Abstract:
:Hydrogenotrophic methanogenic Archaea are defined by an H2 requirement for growth. Despite this requirement, many hydrogenotrophs are also capable of growth with formate as an electron donor for methanogenesis. While certain responses of these organisms to hydrogen availability have been characterized, responses to formate starvation have not been reported. Here we report that during continuous culture of Methanococcus maripaludis under defined nutrient conditions, growth yields relative to methane production decreased markedly with either H2 excess or formate excess. Analysis of the growth yields of several mutants suggests that this phenomenon occurs independently of the storage of intracellular carbon or a transcriptional response to methanogenesis. Using microarray analysis, we found that the expression of genes encoding coenzyme F420-dependent steps of methanogenesis, including one of two formate dehydrogenases, increased with H2 starvation but with formate occurred at high levels regardless of limitation or excess. One gene, encoding H2-dependent methylene-tetrahydromethanopterin dehydrogenase, decreased in expression with either H2 limitation or formate limitation. Expression of genes for the second formate dehydrogenase, molybdenum-dependent formylmethanofuran dehydrogenase, and molybdenum transport increased specifically with formate limitation. Of the two formate dehydrogenases, only the first could support growth on formate in batch culture where formate was in excess.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Costa KC,Yoon SH,Pan M,Burn JA,Baliga NS,Leigh JAdoi
10.1128/JB.02141-12subject
Has Abstractpub_date
2013-04-01 00:00:00pages
1456-62issue
7eissn
0021-9193issn
1098-5530pii
JB.02141-12journal_volume
195pub_type
杂志文章abstract::Mutans of Pseudomonas testosteroni were isolated for their inability to grow on m-hydroxybenzoate as sole carbon source. These mutants hydroxylated m-hydroxybenzoate for form 2,3-dihydroxybenzoate in high yeilds. The bioconversion described in this report represents the first reported example of 3-hydroxybenzoate 2-hy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.1.293-296.1980
更新日期:1980-01-01 00:00:00
abstract::Archaea, constituting the third primary domain of life, harbor a basal transcription apparatus of the eukaryotic type, whereas curiously, a large fraction of the potential transcription regulation factors appear to be of the bacterial type. To date, little information is available on these predicted regulators and on ...
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journal_title:Journal of bacteriology
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doi:10.1128/jb.186.7.2221-2223.2003
更新日期:2004-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.15.4877-4888.1991
更新日期:1991-08-01 00:00:00
abstract::The kil-kor regulon of the self-transmissible, broad-host-range plasmid RK2 is a unique network with eight coregulated operons. Among the genes encoded by the kil-kor regulon are trfA, which encodes the replication initiator, and several kil loci (kilA, kilB, kilC, and kilE), each of which is lethal to the host cell i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.8.2423-2435.1993
更新日期:1993-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-10-01 00:00:00
abstract::The Saccharomyces cerevisiae HIS7 gene was cloned by its location immediately downstream of the previously isolated and characterized ARO4 gene. The two genes have the same orientation with a distance of only 416 bp between the two open reading frames. The yeast HIS7 gene represents the first isolated eukaryotic gene ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.17.5548-5558.1993
更新日期:1993-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.24.7918-7930.1993
更新日期:1993-12-01 00:00:00
abstract::The regrowth of 27 temperature-sensitive division mutants of Streptococcus faecium ATCC 9790 was examined after various periods of incubation at the nonpermissive temperature. Several of the mutants blocked at various stages of septum formation or of daughter-cell separation divided in a partially or completely synchr...
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pub_type: 杂志文章
doi:10.1128/JB.160.1.427-429.1984
更新日期:1984-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.3.725-730.1970
更新日期:1970-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.3.578-585.1998
更新日期:1998-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.1.45-54.2006
更新日期:2006-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.2.416-421.1975
更新日期:1975-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.19.5841-5843.1996
更新日期:1996-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.3.818-823.1986
更新日期:1986-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01050-13
更新日期:2014-01-01 00:00:00
abstract::The ubiquitous opportunistic pathogen Pseudomonas aeruginosa has five aerobic terminal oxidases: bo(3)-type quinol oxidase (Cyo), cyanide-insensitive oxidase (CIO), aa3-type cytochrome c oxidase (aa3), and two cbb(3)-type cytochrome c oxidases (cbb(3)-1and cbb(3)-2). These terminal oxidases are differentially regulate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2014-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00742-10
更新日期:2010-11-01 00:00:00
abstract::A combined physical and genetic map of the Serpulina hyodysenteriae B78T genome was constructed by using pulsed-field gel electrophoresis and DNA blot hybridizations. The S. hyodysenteriae genome is a single circular chromosome about 3.2 Mb in size. The physical map of the chromosome was constructed with the restricti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.4.1087-1092.1994
更新日期:1994-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.3.1424-1427.1980
更新日期:1980-03-01 00:00:00
abstract::The lipopolysaccharide (LPS) from Rhizobium trifolii 0403 was isolated at different stages of growth and was examined for its (i) ability to bind a white clover lectin (trifoliin A), (ii) immunochemical properties, and (iii) composition. There was significantly more binding of trifoliin A to purified LPS and cells in ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.148.2.697-711.1981
更新日期:1981-11-01 00:00:00
abstract::Feldman, L. A. (Baylor University College of Medicine, Houston, Tex.), J. L. Melnick, and F. Rapp. Influence of SV40 genome on the replication of an adenovirus-SV40 "hybrid" population. J. Bacteriol. 90:778-782. 1965.-Replication of a type 7 adenovirus-SV40 hybrid population in primary African green monkey kidney cell...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.3.778-782.1965
更新日期:1965-09-01 00:00:00
abstract:UNLABELLED:Bacterial type IV coupling proteins (T4CPs) bind and mediate the delivery of DNA substrates through associated type IV secretion systems (T4SSs). T4CPs consist of a transmembrane domain, a conserved nucleotide-binding domain (NBD), and a sequence-variable helical bundle called the all-alpha domain (AAD). In ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00189-15
更新日期:2015-07-01 00:00:00
abstract::Enzymological studies were done to characterize the allosteric control of 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase in three species of Clostridium. Allosteric control was identified as feedback inhibition by phenylalanine and was qualitatively similar for the DAHP synthetases of C. butyricum, C. a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.3.641-648.1972
更新日期:1972-09-01 00:00:00
abstract::Rhizobia form a symbiotic relationship with plants of the legume family to produce nitrogen-fixing root nodules under nitrogen-limiting conditions. We have examined the importance of glutathione (GSH) during free-living growth and symbiosis of Sinorhizobium meliloti. An S. meliloti mutant strain (SmgshA) which is unab...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.1.168-174.2005
更新日期:2005-01-01 00:00:00
abstract::The translation inhibitor microcin C7 (MccC7) is a linear heptapeptide whose N terminus has been replaced by an N-formyl group and whose C terminus has been replaced by the phosphodiester of 5'-adenylic acid and n-aminopropanol (J. I. Guijarro, J. E. González-Pastor, F. Baleux, J. L. San Millán, M. A. Castilla, M. Ric...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.24.7131-7140.1995
更新日期:1995-12-01 00:00:00
abstract::Mycelial mats of Ascodesmis sphaerospora were fixed and embedded for electron microscopy, and thin sections of 1-mm blocks, taken from the 1st to the 7th mm behind the hyphal tips, were cut parallel to the long axis of the hyphae. The hyphal tip region is characterized by an outer zone of electron-transparent vesicles...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.2.658-671.1968
更新日期:1968-02-01 00:00:00
abstract::The KRE1 gene of Saccharomyces cerevisiae, sacKRE1, appears to be involved in the synthesis of cell wall beta-glucan. S. cerevisiae strains with mutations in the KRE1 gene produce a structurally altered cell wall (1----6)-beta-glucan, which results in resistance to K1 killer toxin. We isolated the canKRE1 gene from Ca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.21.6859-6864.1991
更新日期:1991-11-01 00:00:00
abstract::When Azotobacter vinelandii was derepressed for nitrogenase synthesis in a N-free medium containing tungstate instead of molybdate, an inactive component I was synthesized. Although this inactive component I could be activated in vivo upon addition of molybdate to the medium, it could not be activated in vitro when mo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.2.697-701.1974
更新日期:1974-11-01 00:00:00