independent spontaneous mutants with elevated expression of the chromosomal phi(proV-lacZ) (Hyb2) fusion at low osmol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.2.801-809.1991

    authors: Lucht JM,Bremer E

    更新日期:1991-01-01 00:00:00

  • Recruitment of the ParG segregation protein to different affinity DNA sites.

    abstract::The segrosome is the nucleoprotein complex that mediates accurate plasmid segregation. In addition to its multifunctional role in segrosome assembly, the ParG protein of multiresistance plasmid TP228 is a transcriptional repressor of the parFG partition genes. ParG is a homodimeric DNA binding protein, with C-terminal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01630-08

    authors: Zampini M,Derome A,Bailey SE,Barillà D,Hayes F

    更新日期:2009-06-01 00:00:00

  • Purification of mycobacterial deoxyribonucleic acid.

    abstract::Impurities believed to be polysaccharides have been found in mycobacterial deoxyribonucleic acid (DNA) preparations. Agar-gel diffusion of the DNA preparations against concanavalin A indicated the presence of three polysaccharides and was used to follow the purification procedures. The polysaccharides appeared to be t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.3.1033-1039.1972

    authors: Hill EB,Wayne LG,Gross WM

    更新日期:1972-12-01 00:00:00

  • Genome sequence of the Corynebacterium pseudotuberculosis Cp316 strain, isolated from the abscess of a Californian horse.

    abstract::The bacterium Corynebacterium pseudotuberculosis is of major veterinary importance because it affects livestock, particularly sheep, goats, and horses, in several countries, including Australia, Brazil, the United States, and Canada, resulting in significant economic losses. In the present study, we describe the compl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01616-12

    authors: Ramos RT,Silva A,Carneiro AR,Pinto AC,Soares Sde C,Santos AR,Almeida SS,Guimarães LC,Aburjaile FF,Barbosa EG,Dorella FA,Rocha FS,Cerdeira LT,Barbosa MS,Tauch A,Edman J,Spier S,Miyoshi A,Schneider MP,Azevedo V

    更新日期:2012-12-01 00:00:00

  • Glutamate transport in Escherichia coli K-12: nonidentity of carriers mediating entry and exit.

    abstract::The exit of glutamate from Escherichia coli K-12 cells preloaded with the radioactive amino acid and its relation to the reaction of entry were studied. Experiments with cells preloaded to different intracellular concentrations of radioactive glutamate confirmed our earlier conclusion that glutamate exit was a first-o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.1.51-57.1973

    authors: Halpern YS,Barash H,Druck K

    更新日期:1973-01-01 00:00:00

  • Overproduction of the rbo gene product from Desulfovibrio species suppresses all deleterious effects of lack of superoxide dismutase in Escherichia coli.

    abstract::In an attempt to isolate the superoxide dismutase (SOD) gene from the anaerobic sulfate-reducing bacterium Desulfoarculus baarsii, a DNA fragment was isolated which functionally complemented an Escherichia coli mutant (sodA sodB) deficient in cytoplasmic SODs. This region carries two open reading frames with sequences...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.23.6736-6742.1996

    authors: Pianzzola MJ,Soubes M,Touati D

    更新日期:1996-12-01 00:00:00

  • Growth and survival of Mycoplasma neurolyticum in liquid media.

    abstract::Hottle, G. A. (Naval Biological Laboratory, University of California, Berkeley), and D. N. Wright. Growth and survival of Mycoplasma neurolyticum in liquid media. J. Bacteriol. 91:1834-1839. 1966.-Maximal growth of Mycoplasma neurolyticum (between 10(8) and 10(9) colony-forming units per ml) was obtained after 3 days ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.5.1834-1839.1966

    authors: Hottle GA,Wright DN

    更新日期:1966-05-01 00:00:00

  • Two family B DNA polymerases from Aeropyrum pernix, an aerobic hyperthermophilic crenarchaeote.

    abstract::DNA polymerase activities in fractionated cell extract of Aeropyrum pernix, a hyperthermophilic crenarchaeote, were investigated. Aphidicolin-sensitive (fraction I) and aphidicolin-resistant (fraction II) activities were detected. The activity in fraction I was more heat stable than that in fraction II. Two different ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.19.5984-5992.1999

    authors: Cann IK,Ishino S,Nomura N,Sako Y,Ishino Y

    更新日期:1999-10-01 00:00:00

  • Conjugation-mediated genetic exchange in Legionella pneumophila.

    abstract::Genetic exchange mechanisms, to our knowledge, have not been reported for Legionella pneumophila, and consequently, studies on the genetic organization of L. pneumophila have not appeared in the literature. Here, we describe gene transfer mediated by broad host range conjugative plasmids in Legionella spp. Escherichia...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.80-84.1985

    authors: Dreyfus LA,Iglewski BH

    更新日期:1985-01-01 00:00:00

  • Effect of Lipidation on the Localization and Activity of a Lysozyme Inhibitor in Neisseria gonorrhoeae.

    abstract::The Gram-negative pathogen Neisseria gonorrhoeae (gonococcus [Gc]) colonizes lysozyme-rich mucosal surfaces. Lysozyme hydrolyzes peptidoglycan, leading to bacterial lysis. Gc expresses two proteins, SliC and NgACP, that bind and inhibit the enzymatic activity of lysozyme. SliC is a surface-exposed lipoprotein, while N...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00633-19

    authors: Ragland SA,Gray MC,Melson EM,Kendall MM,Criss AK

    更新日期:2020-03-26 00:00:00

  • Duplication of a 2,4-dichlorophenoxyacetic acid monooxygenase gene in Alcaligenes eutrophus JMP134(pJP4).

    abstract::The Alcaligenes eutrophus JMP134 plasmid pJP4 contains genes necessary for the complete degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) and 3-chlorobenzoic acid. tfdA encodes 2,4-D monooxygenase, the initial enzyme in the 2,4-D catabolic pathway. The tfdA locus has recently been localized to a region on pJP4 13 ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.12.5669-5672.1988

    authors: Perkins EJ,Lurquin PF

    更新日期:1988-12-01 00:00:00

  • Escherichia coli produces linoleic acid during late stationary phase.

    abstract::Escherichia coli produces linoleic acid in the late stationary phase. This was the case whether the cultures were grown aerobically or anaerobically on a supplemented glucose-salts medium. The linoleic acid was detected by thin-layer chromatography and was measured as the methyl ester by gas chromatography. The linole...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.17.5324-5328.1993

    authors: Rabinowitch HD,Sklan D,Chace DH,Stevens RD,Fridovich I

    更新日期:1993-09-01 00:00:00

  • Role of gene fadR in Escherichia coli acetate metabolism.

    abstract::Mutants of Escherichia coli K-12 constitutive for fatty acid degradation (fadR) showed an increased rate of utilization of exogenous acetate. Acetate transport, oxidation, and incorporation into macromolecules was approximately fivefold greater in fadR mutants than fadR+ strains during growth on succinate as a carbon ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.148.1.83-90.1981

    authors: Maloy SR,Nunn WD

    更新日期:1981-10-01 00:00:00

  • Complete genome sequence of Listeria seeligeri, a nonpathogenic member of the genus Listeria.

    abstract::We report the complete and annotated genome sequence of the nonpathogenic Listeria seeligeri SLCC3954 serovar 1/2b type strain harboring the smallest completely sequenced genome of the genus Listeria. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01415-09

    authors: Steinweg C,Kuenne CT,Billion A,Mraheil MA,Domann E,Ghai R,Barbuddhe SB,Kärst U,Goesmann A,Pühler A,Weisshaar B,Wehland J,Lampidis R,Kreft J,Goebel W,Chakraborty T,Hain T

    更新日期:2010-03-01 00:00:00

  • Increase in transduction efficiency of Tn551 mediated by the methicillin resistance marker.

    abstract::The transduction efficiency of Tn551, a staphylococcal transposon coding for erythromycin resistance (ermB), was increased by a factor of about five in acceptor strains carrying the gene for methicillin resistance (mec), as compared with methicillin-sensitive strains. This enhancement was independent of the generalize...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.1.533-535.1983

    authors: Berger-Bächi B

    更新日期:1983-04-01 00:00:00

  • Structural study of binding of flagellin by Toll-like receptor 5.

    abstract::In order to predict the binding regions within the complex formed by Toll-like receptor 5 (TLR-5) and flagellin, a complementary hydropathy between the two proteins was sought. A region common to the flagellins of Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa, and Listeria monocytogenes was shown to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.14.4243-4247.2003

    authors: Jacchieri SG,Torquato R,Brentani RR

    更新日期:2003-07-01 00:00:00

  • 2-deoxyribose gene-enzyme complex in Salmonella typhimurium. I. Isolation and enzymatic characterization of 2-deoxyribose-negative mutants.

    abstract::Salmonella typhimurium was found to utilize 2-deoxyribose as a sole carbon and energy source. Cells grown in the presence of deoxyribose contained increased levels of deoxyribose kinase, thymidine phosphorylase, and two forms of deoxyribose-5-phosphate aldolase (DR5P aldolase). One form of DR5P aldolase was induced by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.2.449-457.1968

    authors: Hoffee PA

    更新日期:1968-02-01 00:00:00

  • Plasmid-encoded asp operon confers a proton motive metabolic cycle catalyzed by an aspartate-alanine exchange reaction.

    abstract::Tetragenococcus halophila D10 catalyzes the decarboxylation of L-aspartate with nearly stoichiometric release of L-alanine and CO(2). This trait is encoded on a 25-kb plasmid, pD1. We found in this plasmid a putative asp operon consisting of two genes, which we designated aspD and aspT, encoding an L-aspartate-beta-de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.11.2906-2913.2002

    authors: Abe K,Ohnishi F,Yagi K,Nakajima T,Higuchi T,Sano M,Machida M,Sarker RI,Maloney PC

    更新日期:2002-06-01 00:00:00

  • Function of a chemotaxis-like signal transduction pathway in modulating motility, cell clumping, and cell length in the alphaproteobacterium Azospirillum brasilense.

    abstract::A chemotaxis signal transduction pathway (hereafter called Che1) has been previously identified in the alphaproteobacterium Azospirillum brasilense. Previous experiments have demonstrated that although mutants lacking CheB and/or CheR homologs from this pathway are defective in chemotaxis, a mutant in which the entire...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00734-08

    authors: Bible AN,Stephens BB,Ortega DR,Xie Z,Alexandre G

    更新日期:2008-10-01 00:00:00