Effect of ribonuclease on the association of deoxyribonucleic acid with the membrane in Escherichia coli.

Abstract:

:The Mg-2+-Sarkosyl crystals (M band) procedure was used to study the effect of ribonuclease (RNase) A on the association of Escherichia coli deoxyribonucleic acid (DNA) with membrane. Incubation of gently prepared cell extracts with RNase results in the release of DNA from membrane. This effect appears to result from the activation, by RNase, of endonuclease I and subsequent limited activity of this deoxyribonuclease. In support of this explanation, it is demonstrated (i) that the extent of the RNase-induced loss of DNA from membrane is directly correlated with the endogenous level of endonuclease I, and (ii) that endonucleolytic activity occurs when gently lysed cell preparations are incubated in the presence of RNase.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

McIntosh MA,Earhart CF

doi

10.1128/JB.122.2.592-598.1975

keywords:

subject

Has Abstract

pub_date

1975-05-01 00:00:00

pages

592-8

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

122

pub_type

杂志文章
  • Positive selection acting on a surface membrane protein of the plant-pathogenic phytoplasmas.

    abstract::Phytoplasmas are plant-pathogenic bacteria that cause numerous diseases. This study shows a strong positive selection on the phytoplasma antigenic membrane protein (Amp). The ratio of nonsynonymous to synonymous substitutions was >1 with all the methods we tested. The clear positive selections imply an important biolo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.9.3424-3428.2006

    authors: Kakizawa S,Oshima K,Jung HY,Suzuki S,Nishigawa H,Arashida R,Miyata S,Ugaki M,Kishino H,Namba S

    更新日期:2006-05-01 00:00:00

  • The promoter for a sporulation gene in the spoIVC locus of Bacillus subtilis and its use in studies of temporal and spatial control of gene expression.

    abstract::We have identified the transcription start site and regulatory region governing the expression of a sporulation gene in the spoIVC locus of Bacillus subtilis. Efficient expression and developmental regulation of this gene was controlled from a promoter region that extended no more than 110 base pairs upstream and no m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.8.3513-3522.1988

    authors: Kunkel B,Sandman K,Panzer S,Youngman P,Losick R

    更新日期:1988-08-01 00:00:00

  • Discovery of a nonclassical siderophore, legiobactin, produced by strains of Legionella pneumophila.

    abstract::The mechanisms by which Legionella pneumophila, a facultative intracellular parasite and the agent of Legionnaires' disease, acquires iron are largely unexplained. Several earlier studies indicated that L. pneumophila does not elaborate siderophores. However, we now present evidence that supernatants from L. pneumophi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.3.749-757.2000

    authors: Liles MR,Scheel TA,Cianciotto NP

    更新日期:2000-02-01 00:00:00

  • Structural diversity among methanofurans from different methanogenic bacteria.

    abstract::An examination of the methanofurans isolated from a wide range of methanogenic bacteria and from Archaeoglobus fulgidus has revealed at least five chromatographically distinct methanofurans. Bacteria from each major genus of methanogenic bacteria have been found to contain a chemically different methanofuran. The natu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.10.4594-4597.1988

    authors: White RH

    更新日期:1988-10-01 00:00:00

  • Characterization and DNA sequence of the mobilization region of pLV22a from Bacteroides fragilis.

    abstract::A 4.2-kb plasmid (pLV22a) native to Bacteroides fragilis LV22 became fused to a transfer-deficient Bacteroides spp.-Escherichia coli shuttle vector by an inverse transposition event, resulting in a transferrable phenotype. The transfer phenotype was attributable to pLV22a, which was also capable of mobilization within...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.15.4466-4473.1995

    authors: Novicki TJ,Hecht DW

    更新日期:1995-08-01 00:00:00

  • Observations on replication and cell division in synchronous cultures of the blue-green alga Anacystis nidulans.

    abstract::A second synchrony induction treatment, half-way through the deoxyribonucleic acid replication process of an already synchronized culture, resulted in defective subsequent cell growth. This supports a previous explanation for the partial loss of viability after synchrony induction. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.107.2.583-584.1971

    authors: Herdman M,Carr NG

    更新日期:1971-08-01 00:00:00

  • Expression of a uniquely regulated extracellular polysaccharide confers a large-capsule phenotype to Bacteroides fragilis.

    abstract::Bacteroides fragilis synthesizes eight distinct capsular polysaccharides, more than any described bacterium outside the order Bacteroidales. Here, we show that this organism also produces a high-molecular-weight extracellular polysaccharide (EPS). Expression of the EPS results in the formation of a large polysaccharid...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01519-07

    authors: Chatzidaki-Livanis M,Coyne MJ,Roche-Hakansson H,Comstock LE

    更新日期:2008-02-01 00:00:00

  • Pyrimidine biosynthesis in Lactobacillus leichmannii.

    abstract::Tracer studies of pyrimidine biosynthesis in Lactobacillus leichmannii (ATCC 7830) indicated that, while aspartate is utilized in the usual manner, the guanido carbon of arginine, rather than carbon dioxide, is utilized as a pyrimidine precursor. The guanido carbon of arginine also contributes, to some extent, to the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.4.1249-1254.1968

    authors: Hutson JY,Downing M

    更新日期:1968-10-01 00:00:00

  • Defined medium for growth of Haemophilus influenzae.

    abstract::A chemically defined medium (MI(c)) is described in which cells of Haemophilus influenzae Rd grow rapidly (generation time, 35 +/- 5 min) and reach a stationary level of 10(10) cells/ml. Our strain of cells grown in this medium developed high levels of competence when transferred to another medium designed for that pu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.2.513-516.1970

    authors: Herriott RM,Meyer EY,Vogt M,Modan M

    更新日期:1970-02-01 00:00:00

  • Genes for the establishment and maintenance of lysogeny by the temperate coliphage 186.

    abstract::To identify the genes in coliphage 186 that are required for lysogeny, we isolated clear-plaque mutants. Complementation studies and DNA sequencing identified two genes, the cI gene for the immunity maintenance repressor and the cII gene, which is required only for the establishment of lysogeny. One mutant carried a c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.16.5286-5288.1993

    authors: Lamont I,Richardson H,Carter DR,Egan JB

    更新日期:1993-08-01 00:00:00

  • Identification of a target gene and activating stimulus for the YpdA/YpdB histidine kinase/response regulator system in Escherichia coli.

    abstract::Escherichia coli contains 30 two-component systems (TCSs), each consisting of a histidine kinase and a response regulator. Whereas most TCSs are well characterized in this model organism, little is known about the YpdA/YpdB system. To identify YpdB-regulated genes, we compared the transcriptomes of E. coli cells overp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02051-12

    authors: Fried L,Behr S,Jung K

    更新日期:2013-02-01 00:00:00

  • Mechanism of acetate synthesis from CO2 by Clostridium acidiurici.

    abstract::Total synthesis of acetate from CO2 by Clostridium acidiurici during fermentations of hypoxanthine has been shown to involve synthesis of glycine from methylenetetrahydrofolate, CO2, and NH3. The glycine is converted to serine by the addition of methylenetetrahydrofolate, and the resulting serine is converted to pyruv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.2.468-478.1979

    authors: Waber LJ,Wood HG

    更新日期:1979-11-01 00:00:00

  • Catabolite-repression-like phenomenon in Rhizobium meliloti.

    abstract::We report a phenomenon similar to catabolite repression in Rhizobium meliloti. Succinate, which allows the highest observed rate of growth of R. meliloti, caused an immediate reduction of beta-galactosidase activity when added to cells growing in lactose. A Lac- mutant was unaltered in nodulation and nitrogen fixation...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.3.1197-1200.1978

    authors: Ucker DS,Signer ER

    更新日期:1978-12-01 00:00:00

  • Chimeric streptococcal plasmids and their use as molecular cloning vehicles in Streptococcus sanguis (Challis).

    abstract::Chimeric plasmids, which were useful as cloning vehicles in a Streptococcus sanguis (Challis) host vector system, have been constructed. By using three different strategies of restriction endonuclease digestion and ligation, a deoxyribonucleic acid (DNA) fragment bearing an erythromycin resistance determinant was liga...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.3.1425-1435.1980

    authors: Macrina FL,Jones KR,Wood PH

    更新日期:1980-09-01 00:00:00

  • Rhizobium meliloti insertion element ISRm2 and its use for identification of the fixX gene.

    abstract::Two of the three plasmids of the wild-type Rhizobium meliloti 41 (pRme41a and pRme41c) carry a copy of ISRm2, a 2.7-kilobase-long transposable element. ISRm2 is terminated by 22-base-pair (bp) inverted repeat sequences, exhibiting some homology to the inverted repeats of elements generating 9-bp target sequence duplic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.4.1403-1409.1987

    authors: Dusha I,Kovalenko S,Banfalvi Z,Kondorosi A

    更新日期:1987-04-01 00:00:00

  • Isolation and characterization of a Saccharomyces cerevisiae mutant deficient in pyruvate kinase activity.

    abstract::A mutant of the yeast Saccharomyces cerevisiae that is deficient in pyruvate kinase activity has been isolated. The mutant strain is capable of growth when supplied with lactate as the carbon source but not capable of growth when supplied with dextrose or other fermentable sugars or glycerol as the carbon source. Gene...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.1.232-241.1977

    authors: Sprague GF Jr

    更新日期:1977-04-01 00:00:00

  • Inhibition of the development of the spore septum and membranes in Bacillus cereus by beta-phenethyl alcohol.

    abstract::Remsen, C. C. (Syracuse University, Syracuse, N.Y.), D. G. Lundgren, and R. A. Slepecky. Inhibition of the development of the spore septum and membranes in Bacillus cereus by beta-phenethyl alcohol. J. Bacteriol. 91:324-331. 1966.-The effect of phenethyl alcohol (PEA) upon the initial stages of sporulation in Bacillus...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.1.324-331.1966

    authors: Remsen CC,Lundgren DG,Slepecky RA

    更新日期:1966-01-01 00:00:00

  • Role of lithium ions in proline transport in Escherichia coli.

    abstract::Mechanisms of Li+ stimulation of proline transport were studied in cells of Escherichia coli 7 and NR70, a mutant of strain 7 lacking adenosine triphosphatase (EC 3.6.1.3). An electrochemical potential difference of Li+ induced in an inward direction of energy-depleted cells caused a transient uptake of proline depend...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.2.560-564.1979

    authors: Kayama-Gonda Y,Kawasaki T

    更新日期:1979-08-01 00:00:00

  • ORGANIC REQUIREMENTS AND SYNTHETIC MEDIA FOR GROWTH OF THE MYXOMYCETE PHYSARUM POLYCEPHALUM.

    abstract::Daniel, John W. (University of Wisconsin, Madison), Karlee L. Babcock, Alice H. Sievert, and Harold P. Rusch. Organic requirements and synthetic media for growth of the myxomycete Physarum polycephalum. J. Bacteriol. 86:324-331. 1963.-An isolate of Physarum polycephalum was maintained in submersed pure culture in a co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.2.324-331.1963

    authors: DANIEL JW,BABCOCK KL,SIEVERT AH,RUSCH HP

    更新日期:1963-08-01 00:00:00

  • A Type Ib ParB protein involved in plasmid partitioning in a gram-positive bacterium.

    abstract::Our current understanding of segregation of prokaryotic plasmids has been derived mainly from the study of the gram-negative bacterial plasmids. We previously reported a replicon of the cryptic plasmid from a gram-positive bacterium, Leifsonia xyli subsp. cynodontis. The replicon contains a putative plasmid partition ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01232-06

    authors: Yin P,Li TY,Xie MH,Jiang L,Zhang Y

    更新日期:2006-12-01 00:00:00

  • Effect of pH on the proportions of polar lipids, in chemostat cultures of Bacillus subtilis.

    abstract::Significant changes in the relative proportions of the individual polar lipids of two strains of Bacillus subtilis were observed when the pH of their chemostat cultures was varied. In phosphate- and magnesium-limited cultures of B. subtilis var. niger NCIB 8058. lysylphosphatidylglycerol was present in higher proporti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.3.999-1003.1974

    authors: Minnikin DE,Abdolrahimzadeh H

    更新日期:1974-12-01 00:00:00

  • Molecular cloning of structural and immunity genes for megacins A-216 and A-19213 in Bacillus megaterium.

    abstract::A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.3.854-859.1984

    authors: Von Tersch MA,Carlton BC

    更新日期:1984-12-01 00:00:00

  • The plasmid-encoded urease gene cluster of the family Enterobacteriaceae is positively regulated by UreR, a member of the AraC family of transcriptional activators.

    abstract::Ureolytic clinical isolates of Providencia stuartii, Salmonella spp., and some Escherichia coli strains contain large urease-encoding plasmids. Expression of urease activity from these isolates is induced at least 20-fold by urea. In order to facilitate studies on the regulatory mechanism controlling this urea-inducib...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.11.3459-3467.1993

    authors: D'Orazio SE,Collins CM

    更新日期:1993-06-01 00:00:00

  • Comparative studies of lipoteichoic acids from several Bacillus strains.

    abstract::Structural studies were carried out on lipoteichoic acids obtained from defatted cells of 10 Bacillus strains by phenol-water partition followed by chromatography on DEAE-Sephacel and Octyl-Sepharose columns. A group of the tested bacteria (group A), Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus, was...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.2.508-516.1986

    authors: Iwasaki H,Shimada A,Ito E

    更新日期:1986-08-01 00:00:00

  • Activation of protease-constitutive recA proteins of Escherichia coli by rRNA and tRNA.

    abstract::The RecA proteins of the unusually strong protease-constitutive mutants recA1202 and recA1211 can use RNA in addition to single-stranded DNA (ssDNA) as a cofactor in the cleavage of the LexA repressor in vitro. In the presence of rRNA or tRNA, the effectiveness of these proteins decreased in the order RecA1202 greater...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.10.4823-4827.1988

    authors: Wang WB,Tessman ES,Tessman I

    更新日期:1988-10-01 00:00:00

  • Use of polymyxin B, levallorphan, and tetracaine to isolate novel envelope mutants of Escherichia coli.

    abstract::Mutants of Escherichia coli were isolated by their resistance to the bacteriocidal effects of the membrane-active drugs polymyxin B, levallorphan, and tetracaine. The mutants were examined for additional changes in cellular physiology evoked by the lesions; many polymyxin-resistant strains had a concomitant increased ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.2.961-972.1976

    authors: Dame JB,Shapiro BM

    更新日期:1976-08-01 00:00:00

  • Diversity among clinical isolates of Histoplasma capsulatum detected by polymerase chain reaction with arbitrary primers.

    abstract::Clinical isolates of the fungal respiratory and systemic pathogen Histoplasma capsulatum have been placed in several different classes by using genomic restriction fragment length polymorphisms (RFLPs), but in general have not been distinguished further. We report here that a polymerase chain reaction (PCR)-based DNA ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.22.7075-7079.1992

    authors: Kersulyte D,Woods JP,Keath EJ,Goldman WE,Berg DE

    更新日期:1992-11-01 00:00:00

  • Physiological effects of growth of an Escherichia coli temperature-sensitive dnaZ mutant at nonpermissive temperatures.

    abstract::The physiological effects of incubation at nonpermissive temperatures of Escherichia coli mutants that carry a temperature-sensitive dnaZ allele [dnaZ(Ts)2016] were examined. The temperature at which the dnaZ(Ts) protein becomes inactivated in vivo was investigated by measurements of deoxyribonucleic acid (DNA) synthe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.1.151-158.1977

    authors: Chu H,Malone MM,Haldenwang WG,Walker JR

    更新日期:1977-10-01 00:00:00

  • A study of the double mutation of dnaJ and cbpA, whose gene products function as molecular chaperones in Escherichia coli.

    abstract::The CbpA protein is an analog of the DnaJ molecular chaperone of Escherichia coli. To gain insight into the function of CbpA, we examined the nature of a cbpA null mutation with special reference to those of dnaK and dnaJ null mutations. In particular, the cbpA dnaJ double-null mutant was found to exhibit severe defec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.13.3894-3896.1995

    authors: Ueguchi C,Shiozawa T,Kakeda M,Yamada H,Mizuno T

    更新日期:1995-07-01 00:00:00

  • Electron micrography study of the asci and ascospores of Metschnikowia Kamienski.

    abstract::Internal and surface structures of asci and ascospores were studied by transmission electron microscopy (TEM) and by scanning electron microscopy (SEM) to establish the character and number of ascospores within the ascus of Metschnikowia krissii. Enzyme digestion with snail gut enzymes and SEM examination suggested th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.1.316-322.1973

    authors: Talens LT,Miller MW,Miranda M

    更新日期:1973-07-01 00:00:00