Abstract:
:The iron-molybdenum cofactor (FeMo-co) of nitrogenase contains molybdenum, iron, sulfur, and homocitrate in a ratio of 1:7:9:1. In vitro synthesis of FeMo-co has been established, and the reaction requires an ATP-regenerating system, dithionite, molybdate, homocitrate, and at least NifB-co (the metabolic product of NifB), NifNE, and dinitrogenase reductase (NifH). The typical in vitro FeMo-co synthesis reaction involves mixing extracts from two different mutant strains of Azotobacter vinelandii defective in the biosynthesis of cofactor or an extract of a mutant strain complemented with the purified missing component. Surprisingly, the in vitro synthesis of FeMo-co with only purified components failed to generate significant FeMo-co, suggesting the requirement for one or more other components. Complementation of these assays with extracts of various mutant strains demonstrated that NifX has a role in synthesis of FeMo-co. In vitro synthesis of FeMo-co with purified components is stimulated approximately threefold by purified NifX. Complementation of these assays with extracts of A. vinelandii DJ42. 48 (DeltanifENX DeltavnfE) results in a 12- to 15-fold stimulation of in vitro FeMo-co synthesis activity. These data also demonstrate that apart from the NifX some other component(s) is required for the cofactor synthesis. The in vitro synthesis of FeMo-co with purified components has allowed the detection, purification, and identification of an additional component(s) required for the synthesis of cofactor.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Shah VK,Rangaraj P,Chatterjee R,Allen RM,Roll JT,Roberts GP,Ludden PWdoi
10.1128/JB.181.9.2797-2801.1999keywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
2797-801issue
9eissn
0021-9193issn
1098-5530journal_volume
181pub_type
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journal_title:Journal of bacteriology
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doi:10.1128/JB.118.3.1067-1074.1974
更新日期:1974-06-01 00:00:00
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更新日期:1981-02-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.133.3.1073-1077.1978
更新日期:1978-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.1.330-333.1984
更新日期:1984-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2009-11-01 00:00:00
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更新日期:1989-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2006-06-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1999-07-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1997-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.141.2.899-907.1980
更新日期:1980-02-01 00:00:00
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更新日期:2007-08-01 00:00:00
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更新日期:2006-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.24.8018-8023.1993
更新日期:1993-12-01 00:00:00
abstract::Seligman, Stephen J. (University of California, Los Angeles) and William L. Hewitt. Kinetics of the action of ampicillin on Escherichia coli. J. Bacteriol. 85:1160-1164. 1963.-The curve of the number of viable Escherichia coli after exposure to ampicillin can be divided into three phases: a lag phase, a rapid bacteric...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.5.1160-1164.1963
更新日期:1963-05-01 00:00:00
abstract::Trent, Dennis W. (University of Oklahoma School of Medicine, Oklahoma City) and L. Vernon Scott. Colorado tick fever virus in cell culture. I. Cell-type susceptibility and interaction with L cells. J. Bacteriol. 88:702-708. 1964.-Colorado tick fever (CTF) virus was serially propagated in monolayer cultures of L and FL...
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doi:10.1128/JB.88.3.702-708.1964
更新日期:1964-09-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1984-10-01 00:00:00
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更新日期:1967-07-01 00:00:00
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更新日期:1962-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.262-268.1982
更新日期:1982-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1976-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.2.286-290.1968
更新日期:1968-02-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1993-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.7.2250-2255.1991
更新日期:1991-04-01 00:00:00
abstract::The effects of competence-inducing treatments on the composition and organization of membrane lipids in Escherichia coli K-12, DH1, DH5, HB101, and RR1 were investigated for two widely used protocols in which transformability is developed at low temperatures in Ca2+ buffers. At stages during each procedure, the lipid ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.553-562.1986
更新日期:1986-11-01 00:00:00