Role of the novel OprD family of porins in nutrient uptake in Pseudomonas aeruginosa.

abstract：:Clinical isolates of the porcine pathogen Actinobacillus pleuropneumoniae often form adherent colonies on agar plates due to expression of an operon, pgaABCD, encoding a poly-beta-1,6-N-acetyl-D-glucosamine (PGA) extracellular matrix. The adherent colony phenotype, which correlates with the ability to form biofilms on...

journal_title：Journal of bacteriology

authors： Bossé JT,Sinha S,Li MS,O'Dwyer CA,Nash JH,Rycroft AN,Kroll JS,Langford PR

更新日期：2010-05-01 00:00:00

abstract：:Glycerol is a primary energy source for heterotrophic haloarchaea and a major component of "salty" biodiesel waste. Glycerol is catabolized solely by glycerol kinase (encoded by glpK) to glycerol-3-phosphate (G3P) in Haloferax volcanii. Here we characterized the next critical step of this metabolic pathway: the conver...

journal_title：Journal of bacteriology

authors： Rawls KS,Martin JH,Maupin-Furlow JA

更新日期：2011-09-01 00:00:00

abstract：:We measured some of the kinetic parameters of nitrogenase to intact systems of Clostridium pasteurianum and Klebsiella pneumoniae to compare them with the kinetics of the enzyme in vitro. We found that the enzyme showed multiple apparent Km values for acetylene reduction in vivo, as it does in vitro. Carbon monoxide w...

journal_title：Journal of bacteriology

authors： Davis LC,Wang YL

更新日期：1980-03-01 00:00:00

abstract：:We have isolated the gene encoding L-allo-threonine aldolase (L-allo-TA) from Aeromonas jandaei DK-39, a pyridoxal 5'-phosphate (PLP)-dependent enzyme that stereospecifically catalyzes the interconversion of L-allo-threonine and glycine. The gene contains an open reading frame consisting of 1,014 nucleotides correspon...

journal_title：Journal of bacteriology

authors： Liu JQ,Dairi T,Kataoka M,Shimizu S,Yamada H

更新日期：1997-06-01 00:00:00

abstract：:To understand further the role of the flagellum of Vibrio anguillarum in virulence, invasive and adhesive properties of isogenic motility mutants were analyzed by using a chinook salmon embryo cell line. Adhesion was unaffected but invasion of the cell line was significantly decreased in nonmotile or partially motile ...

journal_title：Journal of bacteriology

authors： Ormonde P,Hörstedt P,O'Toole R,Milton DL

更新日期：2000-04-01 00:00:00

abstract：:The cold shock response of Escherichia coli is elicited by downshift of temperature from 37 degrees C to 15 degrees C and is characterized by induction of several cold shock proteins, including CsdA, during the acclimation phase. CsdA, a DEAD-box protein, has been proposed to participate in a variety of processes, suc...

journal_title：Journal of bacteriology

authors： Awano N,Xu C,Ke H,Inoue K,Inouye M,Phadtare S

更新日期：2007-08-01 00:00:00

abstract：:Tectiviridae is a family of tailless bacteriophages with Gram-negative and Gram-positive hosts. The family model PRD1 and its close relatives all infect a broad range of enterobacteria by recognizing a plasmid-encoded conjugal transfer complex as a receptor. In contrast, tectiviruses with Gram-positive hosts are highl...

journal_title：Journal of bacteriology

authors： Kan S,Fornelos N,Schuch R,Fischetti VA

更新日期：2013-10-01 00:00:00

abstract：:Rohlfing, S. R. (Western Reserve University, Cleveland, Ohio), and I. P. Crawford. Purification and characterization of the beta-galactosidase of Aeromonas formicans. J. Bacteriol. 91:1085-1097. 1966.-The beta-galactosidase of Aeromonas formicans was purified by diethylaminoethyl cellulose chromatography and gel filtr...

journal_title：Journal of bacteriology

authors： Rohlfing SR,Crawford IP

更新日期：1966-03-01 00:00:00

abstract：:Genes involved in the biosynthesis of leucine have been mapped in Bacillus megaterium QM B1551, using transducing phage MP13. Mutations were designated leuA, leuB, or leuC on the basis of enzyme assays. Two mutant strains were deficient in the enzyme activities of leuA (alpha-isopropylmalate synthase) and leuC (beta-i...

journal_title：Journal of bacteriology

authors： Garbe JC,Hess GF,Franzen MA,Vary PS

更新日期：1984-02-01 00:00:00

abstract：:An open-reading-frame fragment of a Methylobacterium sp. strain AM1 gene (moxF) encoding a portion of the methanol dehydrogenase structural protein has been used as a hybridization probe to detect similar sequences in a variety of methylotrophic bacteria. This hybridization was used to isolate clones containing putati...

journal_title：Journal of bacteriology

authors： Stephens RL,Haygood MG,Lidstrom ME

更新日期：1988-05-01 00:00:00

abstract：:Colicin E2 was partially purified from Escherichia coli W3110. This preparation was remarkably stabilized by bovine serum albumin in a solution at neutral pH, as shown by dilution experiments and tests on heat stability of colicin. One killing unit of colicin E2 was estimated to correspond to one molecule of colicin E...

journal_title：Journal of bacteriology

authors： Mitsui E,Mizuno D

更新日期：1969-11-01 00:00:00

abstract：:Streptomyces venezuelae ATCC 10595 harbors seven rRNA gene clusters which can be distinguished by BglII digestion. The three rRNA genes present in each set are closely linked with the general structure 16S-23S-5S. We cloned rrnA and sequenced the 16S-23S spacer region and the region downstream of the 5S rRNA gene. No ...

journal_title：Journal of bacteriology

authors： La Farina M,Stira S,Mancuso R,Grisanti C

更新日期：1996-03-01 00:00:00

abstract：:Shewanella oneidensis is a metal reducer that uses the cyclic AMP receptor protein, CRP, to regulate anaerobic respiration. In addition, ArcA(So) is required for anaerobic growth with dimethyl sulfoxide (DMSO) and plays a role in aerobic respiration. The sensor kinase that activates ArcA(So) in S. oneidensis is not kn...

journal_title：Journal of bacteriology

authors： Shroff NP,Charania MA,Saffarini DA

更新日期：2010-06-01 00:00:00

abstract：:A 40-kb region of DNA from Sorangium cellulosum So ce26, which contains polyketide synthase (PKS) genes for synthesis of the antifungal macrolide antibiotic soraphen A, was cloned. These genes were detected by homology to Streptomyces violaceoruber genes encoding components of granaticin PKS, thus extending this power...

journal_title：Journal of bacteriology

authors： Schupp T,Toupet C,Cluzel B,Neff S,Hill S,Beck JJ,Ligon JM

更新日期：1995-07-01 00:00:00

abstract：:Twelve strains of Agrobacterium radiobacter isolated from naturally occurring crown galls or soil were found to be avirulent on sunflower, tomato, Kalanchoe, and carrot. Eleven strains contained plasmids of molecular weights 77 X 10(6) to 182 X 10(6) as determined by electron microscopy. One strain contained only a sm...

journal_title：Journal of bacteriology

authors： Merlo DJ,Nester EW

更新日期：1977-01-01 00:00:00

abstract：:In this report we describe the cloning, organization, and promoter analysis of the Staphylococcus aureus thioredoxin (trxA) and thioredoxin reductase (trxB) genes and their transcription in response to changes in oxygen concentration and to oxidative stress compounds. Northern analysis showed that the S. aureus trxA a...

journal_title：Journal of bacteriology

authors： Uziel O,Borovok I,Schreiber R,Cohen G,Aharonowitz Y

更新日期：2004-01-01 00:00:00

abstract：:Flagellum formation by ubiquinone- and menaquinone-deficient mutant strains of Escherichia coli K-12 was studied under both aerobic and anaerobic growth conditions. Ubiquinone was found to be obligatory for aerobic flagellum formation but could be replaced by menaquinone for anaerobic flagellum formation. A mutant dev...

journal_title：Journal of bacteriology

authors： Hertz R,Bar-Tana J

更新日期：1977-12-01 00:00:00

abstract：:The catalase gene, katA, of the sepiolid squid symbiont Vibrio fischeri has been cloned and sequenced. The predicted amino acid sequence of KatA has a high degree of similarity to the recently defined group III catalases, including those found in Haemophilus influenzae, Bacteroides fragilis, and Proteus mirabilis. Ups...

journal_title：Journal of bacteriology

authors： Visick KL,Ruby EG

更新日期：1998-04-01 00:00:00

abstract：:Salmonella enteritidis was injected intravenously, intraperitoneally, or subcutaneously into specific pathogen-free mice. The number of organisms in the blood, liver, spleen, peritoneal cavity, and draining inguinal lymph node was determined by daily enumeration. Opsonization of the organism with hyperimmune serum inc...

journal_title：Journal of bacteriology

authors： Collins FM

更新日期：1969-02-01 00:00:00

abstract：:Emulsan, the polyanionic emulsifying agent synthesized by Acinetobacter calcoaceticus RAG-1, was depolymerized by an enzyme obtained from a soil bacterium YUV-1. The extracellular emulsan depolymerase was produced when strains RAG-1 and YUV-1 were grown together on agar medium. The enzyme was extracted from the agar a...

journal_title：Journal of bacteriology

authors： Shoham Y,Rosenberg E

更新日期：1983-10-01 00:00:00

abstract：:An RNA polymerase mutant with a single-base-pair change in the rpoC gene affects chromosome initiation control. The mutation, which is recessive, is a G to A transition leading to the substitution of aspartate for glycine at amino acid residue 1033 in the RNA polymerase beta' subunit. The chromosome copy number is inc...

journal_title：Journal of bacteriology

authors： Petersen SK,Hansen FG

更新日期：1991-08-01 00:00:00

abstract：:Production of Bacillus subtilis exoproteases is positively regulated by the DegS-DegU two-component regulatory system and other regulatory factors including DegR and ProB. It was shown that the expression of degR was virtually abolished in a sigD mutant and that the transcriptional initiation site in vivo is preceded ...

journal_title：Journal of bacteriology

authors： Ogura M,Tanaka T

更新日期：1996-01-01 00:00:00

abstract：:The uptake of homologous DNA by Haemophilus influenzae was studied as a function of the proton motive force in completely competent cultures in the pH range of 6 to 8. The composition and magnitude of the proton motive force were varied by using the ionophores valinomycin and nigericin (in the presence of various pota...

journal_title：Journal of bacteriology

authors： Bremer W,Kooistra J,Hellingwerf KJ,Konings WN

更新日期：1984-03-01 00:00:00

abstract：:A d-glucosamine 6-phosphate N-acetyltransferase from Blastocladiella emersonii zoospores was partially purified to a specific activity of 2.41 IU per mg of protein. Its pH optimum was 8.05 and its K(m) values were 2.4 x 10(-4) M d-glucosamine 6-phosphate and 0.38 x 10(-4) M Na(3)S-acetyl coenzyme A. ...

journal_title：Journal of bacteriology

authors： Giddings TH,Cantino EC

更新日期：1974-11-01 00:00:00

abstract：:Antibiotic-resistant Acinetobacter baumannii causes infections that are extremely difficult to treat. A significant role in these resistance profiles is attributed to multidrug efflux pumps, especially those belonging to the resistance-nodulation-cell division (RND) superfamily of transporters. In this study, we analy...

journal_title：Journal of bacteriology

authors： Leus IV,Weeks JW,Bonifay V,Smith L,Richardson S,Zgurskaya HI

更新日期：2018-06-11 00:00:00

abstract：:Chemical, physical, and immunological properties of the envelope antigen of Yersinia pestis strains have been investigated. The antigen consists of two components with isoelectric points (pI) of 4.6 and 4.8. One component (pI 4.6) is a protein bound to a small carbohydrate moiety identified as an oligomeric galactan; ...

journal_title：Journal of bacteriology

authors： Bennett LG,Tornabene TG

更新日期：1974-01-01 00:00:00

abstract：:The exopolysaccharide of Pseudomonas marginalis HT041B has been characterized as a 1,3-linked galactoglucan in which galactose and glucose are in the alpha- and beta-anomeric configurations, respectively. The polysaccharide is substituted with pyruvate at the 4 and 6 positions of galactose and with succinic acid at ei...

journal_title：Journal of bacteriology

authors： Osman SF,Fett WF

更新日期：1989-03-01 00:00:00

abstract：:During depletion of 4.5S RNA, cells of Escherichia coli displayed a heat shock response that was simultaneous with the first detectable effect on ribosome function and before major effects on cell growth. Either 4.5S RNA is involved directly in regulating the heat shock response, or this particular impairment of prote...

journal_title：Journal of bacteriology

authors： Bourgaize DB,Phillips TA,VanBogelen RA,Jones PG,Neidhardt FC,Fournier MJ

更新日期：1990-02-01 00:00:00

abstract：:Although Salmonella typhimurium prefers neutral-pH environments, it can adapt to survive conditions of severe low-pH stress (pH 3.3). The process, termed the acid tolerance response (ATR), includes two distinct stages. The first stage, called pre-acid shock, is induced at pH 5.8 and involves the production of an induc...

journal_title：Journal of bacteriology

authors： Foster JW

更新日期：1993-04-01 00:00:00

abstract：:Nicotinamide adenine dinucleotide (NAD) and reduced NAD (NADH) levels have been measured in bacterial cultures. The cofactors were assayed by using the very sensitive cycling assay described previously by Cartier. Control experiments showed that the level of total NAD(H) falls during harvesting, and so samples were ta...

journal_title：Journal of bacteriology

authors： Wimpenny JW,Firth A

更新日期：1972-07-01 00:00:00