Evidence for the existence of two arginyl-transfer ribonucleic acid synthetase activities in Escherichia coli.

abstract：:Many surface proteins of pathogenic gram-positive bacteria are linked to the cell wall envelope by a mechanism requiring a C-terminal sorting signal with an LPXTG motif. Surface proteins of Streptococcus pneumoniae harbor another motif, YSIRK-G/S, which is positioned within signal peptides. The signal peptides of some...

journal_title：Journal of bacteriology

authors： Bae T,Schneewind O

更新日期：2003-05-01 00:00:00

abstract：:The deoxyribonucleic acid base composition (percent guanine + cytosine [GC]) was determined for 29 strains, representing 18 species of the genus Kluyveromyces. It was concluded that on the basis of GC content (47.4%) and other properties K. veronae occupies an uncertain position in the genus Kluyveromyces. The GC cont...

journal_title：Journal of bacteriology

authors： Martini A,Phaff HJ,Douglass SA

更新日期：1972-08-01 00:00:00

abstract：:The expression of Escherichia coli purR, which encodes the pur regulon repressor protein, is autoregulated. Autoregulation at the level of transcription requires two operator sites, designated purRo1 and purRo2 (O1 and O2). Operator O1 is in the region of DNA between the transcription start site and the site for trans...

journal_title：Journal of bacteriology

authors： Rolfes RJ,Zalkin H

更新日期：1990-10-01 00:00:00

abstract：:During nitrogen-limited growth, transcription of glnA, which codes for glutamine synthetase, requires sigma 54-RNA polymerase and the phosphorylated from the nitrogen regulator I (NRI; also called NtrC). In cells in which the lac promoter controlled expression of the gene coding for NRI, increasing the intracellular c...

journal_title：Journal of bacteriology

authors： Shiau SP,Schneider BL,Gu W,Reitzer LJ

更新日期：1992-01-01 00:00:00

abstract：:Pulse-labeled ribonucleic acid (RNA) was extracted from polysomes of sporulating cells of Saccharomyces cerevisiae and characterized in sucrose gradients and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Transfer RNA, ribosomal RNA, and heterodisperse RNA, presumed to be messenger RNA, were synthesized...

journal_title：Journal of bacteriology

authors： Curiale MS,Petryna MM,Mills D

更新日期：1976-05-01 00:00:00

abstract：:The lytD1 mutant of Escherichia coli exhibits temperature-sensitive growth which is attributed to cellular autolysis at the restrictive temperature. Either of two cloned phage lambda genes, identified as cI and cro, suppressed the lytD1(Ts) lysis phenotype, suggesting that lytD encodes a DNA-binding protein with a DNA...

journal_title：Journal of bacteriology

authors： Dai DX,Ishiguro EE

更新日期：1991-01-01 00:00:00

abstract：:A mutant of Mycobacterium smegmatis unable to use the dipeptide carnosine (beta-alanyl-L-histidine) as a sole carbon or nitrogen source was isolated. Carnosinase activity and the ability to grow on beta-Ala and/or L-His were similar in the mutant and the wild type. However, the mutant showed significant impairment in ...

journal_title：Journal of bacteriology

authors： Bhatt A,Green R,Coles R,Condon M,Connell ND

更新日期：1998-12-01 00:00:00

abstract：:Incubation of ultraviolet-irradiated Escherichia coli B/r cultures with 0.7% Triton X-100 resulted in a large decrease in turbidity. Under phase-contrast optics, most of the irradiated detergent-treated cells were smaller than normal and of low phase density; only a small percentage were normal or larger than normal a...

journal_title：Journal of bacteriology

authors： Schenley RL,Fisher WD,Swenson PA,Khachatourians GG

更新日期：1976-05-01 00:00:00

abstract：:Adenylate cyclases (ACs) catalyze the formation of 3',5'-cyclic AMP (cAMP) from ATP. A novel AC-encoding gene, cyaC, was isolated from Rhizobium etli by phenotypic complementation of an Escherichia coli cya mutant. The functionality of the cyaC gene was corroborated by its ability to restore cAMP accumulation in an E....

journal_title：Journal of bacteriology

authors： Téllez-Sosa J,Soberón N,Vega-Segura A,Torres-Márquez ME,Cevallos MA

更新日期：2002-07-01 00:00:00

abstract：:We have developed a rapid and sensitive fluorimetric method, based on the formation of a fluorescent product from nitrosation of 2,3-diaminonaphthalene, for measuring the ability of bacteria to catalyze nitrosation of amines. We have shown in Escherichia coli that nitrosation can be induced under anaerobic conditions ...

journal_title：Journal of bacteriology

authors： Ralt D,Wishnok JS,Fitts R,Tannenbaum SR

更新日期：1988-01-01 00:00:00

abstract：:Restriction fragments containing the gene encoding cyanase, cynS, without its transcriptional regulatory sequences were placed downstream of lac and tac promoters in various pUC derivatives to maximize production of cyanase. Plasmid pSJ105, which contains the cynS gene and an upstream open reading frame, gave the high...

journal_title：Journal of bacteriology

authors： Sung YC,Anderson PM,Fuchs JA

更新日期：1987-11-01 00:00:00

abstract：:Three independently isolated metK mutants have been shown to have leisions lying between speB and glc near 57 min on the Escherichia coli chromosome. Two deletions result in a lack of the metC gene product but neither extends into the metK glc region. The three metK mutations are recessive to the wild-type allele carr...

journal_title：Journal of bacteriology

authors： Hunter JS,Greene RC,Su CH

更新日期：1975-06-01 00:00:00

abstract：:We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mit...

journal_title：Journal of bacteriology

authors： Jefferies J,Nieminen L,Kirkham LA,Johnston C,Smith A,Mitchell TJ

更新日期：2007-01-01 00:00:00

abstract：:Cystic fibrosis transmembrane conductance regulator (CFTR) inhibitory factor (Cif) is a virulence factor secreted by Pseudomonas aeruginosa that reduces the quantity of CFTR in the apical membrane of human airway epithelial cells. Initial sequence analysis suggested that Cif is an epoxide hydrolase (EH), but its seque...

journal_title：Journal of bacteriology

authors： Bahl CD,Morisseau C,Bomberger JM,Stanton BA,Hammock BD,O'Toole GA,Madden DR

更新日期：2010-04-01 00:00:00

abstract：:Polynucleotide phosphorylase (PNP) plays a central role in RNA degradation, generating a pool of ribonucleoside diphosphates (rNDPs) that can be converted to deoxyribonucleoside diphosphates (dNDPs) by ribonucleotide reductase. We report here that spontaneous mutations resulting from replication errors, which are norm...

journal_title：Journal of bacteriology

authors： Becket E,Tse L,Yung M,Cosico A,Miller JH

更新日期：2012-10-01 00:00:00

abstract：:In order to examine the potential role of bacterial collagenases in periodontal tissue destruction, we recently isolated a gene, prtC, from Porphyromonas gingivalis ATCC 53977, which expressed collagenase activity (N. Takahashi, T. Kato, and H. K. Kuramitsu, FEMS Microbiol. Lett. 84:135-138, 1991). The nucleotide sequ...

journal_title：Journal of bacteriology

authors： Kato T,Takahashi N,Kuramitsu HK

更新日期：1992-06-01 00:00:00

abstract：:Legionella pneumophila is the causative agent of the severe and potentially fatal pneumonia Legionnaires' disease. L. pneumophila is able to replicate within macrophages and protozoa by establishing a replicative compartment in a process that requires the Icm/Dot type IVB secretion system. The signals and regulatory p...

journal_title：Journal of bacteriology

authors： Hovel-Miner G,Pampou S,Faucher SP,Clarke M,Morozova I,Morozov P,Russo JJ,Shuman HA,Kalachikov S

更新日期：2009-04-01 00:00:00

abstract：:The mobile element IS256 causes phase variation of biofilm formation in Staphylococcus epidermidis by insertion and precise excision from the icaADBC operon. Precise excision, i.e., removal of the target site duplications (TSDs) and restoration of the original DNA sequence, occurs rarely but independently of functiona...

journal_title：Journal of bacteriology

authors： Hennig S,Ziebuhr W

更新日期：2008-02-01 00:00:00

abstract：:The proteome of Haemophilus influenzae strain Rd KW20 was analyzed by liquid chromatography (LC) coupled with ion trap tandem mass spectrometry (MS/MS). This approach does not require a gel electrophoresis step and provides a rapidly developed snapshot of the proteome. In order to gain insight into the central metabol...

journal_title：Journal of bacteriology

authors： Kolker E,Purvine S,Galperin MY,Stolyar S,Goodlett DR,Nesvizhskii AI,Keller A,Xie T,Eng JK,Yi E,Hood L,Picone AF,Cherny T,Tjaden BC,Siegel AF,Reilly TJ,Makarova KS,Palsson BO,Smith AL

更新日期：2003-08-01 00:00:00

abstract：:Analysis of the deoxyribonucleic acid prepared from Proteus mirabilis, Escherichia coli, and Serratia marcescens in an alkaline CsCl gradient has shown that there is an asymmetric distribution of guanine plus thymine residues between the complementary strands of the deoxyribonucleic acid. ...

journal_title：Journal of bacteriology

authors： Hershberger C,Mickel S,Rownd R

更新日期：1971-04-01 00:00:00

abstract：:Rhodobacter capsulatus mutants unable to fix nitrogen were isolated by random transposon Tn5 mutagenesis. The Tn5 insertion sites of 30 Nif- mutants were mapped within three unlinked chromosomal regions designated A, B, and C. The majority of Tn5 insertions (21 mutants) map within nif region A, characterized by two Cl...

journal_title：Journal of bacteriology

authors： Klipp W,Masepohl B,Pühler A

更新日期：1988-02-01 00:00:00

abstract：:Pseudomonas sp. isolated by selective culture with 3-phenylbutyrate (3-PB) as the sole carbon source metabolized the compound through two different pathways by initial oxidation of the benzene ring and by initial oxidation of the side chain. During early exponential growth, a catechol substance identified as 3-(2,3-di...

journal_title：Journal of bacteriology

authors： Sariaslani FS,Sudmeier JL,Focht DD

更新日期：1982-10-01 00:00:00

abstract：:Certain carbohydrates (rhamnose, 3-O-methyl rhamnose, and galactosamine) have been demonstrated to be present in Bacillus anthracis spores but absent in vegetative cells. Others have demonstrated that these spore-specific sugars are constituents of the glycoprotein BclA. In the current work, spore extracts were separa...

journal_title：Journal of bacteriology

authors： Waller LN,Stump MJ,Fox KF,Harley WM,Fox A,Stewart GC,Shahgholi M

更新日期：2005-07-01 00:00:00

abstract：:A 7.5-kbp fragment of chromosomal DNA downstream of the Vibrio cholerae vibriobactin outer membrane receptor, viuA, and the vibriobactin utilization gene, viuB, was recovered from a Sau3A lambda library of O395 chromosomal DNA. By analogy with the genetic organization of the Escherichia coli enterobactin gene cluster,...

journal_title：Journal of bacteriology

authors： Butterton JR,Choi MH,Watnick PI,Carroll PA,Calderwood SB

更新日期：2000-03-01 00:00:00

abstract：:A gene which complements a paralyzed flagellar mutant of Rhodobacter sphaeroides was sequenced. The derived protein sequence has similarity to MotB. R. sphaeroides MotB lacks the C-terminal peptidoglycan-binding motif of other MotB proteins. This divergence of sequence may reflect the unusual, unidirectional, stop-sta...

journal_title：Journal of bacteriology

authors： Shah DS,Armitage JP,Sockett RE

更新日期：1995-05-01 00:00:00

abstract：:An in vitro transposition system, developed to facilitate gene disruption in Deinococcus radiodurans R1, has been used to inactivate the gene designated dr1819 in uvrA-1(+) and uvrA-1 backgrounds. dr1819 encodes a protein with homology to a UV DNA damage endonuclease expressed by Schizosaccharomyces pombe. Interruptio...

journal_title：Journal of bacteriology

authors： Earl AM,Rankin SK,Kim KP,Lamendola ON,Battista JR

更新日期：2002-02-01 00:00:00

abstract：:Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, a serious disease of rice. Spontaneous mutants which are deficient for virulence and extracellular polysaccharide (Eps) production accumulate in large numbers in stationary-phase cultures of this bacterium, a phenomenon which we have called stationary-phase v...

journal_title：Journal of bacteriology

authors： Rajeshwari R,Sonti RV

更新日期：2000-09-01 00:00:00

abstract：:Transducing frequencies of phage P22 lysates prepared from Salmonella typhimurium exponential cultures in minimal and nutrient broth media were compared. The assumption is that cells grown in a minimal medium will have one replication fork per replication unit, but cells in nutrient broth will have multiple replicatio...

journal_title：Journal of bacteriology

authors： Fujisawa T,Eisenstark A

更新日期：1973-07-01 00:00:00

abstract：:The colicin E2 immunity (ceiB) and lysis (celB) genes of colicin plasmid ColE2-P9 were cloned as a 900-base-pair insert under the control of the lac promoter in high-copy-number plasmid pUR222. Hosts carrying this plasmid were immune to colicin E2, produced increased amounts of immunity protein (molecular weight, 9,00...

journal_title：Journal of bacteriology

authors： Pugsley AP,Schwartz M

更新日期：1983-10-01 00:00:00

abstract：:In studies of Pseudomonas putida IH-2000, a toluene-tolerant microorganism, membrane vesicles (MVs) were found to be released from the outer membrane when toluene was added to the culture. These MVs were found to be composed of phospholipids, lipopolysaccharides (LPS), and very low amounts of outer membrane proteins. ...

journal_title：Journal of bacteriology

authors： Kobayashi H,Uematsu K,Hirayama H,Horikoshi K

更新日期：2000-11-01 00:00:00