Genetic recombination of bacterial plasmid DNA: effect of RecF pathway mutations on plasmid recombination in Escherichia coli.

Abstract:

:Tn5 insertion mutations in the recN gene, and in what appears to be a new RecF pathway gene designated recO and mapping at approximately 55.4 min on the standard genetic map, were isolated by screening Tn5 insertion mutations that cotransduced with tyrA. The recO1504::Tn5 mutation decreased the frequency of recombination during Hfr-mediated crosses and increased the susceptibility to killing by UV irradiation and mitomycin C when present in a recB recC sbcB background, but only increased the sensitivity to killing by UV irradiation when present in an otherwise Rec+ background. The effects of these and other RecF pathway mutations on plasmid recombination were tested. Mutations in the recJ, recO, and ssb genes, when present in otherwise Rec+ E. coli strains, decreased the frequency of plasmid recombination, whereas the lexA3, recAo281, recN, and ruv mutations had no effect on plasmid recombination. Tn5 insertion mutations in the lexA gene increased the frequency of plasmid recombination. These data indicate that plasmid recombination events in wild-type Escherichia coli strains are catalyzed by a recombination pathway that is related to the RecF recombination pathway and that some component of this pathway besides the recA gene product is regulated by the lexA gene product.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kolodner R,Fishel RA,Howard M

doi

10.1128/JB.163.3.1060-1066.1985

subject

Has Abstract

pub_date

1985-09-01 00:00:00

pages

1060-6

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

163

pub_type

杂志文章
  • Fine structure of sporulation in Bacillus cereus grown in a chemically defined medium.

    abstract::Ellar, D. J. (Syracuse University, Syracuse, N.Y.), and D. G. Lundgren. Fine structure of sporulation in Bacillus cereus grown in a chemically defined medium. J. Bacteriol. 92:1748-1764. 1966.-A study was made of the fine structure of sporulating cells of Bacillus cereus grown in a chemically defined medium. The devel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.6.1748-1764.1966

    authors: Ellar DJ,Lundgren DG

    更新日期:1966-12-01 00:00:00

  • nodO, a new nod gene of the Rhizobium leguminosarum biovar viciae sym plasmid pRL1JI, encodes a secreted protein.

    abstract::The region of the Rhizobium leguminosarum biovar viciae Sym plasmid pRL1JI, responsible for the production and secretion of a previously described 50-kilodalton protein (R. A. de Maagd, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg, J. Bacteriol. 170:4424-4427, 1988), was cloned and its nucleotide sequence was de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.12.6764-6770.1989

    authors: de Maagd RA,Wijfjes AH,Spaink HP,Ruiz-Sainz JE,Wijffelman CA,Okker RJ,Lugtenberg BJ

    更新日期:1989-12-01 00:00:00

  • Impact of phosphorylation of specific residues in the tyrosine autokinase, Wzc, on its activity in assembly of group 1 capsules in Escherichia coli.

    abstract::Wzc(CPS) is a tyrosine autokinase essential for the assembly of a high-molecular-weight (HMW) group 1 capsular polysaccharide (CPS) in Escherichia coli. Homologues of Wzc participate in the formation of CPS and exopolysaccharides in a variety of gram-positive and gram-negative bacteria. Phosphorylation of tyrosine res...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.23.6437-6447.2002

    authors: Paiment A,Hocking J,Whitfield C

    更新日期:2002-12-01 00:00:00

  • Alcaligenes eutrophus JMP134 "2,4-dichlorophenoxyacetate monooxygenase" is an alpha-ketoglutarate-dependent dioxygenase.

    abstract::The Alcaligenes eutrophus JMP134 tfdA gene, encoding the enzyme responsible for the first step in 2,4-dichlorophenoxyacetic acid (2,4-D) biodegradation, was overexpressed in Escherichia coli, and several enzymatic properties of the partially purified gene product were examined. Although the tfdA-encoded enzyme is typi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.7.2083-2086.1993

    authors: Fukumori F,Hausinger RP

    更新日期:1993-04-01 00:00:00

  • Analysis of storage lipid accumulation in Alcanivorax borkumensis: Evidence for alternative triacylglycerol biosynthesis routes in bacteria.

    abstract::Marine hydrocarbonoclastic bacteria, like Alcanivorax borkumensis, play a globally important role in bioremediation of petroleum oil contamination in marine ecosystems. Accumulation of storage lipids, serving as endogenous carbon and energy sources during starvation periods, might be a potential adaptation mechanism f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01292-06

    authors: Kalscheuer R,Stöveken T,Malkus U,Reichelt R,Golyshin PN,Sabirova JS,Ferrer M,Timmis KN,Steinbüchel A

    更新日期:2007-02-01 00:00:00

  • Genomic analysis of Rickettsia japonica strain YHT.

    abstract::Rickettsia japonica strain YH, isolated in 1984 in Japan, is the type strain of R. japonica, the tick-borne agent of Japanese spotted fever. Here, we report the 1.33-Mb genome of this rickettsial species. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01928-12

    authors: Dong X,El Karkouri K,Robert C,Raoult D,Fournier PE

    更新日期:2012-12-01 00:00:00

  • DnaA protein is required for replication of the minimal replicon of the broad-host-range plasmid RK2 in Escherichia coli.

    abstract::The minimal origin of replication of the broad-host-range plasmid RK2 has two potential recognition sequences for the DnaA protein of Escherichia coli. DNA transfer by transformation into a dnaA-null mutant of E. coli showed that DnaA protein is needed for replication or maintenance of mini-RK2. We isolated and purifi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.10.4703-4709.1987

    authors: Gaylo PJ,Turjman N,Bastia D

    更新日期:1987-10-01 00:00:00

  • Transport of biosynthetic intermediates: homoserine and threonine uptake in Escherichia coli.

    abstract::Although amino acid transport has been extensively studied in bacteria during the past decade, little is known concerning the transport of those amino acids that are biosynthetic intermediates or have multiple fates within the cell. We have studied homoserine and threonine as examples of this phenomenon. Homoserine is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.3.1002-1009.1974

    authors: Templeton BA,Savageau MA

    更新日期:1974-03-01 00:00:00

  • Complementation of the lytD1 mutation of Escherichia coli by either the cI or cro gene of bacteriophage lambda.

    abstract::The lytD1 mutant of Escherichia coli exhibits temperature-sensitive growth which is attributed to cellular autolysis at the restrictive temperature. Either of two cloned phage lambda genes, identified as cI and cro, suppressed the lytD1(Ts) lysis phenotype, suggesting that lytD encodes a DNA-binding protein with a DNA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.2.893-895.1991

    authors: Dai DX,Ishiguro EE

    更新日期:1991-01-01 00:00:00

  • Succinic Semialdehyde Promotes Prosurvival Capability of Agrobacterium tumefaciens.

    abstract:UNLABELLED:Succinic semialdehyde (SSA), an important metabolite of γ-aminobutyric acid (GABA), is a ligand of the repressor AttJ regulating the expression of the attJ-attKLM gene cluster in the plant pathogen Agrobacterium tumefaciens. While the response of A. tumefaciens to GABA and the function of attKLM have been ex...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00373-15

    authors: Wang C,Tang D,Gao YG,Zhang LH

    更新日期:2016-01-11 00:00:00

  • Relation of catalase to substrate utilization by Mycoplasma pneumoniae.

    abstract::No catalase activity was detected in four strains of glucose-grown Mycoplasma pneumoniae at any time during the replication of the organism. Exogenous catalase dramatically increased the O(2) uptake with glycerol, presumably by releasing inhibition caused by hydrogen peroxide. The effect of added catalase on the O(2) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.4.1425-1430.1968

    authors: Low IE,Eaton MD,Proctor P

    更新日期:1968-04-01 00:00:00

  • Involvement of carbon source and acetyl phosphate in the external-pH-dependent expression of porin genes in Escherichia coli.

    abstract::The porin composition of the Escherichia coli cell envelope was analyzed during growth at different external pHs (pHo) as a function of the acetyl phosphate (AcP) level (DeltaackA pta or ackA mutant, pyruvate or glucose as the carbon source) in the presence or absence of EnvZ. Our results indicate that the AcP level i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.1.198-202.2000

    authors: Heyde M,Laloi P,Portalier R

    更新日期:2000-01-01 00:00:00

  • Ascospore wall development in Saccharomyces cerevisiae.

    abstract::Ascospore delimitation in Saccharomyces cerevisiae is initiated by a pair of unit membranes between which the spore wall is subsequently laid down. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.2.1054-1057.1973

    authors: Beckett A,Illingworth RF,Rose AH

    更新日期:1973-02-01 00:00:00

  • Ethylmalonyl coenzyme A mutase operates as a metabolic control point in Methylobacterium extorquens AM1.

    abstract::The metabolism of one- and two-carbon compounds by the methylotrophic bacterium Methylobacterium extorquens AM1 involves high carbon flux through the ethylmalonyl coenzyme A (ethylmalonyl-CoA) pathway (EMC pathway). During growth on ethylamine, the EMC pathway operates as a linear pathway carrying the full assimilator...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02478-14

    authors: Good NM,Martinez-Gomez NC,Beck DA,Lidstrom ME

    更新日期:2015-02-15 00:00:00

  • Pediococcus cerevisiae mutant with altered transport of folates.

    abstract::A Pediococcus cerevisiae mutant that actively accumulated folate (PteGlu), in contrast to the wild-type, was also found to exhibit changes in the pattern of uptake of 5-methyl-tetrahydrofolate (5-CH3-H4PteGlu) and amethopterin. Most of the 5-CH3-H4PteGlue accumulated through a glucose- and temperature-dependent proces...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.2.400-406.1975

    authors: Mandelbaum-Shavit F,Grossowicz N

    更新日期:1975-08-01 00:00:00

  • RNA-Mediated cis Regulation in Acinetobacter baumannii Modulates Stress-Induced Phenotypic Variation.

    abstract::In the nosocomial opportunistic pathogen Acinetobacter baumannii, RecA-dependent mutagenesis, which causes antibiotic resistance acquisition, is linked to the DNA damage response (DDR). Notably, unlike the Escherichia coli paradigm, recA and DDR gene expression in A. baumannii is bimodal. Namely, there is phenotypic v...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00799-16

    authors: Ching C,Gozzi K,Heinemann B,Chai Y,Godoy VG

    更新日期:2017-05-09 00:00:00

  • Growth phase-coupled changes of the ribosome profile in natural isolates and laboratory strains of Escherichia coli.

    abstract::The growth phase-dependent change in sucrose density gradient centrifugation patterns of ribosomes was analyzed for both laboratory strains of Escherichia coli and natural isolates from the ECOR collection. All of the natural isolates examined formed 100S ribosome dimers in the stationary phase, and ribosome modulatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.10.2893-2899.2000

    authors: Wada A,Mikkola R,Kurland CG,Ishihama A

    更新日期:2000-05-01 00:00:00

  • New Twists and Turns in Bacterial Locomotion and Signal Transduction.

    abstract::Prokaryotic organisms occupy the most diverse set of environments and conditions on our planet. Their ability to sense and respond to a broad range of external cues remain key research areas in modern microbiology, central to behaviors that underlie beneficial and pathogenic interactions of bacteria with multicellular...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00439-19

    authors: Watts KJ,Vaknin A,Fuqua C,Kazmierczak BI

    更新日期:2019-09-20 00:00:00

  • Protonmotive force and bacterial sensing.

    abstract::The role of the proton gradient and external pH in the motility and chemotaxis of Bacillus subtilis was investigated. Presence of a substantial proton gradient is not necessary for motility or chemotaxis, as long as the electrical potential is sufficient to maintain motility. Changes in the proton gradient do, however...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.1.26-32.1980

    authors: Miller JB,Koshland DE Jr

    更新日期:1980-01-01 00:00:00

  • Regulation of allantoate transport in wild-type and mutant strains of Saccharomyces cerevisiae.

    abstract::Accumulation of intracellular allantoin and allantoate is mediated by two distinct active transport systems in Saccharomyces cerevisiae. Allantoin transport (DAL4 gene) is inducible, while allantoate uptake is constitutive (it occurs at full levels in the absence of any allantoate-related compounds from the culture me...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.4.1684-1690.1987

    authors: Chisholm VT,Lea HZ,Rai R,Cooper TG

    更新日期:1987-04-01 00:00:00

  • Magnesium starvation of Aerobacter aerogenes. II. Rates of nucleic acid synthesis and methods for their measurement.

    abstract::The rates of synthesis of Aerobacter aerogenes nucleic acids were estimated during incubation of the bacteria in a Mg(++)-free medium. Deoxyribonucleic acid (DNA) synthesized during Mg(++) starvation, or in the preceding exponential growth, remained acid-precipitable for 2.5 hr before breaking down to acid-soluble pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.1.345-356.1967

    authors: Kennell D,Kotoulas A

    更新日期:1967-01-01 00:00:00

  • Physical aggregation and functional reconstitution of solubilized membranes of Bacillus stearothermophilus.

    abstract::Isolated membranes of Bacillus stearothermophilus 2184D can be disrupted by treatment with sodium dodecyl sulfate (SDS). This disruption is attended by a decreased turbidity of membrane suspensions and a differential loss of activities of the electron transport system. Reduced methyl viologen (MVH)-nitrate reductase a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.2.811-819.1972

    authors: Kiszkiss DF,Downey RJ

    更新日期:1972-02-01 00:00:00

  • Location of bacterial polysaccharide during various phases of growth.

    abstract::Polysaccharide-containing material was located by histochemical staining on ultrathin sections of Bacillus cereus and several clostridial species during various phases of growth. These components were located along the cell wall in young vegetative cells and along the developing cortex in sporulating cells. In B. cere...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.3.1342-1354.1969

    authors: Walker PD,Short J

    更新日期:1969-06-01 00:00:00

  • NUTRITIONAL MUTATIONS AND TRANSDUCTION BY ULTRAVIOLET-INACTIVATED PHAGE IN STAPHYLOCOCCUS AUREUS.

    abstract::Carere, A., and Isabella Spada-Sermonti (Istituto Superiore di Sanità, Rome, Italy). Nutritional mutations and transduction by ultraviolet-inactivated phage in Staphylococcus aureus. J. Bacteriol. 88:226-232. 1964.-By use of a semisynthetic medium, various nutritional mutants of Staphylococcus aureus 80 were isolated....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.1.226-232.1964

    authors: CARERE A,SPADA-SERMONTI I

    更新日期:1964-07-01 00:00:00

  • Electron microscopy of Staphylococcus aureus cells and cell walls after treatment with lysozyme Chalaropsis.

    abstract::Lysozyme Chalaropsis, a beta-1,4-N,6-O-diacetylmuramidase, attacks the cell wall murein of Staphylococcus aureus H in a random manner. In hypertonic sucrose solutions, true protoplasts are produced and, in the process, mesosomal tubules are extruded into the medium. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.2.900-903.1974

    authors: Robinson JP,Robinson RD,Hash JH

    更新日期:1974-02-01 00:00:00

  • YjbH Requires Its Thioredoxin Active Motif for the Nitrosative Stress Response, Cell-to-Cell Spread, and Protein-Protein Interactions in Listeria monocytogenes.

    abstract::Listeria monocytogenes is a model facultative intracellular pathogen. Tight regulation of virulence proteins is essential for a successful infection, and the gene encoding the annotated thioredoxin YjbH was identified in two forward genetic screens as required for virulence factor production. Accordingly, an L. monocy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00099-20

    authors: Ruhland BR,Reniere ML

    更新日期:2020-05-27 00:00:00

  • Mapping of a structural gene for valyl-transfer ribonucleic acid synthetase in Escherichia coli by transduction.

    abstract::A structural gene, valS, for the valyl-transfer ribonucleic acid synthetase of Escherichia coli has been mapped on the clockwise side of pyrB and is closely linked to it. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.2.837-839.1969

    authors: Tingle MA,Neidhardt FC

    更新日期:1969-05-01 00:00:00

  • Heat reactivation of the alpha-hemolytic, dermonecrotic, lethal activities of crude and purified staphylococcal alpha-toxin.

    abstract::Manohar, M. (University of Minnesota, St. Paul), S. Kumar, and R. K. Lindorfer. Heat reactivation of the alpha-hemolytic, dermonecrotic, and lethal activities of crude and purified staphylococcal alpha-toxin. J. Bacteriol. 91:1681-1685. 1966.-Crude staphylococcal toxin loses its alpha-hemolytic activity more rapidly a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.5.1681-1685.1966

    authors: Manohar M,Kumar S,Lindorfer RK

    更新日期:1966-05-01 00:00:00

  • The herpes simplex virus thymidine kinase gene is not transcribed in Saccharomyces cerevisiae.

    abstract::The herpes simplex virus thymidine kinase gene has been cloned into a chimeric yeast plasmid cloning vehicle and transformed into appropriate yeast strains. Plasmids carrying the herpes simplex virus thymidine kinase gene can be propagated as autonomously replicating plasmids, but no RNA specific to the thymidine kina...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.2.542-547.1982

    authors: Kiss GB,Pearlman RE,Cornish KV,Friesen JD,Chan VL

    更新日期:1982-02-01 00:00:00

  • Regulatory elements of the raffinose operon: nucleotide sequences of operator and repressor genes.

    abstract::The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor (rafR gene) to raf operator (rafO) DNA. Both rafR and rafO have been sequenced. The 1,011-base-pair rafR gene encodes a 336-amino-acid polypeptide containing an N-terminal helix-turn-helix motif. rafO, as defined by in vivo ti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.4.2178-2180.1990

    authors: Aslanidis C,Schmitt R

    更新日期:1990-04-01 00:00:00