Abstract:
:SecA signal peptide interaction is critical for initiating protein translocation in the bacterial Sec-dependent pathway. Here, we have utilized the recent nuclear magnetic resonance (NMR) and Förster resonance energy transfer studies that mapped the location of the SecA signal peptide-binding site to design and isolate signal peptide-binding-defective secA mutants. Biochemical characterization of the mutant SecA proteins showed that Ser226, Val310, Ile789, Glu806, and Phe808 are important for signal peptide binding. A genetic system utilizing alkaline phosphatase secretion driven by different signal peptides was employed to demonstrate that both the PhoA and LamB signal peptides appear to recognize a common set of residues at the SecA signal peptide-binding site. A similar system containing either SecA-dependent or signal recognition particle (SRP)-dependent signal peptides along with the prlA suppressor mutation that is defective in signal peptide proofreading activity were employed to distinguish between SecA residues that are utilized more exclusively for signal peptide recognition or those that also participate in the proofreading and translocation functions of SecA. Collectively, our data allowed us to propose a model for the location of the SecA signal peptide-binding site that is more consistent with recent structural insights into this protein translocation system.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Grady LM,Michtavy J,Oliver DBdoi
10.1128/JB.06150-11subject
Has Abstractpub_date
2012-01-01 00:00:00pages
307-16issue
2eissn
0021-9193issn
1098-5530pii
JB.06150-11journal_volume
194pub_type
杂志文章abstract::Individually, the ribosomal proteins L1, L23, L36, and S6 are not essential for cell proliferation of Bacillus subtilis, but the absence of any one of these ribosomal proteins causes a defect in the formation of the 70S ribosomes and a reduced growth rate. In mutant strains individually lacking these ribosomal protein...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00212-18
更新日期:2018-08-24 00:00:00
abstract::This study describes the isolation and characterization of a unique class of TolC mutants that, under steady-state growth conditions, secreted normal levels of largely inactive alpha-hemolysin. Unlike the reduced activity in the culture supernatants, the cell-associated hemolytic activity in these mutants was identica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.23.6908-6916.2001
更新日期:2001-12-01 00:00:00
abstract::CatR, a LysR family protein, positively regulates the Pseudomonas putida catBC operon, which is required for growth on benzoate as a sole carbon source. Transcriptional studies show that the catR and catBC promoters are divergent and overlapping by 2 bp. A beta-galactosidase promoter probe vector was constructed to an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.15.4717-4724.1991
更新日期:1991-08-01 00:00:00
abstract::The UspA1 and UspA2 proteins of Moraxella catarrhalis are structurally related, are exposed on the bacterial cell surface, and migrate as very high-molecular-weight complexes in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Previous analysis of uspA1 and uspA2 mutants of M. catarrhalis strain 035E indicat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.5.1364-1373.2000
更新日期:2000-03-01 00:00:00
abstract::Certain mutations in the C-terminal region of the Escherichia coli division inhibitor protein MinC cause loss of function of the division inhibitor by making MinC more sensitive to degradation by Lon protease, implying a possible role for the C-terminal region in regulating the stability and cellular concentration of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.1.175-177.1998
更新日期:1998-01-01 00:00:00
abstract::Aeromonas hydrophila AH-3 lateral flagella are not assembled when bacteria grow in liquid media; however, lateral flagellar genes are transcribed. Our results indicate that A. hydrophila lateral flagellar genes are transcribed at three levels (class I to III genes) and share some similarities with, but have many impor...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01994-12
更新日期:2013-04-01 00:00:00
abstract::Genetic circularity of the Pseudomonas aeruginosa PAO chromosome was demonstrated by a series of two- and three-factor crosses and double-selection experiments with Cma plasmids FP2, FP5, FP110, and R68.45. A range of additional markers, including catabolic markers, were located on the chromosome map. Plasmid FP2, kno...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.1.145-155.1981
更新日期:1981-01-01 00:00:00
abstract::After the demonstration that Stigmatella aurantiaca DW4 secretes an endo-N-acetyl-beta-D-glucosaminidase (ENGase), acting on the di-N-acetylchitobiosyl part of N-linked glycans (S. Bourgerie, Y. Karamanos, T. Grard, and R. Julien, J. Bacteriol. 176:6170-6174, 1994), an ENGase activity having the same substrate specifi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.4.916-920.1995
更新日期:1995-02-01 00:00:00
abstract::Elder, Robert L. (Johns Hopkins University, Baltimore, Md.), and Roland F. Beers, Jr. Nonphotoreactivating repair of ultraviolet light-damaged Micrococcus lysodeikticus cells. J. Bacteriol. 89:1225-1230. 1965.-Micrococcus lysodeikticus cells are highly resistant to ultraviolet radiation. The shape of the dose-survival...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.5.1225-1230.1965
更新日期:1965-05-01 00:00:00
abstract::We purified active hydrogenase from free-living Rhizobium japonicum by affinity chromatography. The uptake hydrogenase of R. japonicum has been treated previously as an oxygen-sensitive protein. In this purification, however, reducing agents were not added nor was there any attempt to exclude oxygen. In fact, the addi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.3.795-800.1986
更新日期:1986-06-01 00:00:00
abstract::Deoxyribonucleic acid (DNA), pulse labeled after ultraviolet irradiation of excision-defective mutants of Haemophilus influenzae, is of lower single strand molecular weight than that of unirradiated cells but approaches the size of DNA from unirradiated cells upon further incubation in growth medium. This gap-filling ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.176-181.1975
更新日期:1975-10-01 00:00:00
abstract::The syrA and syrB genes involved in syringomycin production in Pseudomonas syringae pv. syringae B301D were identified from an EcoRI-pLAFR3 cosmid library and then physically and functionally analyzed in relation to plant pathogenicity. Homologous recombination of the genes required for syringomycin production from co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5680-5688.1988
更新日期:1988-12-01 00:00:00
abstract::The reproduction and morphogenesis of Rickettsiella chironomi, an unusual procaryotic parasite of midge larvae, was studied with electron microscopy. The morphogenic cycle was similar to that found in chlamydia and consisted of four major cell types: (i) medium-sized spherical initial bodies (ca. 1 micrometers in diam...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.995-1002.1980
更新日期:1980-08-01 00:00:00
abstract::The McrB restriction system of Escherichia coli K-12 is responsible for the biological inactivation of foreign DNA that contains 5-methylcytosine residues (E. A. Raleigh and G. Wilson, Proc. Natl. Acad. Sci. USA 83:9070-9074, 1986). Within the McrB region of the chromosome is the mcrB gene, which encodes a protein of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.1974-1981.1989
更新日期:1989-04-01 00:00:00
abstract::A mutational analysis of lesion-forming ability was undertaken in Pseudomonas syringae pv. syringae B728a, causal agent of bacterial brown spot disease of bean. Following a screen of 6,401 Tn5-containing derivatives of B728a on bean pods, 26 strains that did not form disease lesions were identified. Nine of the mutant...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.7.2247-2258.1997
更新日期:1997-04-01 00:00:00
abstract::The rate at which the peptidoglycan precursor meso-diaminopimelic acid (DAP) is incorporated into the cell wall of Escherichia coli cells was determined by pulse-label experiments. For different E. coli strains, the incorporation rate was compared with the rate of uptake of DAP into the cell. With E. coli W7, a dap ly...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.1.331-337.1985
更新日期:1985-10-01 00:00:00
abstract::The products of the bvgAS locus coordinately regulate expression of the Bordetella pertussis virulence regulon in response to environmental signals. Transcription of bvgAS-activated genes is nearly eliminated by several modulating conditions, including the presence of sulfate anion or nicotinic acid and growth at low ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.3.970-979.1992
更新日期:1992-02-01 00:00:00
abstract::Bacillus subtilis has developed an intricate signal transduction cascade to respond to the imposition of a variety of stresses on the cell. Reversible protein phosphorylation and the formation of alternative protein-protein complexes modulate the activity of sigma(B), the RNA polymerase sigma factor subunit responsibl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00892-06
更新日期:2006-11-01 00:00:00
abstract::The A-subunit polypeptides of Shiga toxin, the Shiga-like toxins (SLTs), and the plant lectin ricin inactivate eucaryotic ribosomes by enzymatically depurinating 28S rRNA. Comparison of the amino acid sequences of the members of the Shiga toxin family and ricin revealed two regions of significant homology that lie wit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.6.3346-3350.1990
更新日期:1990-06-01 00:00:00
abstract::West Indian spiny lobsters, Panulirus argus, synthesized a hemolymph bactericidin after being injected with killed suspensions of gram-negative bacillus EMB-1 isolated from the normal gut of this lobster. To study differences between the primary response and secondary response, animals were given a primary antigen inj...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.3.943-946.1969
更新日期:1969-06-01 00:00:00
abstract::The vir locus of Bordetella pertussis apparently encodes a trans-acting positive regulator that is required for the coordinate expression of genes associated with virulence: pertussis toxin, filamentous hemagglutinin (FHA), hemolysin, and adenylate cyclase toxin. DNA clones of vir and of genes required for the synthes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.7.2904-2913.1988
更新日期:1988-07-01 00:00:00
abstract::The ToxR protein of Vibrio cholerae is an integral membrane protein that coordinately regulates the expression of virulence genes required for successful infection. ToxR has been shown to bind directly to and activate transcription of the cholera toxin (ctx) promoter. Within the amino-terminal cytoplasmic region of To...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.21.6807-6814.1992
更新日期:1992-11-01 00:00:00
abstract::Marine hydrocarbonoclastic bacteria, like Alcanivorax borkumensis, play a globally important role in bioremediation of petroleum oil contamination in marine ecosystems. Accumulation of storage lipids, serving as endogenous carbon and energy sources during starvation periods, might be a potential adaptation mechanism f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01292-06
更新日期:2007-02-01 00:00:00
abstract::In Escherichia coli, the phn operon encodes proteins responsible for the uptake and breakdown of phosphonates. The C-P (carbon-phosphorus) lyase enzyme encoded by this operon which catalyzes the cleavage of C-P bonds in phosphonates has been recalcitrant to biochemical characterization. To advance the understanding of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.2.389-395.1999
更新日期:1999-01-01 00:00:00
abstract::Quie, Paul G. (University of Minnesota, Minneapolis) and Lewis W. Wannamaker. Staphylococcal Muller phenomenon: relationship to the plasminogen-plasmin system. J. Bacteriol. 82:770-783. 1961.-The staphylococcal factor that produces particulate proteolysis (Muller phenomenon) in whole blood or hemoglobinserum agar plat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.5.770-783.1961
更新日期:1961-11-01 00:00:00
abstract::Spores of Bacillus subtilis have a thick outer layer of relatively insoluble protein called the coat, which protects spores against a number of treatments and may also play roles in spore germination. However, elucidation of precise roles of the coat in spore properties has been hampered by the inability to prepare sp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00896-08
更新日期:2008-10-01 00:00:00
abstract::Razin, S. (University of Connecticut, Storrs), M. E. Tourtellotte, R. N. McElhaney, and J. D. Pollack. Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells. J. Bacteriol. 91:609-616. 1966.-Lipid composition of Mycoplasma laidlawii membranes could be significantly changed by var...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.609-616.1966
更新日期:1966-02-01 00:00:00
abstract::Salmonella typhimurium invasion of nonphagocytic cells requires the expression of a type III secretion system (TTSS) encoded within Salmonella pathogenicity island 1 (SPI1). TTSS gene transcription is activated in response to environmental signals and requires transcriptional regulators encoded within (HilA) and outsi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.10.3096-3104.1999
更新日期:1999-05-01 00:00:00
abstract::Legionella pneumophila expresses two catalase-peroxidase enzymes that exhibit strong peroxidatic but weak catalatic activities, suggesting that other enzymes participate in decomposition of hydrogen peroxide (H2O2). Comparative genomics revealed that L. pneumophila and its close relative Coxiella burnetii each contain...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00635-06
更新日期:2006-09-01 00:00:00
abstract::The 6th American Society for Microbiology Conference on Cell-Cell Communication in Bacteria convened from 16 to 19 October 2017 in Athens, GA. In this minireview, we highlight some of the research presented at that meeting that addresses central questions emerging in the field, including the following questions. How a...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00291-18
更新日期:2018-06-25 00:00:00