Mutational analysis of the Shiga toxin and Shiga-like toxin II enzymatic subunits.

abstract：:The activity of beta-glucosidase (EC 3.2.1.21) in extracts of Dictyostelium discoideum was investigated. The specific activity increased early in development, declined during pseudoplasmodium formation, and increased again during sorocarp formation. The beta-glucosidase which was present in growing amoebae and during ...

journal_title：Journal of bacteriology

authors： Coston MB,Loomis WF Jr

更新日期：1969-12-01 00:00:00

abstract：UNLABELLED:Streptococcus pneumoniae is able to integrate exogenous DNA into its genome by natural genetic transformation. Transient accumulation of high levels of the only S. pneumoniae alternative σ factor is insufficient for development of full competence without expression of a second competence-specific protein, Co...

journal_title：Journal of bacteriology

authors： Tovpeko Y,Bai J,Morrison DA

更新日期：2016-08-11 00:00:00

abstract：:The Escherichia coli mutant Y16, which shows thermosensitive colony formation and filamentation with reduced amounts of penicillin-binding protein 3 (PBP3), has mutations in the ftsI gene encoding PBP3 and in the ftsH gene. The ftsI mutation markedly reduces the amount of PBP3 at 42 degrees C, whereas the amount of th...

journal_title：Journal of bacteriology

authors： Begg KJ,Tomoyasu T,Donachie WD,Khattar M,Niki H,Yamanaka K,Hiraga S,Ogura T

更新日期：1992-04-01 00:00:00

abstract：:The Neurospora crassa assimilatory nitrite reductase structural gene, nit-6, has been isolated. A cDNA library was constructed from poly(A)+ RNA isolated from Neurospora mycelia in which nitrate assimilation had been induced. This cDNA was ligated into lambda ZAP II (Stratagene) and amplified. This library was then sc...

journal_title：Journal of bacteriology

authors： Exley GE,Colandene JD,Garrett RH

更新日期：1993-04-01 00:00:00

abstract：:The cir gene, which encodes the colicin I receptor protein and is regulated by both cellular iron content and growth temperature, was cloned into a multicopy-number plasmid. Physical mapping and complementation analysis established the position of cir between mgl and nfo on the Escherichia coli chromosome. A gene enco...

journal_title：Journal of bacteriology

authors： Griggs DW,Tharp BB,Konisky J

更新日期：1987-12-01 00:00:00

abstract：:Fine details of the internal and external morphology of Cryptococcus neoformans as seen in ultrathin sections are described and illustrated with electron micrographs. The capsule characteristic of this species contained microfibrils (30 to 40 A in diameter) that appeared to radiate from the cell wall and to coil and i...

journal_title：Journal of bacteriology

authors： Edwards MR,Gordon MA,Lapa EW,Ghiorse WC

更新日期：1967-09-01 00:00:00

abstract：:During meiosis and sporulation in Saccharomyces cerevisiae, the recessive genetic marker for cycloheximide resistance, believed to be due to an altered ribosomal protein (C. S. McLaughlin, p. 815-827, in M. Nomura et al., ed., Ribosomes, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.), is expressed as early a...

journal_title：Journal of bacteriology

authors： Emanuel JR,Magee PT

更新日期：1981-03-01 00:00:00

abstract：:Banerjea, A. (Rocky Mountain Laboratory, Hamilton, Mont.) and J. Munoz. Antigens of Bordetella pertussis. II, Purification of heat-labile toxin. J. Bacteriol. 84:269-274. 1962.-A mild method of separating heat-labile toxin of Bordetella pertussis from other cellular components is described; it consists of absorbing to...

journal_title：Journal of bacteriology

authors： BANERJEA A,MUNOZ J

更新日期：1962-08-01 00:00:00

abstract：:Cosmid B577, a member of the collection of ordered clones corresponding to the genome of Mycobacterium leprae, contains a gene, provisionally called pon1, that encodes an 821-amino-acid-residue high-molecular-mass class A penicillin-binding protein, provisionally called PBP1. With similar amino acid sequences and modu...

journal_title：Journal of bacteriology

authors： Basu J,Mahapatra S,Kundu M,Mukhopadhyay S,Nguyen-Distèche M,Dubois P,Joris B,Van Beeumen J,Cole ST,Chakrabarti P,Ghuysen JM

更新日期：1996-03-01 00:00:00

abstract：:Rickettsia heilongjiangensis is an emerging tick-transmitted human pathogen causing far-Eastern spotted fever. Here we report the complete sequence and the main features of the genome of R. heilongjiangensis (strain 054). ...

journal_title：Journal of bacteriology

authors： Duan C,Tong Y,Huang Y,Wang X,Xiong X,Wen B

更新日期：2011-10-01 00:00:00

abstract：:The citrate transporter CitP of lactic acid bacteria catalyzes electrogenic precursor-product exchange of citrate versus L-lactate during citrate-glucose cometabolism. In the absence of sugar, L-lactate is replaced by the metabolic intermediates/end products pyruvate, α-acetolactate, and acetate. In this study, the bi...

journal_title：Journal of bacteriology

authors： Pudlik AM,Lolkema JS

更新日期：2012-07-01 00:00:00

abstract：:The gene encoding the general stress transcription factor sigmaB in the gram-positive bacterium Listeria monocytogenes was isolated with degenerate PCR primers followed by inverse PCR amplification. Evidence for gene identification includes the following: (i) phylogenetic analyses of reported amino acid sequences for ...

journal_title：Journal of bacteriology

authors： Wiedmann M,Arvik TJ,Hurley RJ,Boor KJ

更新日期：1998-07-01 00:00:00

abstract：:Eubacterium sp. strain VPI 12708 is an anaerobic intestinal bacterium that has inducible bile acid 7-dehydroxylation activity. At least four new polypeptides were synthesized after addition of primary bile acids to the growth medium. One of these, of molecular weight 27,000 (P-27), was shown to be involved in the 7-de...

journal_title：Journal of bacteriology

authors： Coleman JP,White WB,Lijewski M,Hylemon PB

更新日期：1988-05-01 00:00:00

abstract：:Previous studies have indicated that a single enzyme, "kynureninase," catalyzes the reactions of l-kynurenine to anthranilate and l-3-hydroxykynurenine to 3-hydroxyanthranilate in Neurospora crassa and in other organisms. The present report describes separate enzymes which catalyze these reactions in N. crassa. The fi...

journal_title：Journal of bacteriology

authors： Gaertner FH,Cole KW,Welch GR

更新日期：1971-11-01 00:00:00

abstract：:Hydrolytic deamination of cytosine to uracil in DNA is increased in organisms adapted to high temperatures. Hitherto, the uracil base excision repair (BER) pathway has only been described in two archaeons, the crenarchaeon Pyrobaculum aerophilum and the euryarchaeon Archaeoglobus fulgidus, which are hyperthermophiles ...

journal_title：Journal of bacteriology

authors： Moen MN,Knævelsrud I,Haugland GT,Grøsvik K,Birkeland NK,Klungland A,Bjelland S

更新日期：2011-09-01 00:00:00

abstract：:Sato, Ichiei (School of Medicine, Gunma University, Maebashi, Japan), Tokumitsu Tanaka, Kazuko Saito, and Susumu Mitsuhashi. Inhibition of Salmonella enteritidis ingested in mononuclear phagocytes from liver and subcutaneous tissue of mice immunized with live vaccine. J. Bacteriol. 83:1306-1312. 1962.-In our earlier s...

journal_title：Journal of bacteriology

authors： SATO I,TANAKA T,SAITO K,MITSUHASHI S

更新日期：1962-06-01 00:00:00

abstract：:Oligopeptidase B (OpdB) is a serine peptidase broadly distributed among unicellular eukaryotes, gram-negative bacteria, and spirochetes which has emerged as an important virulence factor and potential therapeutic target in infectious diseases. We report here the cloning and expression of the opdB homologue from Salmon...

journal_title：Journal of bacteriology

authors： Morty RE,Fülöp V,Andrews NW

更新日期：2002-06-01 00:00:00

abstract：:MreC and MreD, along with the actin homologue MreB, are required to maintain the shape of rod-shaped bacteria. The depletion of MreCD in rod-shaped bacteria leads to the formation of spherical cells and the accumulation of suppressor mutations. Ovococcus bacteria, such as Streptococcus pneumoniae, lack MreB homologues...

journal_title：Journal of bacteriology

authors： Land AD,Winkler ME

更新日期：2011-08-01 00:00:00

abstract：:A three-dimensional tracking microscope was used to quantify the effects of temperature (50 to 80 degrees C) and pH (2 to 4) on the motility of Sulfolobus acidocaldarius, a thermoacidophilic archaeon. Swimming speed and run time increased with temperature but remained relatively unchanged with increasing pH. These res...

journal_title：Journal of bacteriology

authors： Lewus P,Ford RM

更新日期：1999-07-01 00:00:00

abstract：:A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-P(BAD) control element into the bacterial chromosome allows dynami...

journal_title：Journal of bacteriology

authors： McKinney J,Guerrier-Takada C,Galán J,Altman S

更新日期：2002-11-01 00:00:00

abstract：:Species of marine bacteria belonging to the genus Beneckea and strains of Photobacterium fischeri were negatively stained and examined by means of the electron microscope to determine the structure and arrangement of their flagella. All of the species of the genus Beneckea had single, polar, sheathed flagella when gro...

journal_title：Journal of bacteriology

authors： Allen RD,Baumann P

更新日期：1971-07-01 00:00:00

abstract：:Shigella flexneri causes bacillary dysentery by invading epithelial cells of the colonic mucosa. The invasion process requires the synthesis and secretion of the virulence plasmid-encoded Ipa proteins. Using TnphoA mutagenesis, we have identified two virulence plasmid genes, mxiJ and mxiM, that encode proteins exporte...

journal_title：Journal of bacteriology

authors： Allaoui A,Sansonetti PJ,Parsot C

更新日期：1992-12-01 00:00:00

abstract：:Thiobacillus neapolitanus, when grown in continuous culture with thiosulfate limiting growth, possessed an apparent maximal molar growth yield of 8.0 g (dry weight) per mole of thiosulfate. The substrate requirement for energy of maintenance was the highest yet reported, amounting to 21.8 mmoles of thiosulfate per g p...

journal_title：Journal of bacteriology

authors： Hempfling WP,Vishniac W

更新日期：1967-03-01 00:00:00

abstract：:Expression plasmids containing various portions of araFGH operon sequences were assayed for their ability to facilitate the high-affinity L-arabinose transport process in a strain lacking the chromosomal copy of this operon. Accumulation studies demonstrated that the specific induction of all three operon coding seque...

journal_title：Journal of bacteriology

authors： Horazdovsky BF,Hogg RW

更新日期：1989-06-01 00:00:00

abstract：:Sequence flanking the bfpA locus on the enteroadherent factor plasmid of the enteropathogenic Escherichia coli (EPEC) strain B171-8 (O111:NM) was obtained to identify genes that might be required for bundle-forming pilus (BFP) biosynthesis. Deletion experiments led to the identification of a contiguous cluster of at l...

journal_title：Journal of bacteriology

authors： Sohel I,Puente JL,Ramer SW,Bieber D,Wu CY,Schoolnik GK

更新日期：1996-05-01 00:00:00

abstract：:We have cloned and sequenced a 505-base-pair (bp) segment of DNA situated upstream of mvaA, the structural gene for (S)-3-hydroxy-3-methylglutaryl coenzyme A reductase (EC 1.1.1.88) of Pseudomonas mevalonii. The DNA segment that we characterized includes the promoter region for the mva operon. Nuclease S1 mapping and ...

journal_title：Journal of bacteriology

authors： Wang YL,Beach MJ,Rodwell VW

更新日期：1989-10-01 00:00:00

abstract：:Razin, S. (University of Connecticut, Storrs), M. E. Tourtellotte, R. N. McElhaney, and J. D. Pollack. Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells. J. Bacteriol. 91:609-616. 1966.-Lipid composition of Mycoplasma laidlawii membranes could be significantly changed by var...

journal_title：Journal of bacteriology

authors： Razin S,Tourtellotte ME,McElhaney RN,Pollack JD

更新日期：1966-02-01 00:00:00

abstract：:This study examines certain membrane-related aspects of oxygen poisoning in Escherichia coli K1060 (fabB fadE lacI) and its parent strain, K-12 Ymel. Cells were grown to exponential or stationary phase in a minimal medium and exposed to air plus 300 lb/in2 of O2 as a suspension in minimal salts. After an initial lag, ...

journal_title：Journal of bacteriology

authors： Harley JB,Santangelo GM,Rasmussen H,Goldfine H

更新日期：1978-06-01 00:00:00

abstract：:Dominance tests in diploids have confirmed that the product of the tyrR gene is involved in a negative control system affecting the synthesis of both 3-deoxy-d-arabinoheptulosonic acid 7-phosphate (DAHP) synthetase (tyr) and DAHP synthetase (phe). Some tyrR mutants are derepressed for the synthesis of both DAHP synthe...

journal_title：Journal of bacteriology

authors： Im SW,Pittard J

更新日期：1973-09-01 00:00:00

abstract：UNLABELLED:The Mycobacterium tuberculosis exported repetitive protein (RvErp) is a crucial virulence-associated factor as determined by its role in the survival and multiplication of mycobacteria in cultured macrophages and in vivo Although attempts have been made to understand the function of Erp protein, its exact ro...

journal_title：Journal of bacteriology

authors： Ganaie AA,Trivedi G,Kaur A,Jha SS,Anand S,Rana V,Singh A,Kumar S,Sharma C

更新日期：2016-09-22 00:00:00