Abstract:
:In Escherichia coli, the phn operon encodes proteins responsible for the uptake and breakdown of phosphonates. The C-P (carbon-phosphorus) lyase enzyme encoded by this operon which catalyzes the cleavage of C-P bonds in phosphonates has been recalcitrant to biochemical characterization. To advance the understanding of this enzyme, we have cloned DNA from Rhizobium (Sinorhizobium) meliloti that contains homologues of the E. coli phnG, -H, -I, -J, and -K genes. We demonstrated by insertional mutagenesis that the operon from which this DNA is derived encodes the R. meliloti C-P lyase. Furthermore, the phenotype of this phn mutant shows that the C-P lyase has a broad substrate specificity and that the organism has another enzyme that degrades aminoethylphosphonate. A comparison of the R. meliloti and E. coli phn genes and their predicted products gave new information about C-P lyase. The putative R. meliloti PhnG, PhnH, and PhnK proteins were overexpressed and used to make polyclonal antibodies. Proteins of the correct molecular weight that react with these antibodies are expressed by R. meliloti grown with phosphonates as sole phosphorus sources. This is the first in vivo demonstration of the existence of these hitherto hypothetical Phn proteins.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Parker GF,Higgins TP,Hawkes T,Robson RLdoi
10.1128/JB.181.2.389-395.1999subject
Has Abstractpub_date
1999-01-01 00:00:00pages
389-95issue
2eissn
0021-9193issn
1098-5530journal_volume
181pub_type
杂志文章abstract::The bacterial phosphoenolpyruvate phosphotransferase system (PTS) is a highly conserved phosphotransfer cascade that participates in the transport and phosphorylation of selected carbohydrates and modulates many cellular functions in response to carbohydrate availability. It plays a role in the virulence of many bacte...
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journal_title:Journal of bacteriology
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doi:10.1128/JB.113.1.433-444.1973
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abstract::lac operon fusions to DNA damage-inducible (din) loci were generated in Salmonella typhimurium LT2. Many of these din fusions were efficiently repressed by cloned Escherichia coli LexA, while others were not; all required RecA for induction. Several din fusions exhibited strong inducibility and will be useful in devel...
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更新日期:1994-10-01 00:00:00
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更新日期:1995-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-05-01 00:00:00
abstract::The function of SufI, a well-studied substrate of the TatABC translocase in Escherichia coli, is not known. It was earlier implicated in cell division, based on the finding that multiple copies of sufI suppressed the phenotypes of cells with mutations in ftsI (ftsI23), which encodes a divisomal transpeptidase. Recentl...
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pub_type: 杂志文章
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更新日期:2007-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.171.8.4385-4394.1989
更新日期:1989-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.159.2.803-804.1984
更新日期:1984-08-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2012-09-01 00:00:00
abstract::The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.15.4544-4548.1995
更新日期:1995-08-01 00:00:00
abstract::The ugp operon of Escherichia coli includes genes involved in the uptake of sn-glycerol-3-phosphate and glycerophosphoryl diesters and belongs to the pho regulon which is induced by phosphate limitation. This operon has two transcriptional initiation sites, as determined by S1 nuclease mapping of the in vivo transcrip...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.549-558.1991
更新日期:1991-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00005-09
更新日期:2009-05-01 00:00:00
abstract::Cell division of Escherichia coli K-12 strain PA3092 was inhibited by the addition of adenosine 3',5'-phosphate (cAMP), and the cellular morphology was changed from rods into filaments. Nucleoids in the filaments were regularly distributed and septum formation was perfectly inhibited. This inhibition of cell division ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.3.1105-1109.1981
更新日期:1981-09-01 00:00:00
abstract::H10407 is a strain of enterotoxigenic Escherichia coli (ETEC) that utilizes CFA/I pili to adhere to surfaces of the small intestine, where it elaborates toxins that cause profuse watery diarrhea in humans. Expression of the CFA/I pilus is positively regulated at the level of transcription by CfaD, a member of the AraC...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00131-07
更新日期:2007-07-01 00:00:00
abstract::Carbon catabolite repression of maltase gene expression is brought about by the addition of glucose, resulting in a drastic inhibition of the induction of maltase. When added to induced cells, glucose leads to the inhibition of maltase synthesis within 30 min, which can be accounted for by the disappearance of hybridi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.301-307.1983
更新日期:1983-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.134.3.744-750.1978
更新日期:1978-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.115.3.1212-1214.1973
更新日期:1973-09-01 00:00:00
abstract::Listeria monocytogenes is a facultative intracellular gram-positive bacterium responsible for severe opportunistic infections in humans and animals. We had previously identified a gene encoding a putative UDP-N-acetylglucosamine 2-epimerase, a precursor of the teichoic acid linkage unit, in the genome of L monocytogen...
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更新日期:2006-09-01 00:00:00
abstract::A compound, isolated from the culture fluid of a nadC auxotroph of Escherichia coli grown in a minimal medium, supports the growth of both a nadA and a nadB mutant. This metabolite exhibits an ultraviolet light absorption spectrum and a mass spectrum, different from quinolinic acid. This compound may be the precursor ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.1.212-218.1975
更新日期:1975-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1985-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-06-01 00:00:00
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更新日期:2007-10-01 00:00:00
abstract::Glucose-1-(14)C and acetylglucosamine-1-(14)C were added singly and together with equal amounts of the unlabeled reciprocal to Brain Heart Infusion and used for the culture of Streptococcus pyogenes. The labeling pattern of the rhamnose, glucosamine, and muramic acid in the cell wall supported an intermediary role for...
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pub_type: 杂志文章
doi:10.1128/JB.94.4.963-965.1967
更新日期:1967-10-01 00:00:00
abstract::Strains of Bacillus thuringiensis such as B. thuringiensis subsp. israelensis (ONR-60A) and B. thuringiensis subsp. morrisoni (PG-14) pathogenic for mosquito larvae produce a complex parasporal body consisting of several protein endotoxins synthesized during sporulation that form an aggregate of crystalline inclusions...
journal_title:Journal of bacteriology
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更新日期:2012-03-01 00:00:00
abstract::Gaines, Sidney (Walter Reed Army Institute of Research, Washington, D.C.), Julius A. Currie, and Joseph G. Tully. Factors affecting formation of incomplete Vi antibody in mice. J. Bacteriol. 90:635-642. 1965.-Single immunizing doses of purified Vi antigen elicited complete and incomplete Vi antibodies in BALB/c mice, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.3.635-642.1965
更新日期:1965-09-01 00:00:00
abstract::Significant changes in the relative proportions of the individual polar lipids of two strains of Bacillus subtilis were observed when the pH of their chemostat cultures was varied. In phosphate- and magnesium-limited cultures of B. subtilis var. niger NCIB 8058. lysylphosphatidylglycerol was present in higher proporti...
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pub_type: 杂志文章
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更新日期:1974-12-01 00:00:00